AB224064

Anti-SLCO1B3/OATP1B3 antibody

Name: Anti-SLCO1B3/OATP1B3 antibody (ab224064) | Abcam
Brand: Abcam Limited
SKU: AB224064

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(4 Publications)

Rabbit Polyclonal SLCO1B3/OATP1B3 antibody. Suitable for IHC-P, ICC/IF and reacts with Human samples. Cited in 4 publications. Immunogen corresponding to Recombinant Fragment Protein within Human SLCO1B3 aa 650 to C-terminus.

View Alternative Names

LST2, OATP1B3, OATP8, SLC21A8, SLCO1B3, Solute carrier organic anion transporter family member 1B3, Liver-specific organic anion transporter 2, Organic anion transporter 8, Organic anion-transporting polypeptide 8, Solute carrier family 21 member 8, LST-2, OATP-8

6 Images

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SLCO1B3/OATP1B3 antibody (AB224064)

IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SLCO1B3/OATP1B3 antibody (AB224064)

Paraffin embedded human kidney tissue stained for SLCO1B3/OATP1B3 with ab224064 (1/1000 dilution) in immunohistochemical analysis. Negative control - no positive staining in kidney tissue.

IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SLCO1B3/OATP1B3 antibody (AB224064)

Immunohistochemical analysis of human kidney tissue labeling SLCO1B3/OATP1B3 with ab224064 at 1/500 dilution. No positivity as expected.

Performed heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SLCO1B3/OATP1B3 antibody (AB224064)

Immunohistochemical analysis of human skin tissue labeling SLCO1B3/OATP1B3 with ab224064 at 1/500 dilution. No positivity as expected.

Performed heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

Immunocytochemistry/ Immunofluorescence - Anti-SLCO1B3/OATP1B3 antibody (AB224064)

ICC/IF

Supplier Data

Immunocytochemistry/ Immunofluorescence - Anti-SLCO1B3/OATP1B3 antibody (AB224064)

Immunofluorescent analysis of A431 (human epidermoid carcinoma cell line) cells labeling SLCO1B3/OATP1B3 (green) in the plasma membrane with ab224064 at 2 μg/ml.

IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SLCO1B3/OATP1B3 antibody (AB224064)

Immunohistochemical analysis of human liver tissue labeling SLCO1B3/OATP1B3 in the membrane of hepatocytes with ab224064 at 1/500 dilution.

Performed heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SLCO1B3/OATP1B3 antibody (AB224064)

Immunohistochemical analysis of human cerebral cortex tissue labeling SLCO1B3/OATP1B3 with ab224064 at 1/500 dilution. No positivity as expected.

Performed heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

Key facts

Host species

Rabbit

Clonality

Polyclonal

Isotype

IgG

Carrier free

Reacts with

Human

Applications

ICC/IF, IHC-P

applications

Immunogen

Recombinant Fragment Protein within Human SLCO1B3 aa 650 to C-terminus. The exact immunogen used to generate this antibody is proprietary information.

Q9NPD5

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"}, "ICCIF" : {"fullname" : "Immunocytochemistry/ Immunofluorescence", "shortname":"ICC/IF"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "1/500 - 1/1000", "IHCP-species-notes": "<p></p> Perform heat-mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.", "ICCIF-species-checked": "testedAndGuaranteed", "ICCIF-species-dilution-info": "0.25-2 µg/mL", "ICCIF-species-notes": "<p>Fixation/Permeabilization: PFA/Triton X-100.</p>" } } }

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Properties and storage information

Form

Liquid

Purification technique

Affinity purification Immunogen

Storage buffer

pH: 7.2 Preservative: 0.02% Sodium azide Constituents: PBS, 40% Glycerol (glycerin, glycerine)

Shipped at conditions

Blue Ice

Appropriate short-term storage duration

1-2 weeks

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

-20°C

Aliquoting information

Upon delivery aliquot

Storage information

Avoid freeze / thaw cycle

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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

SLCO1B3 also known as OATP1B3 is an organic anion transporting polypeptide with a mass of approximately 84 kDa. It functions as a transporter involved in the uptake of various endogenous and exogenous compounds. Mechanically OATP1B3 facilitates the movement of bile acids hormones and drugs across the basolateral membrane in hepatocytes. It is expressed mainly in the liver where it plays a significant role in drug metabolism and disposition.

Biological function summary

OATP1B3 impacts several physiological processes notably the clearance and systemic availability of drugs and endogenous compounds. It is not considered part of a larger protein complex but works closely with other transporters to regulate the hepatobiliary system. Through its selective transport of substrates OATP1B3 contributes to maintaining homeostasis and proper liver function ensuring efficient detoxification and excretion.

Pathways

OATP1B3 plays a role in the enterohepatic circulation of bile acids and drug metabolism pathways. The transporter influences the pharmacokinetics of many drugs and interacts with related proteins such as CYP3A4 which metabolizes these substrates following their uptake. By mediating hepatic uptake OATP1B3 serves as a gatekeeper within the drug metabolism and disposition process managing entry into the liver for further processing by these enzyme systems.

OATP1B3 has associations with liver-related conditions such as cholestasis and liver cancer. Impaired function or expression of OATP1B3 can lead to altered pharmacokinetics contributing to drug-induced liver injury. Additionally it interacts with proteins like MRP2 which is involved in the excretion of conjugated bile acids. Understanding the role of OATP1B3 in these diseases provides insights into potential therapeutic strategies and drug development.

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:

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Target data

Mediates the Na(+)-independent uptake of organic anions (PubMed : 10779507, PubMed : 15159445, PubMed : 17412826). Shows broad substrate specificity, can transport both organic anions such as bile acid taurocholate (cholyltaurine) and conjugated steroids (17-beta-glucuronosyl estradiol, dehydroepiandrosterone sulfate (DHEAS), and estrone 3-sulfate), as well as eicosanoid leukotriene C4, prostaglandin E2 and L-thyroxine (T4) (PubMed : 10779507, PubMed : 11159893, PubMed : 12568656, PubMed : 15159445, PubMed : 17412826, PubMed : 19129463). Hydrogencarbonate/HCO3(-) acts as the probable counteranion that exchanges for organic anions (PubMed : 19129463). Shows a pH-sensitive substrate specificity towards sulfated steroids, taurocholate and T4 which may be ascribed to the protonation state of the binding site and leads to a stimulation of substrate transport in an acidic microenvironment (PubMed : 19129463). Involved in the clearance of bile acids and organic anions from the liver (PubMed : 22232210). Can take up bilirubin glucuronides from plasma into the liver, contributing to the detoxification-enhancing liver-blood shuttling loop (PubMed : 22232210). Transports coproporphyrin I and III, by-products of heme synthesis, and may be involved in their hepatic disposition (PubMed : 26383540). May contribute to regulate the transport of organic compounds in testes across the blood-testis-barrier (Probable). Can transport HMG-CoA reductase inhibitors (also known as statins) such as pitavastatin, a clinically important class of hypolipidemic drugs (PubMed : 15159445). May play an important role in plasma and tissue distribution of the structurally diverse chemotherapeutic drugs methotrexate and paclitaxel (PubMed : 23243220). May also transport antihypertension agents, such as the angiotensin-converting enzyme (ACE) inhibitor prodrug enalapril, and the highly selective angiotensin II AT1-receptor antagonist valsartan, in the liver (PubMed : 16624871, PubMed : 16627748).

See full target information SLCO1B3

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Publications (4)

Recent publications for all applications. Explore the full list and refine your search

Heliyon 10:e30104 PubMed38720699

2024

Microcystin-leucine arginine induces the proliferation of cholangiocytes and cholangiocarcinoma cells through the activation of the Wnt/β-catenin signaling pathway.

Applications

Unspecified application

Species

Unspecified reactive species

Suppakrit Kongsintaweesuk,Sirinapha Klungsaeng,Kitti Intuyod,Anchalee Techasen,Chawalit Pairojkul,Vor Luvira,Somchai Pinlaor,Porntip Pinlaor

Scientific reports 11:13866 PubMed34230586

2021

Genome-wide CRISPR/Cas9 screening in human iPS derived cardiomyocytes uncovers novel mediators of doxorubicin cardiotoxicity.

Applications

Unspecified application

Species

Unspecified reactive species

Valerie Sapp,Aitor Aguirre,Gayatri Mainkar,Jeffrey Ding,Eric Adler,Ronglih Liao,Sonia Sharma,Mohit Jain

Frontiers in oncology 11:626883 PubMed33869009

2021

Identification of IFN-Induced Transmembrane Protein 1 With Prognostic Value in Pancreatic Cancer Using Network Module-Based Analysis.

Applications

Unspecified application

Species

Unspecified reactive species

Lingyun Wu,Xinli Zhu,Danfang Yan,Mengmeng Tang,Chiyuan Ma,Senxiang Yan

The Journal of pharmacology and experimental therapeutics 376:148-160 PubMed33168642

2020

Transport Properties of Statins by Organic Anion Transporting Polypeptide 1A2 and Regulation by Transforming Growth Factor- Signaling in Human Endothelial Cells.

Applications

Unspecified application

Species

Unspecified reactive species

Patrick T Ronaldson,Hrvoje Brzica,Wazir Abdullahi,Bianca G Reilly,Thomas P Davis

View all publications

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Product promise

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Anti-SLCO1B3/OATP1B3 antibody

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SLCO1B3/OATP1B3 antibody (AB224064)

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SLCO1B3/OATP1B3 antibody (AB224064)

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SLCO1B3/OATP1B3 antibody (AB224064)

Immunocytochemistry/ Immunofluorescence - Anti-SLCO1B3/OATP1B3 antibody (AB224064)

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SLCO1B3/OATP1B3 antibody (AB224064)

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SLCO1B3/OATP1B3 antibody (AB224064)

Key facts

Host species

Clonality

Isotype

Carrier free

Reacts with

Applications

Immunogen

Alternative Products

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Complementary Products

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Secondary Antibodies

Primary Antibodies

Primary Antibodies

Reactivity data

Properties and storage information

Form

Purification technique

Storage buffer

Shipped at conditions

Appropriate short-term storage duration

Appropriate short-term storage conditions

Appropriate long-term storage conditions

Aliquoting information

Storage information

Supplementary information

Biological function summary

Pathways

Product protocols

Target data

Publications (4)

Product promise