Skip to main content

Rabbit Polyclonal SLCO1B3/OATP1B3 antibody. Suitable for IHC-P, ICC/IF and reacts with Human samples. Cited in 2 publications. Immunogen corresponding to Recombinant Fragment Protein within Human SLCO1B3 aa 650 to C-terminus.

Be the first to review this product! Submit a review

Images

Key facts

Isotype

IgG

Host species

Rabbit

Storage buffer

pH: 7.2
Preservative: 0.02% Sodium azide
Constituents: PBS, 40% Glycerol (glycerin, glycerine)

Form

Liquid

Clonality

Polyclonal

Immunogen

  • Recombinant Fragment Protein within Human SLCO1B3 aa 650 to C-terminus. The exact immunogen used to generate this antibody is proprietary information. Database link Q9NPD5
Loading...
Loading...
Loading...
Loading...
Loading...
Loading...
Loading...
Loading...

Reactivity data

Select an application
Product promiseTestedExpectedPredictedNot recommended
IHC-PICC/IF
Human
Tested
Tested

Tested
Tested

Species

Human

Dilution info

1/500.00000 - 1/1000.00000

Notes

Perform heat-mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

Tested
Tested

Species

Human

Dilution info

0.25000-2.00000 µg/mL

Notes

Fixation/Permeabilization: PFA/Triton X-100.

Target data

Function

Mediates the Na(+)-independent uptake of organic anions such as 17-beta-glucuronosyl estradiol, taurocholate, triiodothyronine (T3), leukotriene C4, dehydroepiandrosterone sulfate (DHEAS), methotrexate and sulfobromophthalein (BSP). Involved in the clearance of bile acids and organic anions from the liver.

Alternative names

Recommended products

  1. Loading...
  2. Loading...
  3. Loading...
  4. Loading...

Rabbit Polyclonal SLCO1B3/OATP1B3 antibody. Suitable for IHC-P, ICC/IF and reacts with Human samples. Cited in 2 publications. Immunogen corresponding to Recombinant Fragment Protein within Human SLCO1B3 aa 650 to C-terminus.

Alternative names

Key facts

Isotype

IgG

Form

Liquid

Clonality

Polyclonal

Immunogen
  • Recombinant Fragment Protein within Human SLCO1B3 aa 650 to C-terminus. The exact immunogen used to generate this antibody is proprietary information. Database link Q9NPD5
Purification technique

Affinity purification Immunogen

Concentration
Loading...

Storage

Shipped at conditions

Blue Ice

Appropriate short-term storage duration

1-2 weeks

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

-20°C

Aliquoting information

Upon delivery aliquot

Storage information

Avoid freeze / thaw cycle

Notes

Abcam is leading the way to address reproducibility in scientific research with our highly validated recombinant monoclonal and recombinant multiclonal antibodies. Search & select one of Abcam's thousands of recombinant alternatives to eliminate batch-variability and unnecessary animal use.

If you do not find a host species to meet your needs, our catalogue and custom Chimeric range provides scientists the specificity of Abcam's RabMAbs in the species backbone of your choice. Remember to also review our range of edited cell lines, proteins and biochemicals relevant to your target that may help you further your research goals.

Abcam antibodies are extensively validated in a wide range of species and applications, so please check the reagent specifications meet your scientific needs before purchasing. If you have any questions or bespoke requirements, simply visit the Contact Us page to send us an inquiry or contact our Support Team ahead of purchase.

Supplementary info

Biological function summary

OATP1B3 impacts several physiological processes notably the clearance and systemic availability of drugs and endogenous compounds. It is not considered part of a larger protein complex but works closely with other transporters to regulate the hepatobiliary system. Through its selective transport of substrates OATP1B3 contributes to maintaining homeostasis and proper liver function ensuring efficient detoxification and excretion.

Activity summary

SLCO1B3 also known as OATP1B3 is an organic anion transporting polypeptide with a mass of approximately 84 kDa. It functions as a transporter involved in the uptake of various endogenous and exogenous compounds. Mechanically OATP1B3 facilitates the movement of bile acids hormones and drugs across the basolateral membrane in hepatocytes. It is expressed mainly in the liver where it plays a significant role in drug metabolism and disposition.

Pathways

OATP1B3 plays a role in the enterohepatic circulation of bile acids and drug metabolism pathways. The transporter influences the pharmacokinetics of many drugs and interacts with related proteins such as CYP3A4 which metabolizes these substrates following their uptake. By mediating hepatic uptake OATP1B3 serves as a gatekeeper within the drug metabolism and disposition process managing entry into the liver for further processing by these enzyme systems.

Associated diseases and disorders

OATP1B3 has associations with liver-related conditions such as cholestasis and liver cancer. Impaired function or expression of OATP1B3 can lead to altered pharmacokinetics contributing to drug-induced liver injury. Additionally it interacts with proteins like MRP2 which is involved in the excretion of conjugated bile acids. Understanding the role of OATP1B3 in these diseases provides insights into potential therapeutic strategies and drug development.

Product promise

We are dedicated to supporting your work with high quality reagents and we are here for you every step of the way should you need us.

In the unlikely event of one of our products not working as expected, you are covered by our product promise.

Full details and terms and conditions can be found here:
Terms & Conditions.

6 product images

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SLCO1B3/OATP1B3 antibody (ab224064), expandable thumbnail

    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SLCO1B3/OATP1B3 antibody (ab224064)

    Immunohistochemical analysis of human liver tissue labeling SLCO1B3/OATP1B3 in the membrane of hepatocytes with ab224064 at 1/500 dilution.

    Performed heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

  • Immunocytochemistry/ Immunofluorescence - Anti-SLCO1B3/OATP1B3 antibody (ab224064), expandable thumbnail

    Immunocytochemistry/ Immunofluorescence - Anti-SLCO1B3/OATP1B3 antibody (ab224064)

    Immunofluorescent analysis of A431 (human epidermoid carcinoma cell line) cells labeling SLCO1B3/OATP1B3 (green) in the plasma membrane with ab224064 at 2 μg/ml.

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SLCO1B3/OATP1B3 antibody (ab224064), expandable thumbnail

    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SLCO1B3/OATP1B3 antibody (ab224064)

    Immunohistochemical analysis of human cerebral cortex tissue labeling SLCO1B3/OATP1B3 with ab224064 at 1/500 dilution. No positivity as expected.

    Performed heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SLCO1B3/OATP1B3 antibody (ab224064), expandable thumbnail

    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SLCO1B3/OATP1B3 antibody (ab224064)

    Immunohistochemical analysis of human skin tissue labeling SLCO1B3/OATP1B3 with ab224064 at 1/500 dilution. No positivity as expected.

    Performed heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SLCO1B3/OATP1B3 antibody (ab224064), expandable thumbnail

    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SLCO1B3/OATP1B3 antibody (ab224064)

    Immunohistochemical analysis of human kidney tissue labeling SLCO1B3/OATP1B3 with ab224064 at 1/500 dilution. No positivity as expected.

    Performed heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SLCO1B3/OATP1B3 antibody (ab224064), expandable thumbnail

    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SLCO1B3/OATP1B3 antibody (ab224064)

    Paraffin embedded human kidney tissue stained for SLCO1B3/OATP1B3 with ab224064 (1/1000 dilution) in immunohistochemical analysis. Negative control - no positive staining in kidney tissue.

Downloads

Product protocols

For this product, it's our understanding that no specific protocols are required. You can:

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com

There was a problem

We can’t download that datasheet. Please try again. If you need help, contact our Customer Services team at technical@abcam.com