Rabbit Polyclonal SLCO1B3/OATP1B3 antibody. Suitable for IHC-P, ICC/IF and reacts with Human samples. Cited in 2 publications. Immunogen corresponding to Recombinant Fragment Protein within Human SLCO1B3 aa 650 to C-terminus.
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Publications
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- Scientific reports 11:138662021Genome-wide CRISPR/Cas9 screening in human iPS derived cardiomyocytes uncovers novel mediators of doxorubicin cardiotoxicity.Applications:Unspecified applicationReactive species:Unspecified reactive speciesValerie Sapp,Aitor Aguirre,Gayatri Mainkar,Jeffrey Ding,Eric Adler,Ronglih Liao,Sonia Sharma,Mohit JainPubMed 34230586
- The Journal of pharmacology and experimental therapeutics 376:148-1602020Transport Properties of Statins by Organic Anion Transporting Polypeptide 1A2 and Regulation by Transforming Growth Factor- Signaling in Human Endothelial Cells.Applications:Unspecified applicationReactive species:Unspecified reactive speciesPatrick T Ronaldson,Hrvoje Brzica,Wazir Abdullahi,Bianca G Reilly,Thomas P DavisPubMed 33168642
Key facts
- Isotype
IgG
- Host species
Rabbit
- Storage buffer
pH: 7.2
Preservative: 0.02% Sodium azide
Constituents: PBS, 40% Glycerol (glycerin, glycerine)- Form
Liquid
- Clonality
Polyclonal
Immunogen
- Recombinant Fragment Protein within Human SLCO1B3 aa 650 to C-terminus. The exact immunogen used to generate this antibody is proprietary information. Database link Q9NPD5
Reactivity data
Select an application| IHC-P | ICC/IF | |
|---|---|---|
| Human | Tested | Tested |
IHC-P
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info 1/500.00000 - 1/1000.00000 | Notes Perform heat-mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol. |
ICC/IF
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info 0.25000-2.00000 µg/mL | Notes Fixation/Permeabilization: PFA/Triton X-100. |
Associated Products
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Target data
Function
Mediates the Na(+)-independent uptake of organic anions such as 17-beta-glucuronosyl estradiol, taurocholate, triiodothyronine (T3), leukotriene C4, dehydroepiandrosterone sulfate (DHEAS), methotrexate and sulfobromophthalein (BSP). Involved in the clearance of bile acids and organic anions from the liver.
Alternative names
Solute carrier organic anion transporter family member 1B3, Liver-specific organic anion transporter 2, Organic anion transporter 8, Organic anion-transporting polypeptide 8, Solute carrier family 21 member 8, LST-2, OATP-8, SLCO1B3, LST2, OATP1B3, SLC21A8, OATP8
Recommended products
Rabbit Polyclonal SLCO1B3/OATP1B3 antibody. Suitable for IHC-P, ICC/IF and reacts with Human samples. Cited in 2 publications. Immunogen corresponding to Recombinant Fragment Protein within Human SLCO1B3 aa 650 to C-terminus.
Alternative names
Solute carrier organic anion transporter family member 1B3, Liver-specific organic anion transporter 2, Organic anion transporter 8, Organic anion-transporting polypeptide 8, Solute carrier family 21 member 8, LST-2, OATP-8, SLCO1B3, LST2, OATP1B3, SLC21A8, OATP8
Key facts
- Isotype
IgG
- Host species
Rabbit
- Storage buffer
pH: 7.2
Preservative: 0.02% Sodium azide
Constituents: PBS, 40% Glycerol (glycerin, glycerine)- Form
Liquid
- Clonality
Polyclonal
- Immunogen
- Recombinant Fragment Protein within Human SLCO1B3 aa 650 to C-terminus. The exact immunogen used to generate this antibody is proprietary information. Database link Q9NPD5
- Purification technique
Affinity purification Immunogen
- Concentration
Storage
- Shipped at conditions
Blue Ice
- Appropriate short-term storage duration
1-2 weeks
- Appropriate short-term storage conditions
+4°C
- Appropriate long-term storage conditions
-20°C
- Aliquoting information
Upon delivery aliquot
- Storage information
Avoid freeze / thaw cycle
Notes
Abcam is leading the way to address reproducibility in scientific research with our highly validated recombinant monoclonal and recombinant multiclonal antibodies. Search & select one of Abcam's thousands of recombinant alternatives to eliminate batch-variability and unnecessary animal use.
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Abcam antibodies are extensively validated in a wide range of species and applications, so please check the reagent specifications meet your scientific needs before purchasing. If you have any questions or bespoke requirements, simply visit the Contact Us page to send us an inquiry or contact our Support Team ahead of purchase.
Supplementary info
This supplementary information is collated from multiple sources and compiled automatically.
- Activity summary
SLCO1B3 also known as OATP1B3 is an organic anion transporting polypeptide with a mass of approximately 84 kDa. It functions as a transporter involved in the uptake of various endogenous and exogenous compounds. Mechanically OATP1B3 facilitates the movement of bile acids hormones and drugs across the basolateral membrane in hepatocytes. It is expressed mainly in the liver where it plays a significant role in drug metabolism and disposition.
- Biological function summary
OATP1B3 impacts several physiological processes notably the clearance and systemic availability of drugs and endogenous compounds. It is not considered part of a larger protein complex but works closely with other transporters to regulate the hepatobiliary system. Through its selective transport of substrates OATP1B3 contributes to maintaining homeostasis and proper liver function ensuring efficient detoxification and excretion.
- Pathways
OATP1B3 plays a role in the enterohepatic circulation of bile acids and drug metabolism pathways. The transporter influences the pharmacokinetics of many drugs and interacts with related proteins such as CYP3A4 which metabolizes these substrates following their uptake. By mediating hepatic uptake OATP1B3 serves as a gatekeeper within the drug metabolism and disposition process managing entry into the liver for further processing by these enzyme systems.
- Associated diseases and disorders
OATP1B3 has associations with liver-related conditions such as cholestasis and liver cancer. Impaired function or expression of OATP1B3 can lead to altered pharmacokinetics contributing to drug-induced liver injury. Additionally it interacts with proteins like MRP2 which is involved in the excretion of conjugated bile acids. Understanding the role of OATP1B3 in these diseases provides insights into potential therapeutic strategies and drug development.
Product promise
Tested
We have tested this species and application combination and it works. It is covered by our product promise.
Expected
We have not tested this specific species and application combination in-house, but expect it will work. It is covered by our product promise.
Predicted
This species and application combination has not been tested, but we predict it will work based on strong homology. However, this combination is not covered by our product promise.
Not recommended
We do not recommend this combination. It is not covered by our product promise.
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6 product images
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SLCO1B3/OATP1B3 antibody (ab224064)
Immunohistochemical analysis of human liver tissue labeling SLCO1B3/OATP1B3 in the membrane of hepatocytes with ab224064 at 1/500 dilution.
Performed heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
Immunocytochemistry/ Immunofluorescence - Anti-SLCO1B3/OATP1B3 antibody (ab224064)
Immunofluorescent analysis of A431 (human epidermoid carcinoma cell line) cells labeling SLCO1B3/OATP1B3 (green) in the plasma membrane with ab224064 at 2 μg/ml.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SLCO1B3/OATP1B3 antibody (ab224064)
Immunohistochemical analysis of human cerebral cortex tissue labeling SLCO1B3/OATP1B3 with ab224064 at 1/500 dilution. No positivity as expected.
Performed heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SLCO1B3/OATP1B3 antibody (ab224064)
Immunohistochemical analysis of human skin tissue labeling SLCO1B3/OATP1B3 with ab224064 at 1/500 dilution. No positivity as expected.
Performed heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SLCO1B3/OATP1B3 antibody (ab224064)
Immunohistochemical analysis of human kidney tissue labeling SLCO1B3/OATP1B3 with ab224064 at 1/500 dilution. No positivity as expected.
Performed heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SLCO1B3/OATP1B3 antibody (ab224064)
Paraffin embedded human kidney tissue stained for SLCO1B3/OATP1B3 with ab224064 (1/1000 dilution) in immunohistochemical analysis. Negative control - no positive staining in kidney tissue.
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