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AB313384

Anti-SLCO2B1/OATP2B1 antibody [EPR28179-63]

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Anti-SLCO2B1/OATP2B1 antibody [EPR28179-63] is a Rabbit Monoclonal antibody that is used in SLCO2B1/OATP2B1 IP, Western Blot. Suitable for Mouse, Rat samples.

SLCO2B1, also known as OATP2B1, is an organic anion transporter involved in the uptake and disposition of various compounds. In cancer research, SLCO2B1 has been linked to prostate cancer progression, with increased expression observed in castration-resistant prostate cancer (CRPC). Genetic variants of SLCO2B1 can affect androgen uptake, influencing cancer outcomes and therapy responses, making it a significant focus in cancer treatment strategies.

View Alternative Names

Oatp2b1, Slc21a9, Slco2b1, Solute carrier organic anion transporter family member 2B1, Organic anion transporting polypeptide 2B1, Solute carrier family 21 member 9, OATP2B1

4 Images
Immunoprecipitation - Anti-SLCO2B1/OATP2B1 antibody [EPR28179-63] (AB313384)
  • IP

Supplier Data

Immunoprecipitation - Anti-SLCO2B1/OATP2B1 antibody [EPR28179-63] (AB313384)

SLCO2B1/OATP2B1 was immunoprecipitated from 0.35 mg Mouse liver tissue lysate with ab313384 at 1/30 dilution (2ug in 0.35mg lysates). Western blot was performed on the immunoprecipitate using ab313384 at 1/1000 dilution. VeriBlot for IP secondary antibody (HRP) (ab131366) was used at 1/5000 dilution.

Lane 1 : Mouse liver tissue lysate
Lane 2 : ab313384 IP in Mouse liver tissue lysate
Lane 3 : Rabbit monoclonal IgG (ab172730) instead of ab313384 in Mouse liver tissue lysate

Blocking and dilution buffer and concentration : 5% NFDM/TBST.

Exposure time : 23 seconds

All lanes:

Immunoprecipitation - Anti-SLCO2B1/OATP2B1 antibody [EPR28179-63] (ab313384) at 1/1000 dilution

All lanes:

Mouse liver tissue lysate

Secondary

All lanes:

Immunoprecipitation - VeriBlot for IP Detection Reagent (HRP) (<a href='/en-us/products/reagents/veriblot-for-ip-detection-reagent-hrp-ab131366'>ab131366</a>) at 1/5000 dilution

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Western blot - Anti-SLCO2B1/OATP2B1 antibody [EPR28179-63] (AB313384)
  • WB

Supplier Data

Western blot - Anti-SLCO2B1/OATP2B1 antibody [EPR28179-63] (AB313384)

Blocking and diluting buffer and concentration : 5% NFDM/TBST Low expression : kidney, skeletal muscle tissue (PMID : 11159893) Samples are non-boiled as boiling may cause protein aggregation. The expression profile/ molecular weight observed is consistent with what has been described in the literature (PMID : 11159893; PMID : 17020956). In Western blot, anti-GAPDH antibody (ab181602) loading control staining at 1/200000 dilution. Exposure time : 48 seconds

All lanes:

Western blot - Anti-SLCO2B1/OATP2B1 antibody [EPR28179-63] (ab313384) at 1/1000 dilution

Lane 1:

Mouse liver tissue lysate at 20 µg

Lane 2:

Mouse kidney tissue lysate at 20 µg

Lane 3:

Mouse skeletal muscle tissue lysate at 20 µg

Secondary

All lanes:

Western blot - Goat Anti-Rabbit IgG H&L (HRP) (<a href='/en-us/products/secondary-antibodies/goat-rabbit-igg-h-l-hrp-ab97051'>ab97051</a>) at 1/100000 dilution

Observed band size: 90 kDa

false

Exposure time: 48s

Western blot - Anti-SLCO2B1/OATP2B1 antibody [EPR28179-63] (AB313384)
  • WB

Supplier Data

Western blot - Anti-SLCO2B1/OATP2B1 antibody [EPR28179-63] (AB313384)

Blocking and diluting buffer and concentration : 5% NFDM/TBST In Western blot, anti-GAPDH antibody (ab181602) loading control staining at 1/200000 dilution. Exposure time : 59 seconds

All lanes:

Western blot - Anti-SLCO2B1/OATP2B1 antibody [EPR28179-63] (ab313384) at 1/1000 dilution

Lane 1:

Untreated Mouse liver tissue lysate at 20 µg

Lane 2:

Mouse liver tissue lysate treated with Protein treated with Peptide:N-glycosidase F (PNGase F) at 20 µg

Secondary

All lanes:

Western blot - Goat Anti-Rabbit IgG H&L (HRP) (<a href='/en-us/products/secondary-antibodies/goat-rabbit-igg-h-l-hrp-ab97051'>ab97051</a>) at 1/100000 dilution

Observed band size: 90 kDa

false

Exposure time: 59s

Western blot - Anti-SLCO2B1/OATP2B1 antibody [EPR28179-63] (AB313384)
  • WB

Supplier Data

Western blot - Anti-SLCO2B1/OATP2B1 antibody [EPR28179-63] (AB313384)

Blocking and diluting buffer and concentration : 5% NFDM/TBST Low expression : testis (PMID : 11159893) Lysates were freshly made and used immediately to minimize protein degradation. Samples are non-boiled as boiling may cause protein aggregation. In Western blot, anti-GAPDH antibody (ab181602) loading control staining at 1/200000 dilution. Exposure time : Lane 1 : 26 seconds Lane 2, 3 : 147 seconds Lane 4, 5 : 37 seconds

All lanes:

Western blot - Anti-SLCO2B1/OATP2B1 antibody [EPR28179-63] (ab313384) at 1/1000 dilution

Lane 1:

Rat liver tissue lysate at 20 µg

Lane 2:

Rat testis tissue lysate at 20 µg

Lane 3:

Rat spleen tissue lysate at 20 µg

Lane 4:

Mouse liver tissue lysate at 20 µg

Lane 5:

Mouse testis tissue lysate at 20 µg

Secondary

All lanes:

Western blot - Goat Anti-Rabbit IgG H&L (HRP) (<a href='/en-us/products/secondary-antibodies/goat-rabbit-igg-h-l-hrp-ab97051'>ab97051</a>) at 1/100000 dilution

Observed band size: 90 kDa

false

  • Carrier free

    Anti-SLCO2B1/OATP2B1 antibody [EPR28179-63] - BSA and Azide free

Key facts

Host species

Rabbit

Clonality

Monoclonal

Clone number

EPR28179-63

Isotype

IgG

Carrier free

No

Reacts with

Mouse, Rat

Applications

WB, IP

applications

Immunogen

The exact immunogen used to generate this antibody is proprietary information.

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Reactivity data

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Product details

Patented technology
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:

  • - High batch-to-batch consistency and reproducibility
  • - Improved sensitivity and specificity
  • - Long-term security of supply
  • - Animal-free batch production

For more information, read more on recombinant antibodies.

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Properties and storage information

Form
Liquid
Purification technique
Affinity purification Protein A
Storage buffer
pH: 7.2 - 7.4 Preservative: 0.01% Sodium azide Constituents: PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA
Shipped at conditions
Conditional Ambient
Appropriate short-term storage duration
1-2 weeks
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
-20°C
Aliquoting information
Upon delivery aliquot
Storage information
Avoid freeze / thaw cycle
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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

The SLCO2B1 also known as OATP2B1 is a member of the organic anion transporting polypeptides family. It functions as a transporter responsible for mediating the uptake of various drugs hormones and other organic compounds. This protein has a molecular mass of about 80 kDa. SLCO2B1 is primarily expressed in the human intestine and liver with significant presence in the brain lung and placenta. It plays a role in the absorption and clearance of pharmacologically relevant compounds.
Biological function summary

SLCO2B1 regulates the transport of endogenous and exogenous substrates across cell membranes. It does not form part of a larger protein complex but interacts with many substrates such as steroid hormones and therapeutic agents. SLCO2B1 influences how drugs are distributed and eliminated affecting the pharmacokinetics and bioavailability in tissues where it is expressed.

Pathways

SLCO2B1 integrates into processes related to drug metabolism and transport. It is important in the absorption and elimination phases within the pharmacokinetic pathway and has a role in the metabolism of steroid hormones. In this context SLCO2B1 cooperates with other transporters and metabolizing enzymes including cytochrome P450s to control the intracellular concentration of various substances.

SLCO2B1 plays a role in influencing drug efficacy and toxicity impacting personalized medicine approaches for individuals with specific genetic transporter variants. SLCO2B1 has implications in cardiovascular diseases related to the pharmacological metabolism of drugs affecting heart rhythm. Additionally its function in hormone regulation connects it with metabolic disorders. In these diseases SLCO2B1 interacts with proteins like MDR1 which also impact drug transport and resistance.
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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:
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Target data

Mediates the Na(+)-independent transport of steroid sulfate conjugates such as estrone 3-sulfate (E1S), dehydroepiandrosterone sulfate (DHEA-S) and pregnenolone sulfate (PregS) and other specific organic anions (PubMed : 31123035). Responsible for the transport of E1S through the basal membrane of syncytiotrophoblast, highlighting a potential role in the placental absorption of fetal-derived sulfated steroids including DHEA-S (By similarity). Also facilitates the uptake of sulfated steroids at the basal/sinusoidal membrane of hepatocytes, therefore accounting for the major part of organic anions clearance of liver. Mediates the intestinal uptake of sulfated steroids. Mediates the uptake of the neurosteroids DHEA-S and PregS into the endothelial cells of the blood-brain barrier as the first step to enter the brain. Also plays a role in the reuptake of neuropeptides such as substance P/TAC1 and vasoactive intestinal peptide/VIP released from retinal neurons. May act as a heme transporter that promotes cellular iron availability. Also transports heme by-product coproporphyrin III (CPIII), and may be involved in their hepatic disposition (By similarity). Mediates the uptake of other substrates such as prostaglandins D2 (PGD2), E1 (PGE1) and E2 (PGE2), taurocholate, L-thyroxine, leukotriene C4 and thromboxane B2 (PubMed : 31123035). May contribute to regulate the transport of organic compounds in testis across the blood-testis-barrier (By similarity). Shows a pH-sensitive substrate specificity which may be ascribed to the protonation state of the binding site and leads to a stimulation of substrate transport in an acidic microenvironment. The exact transport mechanism has not been yet deciphered but most likely involves an anion exchange, coupling the cellular uptake of organic substrate with the efflux of an anionic compound. Hydrogencarbonate/HCO3(-) acts as a probable counteranion that exchanges for organic anions. Cytoplasmic glutamate may also act as counteranion in the placenta. An inwardly directed proton gradient has also been proposed as the driving force of E1S uptake with a (H(+) : E1S) stoichiometry of (1 : 1) (By similarity).
See full target information Slco2b1
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Product promise

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For full details, please see our Terms & Conditions

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