AB101346

Anti-SLD5 antibody

Name: Anti-SLD5 antibody (ab101346) | Abcam
Brand: Abcam Limited
SKU: AB101346
Rating: 1 (1 reviews)

(1 Review)

(4 Publications)

Rabbit Polyclonal SLD5 antibody. Suitable for WB, ICC/IF, IHC-P and reacts with Mouse, Human samples. Cited in 4 publications. Immunogen corresponding to Recombinant Fragment Protein within Human GINS4 aa 1-200.

View Alternative Names

SLD5, GINS4, DNA replication complex GINS protein SLD5, GINS complex subunit 4

4 Images

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SLD5 antibody (AB101346)

IHC-P

Unknown

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SLD5 antibody (AB101346)

ab101346 at 1/500 staining SLD5 in paraffin-embedded human gastric cancer.

Immunocytochemistry/ Immunofluorescence - Anti-SLD5 antibody (AB101346)

ICC/IF

Unknown

Immunocytochemistry/ Immunofluorescence - Anti-SLD5 antibody (AB101346)

ab101346 at 1/200 staining paraformaldehyde-fixed A431 in IF. Bottom image costained with Hoechst 33342.

Unknown

Western blot - Anti-SLD5 antibody (AB101346)

12% SDS PAGE

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Western blot - Anti-SLD5 antibody (ab101346) at 1/1000 dilution

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293T whole cell lysate at 30 µg

Predicted band size: 26 kDa

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Western blot - Anti-SLD5 antibody (AB101346)

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Western blot - Anti-SLD5 antibody (ab101346) at 1/10000 dilution

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Mouse heart at 50 µg

Predicted band size: 26 kDa,28 kDa,59 kDa

false

Key facts

Host species

Rabbit

Clonality

Polyclonal

Isotype

IgG

Carrier free

Reacts with

Mouse, Human

Applications

WB, IHC-P, ICC/IF

applications

Immunogen

Recombinant Fragment Protein within Human GINS4 aa 1-200. The exact immunogen used to generate this antibody is proprietary information.

Q9BRT9

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "ICCIF" : {"fullname" : "Immunocytochemistry/ Immunofluorescence", "shortname":"ICC/IF"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/1000 - 1/10000", "WB-species-notes": "", "ICCIF-species-checked": "testedAndGuaranteed", "ICCIF-species-dilution-info": "1/100 - 1/500", "ICCIF-species-notes": "", "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "1/100 - 1/500", "IHCP-species-notes": "Suggested antigen retrieval using heat mediated 10mM Citrate buffer (pH 6.0) or Tris-EDTA buffer (pH 8.0)." }, "Mouse": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/1000 - 1/10000", "WB-species-notes": "", "ICCIF-species-checked": "guaranteed", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "", "IHCP-species-checked": "guaranteed", "IHCP-species-dilution-info": "", "IHCP-species-notes": "" } } }

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Properties and storage information

Form

Liquid

Purification technique

Affinity purification Immunogen

Storage buffer

pH: 7 Preservative: 0.01% Thimerosal (merthiolate) Constituents: 20% Glycerol (glycerin, glycerine), 1.21% Tris, 0.75% Glycine

Shipped at conditions

Blue Ice

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

-20°C

Aliquoting information

Upon delivery aliquot

Storage information

Avoid freeze / thaw cycle

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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

SLD5 also known as GINS4 is a subunit of the GINS complex. It has a molecular mass of approximately 22 kDa. SLD5 plays a significant role in DNA replication specifically during the initiation and elongation phases. The protein is expressed in various tissues with higher levels observed in proliferating cells. By binding to DNA replication origins SLD5 ensures the proper unwinding and loading of other replication factors.

Biological function summary

SLD5 interacts with other components of the GINS complex to form a stable structure necessary for the replication fork's progression. The GINS complex comprising SLD5 PSF1 PSF2 and PSF3 couples with other protein assemblies like CDC45 and MCM (mini chromosome maintenance) proteins. This dynamic association enables the replication machinery to effectively duplicate the genome without errors.

Pathways

SLD5 plays a part in the initiation of the DNA replication pathway. It partners with proteins like CDC45 and the MCM complex to form a replicative helicase. This assembly serves to unwound the DNA double helix facilitating replication. Furthermore interactions with ATR and ATM pathways position SLD5 within the larger network of DNA damage response ensuring any replication stress is adequately managed.

SLD5 exhibits connections with cancers such as breast cancer and colorectal cancer. Alterations in SLD5 expression can influence tumor progression by modulating cell proliferation rates. Through these pathways proteins like MCM and CDC45 are also implicated serving as key regulators in oncogenic processes where SLD5 activity expands its influence. Understanding these relationships offers insights into potential therapeutic targets for controlling abnormal cell growth.

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

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Target data

Required for correct functioning of the GINS complex, a complex that plays an essential role in the initiation of DNA replication, and progression of DNA replication forks (PubMed : 17417653, PubMed : 28414293). GINS complex is a core component of CDC45-MCM-GINS (CMG) helicase, the molecular machine that unwinds template DNA during replication, and around which the replisome is built (PubMed : 32453425, PubMed : 34694004, PubMed : 34700328, PubMed : 35585232).

See full target information GINS4

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Publications (4)

Recent publications for all applications. Explore the full list and refine your search

Journal of immunology (Baltimore, Md. : 1950) 213:40-51 PubMed38809096

2024

Replication Stress in Activated Human NK Cells Induces Sensitivity to Apoptosis.

Applications

Unspecified application

Species

Unspecified reactive species

Nicole C Guilz,Yong-Oon Ahn,Hijab Fatima,Luis Alberto Pedroza,Seungmae Seo,Rajesh Kumar Soni,Ning Wang,Dieter Egli,Emily M Mace

Frontiers in oncology 11:654185 PubMed33842367

2021

Overexpression of GINS4 Is Associated With Tumor Progression and Poor Survival in Hepatocellular Carcinoma.

Applications

Unspecified application

Species

Unspecified reactive species

Ziying Zhang,Peng Chen,Hui Xie,Peiguo Cao

Journal of cancer research and clinical oncology 145:363-371 PubMed30465075

2018

Induced expression of GINS complex is an essential step for reactivation of quiescent stem-like tumor cells within the peri-necrotic niche in human glioblastoma.

Applications

Unspecified application

Species

Unspecified reactive species

Tokuhiro Kimura,Dan Cui,Hiroo Kawano,Chihiro Yoshitomi-Sakamoto,Nobuyuki Takakura,Eiji Ikeda

Molecular and cellular biology 38: PubMed29061732

2017

Sld5 Ensures Centrosomal Resistance to Congression Forces by Preserving Centriolar Satellites.

Applications

Unspecified application

Species

Unspecified reactive species

Manpreet Kaur,Raksha Devi,Tanushree Ghosh,Md Muntaz Khan,Praveen Kumar, Priyanka,Ananya Kar,Aparna Sharma,Akhil Varshney,Vipin Kumar,Sandeep Saxena

View all publications

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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.

For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com

Anti-SLD5 antibody

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SLD5 antibody (AB101346)

Immunocytochemistry/ Immunofluorescence - Anti-SLD5 antibody (AB101346)

Western blot - Anti-SLD5 antibody (AB101346)

Western blot - Anti-SLD5 antibody (AB101346)

Key facts

Host species

Clonality

Isotype

Carrier free

Reacts with

Applications

Immunogen

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Reactivity data

Properties and storage information

Form

Purification technique

Storage buffer

Shipped at conditions

Appropriate short-term storage conditions

Appropriate long-term storage conditions

Aliquoting information

Storage information

Supplementary information

Biological function summary

Pathways

Product protocols

Target data

Publications (4)

Product promise