Rabbit Polyclonal SLD5 antibody. Suitable for WB, ICC/IF, IHC-P and reacts with Mouse, Human samples. Cited in 3 publications. Immunogen corresponding to Recombinant Fragment Protein within Human GINS4 aa 1-200.
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Publications
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- Frontiers in oncology 11:6541852021Overexpression of GINS4 Is Associated With Tumor Progression and Poor Survival in Hepatocellular Carcinoma.Applications:Unspecified applicationReactive species:Unspecified reactive speciesZiying Zhang,Peng Chen,Hui Xie,Peiguo CaoPubMed 33842367
- Journal of cancer research and clinical oncology 145:363-3712018Induced expression of GINS complex is an essential step for reactivation of quiescent stem-like tumor cells within the peri-necrotic niche in human glioblastoma.Applications:Unspecified applicationReactive species:Unspecified reactive speciesTokuhiro Kimura et. al.PubMed 30465075
- Molecular and cellular biology 38:2017Sld5 Ensures Centrosomal Resistance to Congression Forces by Preserving Centriolar Satellites.Applications:Unspecified applicationReactive species:Unspecified reactive speciesManpreet Kaur,Raksha Devi,Tanushree Ghosh,Md Muntaz Khan,Praveen Kumar, Priyanka,Ananya Kar,Aparna Sharma,Akhil Varshney,Vipin Kumar,Sandeep SaxenaPubMed 29061732
Key facts
- Isotype
- IgG
- Host species
- Rabbit
- Storage buffer
pH: 7
Preservative: 0.01% Thimerosal (merthiolate)
Constituents: 20% Glycerol (glycerin, glycerine), 1.21% Tris, 0.75% Glycine- Form
- Liquid
- Clonality
- Polyclonal
Immunogen
- Recombinant Fragment Protein within Human GINS4 aa 1-200. The exact immunogen used to generate this antibody is proprietary information. Database link Q9BRT9
Reactivity data
Select an application| WB | ICC/IF | IHC-P | |
|---|---|---|---|
| Human | Tested | Tested | Tested |
| Mouse | Tested | Expected | Expected |
WB
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Mouse | Dilution info 1/1000.00000 - 1/10000.00000 | Notes - |
Species Human | Dilution info 1/1000.00000 - 1/10000.00000 | Notes - |
ICC/IF
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info 1/100.00000 - 1/500.00000 | Notes - |
ExpectedExpected
| Species | Dilution info | Notes |
|---|---|---|
Species Mouse | Dilution info Use at an assay dependent concentration. | Notes - |
IHC-P
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info 1/100.00000 - 1/500.00000 | Notes Suggested antigen retrieval using heat mediated 10mM Citrate buffer (pH 6.0) or Tris-EDTA buffer (pH 8.0). |
ExpectedExpected
| Species | Dilution info | Notes |
|---|---|---|
Species Mouse | Dilution info Use at an assay dependent concentration. | Notes - |
Associated Products
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1 product for Alternative Product
Target data
Function
Required for correct functioning of the GINS complex, a complex that plays an essential role in the initiation of DNA replication, and progression of DNA replication forks (PubMed:17417653, PubMed:28414293). GINS complex is a core component of CDC45-MCM-GINS (CMG) helicase, the molecular machine that unwinds template DNA during replication, and around which the replisome is built (PubMed:32453425, PubMed:34694004, PubMed:34700328, PubMed:35585232).
Alternative names
SLD5, GINS4, DNA replication complex GINS protein SLD5, GINS complex subunit 4
Recommended products
Rabbit Polyclonal SLD5 antibody. Suitable for WB, ICC/IF, IHC-P and reacts with Mouse, Human samples. Cited in 3 publications. Immunogen corresponding to Recombinant Fragment Protein within Human GINS4 aa 1-200.
Key facts
- Isotype
- IgG
- Host species
- Rabbit
- Storage buffer
pH: 7
Preservative: 0.01% Thimerosal (merthiolate)
Constituents: 20% Glycerol (glycerin, glycerine), 1.21% Tris, 0.75% Glycine- Form
- Liquid
- Clonality
- Polyclonal
- Immunogen
- Recombinant Fragment Protein within Human GINS4 aa 1-200. The exact immunogen used to generate this antibody is proprietary information. Database link Q9BRT9
- Purification technique
- Affinity purification Immunogen
- Concentration
Storage
- Shipped at conditions
- Blue Ice
- Appropriate short-term storage conditions
- +4°C
- Appropriate long-term storage conditions
- -20°C
- Aliquoting information
- Upon delivery aliquot
- Storage information
- Avoid freeze / thaw cycle
Supplementary info
- This supplementary information is collated from multiple sources and compiled automatically.
- Activity summary
SLD5 also known as GINS4 is a subunit of the GINS complex. It has a molecular mass of approximately 22 kDa. SLD5 plays a significant role in DNA replication specifically during the initiation and elongation phases. The protein is expressed in various tissues with higher levels observed in proliferating cells. By binding to DNA replication origins SLD5 ensures the proper unwinding and loading of other replication factors.
- Biological function summary
SLD5 interacts with other components of the GINS complex to form a stable structure necessary for the replication fork's progression. The GINS complex comprising SLD5 PSF1 PSF2 and PSF3 couples with other protein assemblies like CDC45 and MCM (mini chromosome maintenance) proteins. This dynamic association enables the replication machinery to effectively duplicate the genome without errors.
- Pathways
SLD5 plays a part in the initiation of the DNA replication pathway. It partners with proteins like CDC45 and the MCM complex to form a replicative helicase. This assembly serves to unwound the DNA double helix facilitating replication. Furthermore interactions with ATR and ATM pathways position SLD5 within the larger network of DNA damage response ensuring any replication stress is adequately managed.
- Associated diseases and disorders
SLD5 exhibits connections with cancers such as breast cancer and colorectal cancer. Alterations in SLD5 expression can influence tumor progression by modulating cell proliferation rates. Through these pathways proteins like MCM and CDC45 are also implicated serving as key regulators in oncogenic processes where SLD5 activity expands its influence. Understanding these relationships offers insights into potential therapeutic targets for controlling abnormal cell growth.
Product promise
Tested
We have tested this species and application combination and it works. It is covered by our product promise.
Expected
We have not tested this specific species and application combination in-house, but expect it will work. It is covered by our product promise.
Predicted
This species and application combination has not been tested, but we predict it will work based on strong homology. However, this combination is not covered by our product promise.
Not recommended
We do not recommend this combination. It is not covered by our product promise.
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4 product images
Western blot - Anti-SLD5 antibody (ab101346)
12% SDS PAGE
All lanes: Western blot - Anti-SLD5 antibody (ab101346) at 1/1000 dilution
All lanes: 293T whole cell lysate at 30 µg
Predicted band size: 26 kDa
Western blot - Anti-SLD5 antibody (ab101346)
All lanes: Western blot - Anti-SLD5 antibody (ab101346) at 1/10000 dilution
All lanes: Mouse heart at 50 µg
Predicted band size: 26 kDa, 28 kDa, 59 kDa
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SLD5 antibody (ab101346)
ab101346 at 1/500 staining SLD5 in paraffin-embedded human gastric cancer.
Immunocytochemistry/ Immunofluorescence - Anti-SLD5 antibody (ab101346)
ab101346 at 1/200 staining paraformaldehyde-fixed A431 in IF. Bottom image costained with Hoechst 33342.
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