Rabbit Recombinant Monoclonal SLD5 antibody. Suitable for WB, Flow Cyt (Intra) and reacts with Human, Rat samples. Cited in 4 publications.
Images
![Flow Cytometry (Intracellular) - Anti-SLD5 antibody [EPR9318] (AB139683), expandable thumbnail](https://content.abcam.com/products/images/sld5-antibody-epr9318-ab139683--flow-cytometry-intracellular-img37800.jpg/_jcr_content/renditions/webp-475.webp "Flow Cytometry (Intracellular) - Anti-SLD5 antibody [EPR9318] (AB139683)")
![Western blot - Anti-SLD5 antibody [EPR9318] (AB139683), expandable thumbnail](https://content.abcam.com/products/images/sld5-antibody-epr9318-ab139683--western-blot-img101633.jpg/_jcr_content/renditions/webp-475.webp "Western blot - Anti-SLD5 antibody [EPR9318] (AB139683)")
Publications
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- OncoTargets and therapy 13:11433-114442020Expression Profile of GINS Complex Predicts the Prognosis of Pancreatic Cancer Patients.Applications:Unspecified applicationReactive species:Unspecified reactive speciesFanqin Bu et. al.PubMed 33192076
- PLoS biology 17:e20067672019The Cdk8/19-cyclin C transcription regulator functions in genome replication through metazoan Sld7.Applications:Unspecified applicationReactive species:Unspecified reactive speciesKerstin Köhler et. al.PubMed 30695077
- The Journal of clinical investigation 127:1991-20062017Inherited GINS1 deficiency underlies growth retardation along with neutropenia and NK cell deficiency.Applications:Unspecified applicationReactive species:Unspecified reactive speciesJulien Cottineau et. al.PubMed 28414293
- Nucleic acids research 45:169-1802016H3K9me3 demethylase Kdm4d facilitates the formation of pre-initiative complex and regulates DNA replication.Applications:WBReactive species:Unspecified reactive speciesRentian Wu,Zhiquan Wang,Honglian Zhang,Haiyun Gan,Zhiguo ZhangPubMed 27679476
Key facts
- Isotype
- IgG
- Host species
- Rabbit
- Storage buffer
pH: 7.2 - 7.4
Preservative: 0.01% Sodium azide
Constituents: 50% Tissue culture supernatant, 40% Glycerol (glycerin, glycerine), 9% PBS, 0.05% BSA- Form
- Liquid
- Clonality
- Monoclonal
Immunogen
- The exact immunogen used to generate this antibody is proprietary information.
Reactivity data
Select an application| ICC/IF | WB | IHC-P | Flow Cyt (Intra) | |
|---|---|---|---|---|
| Human | Not recommended | Tested | Not recommended | Tested |
| Mouse | Not recommended | Predicted | Not recommended | Predicted |
| Rat | Not recommended | Tested | Not recommended | Expected |
ICC/IF
Not recommendedNot recommended
| Species | Dilution info | Notes |
|---|---|---|
Species Rat, Human, Mouse | Dilution info - | Notes - |
WB
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info 1/1000 - 1/10000 | Notes - |
Species Rat | Dilution info 1/1000 - 1/10000 | Notes - |
PredictedPredicted
| Species | Dilution info | Notes |
|---|---|---|
Species Mouse | Dilution info - | Notes - |
IHC-P
Not recommendedNot recommended
| Species | Dilution info | Notes |
|---|---|---|
Species Rat, Human, Mouse | Dilution info - | Notes - |
Flow Cyt (Intra)
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info 1/100 - 1/500 | Notes ab172730 - Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody. |
ExpectedExpected
| Species | Dilution info | Notes |
|---|---|---|
Species Rat | Dilution info Use at an assay dependent concentration. | Notes - |
PredictedPredicted
| Species | Dilution info | Notes |
|---|---|---|
Species Mouse | Dilution info - | Notes - |
Associated Products
Select an associated product type
1 product for Alternative Product
Target data
Function
Required for correct functioning of the GINS complex, a complex that plays an essential role in the initiation of DNA replication, and progression of DNA replication forks (PubMed:17417653, PubMed:28414293). GINS complex is a core component of CDC45-MCM-GINS (CMG) helicase, the molecular machine that unwinds template DNA during replication, and around which the replisome is built (PubMed:32453425, PubMed:34694004, PubMed:34700328, PubMed:35585232).
Alternative names
SLD5, GINS4, DNA replication complex GINS protein SLD5, GINS complex subunit 4
Recommended products
Rabbit Recombinant Monoclonal SLD5 antibody. Suitable for WB, Flow Cyt (Intra) and reacts with Human, Rat samples. Cited in 4 publications.
Key facts
- Isotype
- IgG
- Host species
- Rabbit
- Storage buffer
pH: 7.2 - 7.4
Preservative: 0.01% Sodium azide
Constituents: 50% Tissue culture supernatant, 40% Glycerol (glycerin, glycerine), 9% PBS, 0.05% BSA- Form
- Liquid
- Clonality
- Monoclonal
- Immunogen
- The exact immunogen used to generate this antibody is proprietary information.
- Clone number
- EPR9318
- Purity
- Tissue culture supernatant
- Concentration
Storage
- Shipped at conditions
- Blue Ice
- Appropriate short-term storage conditions
- +4°C
- Appropriate long-term storage conditions
- -20°C
Notes
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free batch production
For more information, read more on recombinant antibodies.
Supplementary info
- This supplementary information is collated from multiple sources and compiled automatically.
- Activity summary
SLD5 also known as GINS4 is a subunit of the GINS complex. It has a molecular mass of approximately 22 kDa. SLD5 plays a significant role in DNA replication specifically during the initiation and elongation phases. The protein is expressed in various tissues with higher levels observed in proliferating cells. By binding to DNA replication origins SLD5 ensures the proper unwinding and loading of other replication factors.
- Biological function summary
SLD5 interacts with other components of the GINS complex to form a stable structure necessary for the replication fork's progression. The GINS complex comprising SLD5 PSF1 PSF2 and PSF3 couples with other protein assemblies like CDC45 and MCM (mini chromosome maintenance) proteins. This dynamic association enables the replication machinery to effectively duplicate the genome without errors.
- Pathways
SLD5 plays a part in the initiation of the DNA replication pathway. It partners with proteins like CDC45 and the MCM complex to form a replicative helicase. This assembly serves to unwound the DNA double helix facilitating replication. Furthermore interactions with ATR and ATM pathways position SLD5 within the larger network of DNA damage response ensuring any replication stress is adequately managed.
- Associated diseases and disorders
SLD5 exhibits connections with cancers such as breast cancer and colorectal cancer. Alterations in SLD5 expression can influence tumor progression by modulating cell proliferation rates. Through these pathways proteins like MCM and CDC45 are also implicated serving as key regulators in oncogenic processes where SLD5 activity expands its influence. Understanding these relationships offers insights into potential therapeutic targets for controlling abnormal cell growth.
Product promise
Tested
We have tested this species and application combination and it works. It is covered by our product promise.
Expected
We have not tested this specific species and application combination in-house, but expect it will work. It is covered by our product promise.
Predicted
This species and application combination has not been tested, but we predict it will work based on strong homology. However, this combination is not covered by our product promise.
Not recommended
We do not recommend this combination. It is not covered by our product promise.
We are dedicated to supporting your work with high quality reagents and we are here for you every step of the way should you need us.
In the unlikely event of one of our products not working as expected, you are covered by our product promise.
Full details and terms and conditions can be found here:
Terms & Conditions.
2 product images
![Flow Cytometry (Intracellular) - Anti-SLD5 antibody [EPR9318] (ab139683), expandable thumbnail](https://content.abcam.com/products/images/sld5-antibody-epr9318-ab139683--flow-cytometry-intracellular-img37800.jpg "Flow Cytometry (Intracellular) - Anti-SLD5 antibody [EPR9318] (ab139683)")
Flow Cytometry (Intracellular) - Anti-SLD5 antibody [EPR9318] (ab139683)
Intracellular flow cytometric analysis of permeabilized 293T cells labeling SLD5 with ab139683 at 1/100 dilution (red). Rabbit IgG negative control (green).
![Western blot - Anti-SLD5 antibody [EPR9318] (ab139683), expandable thumbnail](https://content.abcam.com/products/images/sld5-antibody-epr9318-ab139683--western-blot-img101633.jpg "Western blot - Anti-SLD5 antibody [EPR9318] (ab139683)")
Western blot - Anti-SLD5 antibody [EPR9318] (ab139683)
All lanes: Western blot - Anti-SLD5 antibody [EPR9318] (ab139683) at 1/1000 dilution
Lane 1: 293T cell lysate at 10 µg
Lane 2: PC3 cell lysate at 10 µg
Lane 3: Jurkat cell lysate at 10 µg
Lane 4: C6 cell lysate at 10 µg
SecondaryAll lanes: HRP labelled goat anti-rabbit at 1/2000 dilution
Predicted band size: 26 kDa
Downloads
Product protocols
- Visit the general protocols
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