AB139683

Anti-SLD5 antibody [EPR9318]

RabMAb
Recombinant
What is this?

Be the first to review this product! Submit a review

(5 Publications)

Rabbit Recombinant Monoclonal SLD5 antibody. Suitable for WB, Flow Cyt (Intra) and reacts with Human, Rat samples. Cited in 5 publications.

View Alternative Names

SLD5, GINS4, DNA replication complex GINS protein SLD5, GINS complex subunit 4

2 Images

Flow Cytometry (Intracellular) - Anti-SLD5 antibody [EPR9318] (AB139683)

Flow Cyt (Intra)

Unknown

Flow Cytometry (Intracellular) - Anti-SLD5 antibody [EPR9318] (AB139683)

Intracellular flow cytometric analysis of permeabilized 293T cells labeling SLD5 with ab139683 at 1/100 dilution (red). Rabbit IgG negative control (green).

Unknown

Western blot - Anti-SLD5 antibody [EPR9318] (AB139683)

All lanes:

Western blot - Anti-SLD5 antibody [EPR9318] (ab139683) at 1/1000 dilution

Lane 1:

293T cell lysate at 10 µg

Lane 2:

PC3 cell lysate at 10 µg

Lane 3:

Jurkat cell lysate at 10 µg

Lane 4:

C6 cell lysate at 10 µg

Secondary

All lanes:

HRP labelled goat anti-rabbit at 1/2000 dilution

Predicted band size: 26 kDa

false

Key facts

Host species

Rabbit

Clonality

Monoclonal

Clone number

EPR9318

Isotype

IgG

Carrier free

Reacts with

Rat, Human

Applications

Flow Cyt (Intra), WB

applications

Immunogen

The exact immunogen used to generate this antibody is proprietary information.

style

left-column

style

left-column

Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "ICCIF" : {"fullname" : "Immunocytochemistry/ Immunofluorescence", "shortname":"ICC/IF"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"}, "FlowCytIntra" : {"fullname" : "Flow Cytometry (Intracellular)", "shortname":"Flow Cyt (Intra)"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "ICCIF-species-checked": "notRecommended", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/1000 - 1/10000", "WB-species-notes": "", "IHCP-species-checked": "notRecommended", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "FlowCytIntra-species-checked": "testedAndGuaranteed", "FlowCytIntra-species-dilution-info": "1/100 - 1/500", "FlowCytIntra-species-notes": "<a href='/en-us/products/primary-antibodies/rabbit-igg-monoclonal-epr25a-isotype-control-ab172730'>ab172730</a> - Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody." }, "Mouse": { "ICCIF-species-checked": "notRecommended", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "", "IHCP-species-checked": "notRecommended", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "FlowCytIntra-species-checked": "predicted", "FlowCytIntra-species-dilution-info": "", "FlowCytIntra-species-notes": "" }, "Rat": { "ICCIF-species-checked": "notRecommended", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/1000 - 1/10000", "WB-species-notes": "", "IHCP-species-checked": "notRecommended", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "FlowCytIntra-species-checked": "guaranteed", "FlowCytIntra-species-dilution-info": "", "FlowCytIntra-species-notes": "" } } }

style

left-column

Product details

Patented technology
Our RabMAb^® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb^® patents.

What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:

- High batch-to-batch consistency and reproducibility
- Improved sensitivity and specificity
- Long-term security of supply
- Animal-free batch production

For more information, read more on recombinant antibodies.

style

left-column

Properties and storage information

Form

Liquid

Purity

Tissue culture supernatant

Storage buffer

pH: 7.2 - 7.4 Preservative: 0.01% Sodium azide Constituents: PBS, 50% Tissue culture supernatant, 40% Glycerol (glycerin, glycerine), 0.05% BSA

Shipped at conditions

Blue Ice

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

-20°C

style

left-column

Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

SLD5 also known as GINS4 is a subunit of the GINS complex. It has a molecular mass of approximately 22 kDa. SLD5 plays a significant role in DNA replication specifically during the initiation and elongation phases. The protein is expressed in various tissues with higher levels observed in proliferating cells. By binding to DNA replication origins SLD5 ensures the proper unwinding and loading of other replication factors.

Biological function summary

SLD5 interacts with other components of the GINS complex to form a stable structure necessary for the replication fork's progression. The GINS complex comprising SLD5 PSF1 PSF2 and PSF3 couples with other protein assemblies like CDC45 and MCM (mini chromosome maintenance) proteins. This dynamic association enables the replication machinery to effectively duplicate the genome without errors.

Pathways

SLD5 plays a part in the initiation of the DNA replication pathway. It partners with proteins like CDC45 and the MCM complex to form a replicative helicase. This assembly serves to unwound the DNA double helix facilitating replication. Furthermore interactions with ATR and ATM pathways position SLD5 within the larger network of DNA damage response ensuring any replication stress is adequately managed.

SLD5 exhibits connections with cancers such as breast cancer and colorectal cancer. Alterations in SLD5 expression can influence tumor progression by modulating cell proliferation rates. Through these pathways proteins like MCM and CDC45 are also implicated serving as key regulators in oncogenic processes where SLD5 activity expands its influence. Understanding these relationships offers insights into potential therapeutic targets for controlling abnormal cell growth.

style

left-column

style

left-column

Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

style

left-column

Target data

Required for correct functioning of the GINS complex, a complex that plays an essential role in the initiation of DNA replication, and progression of DNA replication forks (PubMed : 17417653, PubMed : 28414293). GINS complex is a core component of CDC45-MCM-GINS (CMG) helicase, the molecular machine that unwinds template DNA during replication, and around which the replisome is built (PubMed : 32453425, PubMed : 34694004, PubMed : 34700328, PubMed : 35585232).

See full target information GINS4

style

left-column

Publications (5)

Recent publications for all applications. Explore the full list and refine your search

Cells, tissues, organs 213:55-66 PubMed36273455

2022

MicroRNA-133a-3p Inhibits Lung Adenocarcinoma Development and Cisplatin Resistance through Targeting GINS4.

Applications

Unspecified application

Species

Unspecified reactive species

Yafu Zhou,Jianhua Yan,Huiguo Chen,Wenwu Zhou,Jinsong Yang

OncoTargets and therapy 13:11433-11444 PubMed33192076

2020

Expression Profile of GINS Complex Predicts the Prognosis of Pancreatic Cancer Patients.

Applications

Unspecified application

Species

Unspecified reactive species

Fanqin Bu,Xiaojian Zhu,Xuan Yi,Chen Luo,Kang Lin,Jinfeng Zhu,Cegui Hu,Zitao Liu,Jiefeng Zhao,Chao Huang,Wenjun Zhang,Jun Huang

PLoS biology 17:e2006767 PubMed30695077

2019

The Cdk8/19-cyclin C transcription regulator functions in genome replication through metazoan Sld7.

Applications

Unspecified application

Species

Unspecified reactive species

Kerstin Köhler,Luis Sanchez-Pulido,Verena Höfer,Anika Marko,Chris P Ponting,Ambrosius P Snijders,Regina Feederle,Aloys Schepers,Dominik Boos

The Journal of clinical investigation 127:1991-2006 PubMed28414293

2017

Inherited GINS1 deficiency underlies growth retardation along with neutropenia and NK cell deficiency.

Applications

Unspecified application

Species

Unspecified reactive species

Julien Cottineau,Molly C Kottemann,Francis P Lach,Young-Hoon Kang,Frédéric Vély,Elissa K Deenick,Tomi Lazarov,Laure Gineau,Yi Wang,Andrea Farina,Marie Chansel,Lazaro Lorenzo,Christelle Piperoglou,Cindy S Ma,Patrick Nitschke,Aziz Belkadi,Yuval Itan,Bertrand Boisson,Fabienne Jabot-Hanin,Capucine Picard,Jacinta Bustamante,Céline Eidenschenk,Soraya Boucherit,Nathalie Aladjidi,Didier Lacombe,Pascal Barat,Waseem Qasim,Jane A Hurst,Andrew J Pollard,Holm H Uhlig,Claire Fieschi,Jean Michon,Vladimir P Bermudez,Laurent Abel,Jean-Pierre de Villartay,Frédéric Geissmann,Stuart G Tangye,Jerard Hurwitz,Eric Vivier,Jean-Laurent Casanova,Agata Smogorzewska,Emmanuelle Jouanguy

Nucleic acids research 45:169-180 PubMed27679476

2016

H3K9me3 demethylase Kdm4d facilitates the formation of pre-initiative complex and regulates DNA replication.

Applications

Species

Unspecified reactive species

Rentian Wu,Zhiquan Wang,Honglian Zhang,Haiyun Gan,Zhiguo Zhang

View all publications

style

left-column

style

left-column

style

right-column

Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.

For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com

Anti-SLD5 antibody [EPR9318]

Flow Cytometry (Intracellular) - Anti-SLD5 antibody [EPR9318] (AB139683)

Western blot - Anti-SLD5 antibody [EPR9318] (AB139683)

Key facts

Host species

Clonality

Clone number

Isotype

Carrier free

Reacts with

Applications

Immunogen

Alternative Products

Primary Antibodies

Complementary Products

Primary Antibodies

Primary Antibodies

Primary Antibodies

Primary Antibodies

Reactivity data

Product details

Properties and storage information

Form

Purity

Storage buffer

Shipped at conditions

Appropriate short-term storage conditions

Appropriate long-term storage conditions

Supplementary information

Biological function summary

Pathways

Product protocols

Target data

Publications (5)

Product promise