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AB122480

Anti-SLF2 antibody

4

(1 Review)

|

(6 Publications)

Rabbit Polyclonal SLF2 antibody. Suitable for IHC-P, ICC/IF and reacts with Human samples. Cited in 6 publications. Immunogen corresponding to Recombinant Fragment Protein within Human SLF2 aa 300-400.

View Alternative Names

C10orf6, FAM178A, SLF2, SMC5-SMC6 complex localization factor protein 2, Smc5/6 localization factor 1

2 Images
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SLF2 antibody (AB122480)
  • IHC-P

Unknown

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SLF2 antibody (AB122480)

ab122480 at 1/500 dilution staining SLF2 in paraffin-embedded Human cerebellum tissue by Immunohistochemistry.

Immunocytochemistry/ Immunofluorescence - Anti-SLF2 antibody (AB122480)
  • ICC/IF

Unknown

Immunocytochemistry/ Immunofluorescence - Anti-SLF2 antibody (AB122480)

Immunofluorescent staining of Human cell line A-431 shows positivity in plasma membrane, cytoplasm and golgi apparatus. Recommended concentration of ab122480 1-4 µg/ml. Cells treated with PFA/Triton X-100.

Key facts

Host species

Rabbit

Clonality

Polyclonal

Isotype

IgG

Carrier free

No

Reacts with

Human

Applications

IHC-P, ICC/IF

applications

Immunogen

Recombinant Fragment Protein within Human SLF2 aa 300-400. The exact immunogen used to generate this antibody is proprietary information.

Q8IX21

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"}, "ICCIF" : {"fullname" : "Immunocytochemistry/ Immunofluorescence", "shortname":"ICC/IF"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "1/500 - 1/1000", "IHCP-species-notes": "<p></p> Perform heat-mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.", "ICCIF-species-checked": "testedAndGuaranteed", "ICCIF-species-dilution-info": "1-4 µg/mL", "ICCIF-species-notes": "<p>Recommend PFA Fixation and Triton X-100 treatment</p>" } } }
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Properties and storage information

Form
Liquid
Purification technique
Affinity purification Immunogen
Storage buffer
pH: 7.2 Preservative: 0.02% Sodium azide Constituents: PBS, 40% Glycerol (glycerin, glycerine)
Shipped at conditions
Blue Ice
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
-20°C
Aliquoting information
Upon delivery aliquot
Storage information
Avoid freeze / thaw cycle
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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

SLF2 also known as Smc5-Smc6 complex localization factor 2 functions mechanically as an important player in DNA repair processes. It weighs approximately 180 kDa and displays a ubiquitous expression pattern across various tissues in the body. SLF2 operates by facilitating the proper localization of the Smc5-Smc6 complex to sites of DNA double-strand breaks which is essential for maintaining genomic stability.
Biological function summary

The protein SLF2 contributes to the DNA damage response by interacting with other molecular components to modulate DNA repair pathways. It integrates into the Smc5-Smc6 complex which plays a role in homologous recombination repair. This interaction assists cells in efficiently repairing DNA damages and supports cell cycle progression further influencing cellular responses to DNA damage.

Pathways

SLF2 takes part in cellular mechanisms that control the DNA damage response and repair pathways. It directly associates with the DNA repair pathway involving major players like the ATM and ATR protein kinases which sense DNA damage and initiate repair signals. This connection highlights SLF2's involvement in the proper activation of downstream signaling cascades necessary for resolving DNA lesions effectively.

SLF2's role in maintaining genomic stability links it to certain conditions like cancer where DNA repair processes often malfunction. Deficiencies or mutations in SLF2 can disrupt its interaction with counterparts such as the Smc5-Smc6 complex contributing to chromosomal instability seen in cancerous cells. Additionally disorders like Fanconi anemia a genetic instability disorder may correspond with aberrant functioning of SLF2 and its complex interactions.
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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:
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Target data

Plays a role in the DNA damage response (DDR) pathway by regulating postreplication repair of UV-damaged DNA and genomic stability maintenance (PubMed : 25931565). The SLF1-SLF2 complex acts to link RAD18 with the SMC5-SMC6 complex at replication-coupled interstrand cross-links (ICL) and DNA double-strand breaks (DSBs) sites on chromatin during DNA repair in response to stalled replication forks (PubMed : 25931565). Promotes the recruitment of the SMC5-SMC6 complex to DNA lesions (PubMed : 25931565). Plays a role in SMC5-SMC6 complex recruitment for viral restriction. Forms a complex with SIMC1 and this complex is required to recruit SMC5-SMC6 complex to PML nuclear bodies and sites of viral replication (PubMed : 36373674).
See full target information SLF2
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Publications (6)

Recent publications for all applications. Explore the full list and refine your search

EMBO molecular medicine 15:e16431 PubMed37485814

2023

Actionable loss of SLF2 drives B-cell lymphomagenesis and impairs the DNA damage response.

Applications

Unspecified application

Species

Unspecified reactive species

Le Zhang,Matthias Wirth,Upayan Patra,Jacob Stroh,Konstandina Isaakidis,Leonie Rieger,Susanne Kossatz,Maja Milanovic,Chuanbing Zang,Uta Demel,Jan Keiten-Schmitz,Kristina Wagner,Katja Steiger,Roland Rad,Florian Bassermann,Stefan Müller,Ulrich Keller,Markus Schick

Journal of virology 97:e0032823 PubMed37338350

2023

SLF2 Interacts with the SMC5/6 Complex to Direct Hepatitis B Virus Episomal DNA to Promyelocytic Leukemia Bodies for Transcriptional Repression.

Applications

Unspecified application

Species

Unspecified reactive species

Qiyan Yao,Bo Peng,Cong Li,Xuelei Li,Mingyi Chen,Zhongmin Zhou,Dingbin Tang,Jiabei He,Yumeng Wu,Yinyan Sun,Wenhui Li

Nature microbiology 7:2101-2113 PubMed36376394

2022

Epigenetic silencing by the SMC5/6 complex mediates HIV-1 latency.

Applications

Unspecified application

Species

Unspecified reactive species

Ishak D Irwan,Hal P Bogerd,Bryan R Cullen

Nature structural & molecular biology 29:922-931 PubMed36097294

2022

Smc5/6 silences episomal transcription by a three-step function.

Applications

Unspecified application

Species

Unspecified reactive species

Fabien Abdul,Aurélie Diman,Bastien Baechler,Dhivya Ramakrishnan,Dmytro Kornyeyev,Rudolf K Beran,Simon P Fletcher,Michel Strubin

Cell host & microbe 29:792-805.e6 PubMed33811831

2021

The SMC5/6 complex compacts and silences unintegrated HIV-1 DNA and is antagonized by Vpr.

Applications

Unspecified application

Species

Unspecified reactive species

Liane Dupont,Stuart Bloor,James C Williamson,Sergio Martínez Cuesta,Raven Shah,Ana Teixeira-Silva,Adi Naamati,Edward J D Greenwood,Stefan G Sarafianos,Nicholas J Matheson,Paul J Lehner

Experimental and therapeutic medicine 11:421-426 PubMed26893625

2016

Increased expression of FERM domain-containing 4A protein is closely associated with the development of rectal cancer.

Applications

Unspecified application

Species

Unspecified reactive species

Yongtian Fan,Dechuan Li,Jun Qian,Yong Liu,Haiyang Feng,Dechuan Li
View all publications
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Product promise

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