Rabbit Polyclonal SLF2 antibody. Suitable for IHC-P, ICC/IF and reacts with Human samples. Cited in 1 publication. Immunogen corresponding to Recombinant Fragment Protein within Human SLF2 aa 300-400.
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- Cell host & microbe 29:792-805.e62021The SMC5/6 complex compacts and silences unintegrated HIV-1 DNA and is antagonized by Vpr.Applications:Unspecified applicationReactive species:Unspecified reactive speciesLiane Dupont,Stuart Bloor,James C Williamson,Sergio Martínez Cuesta,Raven Shah,Ana Teixeira-Silva,Adi Naamati,Edward J D Greenwood,Stefan G Sarafianos,Nicholas J Matheson,Paul J LehnerPubMed 33811831
Key facts
- Isotype
- IgG
- Host species
- Rabbit
- Storage buffer
pH: 7.2
Preservative: 0.02% Sodium azide
Constituents: 59% PBS, 40% Glycerol (glycerin, glycerine)- Form
- Liquid
- Clonality
- Polyclonal
Immunogen
- Recombinant Fragment Protein within Human SLF2 aa 300-400. The exact immunogen used to generate this antibody is proprietary information. Database link Q8IX21
Reactivity data
Select an application| IHC-P | ICC/IF | |
|---|---|---|
| Human | Tested | Tested |
IHC-P
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info 1/500.00000 - 1/1000.00000 | Notes Perform heat-mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol. |
ICC/IF
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info 1.00000-4.00000 µg/mL | Notes Recommend PFA Fixation and Triton X-100 treatment |
Target data
Function
Plays a role in the DNA damage response (DDR) pathway by regulating postreplication repair of UV-damaged DNA and genomic stability maintenance (PubMed:25931565). The SLF1-SLF2 complex acts to link RAD18 with the SMC5-SMC6 complex at replication-coupled interstrand cross-links (ICL) and DNA double-strand breaks (DSBs) sites on chromatin during DNA repair in response to stalled replication forks (PubMed:25931565). Promotes the recruitment of the SMC5-SMC6 complex to DNA lesions (PubMed:25931565). Plays a role in SMC5-SMC6 complex recruitment for viral restriction. Forms a complex with SIMC1 and this complex is required to recruit SMC5-SMC6 complex to PML nuclear bodies and sites of viral replication (PubMed:36373674).
Alternative names
C10orf6, FAM178A, SLF2, SMC5-SMC6 complex localization factor protein 2, Smc5/6 localization factor 1
Recommended products
Rabbit Polyclonal SLF2 antibody. Suitable for IHC-P, ICC/IF and reacts with Human samples. Cited in 1 publication. Immunogen corresponding to Recombinant Fragment Protein within Human SLF2 aa 300-400.
Key facts
- Isotype
- IgG
- Host species
- Rabbit
- Storage buffer
pH: 7.2
Preservative: 0.02% Sodium azide
Constituents: 59% PBS, 40% Glycerol (glycerin, glycerine)- Form
- Liquid
- Clonality
- Polyclonal
- Immunogen
- Recombinant Fragment Protein within Human SLF2 aa 300-400. The exact immunogen used to generate this antibody is proprietary information. Database link Q8IX21
- Purification technique
- Affinity purification Immunogen
- Concentration
Storage
- Shipped at conditions
- Blue Ice
- Appropriate short-term storage conditions
- +4°C
- Appropriate long-term storage conditions
- -20°C
- Aliquoting information
- Upon delivery aliquot
- Storage information
- Avoid freeze / thaw cycle
Notes
Abcam is leading the way to address reproducibility in scientific research with our highly validated recombinant monoclonal and recombinant multiclonal antibodies. Search & select one of Abcam's thousands of recombinant alternatives to eliminate batch-variability and unnecessary animal use.
If you do not find a host species to meet your needs, our catalogue and custom Chimeric range provides scientists the specificity of Abcam's RabMAbs in the species backbone of your choice. Remember to also review our range of edited cell lines, proteins and biochemicals relevant to your target that may help you further your research goals.
Abcam antibodies are extensively validated in a wide range of species and applications, so please check the reagent specifications meet your scientific needs before purchasing. If you have any questions or bespoke requirements, simply visit the Contact Us page to send us an inquiry or contact our Support Team ahead of purchase.
Supplementary info
- This supplementary information is collated from multiple sources and compiled automatically.
- Activity summary
SLF2 also known as Smc5-Smc6 complex localization factor 2 functions mechanically as an important player in DNA repair processes. It weighs approximately 180 kDa and displays a ubiquitous expression pattern across various tissues in the body. SLF2 operates by facilitating the proper localization of the Smc5-Smc6 complex to sites of DNA double-strand breaks which is essential for maintaining genomic stability.
- Biological function summary
The protein SLF2 contributes to the DNA damage response by interacting with other molecular components to modulate DNA repair pathways. It integrates into the Smc5-Smc6 complex which plays a role in homologous recombination repair. This interaction assists cells in efficiently repairing DNA damages and supports cell cycle progression further influencing cellular responses to DNA damage.
- Pathways
SLF2 takes part in cellular mechanisms that control the DNA damage response and repair pathways. It directly associates with the DNA repair pathway involving major players like the ATM and ATR protein kinases which sense DNA damage and initiate repair signals. This connection highlights SLF2's involvement in the proper activation of downstream signaling cascades necessary for resolving DNA lesions effectively.
- Associated diseases and disorders
SLF2's role in maintaining genomic stability links it to certain conditions like cancer where DNA repair processes often malfunction. Deficiencies or mutations in SLF2 can disrupt its interaction with counterparts such as the Smc5-Smc6 complex contributing to chromosomal instability seen in cancerous cells. Additionally disorders like Fanconi anemia a genetic instability disorder may correspond with aberrant functioning of SLF2 and its complex interactions.
Product promise
Tested
We have tested this species and application combination and it works. It is covered by our product promise.
Expected
We have not tested this specific species and application combination in-house, but expect it will work. It is covered by our product promise.
Predicted
This species and application combination has not been tested, but we predict it will work based on strong homology. However, this combination is not covered by our product promise.
Not recommended
We do not recommend this combination. It is not covered by our product promise.
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2 product images
Immunocytochemistry/ Immunofluorescence - Anti-SLF2 antibody (ab122480)
Immunofluorescent staining of Human cell line A-431 shows positivity in plasma membrane, cytoplasm and golgi apparatus. Recommended concentration of ab122480 1-4 µg/ml. Cells treated with PFA/Triton X-100.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SLF2 antibody (ab122480)
ab122480 at 1/500 dilution staining SLF2 in paraffin-embedded Human cerebellum tissue by Immunohistochemistry.
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