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AB256300

Anti-SLFN12 antibody [EPR20904-32] - BSA and Azide free

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(1 Publication)

Rabbit Recombinant Monoclonal SLFN12 antibody. Carrier free. Suitable for WB, IHC-Fr, Flow Cyt (Intra) and reacts with Human samples. Cited in 1 publication.

View Alternative Names

Ribonuclease SLFN12, Schlafen family member 12, SLFN12

2 Images
Immunohistochemistry (Frozen sections) - Anti-SLFN12 antibody [EPR20904-32] - BSA and Azide free (AB256300)
  • IHC-Fr

Unknown

Immunohistochemistry (Frozen sections) - Anti-SLFN12 antibody [EPR20904-32] - BSA and Azide free (AB256300)

Immunohistochemical analysis of 4% PFA-fixed, 0.2% Triton X-100 permeabilized frozen human placenta tissue labeling SLFN12 with ab234418 at 1/100 dilution (green), followed by Donkey anti-Rabbit IgG (H+L) secondary antibody, Alexa Fluor® 555 secondary at a 1/100 dilution. Positive staining in syncytiotrophoblast of human placenta is observed. Counterstained with DAPI (blue). Secondary antibody only control : Used PBS instead of primary antibody, secondary antibody is Donkey anti-Rabbit IgG (H+L) secondary antibody, Alexa Fluor® 555 used at a 1/100 dilution. Heat mediated antigen retrieval using sodium citrate buffer (10 mM citrate pH 6.0 and 0.05% Tween-20).

The image is kindly provided by Dr Xiaodong Wang.

This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab234418).

Flow Cytometry (Intracellular) - Anti-SLFN12 antibody [EPR20904-32] - BSA and Azide free (AB256300)
  • Flow Cyt (Intra)

Unknown

Flow Cytometry (Intracellular) - Anti-SLFN12 antibody [EPR20904-32] - BSA and Azide free (AB256300)

Intracellular flow cytometric analysis of 4% paraformaldehyde-fixed, 90% methanol permeabilized HeLa (human cervix adenocarcinoma epithelial cell) cell line labeling SLFN12 with ab234418 at 1/60 (red) compared with a Rabbit monoclonal IgG (ab172730) (black) and an unlabeled control (cells without incubation with primary antibody and secondary antibody) (blue). Goat anti rabbit IgG (Alexa Fluor® 488, ab150077), at 1/2000 dilution was used as the secondary antibody.

This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab234418).

  • Unconjugated

    Anti-SLFN12 antibody [EPR20904-32]

  • 617 Alexa Fluor® 594

    Alexa Fluor® 594 Anti-SLFN12 antibody [EPR20904-32]

  • 565 Alexa Fluor® 555

    Alexa Fluor® 555 Anti-SLFN12 antibody [EPR20904-32]

  • 519 Alexa Fluor® 488

    Alexa Fluor® 488 Anti-SLFN12 antibody [EPR20904-32]

  • 665 Alexa Fluor® 647

    Alexa Fluor® 647 Anti-SLFN12 antibody [EPR20904-32]

  • 660 APC

    APC Anti-SLFN12 antibody [EPR20904-32]

  • 578 PE

    PE Anti-SLFN12 antibody [EPR20904-32]

  • 775 Alexa Fluor® 750

    Alexa Fluor® 750 Anti-SLFN12 antibody [EPR20904-32]

Key facts

Host species

Rabbit

Clonality

Monoclonal

Clone number

EPR20904-32

Isotype

IgG

Carrier free

Yes

Reacts with

Human

Applications

Flow Cyt (Intra), IHC-Fr, WB

applications

Immunogen

The exact immunogen used to generate this antibody is proprietary information.

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "IP" : {"fullname" : "Immunoprecipitation", "shortname":"IP"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"}, "ICCIF" : {"fullname" : "Immunocytochemistry/ Immunofluorescence", "shortname":"ICC/IF"}, "IHCFr" : {"fullname" : "Immunohistochemistry (Frozen sections)", "shortname":"IHC-Fr"}, "FlowCytIntra" : {"fullname" : "Flow Cytometry (Intracellular)", "shortname":"Flow Cyt (Intra)"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "IP-species-checked": "notRecommended", "IP-species-dilution-info": "", "IP-species-notes": "<p></p>", "WB-species-checked": "guaranteed", "WB-species-dilution-info": "", "WB-species-notes": "<p></p>", "IHCP-species-checked": "notRecommended", "IHCP-species-dilution-info": "", "IHCP-species-notes": "<p></p>", "ICCIF-species-checked": "notRecommended", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "<p></p>", "IHCFr-species-checked": "testedAndGuaranteed", "IHCFr-species-dilution-info": "", "IHCFr-species-notes": "<p></p>", "FlowCytIntra-species-checked": "testedAndGuaranteed", "FlowCytIntra-species-dilution-info": "", "FlowCytIntra-species-notes": "<p></p>" } } }
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Product details

ab256300 is the carrier-free version of ab234418.

Patented technology
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:

  • - High batch-to-batch consistency and reproducibility
  • - Improved sensitivity and specificity
  • - Long-term security of supply
  • - Animal-free batch production

For more information, read more on recombinant antibodies.

Conjugation ready
Our carrier-free antibodies are typically supplied in a PBS-only formulation, purified and free of BSA, sodium azide and glycerol. This conjugation-ready format is designed for use with fluorochromes, metal isotopes, oligonucleotides, and enzymes, which makes them ideal for antibody labelling, functional and cell-based assays, flow-based assays (e.g. mass cytometry) and Multiplex Imaging applications.

Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with 1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.

Compatibility
This product is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm, without the need for antibody preparation. Maxpar® is a trademark of Fluidigm Canada Inc.

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Properties and storage information

Form
Liquid
Purification technique
Affinity purification Protein A
Storage buffer
pH: 7.2 - 7.4 Constituents: PBS
Shipped at conditions
Blue Ice
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
+4°C
Storage information
Do Not Freeze
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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

SLFN12 also known as Schlafen family member 12 is a protein that plays a role in protein synthesis regulation and innate immunity. It carries a mass of approximately 65 kDa. The protein shows expression in multiple human tissues including the colon small intestine and prostate. SLFN12 contains a notable AAA+ ATPase domain which suggests involvement in processes requiring energy such as molecular chaperone activity. Researchers have studied its expression pattern noting its presence at the cellular level which hints at diverse functional implications across various cells.
Biological function summary

SLFN12 modulates the differentiation of cells and appears to play a role in immune response. It does not form part of a large protein complex. It functions independently to exert effects specifically influencing maturation of specific immune cells and contributing to maintaining cellular homeostasis. Its role in differentiation makes it significant in balancing immune responses ensuring cells reach necessary maturity to perform their function in defense mechanisms.

Pathways

Studies highlight that SLFN12 contributes to the regulation of protein synthesis and the immune response pathway. In the synthesis pathway it potentially interacts with ribosomal proteins to regulate production of proteins modulating cell homoeostasis and growth. Meanwhile its involvement in the immune response pathway shows interaction with other SLFN family proteins altering cellular responses to microbial infections. Research also suggests it operates alongside proteins like SLFN11 to ensure effective cellular defense against pathogenic attacks.

Researchers have associated SLFN12 with inflammatory bowel disease and certain types of cancer such as prostate cancer. Its expression patterns in inflamed tissue suggest that SLFN12 may mediate inflammatory responses exacerbating or alleviating the condition. In prostate cancer SLFN12 seems to interact with the androgen receptor pathway potentially influencing cancer cell proliferation. Understanding these interactions and their pathological consequences will provide insights into its role in disease mechanisms and offer perspectives for therapeutics targeting SLFN12-mediated pathways.
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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:
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Target data

Ribonuclease which is part of an E2/17beta-estradiol-induced pro-apoptotic signaling pathway. E2 stabilizes the PDE3A/SLFN12 complex in the cytosol, promoting the dephosphorylation of SLFN12 and activating its pro-apoptotic ribosomal RNA/rRNA ribonuclease activity. This apoptotic pathway might be relevant in tissues with high concentration of E2 and be for instance involved in placenta remodeling (PubMed : 31420216, PubMed : 34272366, PubMed : 34707099, PubMed : 35104454). May play a role in cell differentiation (PubMed : 30045019).
See full target information SLFN12
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Publications (1)

Recent publications for all applications. Explore the full list and refine your search

Cell death & disease 15:573 PubMed39117605

2024

Elucidating the role of S100A10 in CD8 T cell exhaustion and HCC immune escape via the cPLA2 and 5-LOX axis.

Applications

Unspecified application

Species

Unspecified reactive species

Ganggang Wang,Xiaowei Shen,Wenzhi Jin,Chao Song,Meiyuan Dong,Zhijie Zhou,Xiaoliang Wang
View all publications
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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.
For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

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