Rabbit Polyclonal SLFN5 antibody. Suitable for IHC-P, WB, ICC/IF and reacts with Human samples. Cited in 5 publications. Immunogen corresponding to Synthetic Peptide within Human SLFN5 aa 50-250.
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Publications
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- iScience 26:1079682023Schlafen-5 inhibits LINE-1 retrotransposition.Applications:Unspecified applicationReactive species:Unspecified reactive speciesJiwei Ding,Shujie Wang,Qipeng Liu,Yuqing Duan,Tingting Cheng,Zhongjie Ye,Zhanding Cui,Ao Zhang,Qiuyu Liu,Zixiong Zhang,Ning Zhang,Qian Liu,Ni An,Jianyuan Zhao,Dongrong Yi,Quanjie Li,Jing Wang,Yongxin Zhang,Ling Ma,Saisai Guo,Jinhui Wang,Chen Liang,Jinming Zhou,Shan Cen,Xiaoyu LiPubMed 37810251
- Nucleic acids research 50:6137-61532022Schlafen 5 suppresses human immunodeficiency virus type 1 transcription by commandeering cellular epigenetic machinery.Applications:Unspecified applicationReactive species:Unspecified reactive speciesJiwei Ding,Shujie Wang,Zhen Wang,Shumin Chen,Jianyuan Zhao,Magan Solomon,Zhenlong Liu,Fei Guo,Ling Ma,Jiajia Wen,Xiaoyu Li,Chen Liang,Shan CenPubMed 35687115
- Cancer research 81:3664-36782021SLFN5 Regulates LAT1-Mediated mTOR Activation in Castration-Resistant Prostate Cancer.Applications:Unspecified applicationReactive species:Unspecified reactive speciesRafael S Martinez,Mark J Salji,Linda Rushworth,Chara Ntala,Giovanny Rodriguez Blanco,Ann Hedley,William Clark,Paul Peixoto,Eric Hervouet,Elodie Renaude,Sonia H Y Kung,Laura C A Galbraith,Colin Nixon,Sergio Lilla,Gillian M MacKay,Ladan Fazli,Luke Gaughan,David Sumpton,Martin E Gleave,Sara Zanivan,Arnaud Blomme,Hing Y LeungPubMed 33985973
- The Journal of clinical investigation 126:2867-802016Schlafen 4-expressing myeloid-derived suppressor cells are induced during murine gastric metaplasia.Applications:Unspecified applicationReactive species:Unspecified reactive speciesLin Ding et. al.PubMed 27427984
- Journal of gastroenterology 52:39-492016SCHLAFEN 5 expression correlates with intestinal metaplasia that progresses to gastric cancer.Applications:Unspecified applicationReactive species:Unspecified reactive speciesOsmel Companioni Nápoles,Amy C Tsao,José Miguel Sanz-Anquela,Núria Sala,Catalina Bonet,María Luisa Pardo,Lin Ding,Ornella Simo,Milena Saqui-Salces,Verónica Parra Blanco,Carlos A Gonzalez,Juanita L MerchantPubMed 27032393
Key facts
- Isotype
- IgG
- Host species
- Rabbit
- Storage buffer
pH: 7.2
Preservative: 0.02% Sodium azide
Constituents: 59% PBS, 40% Glycerol (glycerin, glycerine)- Form
- Liquid
- Clonality
- Polyclonal
Immunogen
- Synthetic Peptide within Human SLFN5 aa 50-250. The exact immunogen used to generate this antibody is proprietary information. Database link Q08AF3
Reactivity data
Select an application| IHC-P | WB | ICC/IF | |
|---|---|---|---|
| Human | Tested | Tested | Tested |
IHC-P
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info 1/500.00000 - 1/1000.00000 | Notes Perform heat-mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol. |
WB
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info 0.04000-0.40000 µg/mL | Notes - |
ICC/IF
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info 0.25000-2.00000 µg/mL | Notes - |
Target data
Function
May have a role in hematopoietic cell differentiation.
Alternative names
Schlafen family member 5, SLFN5
Recommended products
Rabbit Polyclonal SLFN5 antibody. Suitable for IHC-P, WB, ICC/IF and reacts with Human samples. Cited in 5 publications. Immunogen corresponding to Synthetic Peptide within Human SLFN5 aa 50-250.
Key facts
- Isotype
- IgG
- Host species
- Rabbit
- Storage buffer
pH: 7.2
Preservative: 0.02% Sodium azide
Constituents: 59% PBS, 40% Glycerol (glycerin, glycerine)- Form
- Liquid
- Clonality
- Polyclonal
- Immunogen
- Synthetic Peptide within Human SLFN5 aa 50-250. The exact immunogen used to generate this antibody is proprietary information. Database link Q08AF3
- Purification technique
- Affinity purification Immunogen
- Concentration
Storage
- Shipped at conditions
- Blue Ice
- Appropriate short-term storage conditions
- +4°C
- Appropriate long-term storage conditions
- -20°C
- Aliquoting information
- Upon delivery aliquot
- Storage information
- Avoid freeze / thaw cycle
Notes
Abcam is leading the way to address reproducibility in scientific research with our highly validated recombinant monoclonal and recombinant multiclonal antibodies. Search & select one of Abcam's thousands of recombinant alternatives to eliminate batch-variability and unnecessary animal use.
If you do not find a host species to meet your needs, our catalogue and custom Chimeric range provides scientists the specificity of Abcam's RabMAbs in the species backbone of your choice. Remember to also review our range of edited cell lines, proteins and biochemicals relevant to your target that may help you further your research goals.
Abcam antibodies are extensively validated in a wide range of species and applications, so please check the reagent specifications meet your scientific needs before purchasing. If you have any questions or bespoke requirements, simply visit the Contact Us page to send us an inquiry or contact our Support Team ahead of purchase.
Supplementary info
- This supplementary information is collated from multiple sources and compiled automatically.
- Activity summary
SLFN5 also known as Schlafen family member 5 is a protein with a mass of approximately 104 kDa. It functions as an ATP/GTP-binding protein which plays a role in RNA processing and decay. SLFN5 is localized predominantly in the cytoplasm and nucleus suggesting versatility in its functions. It is expressed in various tissues including immune and epithelial cells indicating its involvement in broad physiological processes.
- Biological function summary
SLFN5 plays a role in modulating immune cell responses and influencing cell proliferation. It acts as a regulatory element that affects the innate immune response possibly through interactions with nucleic acids. At a molecular level it associates with proteins involved in cell cycle regulation and differentiation therefore integrating into larger protein complexes that impact cell fate decisions.
- Pathways
SLFN5 is an integral part of the immune response and cell cycle regulation pathways. It interacts with other Schlafen family proteins as well as signal transducers like STAT1 to execute its regulatory functions. SLFN5's presence in these pathways indicates its role in maintaining cellular homeostasis and modulating responses to external stimuli such as viral infections.
- Associated diseases and disorders
SLFN5 associates with cancer and viral infections such as hepatitis C. It has shown expression changes in various cancer types including melanoma where it may influence tumor cell proliferation and immune evasion. SLFN5's interaction with proteins like STAT1 and other immune modulators suggests its involvement in the pathology and potentially highlights it as a target for therapeutic intervention.
Product promise
Tested
We have tested this species and application combination and it works. It is covered by our product promise.
Expected
We have not tested this specific species and application combination in-house, but expect it will work. It is covered by our product promise.
Predicted
This species and application combination has not been tested, but we predict it will work based on strong homology. However, this combination is not covered by our product promise.
Not recommended
We do not recommend this combination. It is not covered by our product promise.
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5 product images
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SLFN5 antibody (ab121537)
ab121537 at 1/725 dilution staining SLFN5 in paraffin-embedded Human fallopian tube by Immunohistochemistry.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SLFN5 antibody (ab121537)
ab121537 at 1/725 dilution staining SLFN5 in paraffin-embedded Human endometrium by Immunohistochemistry.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SLFN5 antibody (ab121537)
ab121537 at 1/725 dilution staining SLFN5 in paraffin-embedded Human lymph node by Immunohistochemistry.
Immunocytochemistry/ Immunofluorescence - Anti-SLFN5 antibody (ab121537)
Immunofluorescent staining of Human cell line A-431 shows positivity in nuclei but not nucleoli. Recommended concentration of ab121537 1-4 µg/ml. Cells treated with PFA/Triton X-100.
Western blot - Anti-SLFN5 antibody (ab121537)
Loading control: Anti-HDAC1
All lanes: Western blot - Anti-SLFN5 antibody (ab121537) at 0.4 µg/mL
Lane 1: SK-MEL-30 cell lysate
Lane 2: Caco-2 cell lysate
Predicted band size: 101 kDa
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