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AB121537

Anti-SLFN5 antibody

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(5 Publications)

Rabbit Polyclonal SLFN5 antibody. Suitable for IHC-P, WB, ICC/IF and reacts with Human samples. Cited in 5 publications. Immunogen corresponding to Synthetic Peptide within Human SLFN5 aa 50-250.

View Alternative Names

Schlafen family member 5, SLFN5

5 Images
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SLFN5 antibody (AB121537)
  • IHC-P

Unknown

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SLFN5 antibody (AB121537)

ab121537 at 1/725 dilution staining SLFN5 in paraffin-embedded Human lymph node by Immunohistochemistry.

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SLFN5 antibody (AB121537)
  • IHC-P

Unknown

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SLFN5 antibody (AB121537)

ab121537 at 1/725 dilution staining SLFN5 in paraffin-embedded Human endometrium by Immunohistochemistry.

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SLFN5 antibody (AB121537)
  • IHC-P

Unknown

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SLFN5 antibody (AB121537)

ab121537 at 1/725 dilution staining SLFN5 in paraffin-embedded Human fallopian tube by Immunohistochemistry.

Immunocytochemistry/ Immunofluorescence - Anti-SLFN5 antibody (AB121537)
  • ICC/IF

Unknown

Immunocytochemistry/ Immunofluorescence - Anti-SLFN5 antibody (AB121537)

Immunofluorescent staining of Human cell line A-431 shows positivity in nuclei but not nucleoli. Recommended concentration of ab121537 1-4 µg/ml. Cells treated with PFA/Triton X-100.

Western blot - Anti-SLFN5 antibody (AB121537)
  • WB

Supplier Data

Western blot - Anti-SLFN5 antibody (AB121537)

Loading control : Anti-HDAC1

All lanes:

Western blot - Anti-SLFN5 antibody (ab121537) at 0.4 µg/mL

Lane 1:

SK-MEL-30 cell lysate

Lane 2:

Caco-2 cell lysate

Predicted band size: 101 kDa

false

Key facts

Host species

Rabbit

Clonality

Polyclonal

Isotype

IgG

Carrier free

No

Reacts with

Human

Applications

ICC/IF, WB, IHC-P

applications

Immunogen

Synthetic Peptide within Human SLFN5 aa 50-250. The exact immunogen used to generate this antibody is proprietary information.

Q08AF3

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "ICCIF" : {"fullname" : "Immunocytochemistry/ Immunofluorescence", "shortname":"ICC/IF"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "1/500 - 1/1000", "IHCP-species-notes": "<p></p> Perform heat-mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "0.04-0.4 µg/mL", "WB-species-notes": "<p></p>", "ICCIF-species-checked": "testedAndGuaranteed", "ICCIF-species-dilution-info": "0.25-2 µg/mL", "ICCIF-species-notes": "<p></p>" } } }
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Properties and storage information

Form
Liquid
Purification technique
Affinity purification Immunogen
Storage buffer
pH: 7.2 Preservative: 0.02% Sodium azide Constituents: PBS, 40% Glycerol (glycerin, glycerine)
Shipped at conditions
Blue Ice
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
-20°C
Aliquoting information
Upon delivery aliquot
Storage information
Avoid freeze / thaw cycle
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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

SLFN5 also known as Schlafen family member 5 is a protein with a mass of approximately 104 kDa. It functions as an ATP/GTP-binding protein which plays a role in RNA processing and decay. SLFN5 is localized predominantly in the cytoplasm and nucleus suggesting versatility in its functions. It is expressed in various tissues including immune and epithelial cells indicating its involvement in broad physiological processes.
Biological function summary

SLFN5 plays a role in modulating immune cell responses and influencing cell proliferation. It acts as a regulatory element that affects the innate immune response possibly through interactions with nucleic acids. At a molecular level it associates with proteins involved in cell cycle regulation and differentiation therefore integrating into larger protein complexes that impact cell fate decisions.

Pathways

SLFN5 is an integral part of the immune response and cell cycle regulation pathways. It interacts with other Schlafen family proteins as well as signal transducers like STAT1 to execute its regulatory functions. SLFN5's presence in these pathways indicates its role in maintaining cellular homeostasis and modulating responses to external stimuli such as viral infections.

SLFN5 associates with cancer and viral infections such as hepatitis C. It has shown expression changes in various cancer types including melanoma where it may influence tumor cell proliferation and immune evasion. SLFN5's interaction with proteins like STAT1 and other immune modulators suggests its involvement in the pathology and potentially highlights it as a target for therapeutic intervention.
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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:
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Target data

May have a role in hematopoietic cell differentiation.
See full target information SLFN5
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Publications (5)

Recent publications for all applications. Explore the full list and refine your search

iScience 26:107968 PubMed37810251

2023

Schlafen-5 inhibits LINE-1 retrotransposition.

Applications

Unspecified application

Species

Unspecified reactive species

Jiwei Ding,Shujie Wang,Qipeng Liu,Yuqing Duan,Tingting Cheng,Zhongjie Ye,Zhanding Cui,Ao Zhang,Qiuyu Liu,Zixiong Zhang,Ning Zhang,Qian Liu,Ni An,Jianyuan Zhao,Dongrong Yi,Quanjie Li,Jing Wang,Yongxin Zhang,Ling Ma,Saisai Guo,Jinhui Wang,Chen Liang,Jinming Zhou,Shan Cen,Xiaoyu Li

Nucleic acids research 50:6137-6153 PubMed35687115

2022

Schlafen 5 suppresses human immunodeficiency virus type 1 transcription by commandeering cellular epigenetic machinery.

Applications

Unspecified application

Species

Unspecified reactive species

Jiwei Ding,Shujie Wang,Zhen Wang,Shumin Chen,Jianyuan Zhao,Magan Solomon,Zhenlong Liu,Fei Guo,Ling Ma,Jiajia Wen,Xiaoyu Li,Chen Liang,Shan Cen

Cancer research 81:3664-3678 PubMed33985973

2021

SLFN5 Regulates LAT1-Mediated mTOR Activation in Castration-Resistant Prostate Cancer.

Applications

Unspecified application

Species

Unspecified reactive species

Rafael S Martinez,Mark J Salji,Linda Rushworth,Chara Ntala,Giovanny Rodriguez Blanco,Ann Hedley,William Clark,Paul Peixoto,Eric Hervouet,Elodie Renaude,Sonia H Y Kung,Laura C A Galbraith,Colin Nixon,Sergio Lilla,Gillian M MacKay,Ladan Fazli,Luke Gaughan,David Sumpton,Martin E Gleave,Sara Zanivan,Arnaud Blomme,Hing Y Leung

The Journal of clinical investigation 126:2867-80 PubMed27427984

2016

Schlafen 4-expressing myeloid-derived suppressor cells are induced during murine gastric metaplasia.

Applications

Unspecified application

Species

Unspecified reactive species

Lin Ding,Michael M Hayes,Amanda Photenhauer,Kathryn A Eaton,Qian Li,Ramon Ocadiz-Ruiz,Juanita L Merchant

Journal of gastroenterology 52:39-49 PubMed27032393

2016

SCHLAFEN 5 expression correlates with intestinal metaplasia that progresses to gastric cancer.

Applications

Unspecified application

Species

Unspecified reactive species

Osmel Companioni Nápoles,Amy C Tsao,José Miguel Sanz-Anquela,Núria Sala,Catalina Bonet,María Luisa Pardo,Lin Ding,Ornella Simo,Milena Saqui-Salces,Verónica Parra Blanco,Carlos A Gonzalez,Juanita L Merchant
View all publications
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Product promise

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