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AB204945

Anti-Slingshot homolog 1/SSH1 antibody [6C11G9]

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(1 Publication)

Mouse Monoclonal Slingshot homolog 1/SSH1 antibody. Suitable for Flow Cyt, IHC-P and reacts with Human samples. Cited in 1 publication. Immunogen corresponding to Synthetic Peptide within Human SSH1 aa 1000 to C-terminus.

View Alternative Names

KIAA1298, SSH1L, SSH1, Protein phosphatase Slingshot homolog 1, SSH-like protein 1, SSH-1L, hSSH-1L

2 Images
Flow Cytometry - Anti-Slingshot homolog 1/SSH1 antibody [6C11G9] (AB204945)
  • Flow Cyt

Supplier Data

Flow Cytometry - Anti-Slingshot homolog 1/SSH1 antibody [6C11G9] (AB204945)

Flow cytometric analysis of Hela cells labeling Slingshot homolog 1/SSH1 using ab204945 at 1/200 (green) and negative control (red).

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Slingshot homolog 1/SSH1 antibody [6C11G9] (AB204945)
  • IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Slingshot homolog 1/SSH1 antibody [6C11G9] (AB204945)

Immunohistochemical analysis of paraffin-embedded Human ovarian cancer tissue labeling Slingshot homolog 1/SSH1 with ab204945 at 1/200 with DAB staining.

Key facts

Host species

Mouse

Clonality

Monoclonal

Clone number

6C11G9

Isotype

IgG1

Carrier free

No

Reacts with

Human

Applications

Flow Cyt, IHC-P

applications

Immunogen

Synthetic Peptide within Human SSH1 aa 1000 to C-terminus. The exact immunogen used to generate this antibody is proprietary information.

Q8WYL5

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "FlowCyt" : {"fullname" : "Flow Cytometry", "shortname":"Flow Cyt"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "FlowCyt-species-checked": "testedAndGuaranteed", "FlowCyt-species-dilution-info": "1/200 - 1/400", "FlowCyt-species-notes": "<p></p>", "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "1/200 - 1/1000", "IHCP-species-notes": "<p></p>" } } }
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Properties and storage information

Form
Liquid
Purification technique
Affinity purification Protein G
Purification notes
Purified from tissue culture supernatant.
Storage buffer
Preservative: 0.05% Sodium azide Constituents: PBS
Shipped at conditions
Blue Ice
Appropriate short-term storage duration
1-2 weeks
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
-20°C
Aliquoting information
Upon delivery aliquot
Storage information
Avoid freeze / thaw cycle
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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

Slingshot homolog 1 (SSH1) also known as SSH1L is a protein phosphatase with a molecular mass of approximately 133 kDa. SSH1 is expressed in many tissues with higher levels in the brain and lung. It specifically dephosphorylates and activates cofilin a protein involved in actin dynamics. SSH1 contains a phosphatase domain essential for its activity which targets serine/threonine residues on substrates.
Biological function summary

SSH1 plays a critical role in the regulation of actin filament dynamics. It is not part of a known complex but interacts with several proteins involved in cytoskeleton organization. SSH1 reactivates cofilin by dephosphorylation facilitating the disassembly of actin filaments which is vital for processes such as cell migration division and morphogenesis. The ability of SSH1 to regulate actin dynamics makes it important for maintaining cell shape and motility.

Pathways

SSH1 contributes significantly to the actin dynamics pathway influencing cell architecture and movement. It operates downstream of the Rho family of GTPases which modulate actin reorganization. SSH1 also interacts with LIM kinase (LIMK) through the cofilin pathway. LIMK phosphorylates and inactivates cofilin while SSH1 reverses this action establishing a feedback loop critical for actin turnover.

SSH1 has implications in cancer and neurodegenerative diseases. In cancer abnormalities in SSH1 function can lead to enhanced cell migration and metastasis partly through interactions with cofilin and other cytoskeletal regulators. In neurodegenerative diseases altered SSH1 activity affects neuronal cell shape and connectivity. Dysregulation of cofilin by SSH1 is linked to the progression of these conditions highlighting the potential targeting of SSH1-related pathways for therapeutic interventions.
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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:
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Target data

Protein phosphatase which regulates actin filament dynamics. Dephosphorylates and activates the actin binding/depolymerizing factor cofilin, which subsequently binds to actin filaments and stimulates their disassembly. Inhibitory phosphorylation of cofilin is mediated by LIMK1, which may also be dephosphorylated and inactivated by this protein.
See full target information SSH1
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Publications (1)

Recent publications for all applications. Explore the full list and refine your search

Environmental toxicology 36:2322-2332 PubMed34418280

2021

Long noncoding RNA FAM66C promotes tumor progression and glycolysis in intrahepatic cholangiocarcinoma by regulating hsa-miR-23b-3p/KCND2 axis.

Applications

Unspecified application

Species

Unspecified reactive species

Guang-Lin Lei,Zhi Li,Yuan-Yuan Li,Zhi-Xian Hong,Sen Wang,Zhi-Fang Bai,Fang Sun,Jin Yan,Ling-Xiang Yu,Peng-Hui Yang,Zhan-Yu Yang
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Product promise

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