AB264195

Anti-Slingshot homolog 1/SSH1 antibody

Name: Anti-Slingshot homolog 1/SSH1 antibody (ab264195) | Abcam
Brand: Abcam Limited
SKU: AB264195
Price: 525 USD
Availability: InStock

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Rabbit Polyclonal Slingshot homolog 1/SSH1 antibody. Suitable for IP, WB and reacts with Human samples. Immunogen corresponding to Synthetic Peptide within Human SSH1 aa 950 to C-terminus.

View Alternative Names

KIAA1298, SSH1L, SSH1, Protein phosphatase Slingshot homolog 1, SSH-like protein 1, SSH-1L, hSSH-1L

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Supplier Data

Immunoprecipitation - Anti-Slingshot homolog 1/SSH1 antibody (AB264195)

Slingshot homolog 1/SSH1 was immunoprecipitated from 1mg HeLa whole cell lysate using ab264195 at 6 μg per reaction. Western blot was performed on the immunoprecipitate using ab264195 at 1 μg/ml.
Lane 1 : ab264195 IP in HeLa whole cell lysate.
Lane 2 : Control IgG.

Cells prepared using NETN lysis buffer.

All lanes:

Immunoprecipitation - Anti-Slingshot homolog 1/SSH1 antibody (ab264195)

Predicted band size: 116 kDa

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Exposure time: 3min

Supplier Data

Western blot - Anti-Slingshot homolog 1/SSH1 antibody (AB264195)

Cells prepared using NETN lysis buffer.

Secondary : Goat anti-Rabbit Light Chain HRP Conjugate.

4-8% SDS-PAGE.

All lanes:

Western blot - Anti-Slingshot homolog 1/SSH1 antibody (ab264195) at 0.1 µg/mL

Lane 1:

HeLa whole cell lysate at 50 µg

Lane 2:

HeLa whole cell lysate at 15 µg

Lane 3:

HEK-293T whole cell lysate at 50 µg

Predicted band size: 116 kDa

true

Exposure time: 3min

Key facts

Host species

Rabbit

Clonality

Polyclonal

Isotype

IgG

Carrier free

Reacts with

Human

Applications

WB, IP

applications

Immunogen

Synthetic Peptide within Human SSH1 aa 950 to C-terminus. The exact immunogen used to generate this antibody is proprietary information.

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "IP" : {"fullname" : "Immunoprecipitation", "shortname":"IP"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "IP-species-checked": "testedAndGuaranteed", "IP-species-dilution-info": "2-10 µg/mg of lysate", "IP-species-notes": "<p></p>", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/2000 - 1/10000", "WB-species-notes": "<p></p>" } } }

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Properties and storage information

Form

Liquid

Purification technique

Affinity purification Immunogen

Storage buffer

pH: 6.8 - 7.4 Preservative: 0.09% Sodium azide Constituents: Tris buffered saline, 0.1% BSA

Shipped at conditions

Blue Ice

Appropriate short-term storage duration

1-2 weeks

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

-20°C

Aliquoting information

Upon delivery aliquot

Storage information

Avoid freeze / thaw cycle

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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

Slingshot homolog 1 (SSH1) also known as SSH1L is a protein phosphatase with a molecular mass of approximately 133 kDa. SSH1 is expressed in many tissues with higher levels in the brain and lung. It specifically dephosphorylates and activates cofilin a protein involved in actin dynamics. SSH1 contains a phosphatase domain essential for its activity which targets serine/threonine residues on substrates.

Biological function summary

SSH1 plays a critical role in the regulation of actin filament dynamics. It is not part of a known complex but interacts with several proteins involved in cytoskeleton organization. SSH1 reactivates cofilin by dephosphorylation facilitating the disassembly of actin filaments which is vital for processes such as cell migration division and morphogenesis. The ability of SSH1 to regulate actin dynamics makes it important for maintaining cell shape and motility.

Pathways

SSH1 contributes significantly to the actin dynamics pathway influencing cell architecture and movement. It operates downstream of the Rho family of GTPases which modulate actin reorganization. SSH1 also interacts with LIM kinase (LIMK) through the cofilin pathway. LIMK phosphorylates and inactivates cofilin while SSH1 reverses this action establishing a feedback loop critical for actin turnover.

SSH1 has implications in cancer and neurodegenerative diseases. In cancer abnormalities in SSH1 function can lead to enhanced cell migration and metastasis partly through interactions with cofilin and other cytoskeletal regulators. In neurodegenerative diseases altered SSH1 activity affects neuronal cell shape and connectivity. Dysregulation of cofilin by SSH1 is linked to the progression of these conditions highlighting the potential targeting of SSH1-related pathways for therapeutic interventions.

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

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Target data

Protein phosphatase which regulates actin filament dynamics. Dephosphorylates and activates the actin binding/depolymerizing factor cofilin, which subsequently binds to actin filaments and stimulates their disassembly. Inhibitory phosphorylation of cofilin is mediated by LIMK1, which may also be dephosphorylated and inactivated by this protein.

See full target information SSH1

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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.

For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

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Anti-Slingshot homolog 1/SSH1 antibody

Immunoprecipitation - Anti-Slingshot homolog 1/SSH1 antibody (AB264195)

Western blot - Anti-Slingshot homolog 1/SSH1 antibody (AB264195)

Key facts

Host species

Clonality

Isotype

Carrier free

Reacts with

Applications

Immunogen

Reactivity data

Properties and storage information

Form

Purification technique

Storage buffer

Shipped at conditions

Appropriate short-term storage duration

Appropriate short-term storage conditions

Appropriate long-term storage conditions

Aliquoting information

Storage information

Supplementary information

Biological function summary

Pathways

Product protocols

Target data

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