AB76943

Anti-Slingshot homolog 1/SSH1 antibody

Name: Anti-Slingshot homolog 1/SSH1 antibody (ab76943) | Abcam
Brand: Abcam Limited
SKU: AB76943

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(12 Publications)

Rabbit Polyclonal Slingshot homolog 1/SSH1 antibody. Suitable for IP, WB and reacts with Human samples. Cited in 12 publications. Immunogen corresponding to Synthetic Peptide within Human SSH1 aa 650-750.

View Alternative Names

KIAA1298, SSH1L, SSH1, Protein phosphatase Slingshot homolog 1, SSH-like protein 1, SSH-1L, hSSH-1L

2 Images

Supplier Data

Immunoprecipitation - Anti-Slingshot homolog 1/SSH1 antibody (AB76943)

Slingshot/ SSH1 was immunoprecipitated from HeLa whole cell lysate (1 mg per IP reaction, 20% loaded) with ab76943 at 3 μg per reaction. Western blot was performed on the immunoprecipitate using ab76943 at 0.04 μg/mL.

Lane 1 : ab76943 IP in HeLa whole cell lysate.

Lane 2 : rabbit anti-SSH1 antibody recognising a downstream epitope IP in HeLa whole cell lysate.

Lane 3 : rabbit anti-SSH1 antibody recognising a downstream epitope IP in HeLa whole cell lysate.

Lane 4 : Control IgG in HeLa whole cell lysate.

Detection : Chemiluminescence with an exposure time of 10 seconds

All lanes:

Immunoprecipitation - Anti-Slingshot homolog 1/SSH1 antibody (ab76943)

Predicted band size: 116 kDa

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Western blot - Anti-Slingshot homolog 1/SSH1 antibody (AB76943)

All lanes:

Western blot - Anti-Slingshot homolog 1/SSH1 antibody (ab76943) at 0.04 µg/mL

Lane 1:

HeLa whole cell lysate at 50 µg

Lane 2:

HeLa whole cell lysate at 15 µg

Lane 3:

HeLa whole cell lysate at 5 µg

Lane 4:

293T whole cell lysate at 50 µg

Predicted band size: 116 kDa

Observed band size: 150 kDa

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Key facts

Host species

Rabbit

Clonality

Polyclonal

Isotype

IgG

Carrier free

Reacts with

Human

Applications

IP, WB

applications

Immunogen

Synthetic Peptide within Human SSH1 aa 650-750. The exact immunogen used to generate this antibody is proprietary information.

Q8WYL5

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "IP" : {"fullname" : "Immunoprecipitation", "shortname":"IP"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "IP-species-checked": "testedAndGuaranteed", "IP-species-dilution-info": "2-5 µg/mg of lysate", "IP-species-notes": "<p></p>", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/2000 - 1/10000", "WB-species-notes": "<p></p>" }, "Gorilla": { "IP-species-checked": "predicted", "IP-species-dilution-info": "", "IP-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "" }, "Orangutan": { "IP-species-checked": "predicted", "IP-species-dilution-info": "", "IP-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "" } } }

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Properties and storage information

Form

Liquid

Purification technique

Affinity purification Immunogen

Purification notes

ab76943 was affinity purified using an epitope specific to Slingshot homolog 1/SSH1 immobilized on solid support.

Storage buffer

pH: 6.8 - 7.4 Preservative: 0.09% Sodium azide Constituents: Tris buffered saline, 0.1% BSA

Shipped at conditions

Blue Ice

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

+4°C

Aliquoting information

Upon delivery aliquot

Storage information

Avoid freeze / thaw cycle

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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

Slingshot homolog 1 (SSH1) also known as SSH1L is a protein phosphatase with a molecular mass of approximately 133 kDa. SSH1 is expressed in many tissues with higher levels in the brain and lung. It specifically dephosphorylates and activates cofilin a protein involved in actin dynamics. SSH1 contains a phosphatase domain essential for its activity which targets serine/threonine residues on substrates.

Biological function summary

SSH1 plays a critical role in the regulation of actin filament dynamics. It is not part of a known complex but interacts with several proteins involved in cytoskeleton organization. SSH1 reactivates cofilin by dephosphorylation facilitating the disassembly of actin filaments which is vital for processes such as cell migration division and morphogenesis. The ability of SSH1 to regulate actin dynamics makes it important for maintaining cell shape and motility.

Pathways

SSH1 contributes significantly to the actin dynamics pathway influencing cell architecture and movement. It operates downstream of the Rho family of GTPases which modulate actin reorganization. SSH1 also interacts with LIM kinase (LIMK) through the cofilin pathway. LIMK phosphorylates and inactivates cofilin while SSH1 reverses this action establishing a feedback loop critical for actin turnover.

SSH1 has implications in cancer and neurodegenerative diseases. In cancer abnormalities in SSH1 function can lead to enhanced cell migration and metastasis partly through interactions with cofilin and other cytoskeletal regulators. In neurodegenerative diseases altered SSH1 activity affects neuronal cell shape and connectivity. Dysregulation of cofilin by SSH1 is linked to the progression of these conditions highlighting the potential targeting of SSH1-related pathways for therapeutic interventions.

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:

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Target data

Protein phosphatase which regulates actin filament dynamics. Dephosphorylates and activates the actin binding/depolymerizing factor cofilin, which subsequently binds to actin filaments and stimulates their disassembly. Inhibitory phosphorylation of cofilin is mediated by LIMK1, which may also be dephosphorylated and inactivated by this protein.

See full target information SSH1

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Publications (12)

Recent publications for all applications. Explore the full list and refine your search

Frontiers in medicine 9:861371 PubMed35492354

2022

Chaperonin-Containing TCP1 Subunit 5 Protects Against the Effect of Mer Receptor Tyrosine Kinase Knockdown in Retinal Pigment Epithelial Cells by Interacting With Filamentous Actin and Activating the LIM-Kinase 1/Cofilin Pathway.

Applications

Unspecified application

Species

Unspecified reactive species

Lujia Feng,Haichun Li,Yong Du,Ting Zhang,Yingting Zhu,Zhidong Li,Ling Zhao,Xing Wang,Gongpei Wang,Linbin Zhou,Zhaorong Jiang,Zheng Liu,Zhancong Ou,Yuwen Wen,Yehong Zhuo

Cell & bioscience 10:113 PubMed32983407

2020

Vascular NRP2 triggers PNET angiogenesis by activating the SSH1-cofilin axis.

Applications

Unspecified application

Species

Unspecified reactive species

Xi Luo,Jiang-Yi He,Jie Xu,Shao-Yi Hu,Bang-Hui Mo,Qiu-Xia Shu,Can Chen,Yu-Zhu Gong,Xiao-Long Zhao,Gan-Feng Xie,Song-Tao Yu

World journal of urology 38:2849-2856 PubMed31965287

2020

Slingshot homolog-1 expression is a poor prognostic factor of pT1 bladder urothelial carcinoma after transurethral resection.

Applications

Unspecified application

Species

Unspecified reactive species

Qiang Luo,Yanxia Liu,Hu Zhao,Peng Guo,Qianwen Wang,Wenjun Li,Gang Li,Bin Wu

BMC gastroenterology 18:12 PubMed29338701

2018

SSH1 expression is associated with gastric cancer progression and predicts a poor prognosis.

Applications

Unspecified application

Species

Unspecified reactive species

Yusufu Maimaiti,Maimaitiaili Maimaitiming,Yiliang Li,Saifuding Aibibula,Azatijiang Ainiwaer,Aikebaier Aili,Zhenzhu Sun,Kelimu Abudureyimu

Oncotarget 8:66195-66203 PubMed29029503

2017

Slingshot-1L, a cofilin phosphatase, induces primary breast cancer metastasis.

Applications

Unspecified application

Species

Unspecified reactive species

Chen Chen,Yusufu Maimaiti,Shen Zhijun,Liu Zeming,Guo Yawen,Yu Pan,Huang Tao

Disease models & mechanisms 10:655-669 PubMed28360125

2017

Cellular levels of Grb2 and cytoskeleton stability are correlated in a neurodegenerative scenario.

Applications

Unspecified application

Species

Unspecified reactive species

Piyali Majumder,Kasturi Roy,Brijesh Kumar Singh,Nihar Ranjan Jana,Debashis Mukhopadhyay

Evidence-based complementary and alternative medic 2016:8203789 PubMed27872653

2016

The Zuo Jin Wan Formula Induces Mitochondrial Apoptosis of Cisplatin-Resistant Gastric Cancer Cells via Cofilin-1.

Applications

Unspecified application

Species

Unspecified reactive species

Qing-Feng Tang,Jian Sun,Hui Yu,Xiao-Jing Shi,Rong Lv,Hong-Chang Wei,Pei-Hao Yin

PLoS pathogens 10:e1004351 PubMed25187968

2014

The cofilin phosphatase slingshot homolog 1 (SSH1) links NOD1 signaling to actin remodeling.

Applications

IP, WB

Species

Human, Human

Harald Bielig,Katja Lautz,Peter R Braun,Maureen Menning,Nikolaus Machuy,Christine Brügmann,Sandra Barisic,Stephan A Eisler,Maria Andree,Birte Zurek,Hamid Kashkar,Philippe J Sansonetti,Angelika Hausser,Thomas F Meyer,Thomas A Kufer

American journal of physiology. Lung cellular and 305:L651-64 PubMed24039253

2013

Thrombin selectively engages LIM kinase 1 and slingshot-1L phosphatase to regulate NF-κB activation and endothelial cell inflammation.

Applications

Unspecified application

Species

Unspecified reactive species

Antony Leonard,Catherine Marando,Arshad Rahman,Fabeha Fazal

The Journal of biological chemistry 286:35430-7 PubMed21857021

2011

Platelet-derived growth factor (PDGF) regulates Slingshot phosphatase activity via Nox1-dependent auto-dephosphorylation of serine 834 in vascular smooth muscle cells.

Applications

Species

Unspecified reactive species

Mithu Maheswaranathan,Hope K A Gole,Isabel Fernandez,Bernard Lassègue,Kathy K Griendling,Alejandra San Martín

View all publications

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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.

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Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

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Anti-Slingshot homolog 1/SSH1 antibody

Immunoprecipitation - Anti-Slingshot homolog 1/SSH1 antibody (AB76943)

Western blot - Anti-Slingshot homolog 1/SSH1 antibody (AB76943)

Key facts

Host species

Clonality

Isotype

Carrier free

Reacts with

Applications

Immunogen

Reactivity data

Properties and storage information

Form

Purification technique

Purification notes

Storage buffer

Shipped at conditions

Appropriate short-term storage conditions

Appropriate long-term storage conditions

Aliquoting information

Storage information

Supplementary information

Biological function summary

Pathways

Product protocols

Target data

Publications (12)

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