AB51523

Anti-SLIRP antibody

Name: Anti-SLIRP antibody (ab51523) | Abcam
Brand: Abcam Limited
SKU: AB51523
Price: 565 USD
Availability: InStock
Rating: 5 (4 reviews)

(4 Reviews)

(11 Publications)

Rabbit Polyclonal SLIRP antibody. Suitable for IHC-P, ICC/IF, WB and reacts with Human samples. Cited in 11 publications. Immunogen corresponding to Synthetic Peptide within Human SLIRP.

View Alternative Names

C14orf156, DC23, DC50, PD04872, SLIRP

3 Images

Immunocytochemistry/ Immunofluorescence - Anti-SLIRP antibody (AB51523)

ICC/IF

Supplier Data

Immunocytochemistry/ Immunofluorescence - Anti-SLIRP antibody (AB51523)

U2OS cells stained for SLIRP (green) using ab51523 at 1/500 dilution in immunocytochemistry. Nuclei and alpha-tubulin were counterstained with DAPI (blue) and Dylight 550 (red).

ICC/IF

Supplier Data

Immunocytochemistry/ Immunofluorescence - Anti-SLIRP antibody (AB51523)

HeLa cells stained for SLIRP using ab51523 at 1/200 dilution overnight at 4°C and detected with an anti-rabbit Dylight 488 (green) at 1/500 dilution in immunocytochemistry. Alpha tubulin was used as a co-stain at a 1/1000 dilution and detected with and anti-mouse Dylight 550 (red) at 1/500 dilution. Nuclei were counterstained with DAPI (blue).

Unknown

Western blot - Anti-SLIRP antibody (AB51523)

All lanes:

Western blot - Anti-SLIRP antibody (ab51523) at 0.6 µg/mL

All lanes:

human liver lysate

Predicted band size: 12 kDa

Observed band size: 12 kDa

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Key facts

Host species

Rabbit

Clonality

Polyclonal

Isotype

IgG

Carrier free

Reacts with

Human

Applications

ICC/IF, IHC-P, WB

applications

Immunogen

Synthetic Peptide within Human SLIRP. The exact immunogen used to generate this antibody is proprietary information.

Q9GZT3

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"}, "ICCIF" : {"fullname" : "Immunocytochemistry/ Immunofluorescence", "shortname":"ICC/IF"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "IHCP-species-checked": "guaranteed", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "ICCIF-species-checked": "testedAndGuaranteed", "ICCIF-species-dilution-info": "1/10 - 1/500", "ICCIF-species-notes": "", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "0.5-2 µg/mL", "WB-species-notes": "" }, "Mouse": { "IHCP-species-checked": "notRecommended", "IHCP-species-dilution-info": "1/100", "IHCP-species-notes": "", "ICCIF-species-checked": "notRecommended", "ICCIF-species-dilution-info": "1/10 - 1/500", "ICCIF-species-notes": "", "WB-species-checked": "notRecommended", "WB-species-dilution-info": "", "WB-species-notes": "" } } }

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Properties and storage information

Form

Liquid

Purification technique

Affinity purification Immunogen

Storage buffer

pH: 7.4 Preservative: 0.05% Sodium azide Constituents: Tris glycine, 0.87% Sodium chloride

Shipped at conditions

Blue Ice

Appropriate short-term storage duration

1-2 weeks

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

-20°C

Aliquoting information

Upon delivery aliquot

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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

SLIRP also known as SRA stem-loop interacting RNA binding protein is a small protein with a molecular mass of approximately 12 kDa. It has widespread expression in various tissues including the liver heart and muscle. SLIRP functions mainly in the mitochondria binding to RNA elements. This protein engages with the stem-loop structure in the SRA RNA influencing its stability and activity.

Biological function summary

SLIRP participates in transcriptional regulation through interaction with the steroid receptor RNA activator (SRA). It is an integral part of ribonucleoprotein complexes. SLIRP modulates gene expression by binding to nuclear receptors such as estrogen and androgen receptors. Through its RNA-binding ability it affects mitochondrial gene transcripts and subsequently influences mitochondrial function.

Pathways

SLIRP plays a role in the modulation of nuclear receptor signaling and mitochondrial biogenesis pathways. In the nuclear receptor signaling SLIRP interacts with SRA RNA impacting the activity of nuclear receptors. In mitochondrial biogenesis it is linked to the maintenance of mitochondrial gene expression. Proteins like PGC-1α which is an important regulator of energy metabolism interacts indirectly through the linked pathways.

SLIRP has implications in breast cancer and metabolic disorders. In breast cancer SLIRP's interaction with SRA RNA influences the behavior of estrogen receptors potentially affecting cancer progression. In metabolic disorders alterations in SLIRP levels can affect mitochondrial function connecting it to conditions like obesity or type 2 diabetes. Proteins such as PPARγ an important player in metabolic regulation may interplay with SLIRP functions in these contexts.

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
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Target data

RNA-binding protein that acts as a nuclear receptor corepressor. Probably acts by binding the SRA RNA, and repressing the SRA-mediated nuclear receptor coactivation. Binds the STR7 loop of SRA RNA. Also able to repress glucocorticoid (GR), androgen (AR), thyroid (TR) and VDR-mediated transactivation.

See full target information SLIRP

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Publications (11)

Recent publications for all applications. Explore the full list and refine your search

Journal of neuroinflammation 22:190 PubMed40684214

2025

Activated TBK1 promotes ACSL1-mediated microglia lipid droplet accumulation and neuroinflammation in Parkinson's disease.

Applications

Unspecified application

Species

Unspecified reactive species

Chunlei Han,Huizhi Wang,Moxuan Zhang,Liuliu Wu,Anni Wang,Pengda Yang,Yuan Gao,Chaonan Zhang,Jianing Gao,Tao Xue,Tingting Du,Yucheng Ji,Lin Shi,Chunkui Zhang,Renpeng Li,Yilei Xiao,Jianguo Zhang,Fangang Meng

Nature structural & molecular biology 31:1838-1847 PubMed39134711

2024

Structural basis of LRPPRC-SLIRP-dependent translation by the mitoribosome.

Applications

Unspecified application

Species

Unspecified reactive species

Vivek Singh,J Conor Moran,Yuzuru Itoh,Iliana C Soto,Flavia Fontanesi,Mary Couvillion,Martijn A Huynen,L Stirling Churchman,Antoni Barrientos,Alexey Amunts

Molecular and cellular neurosciences 129:103931 PubMed38508542

2024

Systemic inflammation activates coagulation and immune cell infiltration pathways in brains with propagating α-synuclein fibril aggregates.

Applications

Unspecified application

Species

Unspecified reactive species

Anne-Line Strange Laursen,Mikkel Vestergaard Olesen,Jonas Folke,Tomasz Brudek,Luisa Harriet Knecht,Florence Sotty,Kate Lykke Lambertsen,Karina Fog,Louise Torp Dalgaard,Susana Aznar

Frontiers in genetics 10:1393 PubMed32082360

2020

RNase H1 Regulates Mitochondrial Transcription and Translation the Degradation of 7S RNA.

Applications

Unspecified application

Species

Unspecified reactive species

Aurelio Reyes,Joanna Rusecka,Katarzyna Tońska,Massimo Zeviani

Scientific reports 9:18637 PubMed31819114

2019

Interaction between androgen receptor and coregulator SLIRP is regulated by Ack1 tyrosine kinase and androgen.

Applications

Unspecified application

Species

Unspecified reactive species

Dinuka De Silva,Zhentao Zhang,Yuanbo Liu,Joel S Parker,Chenxi Xu,Ling Cai,Gang Greg Wang,H Shelton Earp,Young E Whang

Molecular & cellular proteomics : MCP 13:2836-54 PubMed24855065

2014

Quantitative proteomic analysis of host-virus interactions reveals a role for Golgi brefeldin A resistance factor 1 (GBF1) in dengue infection.

Applications

Unspecified application

Species

Unspecified reactive species

Lindsay N Carpp,Richard S Rogers,Robert L Moritz,John D Aitchison

The Journal of biological chemistry 288:8321-31 PubMed23362268

2013

hCOA3 stabilizes cytochrome c oxidase 1 (COX1) and promotes cytochrome c oxidase assembly in human mitochondria.

Applications

Unspecified application

Species

Unspecified reactive species

Paula Clemente,Susana Peralta,Alberto Cruz-Bermudez,Lucía Echevarría,Flavia Fontanesi,Antoni Barrientos,Miguel A Fernandez-Moreno,Rafael Garesse

PLoS biology 10:e1001288 PubMed22448145

2012

Mutations in the mitochondrial methionyl-tRNA synthetase cause a neurodegenerative phenotype in flies and a recessive ataxia (ARSAL) in humans.

Applications

Species

Human

Vafa Bayat,Isabelle Thiffault,Manish Jaiswal,Martine Tétreault,Taraka Donti,Florin Sasarman,Geneviève Bernard,Julie Demers-Lamarche,Marie-Josée Dicaire,Jean Mathieu,Michel Vanasse,Jean-Pierre Bouchard,Marie-France Rioux,Charles M Lourenco,Zhihong Li,Claire Haueter,Eric A Shoubridge,Brett H Graham,Bernard Brais,Hugo J Bellen

Proceedings of the National Academy of Sciences of 107:11283-8 PubMed20534441

2010

Activator-Mediator binding regulates Mediator-cofactor interactions.

Applications

Species

Human

Christopher C Ebmeier,Dylan J Taatjes

Molecular biology of the cell 21:1315-23 PubMed20200222

2010

LRPPRC and SLIRP interact in a ribonucleoprotein complex that regulates posttranscriptional gene expression in mitochondria.

Applications

Species

Human

Florin Sasarman,Catherine Brunel-Guitton,Hana Antonicka,Timothy Wai,Eric A Shoubridge

View all publications

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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.

For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

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Anti-SLIRP antibody

Immunocytochemistry/ Immunofluorescence - Anti-SLIRP antibody (AB51523)

Immunocytochemistry/ Immunofluorescence - Anti-SLIRP antibody (AB51523)

Western blot - Anti-SLIRP antibody (AB51523)

Key facts

Host species

Clonality

Isotype

Carrier free

Reacts with

Applications

Immunogen

Reactivity data

Properties and storage information

Form

Purification technique

Storage buffer

Shipped at conditions

Appropriate short-term storage duration

Appropriate short-term storage conditions

Appropriate long-term storage conditions

Aliquoting information

Supplementary information

Biological function summary

Pathways

Product protocols

Target data

Publications (11)

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Primary Antibodies

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