Rabbit Recombinant Monoclonal SLIT1 antibody. Carrier free. Suitable for IHC-P and reacts with Transfected cell lysate, Mouse, Rat samples.
Images
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Slit1 antibody [EPR25107-4] - BSA and Azide free (AB307437), expandable thumbnail](https://content.abcam.com/products/images/slit1-antibody-epr25107-4-bsa-and-azide-free-ab307437--immunohistochemistry-formalin-pfa-fixed-paraffin-embedded-sections-img405689.jpg/_jcr_content/renditions/webp-475.webp "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Slit1 antibody [EPR25107-4] - BSA and Azide free (AB307437)")
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Slit1 antibody [EPR25107-4] - BSA and Azide free (AB307437), expandable thumbnail](https://content.abcam.com/products/images/slit1-antibody-epr25107-4-bsa-and-azide-free-ab307437--immunohistochemistry-formalin-pfa-fixed-paraffin-embedded-sections-img405688.jpg/_jcr_content/renditions/webp-475.webp "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Slit1 antibody [EPR25107-4] - BSA and Azide free (AB307437)")
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Slit1 antibody [EPR25107-4] - BSA and Azide free (AB307437), expandable thumbnail](https://content.abcam.com/products/images/slit1-antibody-epr25107-4-bsa-and-azide-free-ab307437--immunohistochemistry-formalin-pfa-fixed-paraffin-embedded-sections-img405687.jpg/_jcr_content/renditions/webp-475.webp "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Slit1 antibody [EPR25107-4] - BSA and Azide free (AB307437)")
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Slit1 antibody [EPR25107-4] - BSA and Azide free (AB307437), expandable thumbnail](https://content.abcam.com/products/images/slit1-antibody-epr25107-4-bsa-and-azide-free-ab307437--immunohistochemistry-formalin-pfa-fixed-paraffin-embedded-sections-img405686.jpg/_jcr_content/renditions/webp-475.webp "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Slit1 antibody [EPR25107-4] - BSA and Azide free (AB307437)")
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Slit1 antibody [EPR25107-4] - BSA and Azide free (AB307437), expandable thumbnail](https://content.abcam.com/products/images/slit1-antibody-epr25107-4-bsa-and-azide-free-ab307437--immunohistochemistry-formalin-pfa-fixed-paraffin-embedded-sections-img405685.jpg/_jcr_content/renditions/webp-475.webp "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Slit1 antibody [EPR25107-4] - BSA and Azide free (AB307437)")
Key facts
- Isotype
- IgG
- Host species
- Rabbit
- Storage buffer
pH: 7.2 - 7.4
Constituents: 100% PBS- Form
- Liquid
- Clonality
- Monoclonal
Immunogen
- The exact immunogen used to generate this antibody is proprietary information.
Reactivity data
Select an application| IP | Flow Cyt | ICC/IF | IHC-Fr | IHC-P | WB | |
|---|---|---|---|---|---|---|
| Human | Not recommended | Not recommended | Not recommended | Not recommended | Not recommended | Not recommended |
| Mouse | Not recommended | Not recommended | Not recommended | Not recommended | Tested | Not recommended |
| Rat | Not recommended | Not recommended | Not recommended | Not recommended | Tested | Not recommended |
| Transfected cell lysate | Not recommended | Not recommended | Not recommended | Not recommended | Tested | Not recommended |
IP
Not recommendedNot recommended
| Species | Dilution info | Notes |
|---|---|---|
Species Rat, Mouse, Human, Transfected cell lysate | Dilution info - | Notes - |
Flow Cyt
Not recommendedNot recommended
| Species | Dilution info | Notes |
|---|---|---|
Species Rat, Mouse, Human, Transfected cell lysate | Dilution info - | Notes - |
ICC/IF
Not recommendedNot recommended
| Species | Dilution info | Notes |
|---|---|---|
Species Rat, Mouse, Human, Transfected cell lysate | Dilution info - | Notes - |
IHC-Fr
Not recommendedNot recommended
| Species | Dilution info | Notes |
|---|---|---|
Species Mouse, Rat, Human, Transfected cell lysate | Dilution info - | Notes - |
IHC-P
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Transfected cell lysate | Dilution info - | Notes Perform heat-mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol. |
Species Mouse | Dilution info - | Notes Perform heat-mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol. |
Species Rat | Dilution info - | Notes Perform heat-mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol. |
Not recommendedNot recommended
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info - | Notes Perform heat-mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol. |
WB
Not recommendedNot recommended
| Species | Dilution info | Notes |
|---|---|---|
Species Rat, Mouse, Human, Transfected cell lysate | Dilution info - | Notes - |
Target data
Function
Thought to act as molecular guidance cue in cellular migration, and function appears to be mediated by interaction with roundabout homolog receptors. During neural development involved in axonal navigation at the ventral midline of the neural tube and projection of axons to different regions (By similarity). SLIT1 and SLIT2 together seem to be essential for midline guidance in the forebrain by acting as repulsive signal preventing inappropriate midline crossing by axons projecting from the olfactory bulb.
Alternative names
Kiaa0813, Slit1, Slit homolog 1 protein, Slit-1
Recommended products
Rabbit Recombinant Monoclonal SLIT1 antibody. Carrier free. Suitable for IHC-P and reacts with Transfected cell lysate, Mouse, Rat samples.
Key facts
- Isotype
- IgG
- Host species
- Rabbit
- Storage buffer
pH: 7.2 - 7.4
Constituents: 100% PBS- Form
- Liquid
- Clonality
- Monoclonal
- Immunogen
- The exact immunogen used to generate this antibody is proprietary information.
- Carrier free
- Yes
- Clone number
- EPR25107-4
- Purification technique
- Affinity purification Protein A
- Concentration
Storage
- Shipped at conditions
- Blue Ice
- Appropriate short-term storage conditions
- +4°C
- Appropriate long-term storage conditions
- +4°C
Notes
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free batch production
For more information, read more on recombinant antibodies.
Our carrier-free antibodies are typically supplied in a PBS-only formulation, purified and free of BSA, sodium azide and glycerol. The carrier-free buffer and high concentration allow for increased conjugation efficiency.
This conjugation-ready format is designed for use with fluorochromes, metal isotopes, oligonucleotides, and enzymes, which makes them ideal for antibody labelling, functional and cell-based assays, flow-based assays (e.g. mass cytometry) and Multiplex Imaging applications.
Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with 1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.
This product is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm, without the need for antibody preparation. Maxpar® is a trademark of Fluidigm Canada Inc.
Supplementary info
- This supplementary information is collated from multiple sources and compiled automatically.
- Activity summary
Slit1 also known as Slit homolog 1 protein is a large extracellular matrix protein with a mass of approximately 200 kDa. This protein is expressed in the developing and adult nervous system particularly in the brain. It contributes to axon guidance and cellular migration through its interaction with Robo receptors. By serving as a secreted ligand Slit1 plays a critical role in nervous system development guiding neurons to their correct positions.
- Biological function summary
Slit1 facilitates the formation of neural circuits by promoting axonal repulsion during neural development. It interacts as part of a receptor-ligand complex with Robo receptors on the cell surface which triggers intracellular signaling cascades. Slit1 also influences the shaping of synaptic connections and acts in a chemorepulsive manner ensuring appropriate neural wiring. Its presence aids cellular organization impacting cell division and movement in the nervous system.
- Pathways
Slit1 protein is an integral player in the axon guidance and neuronal network formation pathways. In these processes it modulates actin cytoskeleton dynamics and affects growth cone collapse through signaling with proteins like Robo1 and Robo2. The Slit-Robo signaling pathway works to coordinate cell movement and communication within the developing brain aligning with pathways that involve other guidance cues like Netrins and Semaphorins for balanced nervous system organization.
- Associated diseases and disorders
Slit1 has connections to neurological conditions such as schizophrenia and autism spectrum disorders. Its dysfunction can disrupt normal neural pathways contributing to improper brain connectivity and circuit formation. Slit1 interacts with other proteins implicated in these disorders such as Netrin-1 which supports its role in maintaining neural network integrity. Understanding Slit1's involvement provides insights into potential therapeutic targets for treating such developmental and neurological disorders.
Product promise
Tested
We have tested this species and application combination and it works. It is covered by our product promise.
Expected
We have not tested this specific species and application combination in-house, but expect it will work. It is covered by our product promise.
Predicted
This species and application combination has not been tested, but we predict it will work based on strong homology. However, this combination is not covered by our product promise.
Not recommended
We do not recommend this combination. It is not covered by our product promise.
We are dedicated to supporting your work with high quality reagents and we are here for you every step of the way should you need us.
In the unlikely event of one of our products not working as expected, you are covered by our product promise.
Full details and terms and conditions can be found here:
Terms & Conditions.
5 product images
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Slit1 antibody [EPR25107-4] - BSA and Azide free (ab307437), expandable thumbnail](https://content.abcam.com/products/images/slit1-antibody-epr25107-4-bsa-and-azide-free-ab307437--immunohistochemistry-formalin-pfa-fixed-paraffin-embedded-sections-img405689.jpg "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Slit1 antibody [EPR25107-4] - BSA and Azide free (ab307437)")
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Slit1 antibody [EPR25107-4] - BSA and Azide free (ab307437)
This data was developed using Anti-Slit1 antibody [EPR25107-4] ab307436, the same antibody clone in a different buffer formulation.
Immunohistochemical analysis of paraffin-embedded Rat whole-brain tissue labeling Slit1 with Anti-Slit1 antibody [EPR25107-4] ab307436 at 1/500 (1.1 ug/ml) followed by a ready to use LeicaDS9800 (Bond? Polymer Refine Detection) was used. Positive staining on rat cerebrum, while no staining on rat cerebellum (PMID: 11754167).The section was incubated with Anti-Slit1 antibody [EPR25107-4] ab307436 for 30 mins at room temperature.The immunostaining was performed on a Leica Biosystems BOND? RX instrument Counterstained with Hematoxylin.
Secondary antibody only control: Secondary antibody is a ready to use LeicaDS9800 (Bond? Polymer Refine Detection) was used.
Heat mediated antigen retrieval was performed with Tris-EDTA buffer (pH 9.0, Epitope Retrieval Solution2) for 20 mins![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Slit1 antibody [EPR25107-4] - BSA and Azide free (ab307437), expandable thumbnail](https://content.abcam.com/products/images/slit1-antibody-epr25107-4-bsa-and-azide-free-ab307437--immunohistochemistry-formalin-pfa-fixed-paraffin-embedded-sections-img405688.jpg "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Slit1 antibody [EPR25107-4] - BSA and Azide free (ab307437)")
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Slit1 antibody [EPR25107-4] - BSA and Azide free (ab307437)
This data was developed using Anti-Slit1 antibody [EPR25107-4] ab307436, the same antibody clone in a different buffer formulation.
Immunohistochemical analysis of paraffin-embedded Rat spleen tissue labeling Slit1 with Anti-Slit1 antibody [EPR25107-4] ab307436 at 1/500 (1.1 ug/ml) followed by a ready to use LeicaDS9800 (Bond? Polymer Refine Detection) was used. Negative control: no staining on rat spleen.The section was incubated with Anti-Slit1 antibody [EPR25107-4] ab307436 for 30 mins at room temperature.The immunostaining was performed on a Leica Biosystems BOND? RX instrument Counterstained with Hematoxylin.
Secondary antibody only control: Secondary antibody is a ready to use LeicaDS9800 (Bond? Polymer Refine Detection) was used.
Heat mediated antigen retrieval was performed with Tris-EDTA buffer (pH 9.0, Epitope Retrieval Solution2) for 20 mins![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Slit1 antibody [EPR25107-4] - BSA and Azide free (ab307437), expandable thumbnail](https://content.abcam.com/products/images/slit1-antibody-epr25107-4-bsa-and-azide-free-ab307437--immunohistochemistry-formalin-pfa-fixed-paraffin-embedded-sections-img405687.jpg "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Slit1 antibody [EPR25107-4] - BSA and Azide free (ab307437)")
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Slit1 antibody [EPR25107-4] - BSA and Azide free (ab307437)
This data was developed using Anti-Slit1 antibody [EPR25107-4] ab307436, the same antibody clone in a different buffer formulation.
Immunohistochemical analysis of paraffin-embedded Mouse spleen tissue labeling Slit1 with Anti-Slit1 antibody [EPR25107-4] ab307436 at 1/500 (1.1 ug/ml) followed by a ready to use LeicaDS9800 (Bond? Polymer Refine Detection) was used. Negative control: no staining on mouse spleen.The section was incubated with Anti-Slit1 antibody [EPR25107-4] ab307436 for 30 mins at room temperature.The immunostaining was performed on a Leica Biosystems BOND? RX instrument Counterstained with Hematoxylin.
Secondary antibody only control: Secondary antibody is a ready to use LeicaDS9800 (Bond? Polymer Refine Detection) was used.
Heat mediated antigen retrieval was performed with Tris-EDTA buffer (pH 9.0, Epitope Retrieval Solution2) for 20 mins![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Slit1 antibody [EPR25107-4] - BSA and Azide free (ab307437), expandable thumbnail](https://content.abcam.com/products/images/slit1-antibody-epr25107-4-bsa-and-azide-free-ab307437--immunohistochemistry-formalin-pfa-fixed-paraffin-embedded-sections-img405686.jpg "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Slit1 antibody [EPR25107-4] - BSA and Azide free (ab307437)")
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Slit1 antibody [EPR25107-4] - BSA and Azide free (ab307437)
This data was developed using Anti-Slit1 antibody [EPR25107-4] ab307436, the same antibody clone in a different buffer formulation.
Immunohistochemical analysis of paraffin-embedded Mouse cerebrum tissue labeling Slit1 with Anti-Slit1 antibody [EPR25107-4] ab307436 at 1/500 (1.1 ug/ml) followed by a ready to use LeicaDS9800 (Bond? Polymer Refine Detection) was used. Positive staining on mouse cerebrum.The section was incubated with Anti-Slit1 antibody [EPR25107-4] ab307436 for 30 mins at room temperature.The immunostaining was performed on a Leica Biosystems BOND? RX instrument Counterstained with Hematoxylin.
Secondary antibody only control: Secondary antibody is a ready to use LeicaDS9800 (Bond? Polymer Refine Detection) was used.
Heat mediated antigen retrieval was performed with Tris-EDTA buffer (pH 9.0, Epitope Retrieval Solution2) for 20 mins![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Slit1 antibody [EPR25107-4] - BSA and Azide free (ab307437), expandable thumbnail](https://content.abcam.com/products/images/slit1-antibody-epr25107-4-bsa-and-azide-free-ab307437--immunohistochemistry-formalin-pfa-fixed-paraffin-embedded-sections-img405685.jpg "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Slit1 antibody [EPR25107-4] - BSA and Azide free (ab307437)")
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Slit1 antibody [EPR25107-4] - BSA and Azide free (ab307437)
This data was developed using Anti-Slit1 antibody [EPR25107-4] ab307436, the same antibody clone in a different buffer formulation.
Immunohistochemical analysis of paraffin-embedded (A) HEK-293T cells t tissue labeling Slit1 with Anti-Slit1 antibody [EPR25107-4] ab307436 at 1/500 (1.1 ug/ml) followed by a ready to use LeicaDS9800 (Bond? Polymer Refine Detection) was used. Positive staining on (A) HEK-293T cells transfected with a SLIT1 expression vector containing a his tag. No staining on (B) HEK-293T cells transfected with empty vector containing a his tag.The section was incubated with Anti-Slit1 antibody [EPR25107-4] ab307436 for 30 mins at room temperature.The immunostaining was performed on a Leica Biosystems BOND? RX instrument Counterstained with Hematoxylin.
Secondary antibody only control: Secondary antibody is a ready to use LeicaDS9800 (Bond? Polymer Refine Detection) was used.
Heat mediated antigen retrieval was performed with Tris-EDTA buffer (pH 9.0, Epitope Retrieval Solution2) for 20 mins
Downloads
Product protocols
- Visit the general protocols
- Visit troubleshooting
Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.
For licensing inquiries, please contact partnerships@abcam.com