AB307437

Anti-Slit1 antibody [EPR25107-4] - BSA and Azide free

BOND RX™ Validated
RabMAb
Recombinant
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Rabbit Recombinant Monoclonal SLIT1 antibody. Carrier free. Suitable for IHC-P and reacts with Transfected cell lysate, Mouse, Rat samples.

View Alternative Names

Kiaa0813, Slit1, Slit homolog 1 protein, Slit-1

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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Slit1 antibody [EPR25107-4] - BSA and Azide free (AB307437)

IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Slit1 antibody [EPR25107-4] - BSA and Azide free (AB307437)

This data was developed using ab307436, the same antibody clone in a different buffer formulation. Immunohistochemical analysis of paraffin-embedded (A) HEK-293T cells t tissue labeling Slit1 with ab307436 at 1/500 (1.1 ug/ml) followed by a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection) was used. Positive staining on (A) HEK-293T cells transfected with a SLIT1 expression vector containing a his tag. No staining on (B) HEK-293T cells transfected with empty vector containing a his tag. The section was incubated with ab307436 for 30 mins at room temperature. The immunostaining was performed on a Leica Biosystems BOND® RX instrument Counterstained with Hematoxylin. Secondary antibody only control : Secondary antibody is a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection) was used. Heat mediated antigen retrieval was performed with Tris-EDTA buffer (pH 9.0, Epitope Retrieval Solution2) for 20 mins

IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Slit1 antibody [EPR25107-4] - BSA and Azide free (AB307437)

This data was developed using ab307436, the same antibody clone in a different buffer formulation. Immunohistochemical analysis of paraffin-embedded Rat whole-brain tissue labeling Slit1 with ab307436 at 1/500 (1.1 ug/ml) followed by a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection) was used. Positive staining on rat cerebrum, while no staining on rat cerebellum (PMID : 11754167). The section was incubated with ab307436 for 30 mins at room temperature. The immunostaining was performed on a Leica Biosystems BOND® RX instrument Counterstained with Hematoxylin. Secondary antibody only control : Secondary antibody is a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection) was used. Heat mediated antigen retrieval was performed with Tris-EDTA buffer (pH 9.0, Epitope Retrieval Solution2) for 20 mins

IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Slit1 antibody [EPR25107-4] - BSA and Azide free (AB307437)

This data was developed using ab307436, the same antibody clone in a different buffer formulation. Immunohistochemical analysis of paraffin-embedded Rat spleen tissue labeling Slit1 with ab307436 at 1/500 (1.1 ug/ml) followed by a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection) was used. Negative control : no staining on rat spleen. The section was incubated with ab307436 for 30 mins at room temperature.The immunostaining was performed on a Leica Biosystems BOND® RX instrument Counterstained with Hematoxylin. Secondary antibody only control : Secondary antibody is a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection) was used. Heat mediated antigen retrieval was performed with Tris-EDTA buffer (pH 9.0, Epitope Retrieval Solution2) for 20 mins

IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Slit1 antibody [EPR25107-4] - BSA and Azide free (AB307437)

This data was developed using ab307436, the same antibody clone in a different buffer formulation. Immunohistochemical analysis of paraffin-embedded Mouse spleen tissue labeling Slit1 with ab307436 at 1/500 (1.1 ug/ml) followed by a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection) was used. Negative control : no staining on mouse spleen. The section was incubated with ab307436 for 30 mins at room temperature.The immunostaining was performed on a Leica Biosystems BOND® RX instrument Counterstained with Hematoxylin. Secondary antibody only control : Secondary antibody is a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection) was used. Heat mediated antigen retrieval was performed with Tris-EDTA buffer (pH 9.0, Epitope Retrieval Solution2) for 20 mins

IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Slit1 antibody [EPR25107-4] - BSA and Azide free (AB307437)

This data was developed using ab307436, the same antibody clone in a different buffer formulation. Immunohistochemical analysis of paraffin-embedded Mouse cerebrum tissue labeling Slit1 with ab307436 at 1/500 (1.1 ug/ml) followed by a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection) was used. Positive staining on mouse cerebrum.The section was incubated with ab307436 for 30 mins at room temperature.The immunostaining was performed on a Leica Biosystems BOND® RX instrument Counterstained with Hematoxylin. Secondary antibody only control : Secondary antibody is a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection) was used. Heat mediated antigen retrieval was performed with Tris-EDTA buffer (pH 9.0, Epitope Retrieval Solution2) for 20 mins

Unconjugated

Anti-Slit1 antibody [EPR25107-4]

Key facts

Host species

Rabbit

Clonality

Monoclonal

Clone number

EPR25107-4

Isotype

IgG

Carrier free

Yes

Reacts with

Mouse, Rat

Applications

IHC-P

applications

Immunogen

The exact immunogen used to generate this antibody is proprietary information.

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Reactivity data

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Product details

Patented technology
Our RabMAb^® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb^® patents.

What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:

- High batch-to-batch consistency and reproducibility
- Improved sensitivity and specificity
- Long-term security of supply
- Animal-free batch production

For more information, read more on recombinant antibodies.

Conjugation ready
Our carrier-free antibodies are typically supplied in a PBS-only formulation, purified and free of BSA, sodium azide and glycerol. This conjugation-ready format is designed for use with fluorochromes, metal isotopes, oligonucleotides, and enzymes, which makes them ideal for antibody labelling, functional and cell-based assays, flow-based assays (e.g. mass cytometry) and Multiplex Imaging applications.

Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with 1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.

Compatibility
This product is compatible with the Maxpar^® Antibody Labeling Kit from Fluidigm, without the need for antibody preparation. Maxpar^® is a trademark of Fluidigm Canada Inc.

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Properties and storage information

Form

Liquid

Purification technique

Affinity purification Protein A

Storage buffer

pH: 7.2 - 7.4 Constituents: PBS

Shipped at conditions

Blue Ice

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

+4°C

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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

Slit1 also known as Slit homolog 1 protein is a large extracellular matrix protein with a mass of approximately 200 kDa. This protein is expressed in the developing and adult nervous system particularly in the brain. It contributes to axon guidance and cellular migration through its interaction with Robo receptors. By serving as a secreted ligand Slit1 plays a critical role in nervous system development guiding neurons to their correct positions.

Biological function summary

Slit1 facilitates the formation of neural circuits by promoting axonal repulsion during neural development. It interacts as part of a receptor-ligand complex with Robo receptors on the cell surface which triggers intracellular signaling cascades. Slit1 also influences the shaping of synaptic connections and acts in a chemorepulsive manner ensuring appropriate neural wiring. Its presence aids cellular organization impacting cell division and movement in the nervous system.

Pathways

Slit1 protein is an integral player in the axon guidance and neuronal network formation pathways. In these processes it modulates actin cytoskeleton dynamics and affects growth cone collapse through signaling with proteins like Robo1 and Robo2. The Slit-Robo signaling pathway works to coordinate cell movement and communication within the developing brain aligning with pathways that involve other guidance cues like Netrins and Semaphorins for balanced nervous system organization.

Slit1 has connections to neurological conditions such as schizophrenia and autism spectrum disorders. Its dysfunction can disrupt normal neural pathways contributing to improper brain connectivity and circuit formation. Slit1 interacts with other proteins implicated in these disorders such as Netrin-1 which supports its role in maintaining neural network integrity. Understanding Slit1's involvement provides insights into potential therapeutic targets for treating such developmental and neurological disorders.

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

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Target data

Thought to act as molecular guidance cue in cellular migration, and function appears to be mediated by interaction with roundabout homolog receptors. During neural development involved in axonal navigation at the ventral midline of the neural tube and projection of axons to different regions (By similarity). SLIT1 and SLIT2 together seem to be essential for midline guidance in the forebrain by acting as repulsive signal preventing inappropriate midline crossing by axons projecting from the olfactory bulb.

See full target information Slit1

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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.

For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com

Anti-Slit1 antibody [EPR25107-4] - BSA and Azide free

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Slit1 antibody [EPR25107-4] - BSA and Azide free (AB307437)

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Slit1 antibody [EPR25107-4] - BSA and Azide free (AB307437)

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Slit1 antibody [EPR25107-4] - BSA and Azide free (AB307437)

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Slit1 antibody [EPR25107-4] - BSA and Azide free (AB307437)

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Slit1 antibody [EPR25107-4] - BSA and Azide free (AB307437)

Related conjugates and formulations (1)

Key facts

Host species

Clonality

Clone number

Isotype

Carrier free

Reacts with

Applications

Immunogen

Complementary Products

Secondary Antibodies

Primary Antibodies

Primary Antibodies

Primary Antibodies

Reactivity data

Product details

Properties and storage information

Form

Purification technique

Storage buffer

Shipped at conditions

Appropriate short-term storage conditions

Appropriate long-term storage conditions

Supplementary information

Biological function summary

Pathways

Product protocols

Target data

Product promise