AB183704

Anti-Slit1 + Slit2 antibody [EP5796(N)]

RabMAb
What is this?

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(1 Publication)

Rabbit Monoclonal SLIT1 antibody. Suitable for WB and reacts with Human, Transfected cell lysate - Human samples. Cited in 1 publication.

View Alternative Names

KIAA0813, MEGF4, SLIL1, SLIT1, Slit homolog 1 protein, Slit-1, Multiple epidermal growth factor-like domains protein 4, Multiple EGF-like domains protein 4

3 Images

Supplier Data

Western blot - Anti-Slit1 + Slit2 antibody [EP5796(N)] (AB183704)

All lanes:

Western blot - Anti-Slit1 + Slit2 antibody [EP5796(N)] (ab183704) at 1/1000 dilution

Lane 1:

Human fetal brain lysate at 20 µg

Lane 2:

Human fetal forebrain lysate at 20 µg

Secondary

All lanes:

Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugate at 1/1000 dilution

Predicted band size: 168 kDa

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Supplier Data

Western blot - Anti-Slit1 + Slit2 antibody [EP5796(N)] (AB183704)

All lanes:

Western blot - Anti-Slit1 + Slit2 antibody [EP5796(N)] (ab183704) at 1/1000 dilution

All lanes:

SH-SY5Y cell lysate at 10 µg

Secondary

All lanes:

Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugate at 1/1000 dilution

Predicted band size: 168 kDa

false

Lab

Western blot - Anti-Slit1 + Slit2 antibody [EP5796(N)] (AB183704)

Blocking and diluting buffer and concentration : 5% NFDM/TBST

All lanes:

Western blot - Anti-Slit1 + Slit2 antibody [EP5796(N)] (ab183704) at 1/1000 dilution

Lane 1:

293T cells transfected with an empty vector containing a His tag whole cell lysate at 20 µg

Lane 2:

293T cells transfected with a human Slit1 expression vector containing a His-tag whole cell lysate at 20 µg

Lane 3:

293T cells transfected with a human Slit2 expression vector containing a His-tag whole cell lysate at 20 µg

Lane 4:

293T cells transfected with a human Slit3 expression vector containing a His-tag whole cell lysate at 20 µg

Secondary

All lanes:

Western blot - Goat Anti-Rabbit IgG H&L (HRP) (<a href='/en-us/products/secondary-antibodies/goat-rabbit-igg-h-l-hrp-ab97051'>ab97051</a>) at 1/20000 dilution

Observed band size: 250 kDa

false

Exposure time: 1s

Carrier free

Anti-Slit1 + Slit2 antibody [EP5796(N)] - BSA and Azide free

Key facts

Host species

Rabbit

Clonality

Monoclonal

Clone number

EP5796(N)

Isotype

IgG

Carrier free

Reacts with

Human

Applications

applications

Immunogen

The exact immunogen used to generate this antibody is proprietary information.

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/1000 - 1/2000", "WB-species-notes": "<p></p>" }, "Transfected cell lysate - Human": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/1000 - 1/2000", "WB-species-notes": "<p></p>" } } }

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Product details

Patented technology
Our RabMAb^® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb^® patents.

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Properties and storage information

Form

Liquid

Purification technique

Affinity purification Protein A

Storage buffer

pH: 7.2 Preservative: 0.01% Sodium azide Constituents: PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA

Shipped at conditions

Blue Ice

Appropriate short-term storage duration

1-2 weeks

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

-20°C

Aliquoting information

Upon delivery aliquot

Storage information

Avoid freeze / thaw cycle

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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

Slit1 also known as Slit homolog 1 protein is a large extracellular matrix protein with a mass of approximately 200 kDa. This protein is expressed in the developing and adult nervous system particularly in the brain. It contributes to axon guidance and cellular migration through its interaction with Robo receptors. By serving as a secreted ligand Slit1 plays a critical role in nervous system development guiding neurons to their correct positions.

Biological function summary

Slit1 facilitates the formation of neural circuits by promoting axonal repulsion during neural development. It interacts as part of a receptor-ligand complex with Robo receptors on the cell surface which triggers intracellular signaling cascades. Slit1 also influences the shaping of synaptic connections and acts in a chemorepulsive manner ensuring appropriate neural wiring. Its presence aids cellular organization impacting cell division and movement in the nervous system.

Pathways

Slit1 protein is an integral player in the axon guidance and neuronal network formation pathways. In these processes it modulates actin cytoskeleton dynamics and affects growth cone collapse through signaling with proteins like Robo1 and Robo2. The Slit-Robo signaling pathway works to coordinate cell movement and communication within the developing brain aligning with pathways that involve other guidance cues like Netrins and Semaphorins for balanced nervous system organization.

Slit1 has connections to neurological conditions such as schizophrenia and autism spectrum disorders. Its dysfunction can disrupt normal neural pathways contributing to improper brain connectivity and circuit formation. Slit1 interacts with other proteins implicated in these disorders such as Netrin-1 which supports its role in maintaining neural network integrity. Understanding Slit1's involvement provides insights into potential therapeutic targets for treating such developmental and neurological disorders.

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

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Target data

Thought to act as molecular guidance cue in cellular migration, and function appears to be mediated by interaction with roundabout homolog receptors. During neural development involved in axonal navigation at the ventral midline of the neural tube and projection of axons to different regions (By similarity). SLIT1 and SLIT2 together seem to be essential for midline guidance in the forebrain by acting as repulsive signal preventing inappropriate midline crossing by axons projecting from the olfactory bulb.

See full target information SLIT1

Additional targets

SLIT2

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Publications (1)

Recent publications for all applications. Explore the full list and refine your search

Journal of gastroenterology and hepatology 39:2892-2902 PubMed39343436

2024

RNA-binding protein Trx regulates alternative splicing and promotes metastasis of HCC via interacting with LINC00152.

Applications

Unspecified application

Species

Unspecified reactive species

Xiangnan Teng,Jin Shang,Lingyao Du,Wei Huang,Yonghong Wang,Miao Liu,Yuanji Ma,Ming Wang,Hong Tang,Lang Bai

View all publications

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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.

For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com

Anti-Slit1 + Slit2 antibody [EP5796(N)]

Western blot - Anti-Slit1 + Slit2 antibody [EP5796(N)] (AB183704)

Western blot - Anti-Slit1 + Slit2 antibody [EP5796(N)] (AB183704)

Western blot - Anti-Slit1 + Slit2 antibody [EP5796(N)] (AB183704)

Related conjugates and formulations (1)

Key facts

Host species

Clonality

Clone number

Isotype

Carrier free

Reacts with

Applications

Immunogen

Reactivity data

Product details

Properties and storage information

Form

Purification technique

Storage buffer

Shipped at conditions

Appropriate short-term storage duration

Appropriate short-term storage conditions

Appropriate long-term storage conditions

Aliquoting information

Storage information

Supplementary information

Biological function summary

Pathways

Product protocols

Target data

Additional targets

Publications (1)

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