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AB284838

Anti-Slit1 + Slit2 antibody [EP5796(N)] - BSA and Azide free

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Rabbit Monoclonal SLIT1 antibody. Carrier free. Suitable for WB and reacts with Human, Transfected cell lysate - Human samples.

View Alternative Names

KIAA0813, MEGF4, SLIL1, SLIT1, Slit homolog 1 protein, Slit-1, Multiple epidermal growth factor-like domains protein 4, Multiple EGF-like domains protein 4, SLIL3, SLIT2, Slit homolog 2 protein, Slit-2

3 Images
Western blot - Anti-Slit1 + Slit2 antibody [EP5796(N)] - BSA and Azide free (AB284838)
  • WB

Supplier Data

Western blot - Anti-Slit1 + Slit2 antibody [EP5796(N)] - BSA and Azide free (AB284838)

This data was developed using ab183704, the same antibody clone in a different buffer formulation.

All lanes:

Western blot - Anti-Slit1 + Slit2 antibody [EP5796(N)] (<a href='/en-us/products/primary-antibodies/slit1-slit2-antibody-ep5796n-ab183704'>ab183704</a>) at 1/1000 dilution

Lane 1:

Human fetal brain lysate at 20 µg

Lane 2:

Human fetal forebrain lysate at 20 µg

Secondary

All lanes:

Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugate at 1/1000 dilution

Predicted band size: 168 kDa

false

Western blot - Anti-Slit1 + Slit2 antibody [EP5796(N)] - BSA and Azide free (AB284838)
  • WB

Supplier Data

Western blot - Anti-Slit1 + Slit2 antibody [EP5796(N)] - BSA and Azide free (AB284838)

This data was developed using ab183704, the same antibody clone in a different buffer formulation.

All lanes:

Western blot - Anti-Slit1 + Slit2 antibody [EP5796(N)] (<a href='/en-us/products/primary-antibodies/slit1-slit2-antibody-ep5796n-ab183704'>ab183704</a>) at 1/1000 dilution

All lanes:

SH-SY5Y cell lysate at 10 µg

Secondary

All lanes:

Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugate at 1/1000 dilution

Predicted band size: 168 kDa

false

Western blot - Anti-Slit1 + Slit2 antibody [EP5796(N)] - BSA and Azide free (AB284838)
  • WB

Lab

Western blot - Anti-Slit1 + Slit2 antibody [EP5796(N)] - BSA and Azide free (AB284838)

Blocking and diluting buffer and concentration : 5% NFDM/TBST

All lanes:

Western blot - Anti-Slit1 + Slit2 antibody [EP5796(N)] (<a href='/en-us/products/primary-antibodies/slit1-slit2-antibody-ep5796n-ab183704'>ab183704</a>) at 1/1000 dilution

Lane 1:

293T cells transfected with an empty vector containing a His tag whole cell lysate at 20 µg

Lane 2:

293T cells transfected with a human Slit1 expression vector containing a His-tag whole cell lysate at 20 µg

Lane 3:

293T cells transfected with a human Slit2 expression vector containing a His-tag whole cell lysate at 20 µg

Lane 4:

293T cells transfected with a human Slit3 expression vector containing a His-tag whole cell lysate at 20 µg

Secondary

All lanes:

Western blot - Goat Anti-Rabbit IgG H&L (HRP) (<a href='/en-us/products/secondary-antibodies/goat-rabbit-igg-h-l-hrp-ab97051'>ab97051</a>) at 1/20000 dilution

Observed band size: 250 kDa

false

Exposure time: 1s

Key facts

Host species

Rabbit

Clonality

Monoclonal

Clone number

EP5796(N)

Isotype

IgG

Carrier free

Yes

Reacts with

Human

Applications

WB

applications

Immunogen

The exact immunogen used to generate this antibody is proprietary information.

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Primary Antibodies

AB15251

Anti-Wnt1 antibody

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  • 5
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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "", "WB-species-notes": "<p></p>" }, "Transfected cell lysate - Human": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "", "WB-species-notes": "<p></p>" } } }
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Product details

ab284838 is the carrier-free version of ab183704

Patented technology
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

Conjugation ready
Our carrier-free antibodies are typically supplied in a PBS-only formulation, purified and free of BSA, sodium azide and glycerol. This conjugation-ready format is designed for use with fluorochromes, metal isotopes, oligonucleotides, and enzymes, which makes them ideal for antibody labelling, functional and cell-based assays, flow-based assays (e.g. mass cytometry) and Multiplex Imaging applications.

Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with 1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.

Compatibility
This product is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm, without the need for antibody preparation. Maxpar® is a trademark of Fluidigm Canada Inc.

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Properties and storage information

Form
Liquid
Purification technique
Affinity purification Protein A
Storage buffer
pH: 7.2 - 7.4 Constituents: PBS
Shipped at conditions
Blue Ice
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
+4°C
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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

Slit1 also known as Slit homolog 1 protein is a large extracellular matrix protein with a mass of approximately 200 kDa. This protein is expressed in the developing and adult nervous system particularly in the brain. It contributes to axon guidance and cellular migration through its interaction with Robo receptors. By serving as a secreted ligand Slit1 plays a critical role in nervous system development guiding neurons to their correct positions.
Biological function summary

Slit1 facilitates the formation of neural circuits by promoting axonal repulsion during neural development. It interacts as part of a receptor-ligand complex with Robo receptors on the cell surface which triggers intracellular signaling cascades. Slit1 also influences the shaping of synaptic connections and acts in a chemorepulsive manner ensuring appropriate neural wiring. Its presence aids cellular organization impacting cell division and movement in the nervous system.

Pathways

Slit1 protein is an integral player in the axon guidance and neuronal network formation pathways. In these processes it modulates actin cytoskeleton dynamics and affects growth cone collapse through signaling with proteins like Robo1 and Robo2. The Slit-Robo signaling pathway works to coordinate cell movement and communication within the developing brain aligning with pathways that involve other guidance cues like Netrins and Semaphorins for balanced nervous system organization.

Slit1 has connections to neurological conditions such as schizophrenia and autism spectrum disorders. Its dysfunction can disrupt normal neural pathways contributing to improper brain connectivity and circuit formation. Slit1 interacts with other proteins implicated in these disorders such as Netrin-1 which supports its role in maintaining neural network integrity. Understanding Slit1's involvement provides insights into potential therapeutic targets for treating such developmental and neurological disorders.
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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:
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Target data

Thought to act as molecular guidance cue in cellular migration, and function appears to be mediated by interaction with roundabout homolog receptors. During neural development involved in axonal navigation at the ventral midline of the neural tube and projection of axons to different regions (By similarity). SLIT1 and SLIT2 together seem to be essential for midline guidance in the forebrain by acting as repulsive signal preventing inappropriate midline crossing by axons projecting from the olfactory bulb.
See full target information SLIT1

Additional targets

SLIT2
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Product promise

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For full details, please see our Terms & Conditions

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