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AB7665

Anti-Slit2 antibody

4

(6 Reviews)

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(16 Publications )

Rabbit Polyclonal SLIT2 antibody. Suitable for WB, ICC/IF, IHC-Fr and reacts with Chicken, Mouse, Rat, Human samples. Cited in 16 publications. Immunogen corresponding to Synthetic Peptide within Human SLIT2 aa 450-500 conjugated to Keyhole Limpet Haemocyanin.
1 Images
Western blot - Anti-Slit2 antibody (AB7665)
  • WB

Supplier Data

Western blot - Anti-Slit2 antibody (AB7665)

Molecular weight estimation was made by comparison to prestained molecular weight markers

All lanes:

Western blot - Anti-Slit2 antibody (ab7665) at 1/1350 dilution

All lanes:

Chicken spinal cord whole cell lysate at 30 µg

Secondary

All lanes:

IRDye™800conjugated Goat anti-Rabbit IgG [H&L] at 1/10000 dilution

Predicted band size: 169 kDa

Observed band size: 165 kDa

false

Key facts

Host species

Rabbit

Clonality

Polyclonal

Isotype

IgG

Carrier free

No

Reacts with

Chicken, Rat, Human, Mouse

Applications

ICC/IF, IHC-Fr, WB

applications

Immunogen

Synthetic Peptide within Human SLIT2 aa 450-500 conjugated to Keyhole Limpet Haemocyanin. The exact immunogen used to generate this antibody is proprietary information.

O94813

Specificity

<p>A BLAST analysis was used to suggest reactivity with this protein from human and chicken based on 100% homology for the immunogen sequence. Expect cross reactivity with SLIT-2 from Tetraodon nigroviridis , as only a single amino acid residue change is found within the immunogen sequence (94% positive by BLAST). The core 13 amino acids of the immunogen are also 100% homologous to SLIT-2 from mouse, rat, bovine and Xenopus laevis. Cross reactivity with SLIT-2 homologues from other sources has not been determined.</p>

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "ICCIF" : {"fullname" : "Immunocytochemistry/ Immunofluorescence", "shortname":"ICC/IF"}, "IHCFr" : {"fullname" : "Immunohistochemistry (Frozen sections)", "shortname":"IHC-Fr"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "", "ICCIF-species-checked": "guaranteed", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "<p></p>", "IHCFr-species-checked": "guaranteed", "IHCFr-species-dilution-info": "", "IHCFr-species-notes": "<p></p>" }, "Mouse": { "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "", "ICCIF-species-checked": "guaranteed", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "<p></p>", "IHCFr-species-checked": "guaranteed", "IHCFr-species-dilution-info": "", "IHCFr-species-notes": "<p></p>" }, "Rat": { "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "", "ICCIF-species-checked": "guaranteed", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "<p></p>", "IHCFr-species-checked": "guaranteed", "IHCFr-species-dilution-info": "", "IHCFr-species-notes": "<p></p>" }, "Chicken": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/500 - 1/2000", "WB-species-notes": "<p>Incubate primary antibody blocking buffer (5% goat serum + 0.5% non-fat dry milk in PBS) other blocking buffers cause a poor signal.</p>", "ICCIF-species-checked": "guaranteed", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "<p></p>", "IHCFr-species-checked": "guaranteed", "IHCFr-species-dilution-info": "", "IHCFr-species-notes": "<p></p>" }, "Xenopus laevis": { "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "", "ICCIF-species-checked": "predicted", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "", "IHCFr-species-checked": "predicted", "IHCFr-species-dilution-info": "", "IHCFr-species-notes": "" } } }
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Properties and storage information

Form
Liquid
Purification technique
Affinity purification Immunogen
Purification notes
This affinity purified antibody is directed against human SLIT-2 protein. The product was affinity purified from monospecific antiserum by immunoaffinity purification.
Storage buffer
pH: 7.2 Preservative: 0.01% Sodium azide Constituents: 0.88% Sodium chloride, 0.424% Potassium phosphate solution
Shipped at conditions
Blue Ice
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
-20°C
Aliquoting information
Upon delivery aliquot
Storage information
Avoid freeze / thaw cycle
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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

Slit2 also known as Slit homolog 2 protein is a guidance cue protein with a mass of approximately 200 kDa. It plays a role in axonal guidance and cellular migration. Slit2 is expressed in the central nervous system where it contributes to neural development. It is also found in various tissues such as the liver lungs and kidneys. Slit2 mediates its effects primarily through interaction with Roundabout (Robo) receptors.
Biological function summary

Slit2 functions in guiding axons during neuronal development and preventing them from straying from their pathways. It complexes with Robo receptors to transmit repulsive directional signals. This interaction is essential in the development of the nervous system ensuring proper neural circuit formation and synaptic connectivity. Besides neural guidance Slit2 also regulates other cellular events like angiogenesis and immune cell migration.

Pathways

Slit2 participates in the axon guidance pathway where it influences neural network structuring by interacting with Robo receptors and other guidance cues. The Slit-Robo pathway plays an important role in axonal repulsion working alongside the Netrin signaling pathway that involves proteins like DCC and UNC5. This way Slit2 and its pathways balance attractive and repulsive cues to form functional neuronal networks.

Slit2 has associations with cancers particularly glioblastoma where it affects tumor cell migration and invasion. Dysregulation of the Slit-Robo signaling may lead to enhanced cancer cell metastasis. Additionally Slit2 is linked to congenital heart diseases where its malfunctioning affects cardiac development. Its interaction with Robo and other proteins like Ephrin can influence these disease processes suggesting a broader role in both developmental and pathological contexts.
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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:
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Target data

Thought to act as molecular guidance cue in cellular migration, and function appears to be mediated by interaction with roundabout homolog receptors. During neural development involved in axonal navigation at the ventral midline of the neural tube and projection of axons to different regions. SLIT1 and SLIT2 seem to be essential for midline guidance in the forebrain by acting as repulsive signal preventing inappropriate midline crossing by axons projecting from the olfactory bulb. In spinal cord development may play a role in guiding commissural axons once they reached the floor plate by modulating the response to netrin. In vitro, silences the attractive effect of NTN1 but not its growth-stimulatory effect and silencing requires the formation of a ROBO1-DCC complex. May be implicated in spinal cord midline post-crossing axon repulsion. In vitro, only commissural axons that crossed the midline responded to SLIT2. In the developing visual system appears to function as repellent for retinal ganglion axons by providing a repulsion that directs these axons along their appropriate paths prior to, and after passage through, the optic chiasm. In vitro, collapses and repels retinal ganglion cell growth cones. Seems to play a role in branching and arborization of CNS sensory axons, and in neuronal cell migration. In vitro, Slit homolog 2 protein N-product, but not Slit homolog 2 protein C-product, repels olfactory bulb (OB) but not dorsal root ganglia (DRG) axons, induces OB growth cones collapse and induces branching of DRG axons. Seems to be involved in regulating leukocyte migration.
See full target information SLIT2

Alternative Names

SLIL3, SLIT2, Slit homolog 2 protein, Slit-2
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Publications (16)

Recent publications for all applications. Explore the full list and refine your search

Diabetes, metabolic syndrome and obesity : targets and therapy 17:4339-4354 PubMed39582785

2024

Quercetin Alleviates Diabetic Peripheral Neuropathy by Regulating Axon Guidance Factors and Inhibiting the Rho/ROCK Pathway in vivo and in vitro.

Applications

Unspecified application

Species

Unspecified reactive species

Wei Song,Yaoyang Li,Yifan Jia,Lingling Xu,Lin Kang,Yunshuang Yang,Shuyu Wang,Qian Zhang,Qunli Wu

Biomedicines 12: PubMed38255250

2024

Unraveling the Role of miR-200b-3p in Attention-Deficit/Hyperactivity Disorder (ADHD) and Its Therapeutic Potential in Spontaneously Hypertensive Rats (SHR).

Applications

Unspecified application

Species

Unspecified reactive species

Tung-Ming Chang,Hsiu-Ling Lin,Chih-Chen Tzang,Ju-An Liang,Tsai-Ching Hsu,Bor-Show Tzang

Scientific reports 13:7322 PubMed37147343

2023

An inducible model for genetic manipulation and fate-tracing of PDGFRβ-expressing fibrogenic cells in the liver.

Applications

Unspecified application

Species

Unspecified reactive species

Florian Hamberger,Young-Seon Mederacke,Ingmar Mederacke

Frontiers in physiology 11:228 PubMed32292352

2020

Slit2 Protects Hearts Against Ischemia-Reperfusion Injury by Inhibiting Inflammatory Responses and Maintaining Myofilament Contractile Properties.

Applications

Unspecified application

Species

Unspecified reactive species

Xiang Li,Shuang Zheng,Weijiang Tan,Hongqi Chen,Xiaohui Li,Jian Wu,Ting Luo,Xuecong Ren,W Glen Pyle,Lijing Wang,Peter H Backx,Ren Huang,Feng Hua Yang

DNA repair 70:55-66 PubMed30179733

2018

The influence of retinoic acid-induced differentiation on the radiation response of male germline stem cells.

Applications

Unspecified application

Species

Unspecified reactive species

Yi Zheng,Qijing Lei,Aldo Jongejan,Callista L Mulder,Saskia K M van Daalen,Sebastiaan Mastenbroek,Grace Hwang,Philip W Jordan,Sjoerd Repping,Geert Hamer

Cellular physiology and biochemistry : internation 46:890-906 PubMed29669322

2018

MicroRNA-381 Favors Repair of Nerve Injury Through Regulation of the SDF-1/CXCR4 Signaling Pathway via LRRC4 in Acute Cerebral Ischemia after Cerebral Lymphatic Blockage.

Applications

Unspecified application

Species

Unspecified reactive species

Jian-Min Piao,Wei Wu,Zhong-Xi Yang,Yu-Zhen Li,Qi Luo,Jin-Lu Yu

Experimental cell research 368:13-23 PubMed29649427

2018

VEGFR-3 signaling is regulated by a G-protein activator, activator of G-protein signaling 8, in lymphatic endothelial cells.

Applications

Unspecified application

Species

Human

Miho Sakima,Hisaki Hayashi,Abdullah Al Mamun,Motohiko Sato

Journal of cell science 130:2459-2467 PubMed28596239

2017

Electrophoresis of cell membrane heparan sulfate regulates galvanotaxis in glial cells.

Applications

Unspecified application

Species

Unspecified reactive species

Yu-Ja Huang,Paula Schiapparelli,Kristen Kozielski,Jordan Green,Emily Lavell,Hugo Guerrero-Cazares,Alfredo Quinones-Hinojosa,Peter Searson

International journal of molecular medicine 37:1139-45 PubMed26935869

2016

Slit-miR-218-Robo axis regulates retinal neovascularization.

Applications

Unspecified application

Species

Unspecified reactive species

Yichun Kong,Bei Sun,Quanhong Han,Shuang Han,Yuchuan Wang,Ying Chen

Scientific reports 5:16833 PubMed26582347

2015

Slit2N Inhibits Transmission of HIV-1 from Dendritic Cells to T-cells by Modulating Novel Cytoskeletal Elements.

Applications

Unspecified application

Species

Unspecified reactive species

Ashutosh Shrivastava,Anil Prasad,Paula M Kuzontkoski,Jinlong Yu,Jerome E Groopman
View all publications
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Product promise

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