Anti-Slit2 antibody [EPR23272-227] - BSA and Azide free

• WB

Lab

Western blot - Anti-Slit2 antibody [EPR23272-227] - BSA and Azide free (AB275092)

This data was developed using ab246503, the same antibody clone in a different buffer formulation.

Blocking and diluting buffer and concentration : 5% NFDM/TBST.

A~25 kDa degraded band is observed. Freshly made lysates can decrease the degradation.

The antibody detects Slit2-full(200KDa) and Slit2-N fragment (150KDa).

The molecular weight observed is consistent with what has been described in the literature (PMID : 11404413).

Negative control : T-47D (PMID : 17268810).

Exposure time :

Lanes 1-2 : 136 seconds;

Lanes 3-4 : 26 seconds.

All lanes:

Western blot - Anti-Slit2 antibody [EPR23272-227] (<a href='/en-us/products/primary-antibodies/slit2-antibody-epr23272-227-ab246503'>ab246503</a>) at 1/1000 dilution

Lane 1:

PC-3 (human prostate adenocarcinoma epithelial cell) whole cell lysate at 20 µg

Lane 2:

T-47D(Human ductal breast epithelial tumor epithelial cell), whole cell lysate at 20 µg

Lane 3:

mIMCD3 (mouse inner medlary collecting duct epithelial cell), whole cell lysate at 20 µL

Lane 4:

Mouse E14.5 brain tissue lysate at 20 µg

Secondary

All lanes:

Western blot - Goat Anti-Rabbit IgG H&L (HRP) (<a href='/en-us/products/secondary-antibodies/goat-rabbit-igg-h-l-hrp-ab97051'>ab97051</a>) at 1/20000 dilution

Predicted band size: 169 kDa

false

• WB

Lab

Western blot - Anti-Slit2 antibody [EPR23272-227] - BSA and Azide free (AB275092)

This data was developed using ab246503, the same antibody clone in a different buffer formulation.

Blocking and diluting buffer and concentration : 5% NFDM/TBST.

Fresh lysate was used in lane 1.

Band detected around 25KDa in lane 2 is caused by degradation as it disappeared in fresh lysate.

Exposure time : 37 seconds

All lanes:

Western blot - Anti-Slit2 antibody [EPR23272-227] (<a href='/en-us/products/primary-antibodies/slit2-antibody-epr23272-227-ab246503'>ab246503</a>) at 1/1000 dilution

All lanes:

293T (human embryonic kidney epithelial cell), whole cell lysate at 20 µg

Secondary

All lanes:

Western blot - Goat Anti-Rabbit IgG H&L (HRP) (<a href='/en-us/products/secondary-antibodies/goat-rabbit-igg-h-l-hrp-ab97051'>ab97051</a>) at 1/20000 dilution

Predicted band size: 169 kDa

false

• WB

Lab

Western blot - Anti-Slit2 antibody [EPR23272-227] - BSA and Azide free (AB275092)

This data was developed using ab246503, the same antibody clone in a different buffer formulation.

Blocking and diluting buffer and concentration : 5% NFDM/TBST.

The antibody detects Slit2-full(200KDa) and Slit2-N fragment (150KDa).

The molecular weight observed is consistent with what has been described in the literature (PMID : 11404413).

Exposure time : 3 minutes.

All lanes:

Western blot - Anti-Slit2 antibody [EPR23272-227] (<a href='/en-us/products/primary-antibodies/slit2-antibody-epr23272-227-ab246503'>ab246503</a>) at 1/1000 dilution

All lanes:

Rat brain tissue lysate at 20 µg

Secondary

All lanes:

Western blot - Goat Anti-Rabbit IgG H&L (HRP) (<a href='/en-us/products/secondary-antibodies/goat-rabbit-igg-h-l-hrp-ab97051'>ab97051</a>) at 1/20000 dilution

Predicted band size: 169 kDa

false

• WB

Lab

Western blot - Anti-Slit2 antibody [EPR23272-227] - BSA and Azide free (AB275092)

This data was developed using ab246503, the same antibody clone in a different buffer formulation. Blocking and diluting buffer and concentration : 5% NFDM/TBST. ab213204 was used as a His tag loading control.

All lanes:

Western blot - Anti-Slit2 antibody [EPR23272-227] (<a href='/en-us/products/primary-antibodies/slit2-antibody-epr23272-227-ab246503'>ab246503</a>) at 1/1000 dilution

Lane 1:

293T cells transfected with an empty vector containing a His tag whole cell lysate at 20 µg

Lane 2:

293T cells transfected with a human Slit1 expression vector containing a His-tag whole cell lysate at 20 µg

Lane 3:

293T cells transfected with a human Slit2 expression vector containing a His-tag whole cell lysate at 20 µg

Lane 4:

293T cells transfected with a human Slit3 expression vector containing a His-tag whole cell lysate at 20 µg

Secondary

All lanes:

Western blot - Goat Anti-Rabbit IgG H&L (HRP) (<a href='/en-us/products/secondary-antibodies/goat-rabbit-igg-h-l-hrp-ab97051'>ab97051</a>) at 1/20000 dilution

Observed band size: 250 kDa

false

Exposure time: 1s