Rabbit Recombinant Monoclonal SLIT2 antibody. Carrier free. Suitable for IHC-P, WB and reacts with Human, Transfected cell line - Human samples.
Images
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Slit2 antibody [EPR2771] - BSA and Azide free (AB240085), expandable thumbnail](https://content.abcam.com/products/images/slit2-antibody-epr2771-bsa-and-azide-free-ab240085--immunohistochemistry-formalin-pfa-fixed-paraffin-embedded-sections-img28496.jpg/_jcr_content/renditions/webp-475.webp "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Slit2 antibody [EPR2771] - BSA and Azide free (AB240085)")
![Western blot - Anti-Slit2 antibody [EPR2771] - BSA and Azide free (AB240085), expandable thumbnail](https://content.abcam.com/products/images/slit2-antibody-epr2771-bsa-and-azide-free-ab240085--western-blot-img441541.jpg/_jcr_content/renditions/webp-475.webp "Western blot - Anti-Slit2 antibody [EPR2771] - BSA and Azide free (AB240085)")
![Western blot - Anti-Slit2 antibody [EPR2771] - BSA and Azide free (AB240085), expandable thumbnail](https://content.abcam.com/products/images/slit2-antibody-epr2771-bsa-and-azide-free-ab240085--western-blot-img438536.jpg/_jcr_content/renditions/webp-475.webp "Western blot - Anti-Slit2 antibody [EPR2771] - BSA and Azide free (AB240085)")
![Western blot - Anti-Slit2 antibody [EPR2771] - BSA and Azide free (AB240085), expandable thumbnail](https://content.abcam.com/products/images/slit2-antibody-epr2771-bsa-and-azide-free-ab240085--western-blot-img419317.jpg/_jcr_content/renditions/webp-475.webp "Western blot - Anti-Slit2 antibody [EPR2771] - BSA and Azide free (AB240085)")
Key facts
- Isotype
- IgG
- Host species
- Rabbit
- Storage buffer
pH: 7.2 - 7.4
Constituents: PBS- Form
- Liquid
- Clonality
- Monoclonal
Immunogen
- The exact immunogen used to generate this antibody is proprietary information.
Reactivity data
Select an application| IHC-P | Flow Cyt | WB | ICC/IF | |
|---|---|---|---|---|
| Human | Tested | Not recommended | Tested | Not recommended |
| Mouse | Predicted | Not recommended | Predicted | Not recommended |
| Rat | Predicted | Not recommended | Predicted | Not recommended |
| Transfected cell line - Human | Not recommended | Not recommended | Tested | Not recommended |
IHC-P
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info - | Notes Perform heat-mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol. |
PredictedPredicted
| Species | Dilution info | Notes |
|---|---|---|
Species Mouse, Rat | Dilution info - | Notes - |
Not recommendedNot recommended
| Species | Dilution info | Notes |
|---|---|---|
Species Transfected cell line - Human | Dilution info - | Notes - |
Flow Cyt
Not recommendedNot recommended
| Species | Dilution info | Notes |
|---|---|---|
Species Human, Mouse, Rat, Transfected cell line - Human | Dilution info - | Notes - |
WB
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Human, Transfected cell line - Human | Dilution info - | Notes - |
PredictedPredicted
| Species | Dilution info | Notes |
|---|---|---|
Species Mouse, Rat | Dilution info - | Notes - |
ICC/IF
Not recommendedNot recommended
| Species | Dilution info | Notes |
|---|---|---|
Species Human, Mouse, Rat, Transfected cell line - Human | Dilution info - | Notes - |
Associated Products
Select an associated product type
2 products for Alternative Product
Target data
Function
Thought to act as molecular guidance cue in cellular migration, and function appears to be mediated by interaction with roundabout homolog receptors. During neural development involved in axonal navigation at the ventral midline of the neural tube and projection of axons to different regions. SLIT1 and SLIT2 seem to be essential for midline guidance in the forebrain by acting as repulsive signal preventing inappropriate midline crossing by axons projecting from the olfactory bulb. In spinal cord development may play a role in guiding commissural axons once they reached the floor plate by modulating the response to netrin. In vitro, silences the attractive effect of NTN1 but not its growth-stimulatory effect and silencing requires the formation of a ROBO1-DCC complex. May be implicated in spinal cord midline post-crossing axon repulsion. In vitro, only commissural axons that crossed the midline responded to SLIT2. In the developing visual system appears to function as repellent for retinal ganglion axons by providing a repulsion that directs these axons along their appropriate paths prior to, and after passage through, the optic chiasm. In vitro, collapses and repels retinal ganglion cell growth cones. Seems to play a role in branching and arborization of CNS sensory axons, and in neuronal cell migration. In vitro, Slit homolog 2 protein N-product, but not Slit homolog 2 protein C-product, repels olfactory bulb (OB) but not dorsal root ganglia (DRG) axons, induces OB growth cones collapse and induces branching of DRG axons. Seems to be involved in regulating leukocyte migration.
Alternative names
SLIL3, SLIT2, Slit homolog 2 protein, Slit-2
Recommended products
Rabbit Recombinant Monoclonal SLIT2 antibody. Carrier free. Suitable for IHC-P, WB and reacts with Human, Transfected cell line - Human samples.
Key facts
- Isotype
- IgG
- Host species
- Rabbit
- Storage buffer
pH: 7.2 - 7.4
Constituents: PBS- Form
- Liquid
- Clonality
- Monoclonal
- Immunogen
- The exact immunogen used to generate this antibody is proprietary information.
- Carrier free
- Yes
- Clone number
- EPR2771
- Purification technique
- Affinity purification Protein A
- Specificity
Slit2 is a secretory protein, so Brefeldin A (BFA) would help to increase the detection of Slit2 in cell lysate.
- Concentration
Storage
- Shipped at conditions
- Blue Ice
- Appropriate short-term storage conditions
- +4°C
- Appropriate long-term storage conditions
- +4°C
- Storage information
- Do Not Freeze
Notes
ab240085 is the carrier-free version of Anti-Slit2 antibody [EPR2771] ab134166.
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free batch production
For more information, read more on recombinant antibodies.
Our carrier-free antibodies are typically supplied in a PBS-only formulation, purified and free of BSA, sodium azide and glycerol. The carrier-free buffer and high concentration allow for increased conjugation efficiency.
This conjugation-ready format is designed for use with fluorochromes, metal isotopes, oligonucleotides, and enzymes, which makes them ideal for antibody labelling, functional and cell-based assays, flow-based assays (e.g. mass cytometry) and Multiplex Imaging applications.
Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with 1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.
This product is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm, without the need for antibody preparation. Maxpar® is a trademark of Fluidigm Canada Inc.
Supplementary info
- This supplementary information is collated from multiple sources and compiled automatically.
- Activity summary
Slit2 also known as Slit homolog 2 protein is a guidance cue protein with a mass of approximately 200 kDa. It plays a role in axonal guidance and cellular migration. Slit2 is expressed in the central nervous system where it contributes to neural development. It is also found in various tissues such as the liver lungs and kidneys. Slit2 mediates its effects primarily through interaction with Roundabout (Robo) receptors.
- Biological function summary
Slit2 functions in guiding axons during neuronal development and preventing them from straying from their pathways. It complexes with Robo receptors to transmit repulsive directional signals. This interaction is essential in the development of the nervous system ensuring proper neural circuit formation and synaptic connectivity. Besides neural guidance Slit2 also regulates other cellular events like angiogenesis and immune cell migration.
- Pathways
Slit2 participates in the axon guidance pathway where it influences neural network structuring by interacting with Robo receptors and other guidance cues. The Slit-Robo pathway plays an important role in axonal repulsion working alongside the Netrin signaling pathway that involves proteins like DCC and UNC5. This way Slit2 and its pathways balance attractive and repulsive cues to form functional neuronal networks.
- Associated diseases and disorders
Slit2 has associations with cancers particularly glioblastoma where it affects tumor cell migration and invasion. Dysregulation of the Slit-Robo signaling may lead to enhanced cancer cell metastasis. Additionally Slit2 is linked to congenital heart diseases where its malfunctioning affects cardiac development. Its interaction with Robo and other proteins like Ephrin can influence these disease processes suggesting a broader role in both developmental and pathological contexts.
Product promise
Tested
We have tested this species and application combination and it works. It is covered by our product promise.
Expected
We have not tested this specific species and application combination in-house, but expect it will work. It is covered by our product promise.
Predicted
This species and application combination has not been tested, but we predict it will work based on strong homology. However, this combination is not covered by our product promise.
Not recommended
We do not recommend this combination. It is not covered by our product promise.
We are dedicated to supporting your work with high quality reagents and we are here for you every step of the way should you need us.
In the unlikely event of one of our products not working as expected, you are covered by our product promise.
Full details and terms and conditions can be found here:
Terms & Conditions.
4 product images
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Slit2 antibody [EPR2771] - BSA and Azide free (ab240085), expandable thumbnail](https://content.abcam.com/products/images/slit2-antibody-epr2771-bsa-and-azide-free-ab240085--immunohistochemistry-formalin-pfa-fixed-paraffin-embedded-sections-img28496.jpg "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Slit2 antibody [EPR2771] - BSA and Azide free (ab240085)")
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Slit2 antibody [EPR2771] - BSA and Azide free (ab240085)
Immunohistochemical analysis of paraffin embedded Human fetal kidney tissue labelled with Anti-Slit2 antibody [EPR2771] ab134166 at 1/100 dilution.
This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (Anti-Slit2 antibody [EPR2771] ab134166).
Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
![Western blot - Anti-Slit2 antibody [EPR2771] - BSA and Azide free (ab240085), expandable thumbnail](https://content.abcam.com/products/images/slit2-antibody-epr2771-bsa-and-azide-free-ab240085--western-blot-img441541.jpg "Western blot - Anti-Slit2 antibody [EPR2771] - BSA and Azide free (ab240085)")
Western blot - Anti-Slit2 antibody [EPR2771] - BSA and Azide free (ab240085)
This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (Anti-Slit2 antibody [EPR2771] ab134166).
Blocking and diluting buffer and concentration: 5% NFDM/TBST.
Anti-6X His tag® antibody [EPR20547] - ChIP Grade ab213204 was used as a His tag loading control.
All lanes: Western blot - Anti-Slit2 antibody [EPR2771] (Anti-Slit2 antibody [EPR2771] ab134166) at 1/1000 dilution
Lane 1: 293T cells transfected with an empty vector containing a His tag whole cell lysate at 20 µg
Lane 2: 293T cells transfected with a human Slit1 expression vector containing a His-tag whole cell lysate at 20 µg
Lane 3: 293T cells transfected with a human Slit2 expression vector containing a His-tag whole cell lysate at 20 µg
Lane 4: 293T cells transfected with a human Slit3 expression vector containing a His-tag whole cell lysate at 20 µg
SecondaryAll lanes: Western blot - Goat Anti-Rabbit IgG H&L (HRP) (Goat Anti-Rabbit IgG H&L (HRP) ab97051) at 1/20000 dilution
Observed band size: 250 kDa
Exposure time: 1s
![Western blot - Anti-Slit2 antibody [EPR2771] - BSA and Azide free (ab240085), expandable thumbnail](https://content.abcam.com/products/images/slit2-antibody-epr2771-bsa-and-azide-free-ab240085--western-blot-img438536.jpg "Western blot - Anti-Slit2 antibody [EPR2771] - BSA and Azide free (ab240085)")
Western blot - Anti-Slit2 antibody [EPR2771] - BSA and Azide free (ab240085)
This data was developed using Anti-Slit2 antibody [EPR2771] ab134166, the same antibody clone in a different buffer formulation.
Anti-Slit2 antibody [EPR2771] ab134166 was shown to react with SLIT2 in wild-type HAP1 cells in Western blot with loss of signal observed in a SLIT2 knockout cell line. Wild-type HAP1 and SLIT2 knockout cell lysates were subjected to SDS-PAGE. Membranes were blocked in 5% milk in TBST for 1 hr before incubation with Anti-Slit2 antibody [EPR2771] ab134166 overnight at 4 °C at a 1/1000 dilution. Blots were incubated with secondary antibodies at 0.2 µg/mL before imaging.
These data were provided by YCharOS Inc., an open science company with the mission of characterizing commercially available antibody reagents for all human proteins. Abcam and YCharOS are working together to help address the reproducibility crisis by enabling the life science community to better evaluate commercially available antibodies.All lanes: Western blot - Anti-Slit2 antibody [EPR2771] (Anti-Slit2 antibody [EPR2771] ab134166) at 1/1000 dilution
Lane 1: Wild-type HAP1 lysate at 40 µg
Lane 2: SLIT2 knock-out HAP1 lysate at 40 µg
![Western blot - Anti-Slit2 antibody [EPR2771] - BSA and Azide free (ab240085), expandable thumbnail](https://content.abcam.com/products/images/slit2-antibody-epr2771-bsa-and-azide-free-ab240085--western-blot-img419317.jpg "Western blot - Anti-Slit2 antibody [EPR2771] - BSA and Azide free (ab240085)")
Western blot - Anti-Slit2 antibody [EPR2771] - BSA and Azide free (ab240085)
Blocking buffer and concentration: 5% NFDM/TBST
Diluting buffer and concentration: 5% NFDM/TBST
The expression level of Slit2 in T-47D and A549 are consistent with describes in PMID: 24287947 and PMID: 12384551.
Negative control: T-47D and A549 (PMID: 24287947 and PMID: 12384551).
Slit2 is a secretory protein, so Brefeldin A (BFA) would help to increase the detection of Slit2 in cell lysate.
Anti-GAPDH antibody [EPR16891] - Loading Control ab181602 was used as a loading control.
This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (Anti-Slit2 antibody [EPR2771] ab134166).All lanes: Western blot - Anti-Slit2 antibody [EPR2771] (Anti-Slit2 antibody [EPR2771] ab134166) at 1/1000 dilution
Lane 1: HEK-293 (Human embryonic kidney epithelial cell) whole cell lysate at 20 µg
Lane 2: T-47(Human ductal breast epithelial tumor epithelial cell) whole cell lysate at 20 µg
Lane 3: A549 (Human lung carcinoma epithelial cell) whole cell lysate at 20 µg
Lane 4: Untreated PC-3 (Human prostate adenocarcinoma epithelial cell) whole cell lysate at 20 µg
Lane 5: PC-3 (Human prostate adenocarcinoma epithelial cell) treated with 300ng/ml Brefeldin A (BFA) for 24 hours whole cell lysate at 20 µg
SecondaryAll lanes: Western blot - Goat Anti-Rabbit IgG H&L (HRP) (Goat Anti-Rabbit IgG H&L (HRP) ab97051) at 1/20000 dilution
Predicted band size: 169 kDa
Observed band size: 200 kDa
Exposure time: 40s
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