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Rabbit Recombinant Monoclonal SLIT2 antibody. Carrier free. Suitable for IHC-P, WB and reacts with Human samples.

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Images

Key facts

Isotype

IgG

Host species

Rabbit

Storage buffer

pH: 7.2 - 7.4
Constituents: PBS

Form

Liquid

Clonality

Monoclonal

Immunogen

  • The exact immunogen used to generate this antibody is proprietary information.
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Reactivity data

Select an application
Product promiseTestedExpectedPredictedNot recommended
IHC-PFlow CytWBICC/IF
Human
Tested
Not recommended
Tested
Not recommended
Mouse
Predicted
Not recommended
Predicted
Not recommended
Rat
Predicted
Not recommended
Predicted
Not recommended

Tested
Tested

Species

Human

Dilution info

-

Notes

Perform heat-mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

Predicted
Predicted

Species

Mouse, Rat

Dilution info

-

Notes

-

Not recommended
Not recommended

Species

Human, Mouse, Rat

Dilution info

-

Notes

-

Tested
Tested

Species

Human

Dilution info

-

Notes

-

Predicted
Predicted

Species

Mouse, Rat

Dilution info

-

Notes

-

Not recommended
Not recommended

Species

Human, Mouse, Rat

Dilution info

-

Notes

-

Target data

Function

Thought to act as molecular guidance cue in cellular migration, and function appears to be mediated by interaction with roundabout homolog receptors. During neural development involved in axonal navigation at the ventral midline of the neural tube and projection of axons to different regions. SLIT1 and SLIT2 seem to be essential for midline guidance in the forebrain by acting as repulsive signal preventing inappropriate midline crossing by axons projecting from the olfactory bulb. In spinal chord development may play a role in guiding commissural axons once they reached the floor plate by modulating the response to netrin. In vitro, silences the attractive effect of NTN1 but not its growth-stimulatory effect and silencing requires the formation of a ROBO1-DCC complex. May be implicated in spinal chord midline post-crossing axon repulsion. In vitro, only commissural axons that crossed the midline responded to SLIT2. In the developing visual system appears to function as repellent for retinal ganglion axons by providing a repulsion that directs these axons along their appropriate paths prior to, and after passage through, the optic chiasm. In vitro, collapses and repels retinal ganglion cell growth cones. Seems to play a role in branching and arborization of CNS sensory axons, and in neuronal cell migration. In vitro, Slit homolog 2 protein N-product, but not Slit homolog 2 protein C-product, repels olfactory bulb (OB) but not dorsal root ganglia (DRG) axons, induces OB growth cones collapse and induces branching of DRG axons. Seems to be involved in regulating leukocyte migration.

Alternative names

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Rabbit Recombinant Monoclonal SLIT2 antibody. Carrier free. Suitable for IHC-P, WB and reacts with Human samples.

Alternative names

Key facts

Isotype

IgG

Form

Liquid

Clonality

Monoclonal

Immunogen
  • The exact immunogen used to generate this antibody is proprietary information.
Carrier free

Yes

Clone number

EPR2771

Purification technique

Affinity purification Protein A

Specificity

Slit2 is a secretory protein, so Brefeldin A (BFA) would help to increase the detection of Slit2 in cell lysate.

Concentration
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Storage

Shipped at conditions

Blue Ice

Appropriate long-term storage conditions

+4°C

Storage information

Do Not Freeze

Notes

ab240085 is the carrier-free version of ab134166.

Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

This product is a recombinant monoclonal antibody, which offers several advantages including:

  • - High batch-to-batch consistency and reproducibility
  • - Improved sensitivity and specificity
  • - Long-term security of supply
  • - Animal-free batch production

For more information, read more on recombinant antibodies.

Our carrier-free antibodies are typically supplied in a PBS-only formulation, purified and free of BSA, sodium azide and glycerol. The carrier-free buffer and high concentration allow for increased conjugation efficiency.

This conjugation-ready format is designed for use with fluorochromes, metal isotopes, oligonucleotides, and enzymes, which makes them ideal for antibody labelling, functional and cell-based assays, flow-based assays (e.g. mass cytometry) and Multiplex Imaging applications.

Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with 1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.

This product is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm, without the need for antibody preparation. Maxpar® is a trademark of Fluidigm Canada Inc.

Supplementary info

Biological function summary

Slit2 functions in guiding axons during neuronal development and preventing them from straying from their pathways. It complexes with Robo receptors to transmit repulsive directional signals. This interaction is essential in the development of the nervous system ensuring proper neural circuit formation and synaptic connectivity. Besides neural guidance Slit2 also regulates other cellular events like angiogenesis and immune cell migration.

Activity summary

Slit2 also known as Slit homolog 2 protein is a guidance cue protein with a mass of approximately 200 kDa. It plays a role in axonal guidance and cellular migration. Slit2 is expressed in the central nervous system where it contributes to neural development. It is also found in various tissues such as the liver lungs and kidneys. Slit2 mediates its effects primarily through interaction with Roundabout (Robo) receptors.

Pathways

Slit2 participates in the axon guidance pathway where it influences neural network structuring by interacting with Robo receptors and other guidance cues. The Slit-Robo pathway plays an important role in axonal repulsion working alongside the Netrin signaling pathway that involves proteins like DCC and UNC5. This way Slit2 and its pathways balance attractive and repulsive cues to form functional neuronal networks.

Associated diseases and disorders

Slit2 has associations with cancers particularly glioblastoma where it affects tumor cell migration and invasion. Dysregulation of the Slit-Robo signaling may lead to enhanced cancer cell metastasis. Additionally Slit2 is linked to congenital heart diseases where its malfunctioning affects cardiac development. Its interaction with Robo and other proteins like Ephrin can influence these disease processes suggesting a broader role in both developmental and pathological contexts.

Product promise

We are dedicated to supporting your work with high quality reagents and we are here for you every step of the way should you need us.

In the unlikely event of one of our products not working as expected, you are covered by our product promise.

Full details and terms and conditions can be found here:
Terms & Conditions.

3 product images

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Slit2 antibody [EPR2771] - BSA and Azide free (ab240085), expandable thumbnail

    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Slit2 antibody [EPR2771] - BSA and Azide free (ab240085)

    Immunohistochemical analysis of paraffin embedded Human fetal kidney tissue labelled with ab134166 at 1/100 dilution.

    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab134166).

    Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

  • Western blot - Anti-Slit2 antibody [EPR2771] - BSA and Azide free (ab240085), expandable thumbnail

    Western blot - Anti-Slit2 antibody [EPR2771] - BSA and Azide free (ab240085)

    This data was developed using ab134166, the same antibody clone in a different buffer formulation.

    ab134166 was shown to react with SLIT2 in wild-type HAP1 cells in Western blot with loss of signal observed in a SLIT2 knockout cell line. Wild-type HAP1 and SLIT2 knockout cell lysates were subjected to SDS-PAGE. Membranes were blocked in 5% milk in TBST for 1 hr before incubation with ab134166 overnight at 4 °C at a 1/1000 dilution. Blots were incubated with secondary antibodies at 0.2 µg/mL before imaging.

    These data were provided by YCharOS Inc., an open science company with the mission of characterizing commercially available antibody reagents for all human proteins. Abcam and YCharOS are working together to help address the reproducibility crisis by enabling the life science community to better evaluate commercially available antibodies.

    All lanes: Western blot - Anti-Slit2 antibody [EPR2771] (AB134166) at 1/1000 dilution

    Lane 1: Wild-type HAP1 lysate at 40 µg

    Lane 2: SLIT2 knock-out HAP1 lysate at 40 µg

  • Western blot - Anti-Slit2 antibody [EPR2771] - BSA and Azide free (ab240085), expandable thumbnail

    Western blot - Anti-Slit2 antibody [EPR2771] - BSA and Azide free (ab240085)

    Blocking buffer and concentration: 5% NFDM/TBST 

    Diluting buffer and concentration: 5% NFDM/TBST

    The expression level of Slit2 in T-47D and A549 are consistent with describes in PMID: 24287947 and PMID: 12384551.

    Negative control:  T-47D and A549 (PMID: 24287947 and PMID: 12384551).

    Slit2 is a secretory protein, so Brefeldin A (BFA) would help to increase the detection of Slit2 in cell lysate.

    ab181602 was used as a loading control.
    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab134166).

    All lanes: Western blot - Anti-Slit2 antibody [EPR2771] (AB134166) at 1/1000 dilution

    Lane 1: HEK-293 (Human embryonic kidney epithelial cell) whole cell lysate at 20 µg

    Lane 2: T-47(Human ductal breast epithelial tumor epithelial cell) whole cell lysate at 20 µg

    Lane 3: A549 (Human lung carcinoma epithelial cell) whole cell lysate at 20 µg

    Lane 4: Untreated PC-3 (Human prostate adenocarcinoma epithelial cell) whole cell lysate at 20 µg

    Lane 5: PC-3 (Human prostate adenocarcinoma epithelial cell) treated with 300ng/ml Brefeldin A (BFA) for 24 hours whole cell lysate at 20 µg

    Secondary

    All lanes: Western blot - Goat Anti-Rabbit IgG H&L (HRP) (AB97051) at 1/20000 dilution

    Predicted band size: 169 kDa

    Observed band size: 200 kDa

    Exposure time: 40s

    Blocking buffer and concentration: 5% NFDM/TBST 

    Diluting buffer and concentration: 5% NFDM/TBST

    The expression level of Slit2 in T-47D and A549 are consistent with describes in PMID: 24287947 and PMID: 12384551.

    Negative control:  T-47D and A549 (PMID: 24287947 and PMID: 12384551).

    Slit2 is a secretory protein, so Brefeldin A (BFA) would help to increase the detection of Slit2 in cell lysate.

    ab181602 was used as a loading control.

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can:

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

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