JavaScript is disabled in your browser. Please enable JavaScript to view this website.
AB11018

Anti-Slit3 antibody

Be the first to review this product! Submit a review

|

(2 Publications)

Rabbit Polyclonal SLIT3 antibody. Suitable for WB and reacts with Cow samples. Cited in 2 publications. Immunogen corresponding to Synthetic Peptide within Human SLIT3 aa 1150-1200 conjugated to Keyhole Limpet Haemocyanin.

View Alternative Names

KIAA0814, MEGF5, SLIL2, UNQ691/PRO1336, SLIT3, Slit homolog 3 protein, Slit-3, Multiple epidermal growth factor-like domains protein 5, Multiple EGF-like domains protein 5

1 Images
Western blot - Anti-Slit3 antibody (AB11018)
  • WB

Unknown

Western blot - Anti-Slit3 antibody (AB11018)

All lanes:

Western blot - Anti-Slit3 antibody (ab11018) at 1/800 dilution

All lanes:

Bovine thyroid whole cell lysate at 35 µg

Secondary

All lanes:

Goat-anti-Rabbit IgG [H&L] at 1/10000 dilution

Predicted band size: 168 kDa

Observed band size: 145 kDa

false

Key facts

Host species

Rabbit

Clonality

Polyclonal

Isotype

IgG

Carrier free

No

Reacts with

Cow

Applications

WB

applications

Immunogen

Synthetic Peptide within Human SLIT3 aa 1150-1200 conjugated to Keyhole Limpet Haemocyanin. The exact immunogen used to generate this antibody is proprietary information.

O75094

Specificity

This affinity purified antibody is directed against human SLIT-3 protein. The product was affinity purified from monospecific antiserum by immunoaffinity purification. A BLAST analysis was used to suggest reactivity with this protein from human, rat, dog, bovine and mouse based on 100% homology for the immunogen sequence. Expect cross reactivity with SLIT-3 from chimpanzee and chicken sources, as only a single amino acid residue change is found within the immunogen sequence (93% positive by BLAST). Cross reactivity with SLIT-3 homologues from other sources has not been determined.

style
left-column

Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "" }, "Mouse": { "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "" }, "Rat": { "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "" }, "Cow": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/500 - 1/2000", "WB-species-notes": "<p></p>" } } }
style
left-column
style
left-column

Properties and storage information

Form
Liquid
Purification technique
Affinity purification Immunogen
Purification notes
This affinity purified antibody is directed against human SLIT-3 protein. The product was affinity purified from monospecific antiserum by immunoaffinity purification.
Storage buffer
pH: 7.2 Preservative: 0.01% Sodium azide Constituents: 0.88% Sodium chloride, 0.424% Potassium phosphate solution
Shipped at conditions
Blue Ice
Appropriate short-term storage duration
1-2 weeks
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
-20°C
Aliquoting information
Upon delivery aliquot
Storage information
Avoid freeze / thaw cycle
style
left-column

Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

Slit3 also known as Slit Homolog 3 is a secreted protein encoded by the SLIT3 gene. It belongs to the Slit family of proteins and has a mass of approximately 160 kDa. These proteins typically have a leucine-rich domain and immunoglobulin-like repeats. Slit3 is expressed in various tissues including the heart lung and kidney but shows highest expression in the fetal lung. It operates in the extracellular space where it interacts with cell surface receptors to relay information essential for cellular functions.
Biological function summary

Slit3 regulates cellular migration and axon guidance by binding to Roundabout (Robo) receptors. This interaction plays a critical role in neuronal development and vascularization processes. In addition Slit3 can influence endothelial cell behavior and is a part of the axon guidance complex. By contributing to angiogenesis and neural patterning Slit3 impacts both developmental and repair mechanisms within the organism.

Pathways

The Slit3 protein participates in the axon guidance and angiogenesis signaling pathways. Slit3 interacts with Robo receptors to mediate cellular repulsion and navigation of the growth cones influencing nervous system architecture. The protein is closely related to other Slit family members particularly Slit1 and Slit2 which share similar roles in regulating guidance cues and vascular formation ensuring proper tissue development and injury response.

Disruptive mutations or altered expression of Slit3 have been linked to congenital diaphragmatic hernias and tumorigenesis. Congenital diaphragmatic hernia a defect that affects the diaphragm development connects to abnormal Slit3-mediated signaling that impacts tissue differentiation. Additionally changes in Slit3 expression might contribute to cancer progression by affecting tumor angiogenesis; this interaction involves proteins such as Robo1 and Robo4 making them potential targets in disease modulation.
style
left-column
style
left-column

Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:
style
left-column

Target data

May act as molecular guidance cue in cellular migration, and function may be mediated by interaction with roundabout homolog receptors.
See full target information SLIT3
style
left-column

Publications (2)

Recent publications for all applications. Explore the full list and refine your search

International journal of molecular medicine 37:1139-45 PubMed26935869

2016

Slit-miR-218-Robo axis regulates retinal neovascularization.

Applications

Unspecified application

Species

Unspecified reactive species

Yichun Kong,Bei Sun,Quanhong Han,Shuang Han,Yuchuan Wang,Ying Chen

FASEB journal : official publication of the Federation of American Societies for Experimental Biology 26:3365-79 PubMed22611085

2012

CCN2/CTGF regulates neovessel formation via targeting structurally conserved cystine knot motifs in multiple angiogenic regulators.

Applications

WB

Species

Unspecified reactive species

Liya Pi,Anitha K Shenoy,Jianwen Liu,Seungbum Kim,Nikole Nelson,Huiming Xia,William W Hauswirth,Bryon E Petersen,Gregory S Schultz,Edward W Scott
View all publications
style
left-column
style
left-column
style
right-column

Alternative Products

Primary Antibodies

AB198726

Anti-Slit3 antibody

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Slit3 antibody (AB198726)

  • 0
  • 0

Complementary Products

Secondary Antibodies

AB150077

Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488)

Immunocytochemistry/ Immunofluorescence - Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) (AB150077)

  • 5
  • 20
Primary Antibodies

AB134166

Anti-Slit2 antibody [EPR2771]

RabMAb|Recombinant

Western blot - Anti-Slit2 antibody [EPR2771] (AB134166)

  • 0
  • 0
Primary Antibodies

AB256791

Anti-Robo1 antibody [EPR23699-159]

RabMAb|Recombinant

Western blot - Anti-Robo1 antibody [EPR23699-159] (AB256791)

  • 0
  • 0
Primary Antibodies

AB109012

Anti-SQSTM1 / p62 antibody [EPR4844] - Autophagosome Marker

KO Validated|RabMAb|Recombinant|Lab Essentials

Western blot - Anti-SQSTM1 / p62 antibody [EPR4844] - Autophagosome Marker (AB109012)

  • 5
  • 15

Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.
For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com