JavaScript is disabled in your browser. Please enable JavaScript to view this website.
AB226986

Anti-SLK antibody

Be the first to review this product! Submit a review

|

(1 Publication)

Rabbit Polyclonal SLK antibody. Suitable for WB, ICC/IF and reacts with Human samples. Cited in 1 publication. Immunogen corresponding to Recombinant Fragment Protein within Human SLK.

View Alternative Names

KIAA0204, STK2, SLK, STE20-like serine/threonine-protein kinase, STE20-like kinase, hSLK, CTCL tumor antigen se20-9, STE20-related serine/threonine-protein kinase, Serine/threonine-protein kinase 2, STE20-related kinase

3 Images
Western blot - Anti-SLK antibody (AB226986)
  • WB

Supplier Data

Western blot - Anti-SLK antibody (AB226986)

5% SDS-PAGE gel.

All lanes:

Western blot - Anti-SLK antibody (ab226986) at 1/1000 dilution

Lane 1:

A549 (human lung carcinoma cell line) cell lysate at 30 µg

Lane 2:

H1299 cell lysate at 30 µg

Predicted band size: 143 kDa

false

Immunocytochemistry/ Immunofluorescence - Anti-SLK antibody (AB226986)
  • ICC/IF

Supplier Data

Immunocytochemistry/ Immunofluorescence - Anti-SLK antibody (AB226986)

4% paraformaldehyde-fixed H1299 cells stained for SLK (green) using ab226986 at 1/500 dilution in ICC/IF.

Blue : Hoechst 33342 staining.

Western blot - Anti-SLK antibody (AB226986)
  • WB

Lab

Western blot - Anti-SLK antibody (AB226986)

All lanes:

Western blot - Anti-SLK antibody (ab226986) at 1/1000 dilution

Lane 1:

WT 293T cell extracts at 30 µg

Lane 2:

SLK Knockout 293T cell extracts at 30 µg

Secondary

All lanes:

HRP-conjugated anti-rabbit IgG

Predicted band size: 143 kDa

false

Key facts

Host species

Rabbit

Clonality

Polyclonal

Isotype

IgG

Carrier free

No

Reacts with

Human

Applications

WB, ICC/IF

applications

Immunogen

Recombinant Fragment Protein within Human SLK. The exact immunogen used to generate this antibody is proprietary information.

Q9H2G2

style
left-column

Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "ICCIF" : {"fullname" : "Immunocytochemistry/ Immunofluorescence", "shortname":"ICC/IF"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/500 - 1/3000", "WB-species-notes": "<p></p>", "ICCIF-species-checked": "testedAndGuaranteed", "ICCIF-species-dilution-info": "1/100 - 1/1000", "ICCIF-species-notes": "<p></p>" } } }
style
left-column
style
left-column

Properties and storage information

Form
Liquid
Purification technique
Affinity purification Immunogen
Storage buffer
pH: 7 Preservative: 0.025% Proclin 300 Constituents: PBS, 20% Glycerol (glycerin, glycerine)
Shipped at conditions
Blue Ice
Appropriate short-term storage duration
1-2 weeks
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
-20°C
Aliquoting information
Upon delivery aliquot
Storage information
Avoid freeze / thaw cycle
style
left-column

Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

SLK also known as Ste20-like kinase or STLK3 is a serine/threonine-protein kinase. It shows mechanical activity through phosphorylation of specific substrates facilitating cellular processes like migration and proliferation. SLK has an approximate mass of 140 kDa and predominantly occurs in various tissues including epithelial and neural tissues. Its expression is regulated by multiple factors contributing to its dynamic functional role in cells.
Biological function summary

SLK plays an essential role in regulating cytoskeletal organization and cell motility allowing rearrangement of actin fibers. It operates as part of a complex involved in signal transduction cascades that influence cellular shape and movement. This kinase modulates pathways important for cell cycle progression and apoptosis affecting cellular dynamics and integrity.

Pathways

SLK is integral to both the Rho and MAPK signaling pathways which are fundamental for cellular stress responses and growth signals. Within these pathways SLK interacts with proteins such as MINK1 and TAO1 contributing to the coordination of cellular responses to environmental changes and stress signals. SLK's role supports critical intracellular communications that decide cellular fate and adaptation processes.

SLK associates with cancer particularly in cases involving abnormal cell migration and metastasis. It interacts with proteins like the focal adhesion kinase (FAK) which strengthens its participation in oncogenic pathways. SLK also has links to neurodegenerative diseases where its deregulation affects cell survival and death pathways influencing disease progression due to its involvement with proteins like caspase-3 in apoptotic processes.
style
left-column
style
left-column

Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:
style
left-column

Target data

Mediates apoptosis and actin stress fiber dissolution.
See full target information SLK
style
left-column

Publications (1)

Recent publications for all applications. Explore the full list and refine your search

Cancer management and research 12:9417-9428 PubMed33061629

2020

MicroRNA-139 Suppresses the Tumorigenicity of Triple Negative Breast Cancer Cells by Targeting SOX8.

Applications

Unspecified application

Species

Unspecified reactive species

Liangliang Dong,Dongmei Zhou,Chunxia Xin,Bing Liu,Ping Sun
View all publications
style
left-column
style
left-column
style
right-column

Alternative Products

Primary Antibodies

AB65113

Anti-SLK antibody

Western blot - Anti-SLK antibody (AB65113)

  • 4
  • 1

Complementary Products

Primary Antibodies

AB230184

Anti-RRAGC antibody

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-RRAGC antibody (AB230184)

  • 0
  • 0
Primary Antibodies

AB76901

Anti-ZCCHC6 antibody

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ZCCHC6 antibody (AB76901)

  • 0
  • 0
Primary Antibodies

AB303565

Anti-CD68 antibody [RM1031]

BOND RX™ Validated|KO Validated|RabMAb|Recombinant|Lab Essentials|20ul selling size

Western blot - Anti-CD68 antibody [RM1031] (AB303565)

  • 0
  • 0
Primary Antibodies

AB133493

Anti-HDAC6 antibody [EPR1698(2)]

20ul selling size|RabMAb|Recombinant|KO Validated|Advanced Validation

Flow Cytometry (Intracellular) - Anti-HDAC6 antibody [EPR1698(2)] (AB133493)

  • 3
  • 1

Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.
For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com