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AB65113

Anti-SLK antibody

4

(1 Review)

|

(2 Publications)

Rabbit Polyclonal SLK antibody. Suitable for WB, IHC-P, ICC/IF and reacts with Human samples. Cited in 2 publications.

View Alternative Names

KIAA0204, STK2, SLK, STE20-like serine/threonine-protein kinase, STE20-like kinase, hSLK, CTCL tumor antigen se20-9, STE20-related serine/threonine-protein kinase, Serine/threonine-protein kinase 2, STE20-related kinase

3 Images
Immunocytochemistry/ Immunofluorescence - Anti-SLK antibody (AB65113)
  • ICC/IF

Unknown

Immunocytochemistry/ Immunofluorescence - Anti-SLK antibody (AB65113)

Immunofluorescence analysis of HepG2 cells, using ab65113 antibody at 1/500-1/1000 dilution

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SLK antibody (AB65113)
  • IHC-P

Unknown

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SLK antibody (AB65113)

This image shows human skeletal muscle tissue stained with ab65113 at 1/50 - 1/100 dilution; with and without the immunising peptide.

Western blot - Anti-SLK antibody (AB65113)
  • WB

Unknown

Western blot - Anti-SLK antibody (AB65113)

The amount of positive control loading for the WB is 5-30 ug of total protein. The amount of the peptide for the WB is 5-10 ug.

All lanes:

Western blot - Anti-SLK antibody (ab65113) at 1/500 dilution

Lane 1:

extracts from COLO205 cells

Lane 2:

extracts from COLO205 cells with immunising peptide

Predicted band size: 143 kDa

Observed band size: 143 kDa

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Key facts

Host species

Rabbit

Clonality

Polyclonal

Isotype

IgG

Carrier free

No

Reacts with

Human

Applications

ICC/IF, WB, IHC-P

applications

Immunogen

The exact immunogen used to generate this antibody is proprietary information.

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"}, "ICCIF" : {"fullname" : "Immunocytochemistry/ Immunofluorescence", "shortname":"ICC/IF"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/500 - 1/1000", "WB-species-notes": "<p></p>", "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "1/50 - 1/100", "IHCP-species-notes": "<p></p>", "ICCIF-species-checked": "testedAndGuaranteed", "ICCIF-species-dilution-info": "1/500 - 1/1000", "ICCIF-species-notes": "<p></p>" } } }
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Properties and storage information

Form
Liquid
Purification technique
Affinity purification Immunogen
Storage buffer
pH: 7.4 Preservative: 0.02% Sodium azide Constituents: PBS, 50% Glycerol (glycerin, glycerine), 0.87% Sodium chloride
Shipped at conditions
Blue Ice
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
-20°C
Storage information
Stable for 12 months at -20°C
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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

SLK also known as Ste20-like kinase or STLK3 is a serine/threonine-protein kinase. It shows mechanical activity through phosphorylation of specific substrates facilitating cellular processes like migration and proliferation. SLK has an approximate mass of 140 kDa and predominantly occurs in various tissues including epithelial and neural tissues. Its expression is regulated by multiple factors contributing to its dynamic functional role in cells.
Biological function summary

SLK plays an essential role in regulating cytoskeletal organization and cell motility allowing rearrangement of actin fibers. It operates as part of a complex involved in signal transduction cascades that influence cellular shape and movement. This kinase modulates pathways important for cell cycle progression and apoptosis affecting cellular dynamics and integrity.

Pathways

SLK is integral to both the Rho and MAPK signaling pathways which are fundamental for cellular stress responses and growth signals. Within these pathways SLK interacts with proteins such as MINK1 and TAO1 contributing to the coordination of cellular responses to environmental changes and stress signals. SLK's role supports critical intracellular communications that decide cellular fate and adaptation processes.

SLK associates with cancer particularly in cases involving abnormal cell migration and metastasis. It interacts with proteins like the focal adhesion kinase (FAK) which strengthens its participation in oncogenic pathways. SLK also has links to neurodegenerative diseases where its deregulation affects cell survival and death pathways influencing disease progression due to its involvement with proteins like caspase-3 in apoptotic processes.
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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:
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Target data

Mediates apoptosis and actin stress fiber dissolution.
See full target information SLK
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Publications (2)

Recent publications for all applications. Explore the full list and refine your search

Genome research 27:1087-1097 PubMed28420690

2017

Identification of protein features encoded by alternative exons using Exon Ontology.

Applications

ICC/IF

Species

Human

Léon-Charles Tranchevent,Fabien Aubé,Louis Dulaurier,Clara Benoit-Pilven,Amandine Rey,Arnaud Poret,Emilie Chautard,Hussein Mortada,François-Olivier Desmet,Fatima Zahra Chakrama,Maira Alejandra Moreno-Garcia,Evelyne Goillot,Stéphane Janczarski,Franck Mortreux,Cyril F Bourgeois,Didier Auboeuf

Oncogene 29:2393-403 PubMed20154719

2010

ASAP1 promotes tumor cell motility and invasiveness, stimulates metastasis formation in vivo, and correlates with poor survival in colorectal cancer patients.

Applications

WB

Species

Human

T Müller,U Stein,A Poletti,L Garzia,M Rothley,D Plaumann,W Thiele,M Bauer,A Galasso,P Schlag,M Pankratz,M Zollo,J P Sleeman
View all publications
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Product promise

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