AB302537

Anti-SLK antibody [EPR25108-252] (BSA and Azide free)

RabMAb
Recombinant
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Rabbit Recombinant Monoclonal SLK antibody. Carrier free. Suitable for WB and reacts with Human, Mouse, Rat samples.

View Alternative Names

KIAA0204, STK2, SLK, STE20-like serine/threonine-protein kinase, STE20-like kinase, hSLK, CTCL tumor antigen se20-9, STE20-related serine/threonine-protein kinase, Serine/threonine-protein kinase 2, STE20-related kinase

2 Images

Supplier Data

Western blot - Anti-SLK antibody [EPR25108-252] (BSA and Azide free) (AB302537)

This data was developed using ab302536, the same antibody clone in a different buffer formulation.

Blocking and diluting buffer and concentration : 5% NFDM/TBST.

This blot was developed using a high sensitivity ECL substrate. The high-sensitivity ECL substrate used allows for the detection of proteins in the mid-femtogram range.

Unknown bands around 140 kDa, 40 kDa and 26 kDa are observed.

All lanes:

Western blot - Anti-SLK antibody [EPR25108-252] (<a href='/en-us/products/primary-antibodies/slk-antibody-epr25108-252-ab302536'>ab302536</a>) at 1/1000 dilution

Lane 1:

HeLa (human cervical adenocarcinoma epithelial cell) transfected with scrambled siRNA control, whole cell lysate at 20 µg

Lane 2:

HeLa transfected with siRNA specifically targeting SLK, whole cell lysate at 20 µg

Secondary

All lanes:

Western blot - Goat Anti-Rabbit IgG H&L (HRP) (<a href='/en-us/products/secondary-antibodies/goat-rabbit-igg-h-l-hrp-ab97051'>ab97051</a>) at 1/100000 dilution

Observed band size: 220 kDa

true

Exposure time: 3min

Supplier Data

Western blot - Anti-SLK antibody [EPR25108-252] (BSA and Azide free) (AB302537)

This data was developed using ab302536, the same antibody clone in a different buffer formulation. Blocking and diluting buffer and concentration : 5% NFDM/TBST Lysates were freshly made and used for Western blotting immediately to minimize protein degradation. Unknown bands around 140 kDa, 40 kDa and 26 kDa are observed.

All lanes:

Western blot - Anti-SLK antibody [EPR25108-252] (<a href='/en-us/products/primary-antibodies/slk-antibody-epr25108-252-ab302536'>ab302536</a>) at 1/1000 dilution

Lane 1:

HeLa (human cervical adenocarcinoma epithelial cell), whole cell lysate 20 µg

Lane 2:

293T (human embryonic kidney epithelial cell), whole cell lysate 20 µg

Lane 3:

NIH/3T3 (mouse embryonic fibroblast), whole cell lysate 20 µg

Lane 4:

PC-12 (rat adrenal gland pheochromocytoma), whole cell lysate 20 µg

Secondary

All lanes:

Western blot - Goat Anti-Rabbit IgG H&L (HRP) (<a href='/en-us/products/secondary-antibodies/goat-rabbit-igg-h-l-hrp-ab97051'>ab97051</a>) at 1/20000 dilution

Observed band size: 220 kDa

false

Exposure time: 3min

Unconjugated

Anti-SLK antibody [EPR25108-252]

Key facts

Host species

Rabbit

Clonality

Monoclonal

Clone number

EPR25108-252

Isotype

IgG

Carrier free

Yes

Reacts with

Human, Mouse, Rat

Applications

applications

Immunogen

The exact immunogen used to generate this antibody is proprietary information.

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"}, "ICCIF" : {"fullname" : "Immunocytochemistry/ Immunofluorescence", "shortname":"ICC/IF"}, "FlowCytIntra" : {"fullname" : "Flow Cytometry (Intracellular)", "shortname":"Flow Cyt (Intra)"}, "IP" : {"fullname" : "Immunoprecipitation", "shortname":"IP"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "", "WB-species-notes": "", "IHCP-species-checked": "notRecommended", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "ICCIF-species-checked": "notRecommended", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "", "FlowCytIntra-species-checked": "notRecommended", "FlowCytIntra-species-dilution-info": "", "FlowCytIntra-species-notes": "", "IP-species-checked": "notRecommended", "IP-species-dilution-info": "", "IP-species-notes": "" }, "Mouse": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "", "WB-species-notes": "", "IHCP-species-checked": "notRecommended", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "ICCIF-species-checked": "notRecommended", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "", "FlowCytIntra-species-checked": "notRecommended", "FlowCytIntra-species-dilution-info": "", "FlowCytIntra-species-notes": "", "IP-species-checked": "notRecommended", "IP-species-dilution-info": "", "IP-species-notes": "" }, "Rat": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "", "WB-species-notes": "", "IHCP-species-checked": "notRecommended", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "ICCIF-species-checked": "notRecommended", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "", "FlowCytIntra-species-checked": "notRecommended", "FlowCytIntra-species-dilution-info": "", "FlowCytIntra-species-notes": "", "IP-species-checked": "notRecommended", "IP-species-dilution-info": "", "IP-species-notes": "" } } }

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Product details

ab302537 is a carrier-free version of ab302536.

ab302536 does not react in: IHC-P for human, mouse, and rat; ICC for human, and mouse; Flow cyt. intra. for human, and mouse; IP for human, and mouse species.

Patented technology
Our RabMAb^® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb^® patents.

What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:

- High batch-to-batch consistency and reproducibility
- Improved sensitivity and specificity
- Long-term security of supply
- Animal-free batch production

For more information, read more on recombinant antibodies.

Conjugation ready
Our carrier-free antibodies are typically supplied in a PBS-only formulation, purified and free of BSA, sodium azide and glycerol. This conjugation-ready format is designed for use with fluorochromes, metal isotopes, oligonucleotides, and enzymes, which makes them ideal for antibody labelling, functional and cell-based assays, flow-based assays (e.g. mass cytometry) and Multiplex Imaging applications.

Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with 1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.

Compatibility
This product is compatible with the Maxpar^® Antibody Labeling Kit from Fluidigm, without the need for antibody preparation. Maxpar^® is a trademark of Fluidigm Canada Inc.

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Properties and storage information

Form

Liquid

Purification technique

Affinity purification Protein A

Storage buffer

pH: 7.2 - 7.4 Constituents: PBS

Shipped at conditions

Blue Ice

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

+4°C

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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

SLK also known as Ste20-like kinase or STLK3 is a serine/threonine-protein kinase. It shows mechanical activity through phosphorylation of specific substrates facilitating cellular processes like migration and proliferation. SLK has an approximate mass of 140 kDa and predominantly occurs in various tissues including epithelial and neural tissues. Its expression is regulated by multiple factors contributing to its dynamic functional role in cells.

Biological function summary

SLK plays an essential role in regulating cytoskeletal organization and cell motility allowing rearrangement of actin fibers. It operates as part of a complex involved in signal transduction cascades that influence cellular shape and movement. This kinase modulates pathways important for cell cycle progression and apoptosis affecting cellular dynamics and integrity.

Pathways

SLK is integral to both the Rho and MAPK signaling pathways which are fundamental for cellular stress responses and growth signals. Within these pathways SLK interacts with proteins such as MINK1 and TAO1 contributing to the coordination of cellular responses to environmental changes and stress signals. SLK's role supports critical intracellular communications that decide cellular fate and adaptation processes.

SLK associates with cancer particularly in cases involving abnormal cell migration and metastasis. It interacts with proteins like the focal adhesion kinase (FAK) which strengthens its participation in oncogenic pathways. SLK also has links to neurodegenerative diseases where its deregulation affects cell survival and death pathways influencing disease progression due to its involvement with proteins like caspase-3 in apoptotic processes.

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

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Target data

Mediates apoptosis and actin stress fiber dissolution.

See full target information SLK

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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.

For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com

Anti-SLK antibody [EPR25108-252] (BSA and Azide free)

Western blot - Anti-SLK antibody [EPR25108-252] (BSA and Azide free) (AB302537)

Western blot - Anti-SLK antibody [EPR25108-252] (BSA and Azide free) (AB302537)

Related conjugates and formulations (1)

Key facts

Host species

Clonality

Clone number

Isotype

Carrier free

Reacts with

Applications

Immunogen

Complementary Products

Primary Antibodies

Primary Antibodies

Primary Antibodies

Primary Antibodies

Reactivity data

Product details

Properties and storage information

Form

Purification technique

Storage buffer

Shipped at conditions

Appropriate short-term storage conditions

Appropriate long-term storage conditions

Supplementary information

Biological function summary

Pathways

Product protocols

Target data

Product promise