Skip to main content

Rabbit Recombinant Monoclonal MYH7 antibody. Suitable for mIHC, IHC-Fr, IHC-P and reacts with Human, Mouse, Rat samples. Cited in 9 publications.


Images

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Slow Skeletal Myosin Heavy chain antibody [EPR22697-17] (AB234431), expandable thumbnail
  • Immunohistochemistry (Frozen sections) - Anti-Slow Skeletal Myosin Heavy chain antibody [EPR22697-17] (AB234431), expandable thumbnail
  • Immunohistochemistry (Frozen sections) - Anti-Slow Skeletal Myosin Heavy chain antibody [EPR22697-17] (AB234431), expandable thumbnail
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Slow Skeletal Myosin Heavy chain antibody [EPR22697-17] (AB234431), expandable thumbnail
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Slow Skeletal Myosin Heavy chain antibody [EPR22697-17] (AB234431), expandable thumbnail

Publications

Key facts

Isotype
IgG
Host species
Rabbit
Storage buffer

pH: 7.2 - 7.4
Preservative: 0.01% Sodium azide
Constituents: PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA

Form
Liquid
Clonality
Monoclonal

Immunogen

  • The exact immunogen used to generate this antibody is proprietary information.

Reactivity data

Select an application
Product promiseTestedExpectedPredictedNot recommended
mIHCIPWBICC/IFIHC-FrIHC-P
Human
Tested
Not recommended
Not recommended
Not recommended
Expected
Tested
Mouse
Tested
Not recommended
Not recommended
Not recommended
Tested
Tested
Rat
Expected
Not recommended
Not recommended
Not recommended
Expected
Tested

Tested
Tested

Species
Human
Dilution info
1/2500
Notes

Perform heat-mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

Species
Mouse
Dilution info
1/2500
Notes

-

Expected
Expected

Species
Rat
Dilution info
Use at an assay dependent concentration.
Notes

-

Not recommended
Not recommended

Species
Mouse
Dilution info
-
Notes

Perform heat-mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

Species
Rat
Dilution info
-
Notes

Perform heat-mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

Species
Human
Dilution info
-
Notes

Perform heat-mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

Not recommended
Not recommended

Species
Mouse, Rat, Human
Dilution info
-
Notes

-

Not recommended
Not recommended

Species
Mouse, Rat, Human
Dilution info
-
Notes

-

Tested
Tested

Species
Mouse
Dilution info
1/200
Notes

Heat mediated antigen retrieval using sodium citrate buffer (10mM citrate pH 6.0 + 0.05% Tween-20)

Expected
Expected

Species
Human, Rat
Dilution info
Use at an assay dependent concentration.
Notes

-

Tested
Tested

Species
Mouse
Dilution info
1/2000
Notes

Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0, epitope retrieval solution 2) for 20 mins.

Perform heat-mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

Species
Rat
Dilution info
1/2000
Notes

Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0, epitope retrieval solution 2) for 20 mins.

Perform heat-mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

Species
Human
Dilution info
1/2000
Notes

Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0, epitope retrieval solution 2) for 20 mins.

Perform heat-mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

Associated Products

Select an associated product type

2 products for Alternative Product

Target data

Function

Myosins are actin-based motor molecules with ATPase activity essential for muscle contraction. Forms regular bipolar thick filaments that, together with actin thin filaments, constitute the fundamental contractile unit of skeletal and cardiac muscle.

Alternative names

MYHCB, MYH7, Myosin-7, Myosin heavy chain 7, Myosin heavy chain slow isoform, MyHC-slow, MyHC-beta

Recommended products

Rabbit Recombinant Monoclonal MYH7 antibody. Suitable for mIHC, IHC-Fr, IHC-P and reacts with Human, Mouse, Rat samples. Cited in 9 publications.

Key facts

Isotype
IgG
Form
Liquid
Clonality
Monoclonal
Immunogen
  • The exact immunogen used to generate this antibody is proprietary information.
Clone number
EPR22697-17
Purification technique
Affinity purification Protein A
Concentration
Loading...

Storage

Shipped at conditions
Blue Ice
Appropriate short-term storage duration
1-2 weeks
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
-20°C
Aliquoting information
Upon delivery aliquot
Storage information
Avoid freeze / thaw cycle

Notes

Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

This product is a recombinant monoclonal antibody, which offers several advantages including:

  • - High batch-to-batch consistency and reproducibility
  • - Improved sensitivity and specificity
  • - Long-term security of supply
  • - Animal-free batch production

For more information, read more on recombinant antibodies.

Supplementary info

This supplementary information is collated from multiple sources and compiled automatically.
Activity summary

The slow skeletal myosin heavy chain also known as anti-slow or anti-sluggish is an essential protein component of muscle tissue. This protein is identified as MYH7 chain or type D chain with a molecular mass around 220 kDa. It is predominantly expressed in slow-twitch muscle fibers which are found in skeletal muscles responsible for enduring low-intensity (endurance) activities. Myosin staining techniques often highlight the distribution and density of these fibers in muscle tissues providing insights into fiber-type composition.

Biological function summary

This myosin heavy chain participates in muscle contraction by interacting with actin filaments forming cross-bridges through ATP-dependent mechanisms. As a part of the sarcomeric structure MYH7 works within the thick filament complex contributing to the contractile force of slow-twitch muscles. This particular myosin isoform is important for maintaining muscle stamina and efficiency over prolonged activities providing the necessary mechanical support for the generation of force at a lower contraction speed compared to fast-twitch fibers.

Pathways

The slow skeletal myosin heavy chain is integral to the calcium signaling pathway that regulates muscle contraction. This protein interacts with troponin and tropomyosin to modulate the actin-myosin interaction in a calcium-dependent manner. Additionally it plays a role in the heart muscle contraction pathway linking it to proteins such as actin which is important for cardiac function and rhythmic contraction.

Associated diseases and disorders

Slow skeletal myosin heavy chain mutations are linked to cardiomyopathy particularly hypertrophic cardiomyopathy and dilated cardiomyopathy. These conditions compromise heart muscle function leading to symptoms such as fatigue and shortness of breath. The MYH7-associated disorder is often observed alongside alterations in other sarcomeric proteins like troponin T which further disrupts muscle contraction and cardiac output. Understanding these connections can aid in developing interventions and treatments for muscle-related diseases.

Product promise

We are dedicated to supporting your work with high quality reagents and we are here for you every step of the way should you need us.

In the unlikely event of one of our products not working as expected, you are covered by our product promise.

Full details and terms and conditions can be found here:
Terms & Conditions.

9 product images

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Slow Skeletal Myosin Heavy chain antibody [EPR22697-17] (ab234431), expandable thumbnail

    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Slow Skeletal Myosin Heavy chain antibody [EPR22697-17] (ab234431)

    Immunohistochemical analysis of paraffin-embedded human skeletal muscle tissue labeling Slow Skeletal Myosin Heavy chain using ab234431 at 1/2000 dilution, followed by a ready to use Rabbit specific IHC polymer detection kit HRP/DAB (Rabbit specific IHC polymer detection kit HRP/DAB ab209101). Cytoplasmic staining on the human skeletal muscle (PMID: 22530000). The section was incubated with ab234431 for 30 mins at room temperature. The immunostaining was performed on a Leica Biosystems BOND® RX instrument. Counterstained with hematoxylin.

    Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is a ready to use Rabbit specific IHC polymer detection kit HRP/DAB (Rabbit specific IHC polymer detection kit HRP/DAB ab209101).

    Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

  • Immunohistochemistry (Frozen sections) - Anti-Slow Skeletal Myosin Heavy chain antibody [EPR22697-17] (ab234431), expandable thumbnail

    Immunohistochemistry (Frozen sections) - Anti-Slow Skeletal Myosin Heavy chain antibody [EPR22697-17] (ab234431)

    Immunohistochemical analysis of 4% paraformaldehyde-fixed 0.2% Triton X-100 permeabilized rat skeletal muscle tissue labeling Slow Skeletal Myosin Heavy chain using ab234431 at 1/2000 dilution (green), followed by an AlexaFluor®488 Goat anti-Rabbit secondary (Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) ab150077) at 1/1000 dilution. Cytoplasmic staining on the rat skeletal muscle (PMID: 29193153). The nuclear counterstain was DAPI (Blue).

    Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is an AlexaFluor®488 Goat anti-Rabbit secondary (Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) ab150077) at 1/1000 dilution.

  • Immunohistochemistry (Frozen sections) - Anti-Slow Skeletal Myosin Heavy chain antibody [EPR22697-17] (ab234431), expandable thumbnail

    Immunohistochemistry (Frozen sections) - Anti-Slow Skeletal Myosin Heavy chain antibody [EPR22697-17] (ab234431)

    Immunohistochemical analysis of 4% paraformaldehyde-fixed 0.2% Triton X-100 permeabilized mouse skeletal muscle tissue labeling Slow Skeletal Myosin Heavy chain using ab234431 at 1/2000 dilution (green), followed by an AlexaFluor®488 Goat anti-Rabbit secondary (Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) ab150077) at 1/1000 dilution. Cytoplasmic staining on the mouse skeletal muscle (PMID: 29193153). The nuclear counterstain was DAPI (Blue).

    Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is an AlexaFluor®488 Goat anti-Rabbit secondary (Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) ab150077) at 1/1000 dilution.

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Slow Skeletal Myosin Heavy chain antibody [EPR22697-17] (ab234431), expandable thumbnail

    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Slow Skeletal Myosin Heavy chain antibody [EPR22697-17] (ab234431)

    Immunohistochemical analysis of paraffin-embedded human spleen tissue labeling Slow Skeletal Myosin Heavy chain using ab234431 at 1/2000 dilution, followed by a ready to use Rabbit specific IHC polymer detection kit HRP/DAB (Rabbit specific IHC polymer detection kit HRP/DAB ab209101). Negative staining on the smooth muscle in the human spleen (PMID: 22530000). The section was incubated with ab234431 for 30 mins at room temperature. The immunostaining was performed on a Leica Biosystems BOND® RX instrument. Counterstained with hematoxylin.

    Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is a ready to use Rabbit specific IHC polymer detection kit HRP/DAB (Rabbit specific IHC polymer detection kit HRP/DAB ab209101).

    Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Slow Skeletal Myosin Heavy chain antibody [EPR22697-17] (ab234431), expandable thumbnail

    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Slow Skeletal Myosin Heavy chain antibody [EPR22697-17] (ab234431)

    Immunohistochemical analysis of paraffin-embedded rat cardiac muscle tissue labeling Slow Skeletal Myosin Heavy chain using ab234431 at 1/2000 dilution, followed by a ready to use Rabbit specific IHC polymer detection kit HRP/DAB (Rabbit specific IHC polymer detection kit HRP/DAB ab209101). Cytoplasmic staining on the rat cardiac muscle (PMID: 22530000). The section was incubated with ab234431 for 30 mins at room temperature. The immunostaining was performed on a Leica Biosystems BOND® RX instrument. Counterstained with hematoxylin.

    Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is a ready to use Rabbit specific IHC polymer detection kit HRP/DAB (Rabbit specific IHC polymer detection kit HRP/DAB ab209101).

    Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Slow Skeletal Myosin Heavy chain antibody [EPR22697-17] (ab234431), expandable thumbnail

    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Slow Skeletal Myosin Heavy chain antibody [EPR22697-17] (ab234431)

    Immunohistochemical analysis of paraffin-embedded mouse cardiac muscle tissue labeling Slow Skeletal Myosin Heavy chain using ab234431 at 1/2000 dilution, followed by a ready to use Rabbit specific IHC polymer detection kit HRP/DAB (Rabbit specific IHC polymer detection kit HRP/DAB ab209101). Cytoplasmic staining on the mouse cardiac muscle (PMID: 22530000). The section was incubated with ab234431 for 30 mins at room temperature. The immunostaining was performed on a Leica Biosystems BOND® RX instrument. Counterstained with hematoxylin.

    Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is a ready to use Rabbit specific IHC polymer detection kit HRP/DAB (Rabbit specific IHC polymer detection kit HRP/DAB ab209101).

    Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Slow Skeletal Myosin Heavy chain antibody [EPR22697-17] (ab234431), expandable thumbnail

    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Slow Skeletal Myosin Heavy chain antibody [EPR22697-17] (ab234431)

    Immunohistochemical analysis of paraffin-embedded human cardiac muscle tissue labeling Slow Skeletal Myosin Heavy chain using ab234431 at 1/2000 dilution, followed by a ready to use Rabbit specific IHC polymer detection kit HRP/DAB (Rabbit specific IHC polymer detection kit HRP/DAB ab209101). Cytoplasmic staining on the human cardiac muscle (PMID: 22530000). The section was incubated with ab234431 for 30 mins at room temperature. The immunostaining was performed on a Leica Biosystems BOND® RX instrument. Counterstained with hematoxylin.

    Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is a ready to use Rabbit specific IHC polymer detection kit HRP/DAB (Rabbit specific IHC polymer detection kit HRP/DAB ab209101).

    Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

  • Multiplex immunohistochemistry - Anti-Slow Skeletal Myosin Heavy chain antibody [EPR22697-17] (ab234431), expandable thumbnail

    Multiplex immunohistochemistry - Anti-Slow Skeletal Myosin Heavy chain antibody [EPR22697-17] (ab234431)

    Fluorescence multiplex immunohistochemical analysis of the human skeletal muscle (Formalin/PFA-fixed paraffin-embedded sections).

    Panel A: merged staining of anti-delta Sarcoglycan (Anti-delta Sarcoglycan antibody [EPR8706] ab137101, green; Opal™690), anti-Fast Myosin Skeletal Heavy chain + MYH4 (Anti-Fast Myosin Skeletal Heavy chain + MYH4 antibody [EPR22880-64] ab221149, magenta; Opal™520) and anti-Slow Skeletal Myosin Heavy chain (ab234431, red; Opal™570) on human skeletal muscle. Panel B: anti-Fast Myosin Skeletal Heavy chain + MYH4 stained on fast type fibers. Panel C: anti-Slow Skeletal Myosin Heavy chain stained on slow type fibers. Panel D: anti-delta Sarcoglycan stained on membrane of skeletal muscle. Opal Polymer HRP Ms + Rb was used as a secondary antibody.

    The section was incubated in three rounds of staining: in the order of Anti-delta Sarcoglycan antibody [EPR8706] ab137101 at 1/1000 (1.043 μg/ml), Anti-Fast Myosin Skeletal Heavy chain + MYH4 antibody [EPR22880-64] ab221149 at 1/1000 (0.505 μg/ml, and ab234431 at 1/4000 (0.255 μg/ml) for 30 mins at room temperature. Each round was followed by a separate fluorescent tyramide signal amplification system. DAPI (blue) was used as a nuclear counter stain.

    The immunostaining was performed on a Leica Biosystems BOND® RX instrument with an Opal™ 4-color kit. Image acquisition was performed with Leica SP8 confocal microscope.

    Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0, epitope retrieval solution2) for 20 mins.

  • Multiplex immunohistochemistry - Anti-Slow Skeletal Myosin Heavy chain antibody [EPR22697-17] (ab234431), expandable thumbnail

    Multiplex immunohistochemistry - Anti-Slow Skeletal Myosin Heavy chain antibody [EPR22697-17] (ab234431)

    Multiplex immunohistochemistry analysis of formalin/PFA-fixed paraffin-embedded Mouse skeletal muscle tissue staining Slow Skeletal Myosin Heavy chain with ab234431 at a 1/2500 dilution, A2BP1 with Anti-A2BP1/Fox1/RBFOX1 antibody [EPR23656-5] ab254413 at 1/2000 (0.26 µg/ml) dilution and Dystrophin with Anti-Dystrophin antibody [EPR21189] ab218198 at 1/5000 ( 0.110 µg/ml) dilution.

    Panel A: merged staining of anti-Slow Skeletal Myosin Heavy chain (green; Opal™520), anti-A2BP1 (gray; Opal™570) and anti-Dystrophin (magenta; Opal™690) on mouse skeletal muscle.
    Panel B: anti-Slow Skeletal Myosin Heavy chain staining slow type fibers in mouse skeletal muscle.
    Panel C: anti-A2BP1 staining nucleus in mouse skeletal muscle.
    Panel D: anti-Dystrophin staining membrane in mouse skeletal muscle.
    Nuclear DNA was labeled with DAPI (shown in blue).

    The section was incubated in three rounds of staining: in the order of ab234431, Anti-A2BP1/Fox1/RBFOX1 antibody [EPR23656-5] ab254413 and Anti-Dystrophin antibody [EPR21189] ab218198 for 30 mins at room temperature. Each round was followed by a separate fluorescent tyramide signal amplification system.

    The immunostaining was performed on a Leica Biosystems BOND® RX instrument with an Opal™ 4-color kit. Image acquisition was performed with Leica SP8 confocal microscope.

    Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0 epitope retrieval solution2) for 20 mins.

Downloads

Product protocols

For this product, it's our understanding that no specific protocols are required. You can:

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com