Anti-Slow Skeletal Myosin Heavy chain antibody [EPR22697-17] - BSA and Azide free

• IHC-P

Unknown

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Slow Skeletal Myosin Heavy chain antibody [EPR22697-17] - BSA and Azide free (AB255278)

Immunohistochemical analysis of paraffin-embedded human skeletal muscle tissue labeling Slow Skeletal Myosin Heavy chain using ab234431 at 1/2000 dilution, followed by a ready to use Rabbit specific IHC polymer detection kit HRP/DAB (ab209101). Cytoplasmic staining on the human skeletal muscle (PMID : 22530000). The section was incubated with ab234431 for 30 mins at room temperature. The immunostaining was performed on a Leica Biosystems BOND^® RX instrument. Counterstained with hematoxylin.

Secondary antibody only control : Used PBS instead of primary antibody, secondary antibody is a ready to use Rabbit specific IHC polymer detection kit HRP/DAB (ab209101).

This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab234431).

Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

• IHC-P

Unknown

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Slow Skeletal Myosin Heavy chain antibody [EPR22697-17] - BSA and Azide free (AB255278)

Immunohistochemical analysis of paraffin-embedded human spleen tissue labeling Slow Skeletal Myosin Heavy chain using ab234431 at 1/2000 dilution, followed by a ready to use Rabbit specific IHC polymer detection kit HRP/DAB (ab209101). Negative staining on the smooth muscle in the human spleen (PMID : 22530000). The section was incubated with ab234431 for 30 mins at room temperature. The immunostaining was performed on a Leica Biosystems BOND^® RX instrument. Counterstained with hematoxylin.

Secondary antibody only control : Used PBS instead of primary antibody, secondary antibody is a ready to use Rabbit specific IHC polymer detection kit HRP/DAB (ab209101).

This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab234431).

Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

• mIHC

Supplier Data

Multiplex immunohistochemistry - Anti-Slow Skeletal Myosin Heavy chain antibody [EPR22697-17] - BSA and Azide free (AB255278)

Fluorescence multiplex immunohistochemical analysis of the human skeletal muscle (Formalin/PFA-fixed paraffin-embedded sections). Panel A : merged staining of anti-delta Sarcoglycan (ab137101, green; Opal™690), anti-Fast Myosin Skeletal Heavy chain + MYH4 (ab221149, magenta; Opal™520) and anti-Slow Skeletal Myosin Heavy chain (ab234431, red; Opal™570) on human skeletal muscle. Panel B : anti-Fast Myosin Skeletal Heavy chain + MYH4 stained on fast type fibers. Panel C : anti-Slow Skeletal Myosin Heavy chain stained on slow type fibers. Panel D : anti-delta Sarcoglycan stained on membrane of skeletal muscle. Opal Polymer HRP Ms + Rb was used as a secondary antibody. The section was incubated in three rounds of staining : in the order of ab137101 at 1/1000 (1.043 μg/ml), ab221149 at 1/1000 (0.505 μg/ml, and ab234431 at 1/4000 (0.255 μg/ml) for 30 mins at room temperature. Each round was followed by a separate fluorescent tyramide signal amplification system. DAPI (blue) was used as a nuclear counter stain. The immunostaining was performed on a Leica Biosystems BOND^® RX instrument with an Opal™ 4-color kit. Image acquisition was performed with Leica SP8 confocal microscope. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0, epitope retrieval solution2) for 20 mins. This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab234431).

• IHC-P

Unknown

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Slow Skeletal Myosin Heavy chain antibody [EPR22697-17] - BSA and Azide free (AB255278)

Immunohistochemical analysis of paraffin-embedded human cardiac muscle tissue labeling Slow Skeletal Myosin Heavy chain using ab234431 at 1/2000 dilution, followed by a ready to use Rabbit specific IHC polymer detection kit HRP/DAB (ab209101). Cytoplasmic staining on the human cardiac muscle (PMID : 22530000). The section was incubated with ab234431 for 30 mins at room temperature. The immunostaining was performed on a Leica Biosystems BOND^® RX instrument. Counterstained with hematoxylin.

Secondary antibody only control : Used PBS instead of primary antibody, secondary antibody is a ready to use Rabbit specific IHC polymer detection kit HRP/DAB (ab209101).

This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab234431).

Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

• IHC-Fr

Unknown

Immunohistochemistry (Frozen sections) - Anti-Slow Skeletal Myosin Heavy chain antibody [EPR22697-17] - BSA and Azide free (AB255278)

Immunohistochemical analysis of 4% paraformaldehyde-fixed 0.2% Triton X-100 permeabilized mouse skeletal muscle tissue labeling Slow Skeletal Myosin Heavy chain using ab234431 at 1/2000 dilution (green), followed by an AlexaFluor^®488 Goat anti-Rabbit secondary (ab150077) at 1/1000 dilution. Cytoplasmic staining on the mouse skeletal muscle (PMID : 29193153). The nuclear counterstain was DAPI (Blue).

Secondary antibody only control : Used PBS instead of primary antibody, secondary antibody is an AlexaFluor^®488 Goat anti-Rabbit secondary (ab150077) at 1/1000 dilution.

This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab234431).

• mIHC

Lab

Multiplex immunohistochemistry - Anti-Slow Skeletal Myosin Heavy chain antibody [EPR22697-17] - BSA and Azide free (AB255278)

This data was developed using ab234431 the same antibody clone in a different buffer formulation.

Multiplex immunohistochemistry analysis of formalin/PFA-fixed paraffin-embedded Mouse skeletal muscle tissue staining Slow Skeletal Myosin Heavy chain with ab234431 at a 1/2500 dilution, A2BP1 with ab254413 at 1/2000 (0.26 µg/ml) dilution and Dystrophin with ab218198 at 1/5000 ( 0.110 µg/ml) dilution.

Panel A : merged staining of anti-Slow Skeletal Myosin Heavy chain (green; Opal™520), anti-A2BP1 (gray; Opal™570) and anti-Dystrophin (magenta; Opal™690) on mouse skeletal muscle.
Panel B : anti-Slow Skeletal Myosin Heavy chain staining slow type fibers in mouse skeletal muscle.
Panel C : anti-A2BP1 staining nucleus in mouse skeletal muscle.
Panel D : anti-Dystrophin staining membrane in mouse skeletal muscle.
Nuclear DNA was labeled with DAPI (shown in blue).

The section was incubated in three rounds of staining : in the order of ab234431, ab254413 and ab218198 for 30 mins at room temperature. Each round was followed by a separate fluorescent tyramide signal amplification system.

The immunostaining was performed on a Leica Biosystems BOND^® RX instrument with an Opal™ 4-color kit. Image acquisition was performed with Leica SP8 confocal microscope.

Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0 epitope retrieval solution2) for 20 mins.

• IHC-P

Unknown

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Slow Skeletal Myosin Heavy chain antibody [EPR22697-17] - BSA and Azide free (AB255278)

Immunohistochemical analysis of paraffin-embedded rat cardiac muscle tissue labeling Slow Skeletal Myosin Heavy chain using ab234431 at 1/2000 dilution, followed by a ready to use Rabbit specific IHC polymer detection kit HRP/DAB (ab209101). Cytoplasmic staining on the rat cardiac muscle (PMID : 22530000). The section was incubated with ab234431 for 30 mins at room temperature. The immunostaining was performed on a Leica Biosystems BOND^® RX instrument. Counterstained with hematoxylin.

Secondary antibody only control : Used PBS instead of primary antibody, secondary antibody is a ready to use Rabbit specific IHC polymer detection kit HRP/DAB (ab209101).

This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab234431).

Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

• IHC-Fr

Unknown

Immunohistochemistry (Frozen sections) - Anti-Slow Skeletal Myosin Heavy chain antibody [EPR22697-17] - BSA and Azide free (AB255278)

Immunohistochemical analysis of 4% paraformaldehyde-fixed 0.2% Triton X-100 permeabilized rat skeletal muscle tissue labeling Slow Skeletal Myosin Heavy chain using ab234431 at 1/2000 dilution (green), followed by an AlexaFluor^®488 Goat anti-Rabbit secondary (ab150077) at 1/1000 dilution. Cytoplasmic staining on the rat skeletal muscle (PMID : 29193153). The nuclear counterstain was DAPI (Blue).

Secondary antibody only control : Used PBS instead of primary antibody, secondary antibody is an AlexaFluor^®488 Goat anti-Rabbit secondary (ab150077) at 1/1000 dilution.

This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab234431).

• IHC-P

Unknown

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Slow Skeletal Myosin Heavy chain antibody [EPR22697-17] - BSA and Azide free (AB255278)

Immunohistochemical analysis of paraffin-embedded mouse cardiac muscle tissue labeling Slow Skeletal Myosin Heavy chain using ab234431 at 1/2000 dilution, followed by a ready to use Rabbit specific IHC polymer detection kit HRP/DAB (ab209101). Cytoplasmic staining on the mouse cardiac muscle (PMID : 22530000). The section was incubated with ab234431 for 30 mins at room temperature. The immunostaining was performed on a Leica Biosystems BOND^® RX instrument. Counterstained with hematoxylin.

Secondary antibody only control : Used PBS instead of primary antibody, secondary antibody is a ready to use Rabbit specific IHC polymer detection kit HRP/DAB (ab209101).

This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab234431).

Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.