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AB228353

Anti-Slow Skeletal Myosin Heavy chain antibody - N-terminal

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(2 Publications)

Rabbit Polyclonal MYH7 antibody. N-terminal. Suitable for WB and reacts with Mouse samples. Cited in 2 publications. Immunogen corresponding to Synthetic Peptide within Human MYH7.

View Alternative Names

MYHCB, MYH7, Myosin-7, Myosin heavy chain 7, Myosin heavy chain slow isoform, MyHC-slow, MyHC-beta

1 Images
Western blot - Anti-Slow Skeletal Myosin Heavy chain antibody - N-terminal (AB228353)
  • WB

Supplier Data

Western blot - Anti-Slow Skeletal Myosin Heavy chain antibody - N-terminal (AB228353)

5% SDS-PAGE

All lanes:

Western blot - Anti-Slow Skeletal Myosin Heavy chain antibody - N-terminal (ab228353) at 1/2000 dilution

All lanes:

Mouse muscle tissue extract at 50 µg

Predicted band size: 223 kDa

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Key facts

Host species

Rabbit

Clonality

Polyclonal

Isotype

IgG

Carrier free

No

Reacts with

Mouse

Applications

WB

applications

Immunogen

Synthetic Peptide within Human MYH7. The exact immunogen used to generate this antibody is proprietary information.

P12883

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Primary Antibodies

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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Myosin heavy chain antibody [A4.1025] (AB37484)

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Primary Antibodies

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Secondary Antibodies

AB150113

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Immunocytochemistry/ Immunofluorescence - Goat Anti-Mouse IgG H&L (Alexa Fluor® 488) (AB150113)

  • 5
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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "" }, "Mouse": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/500 - 1/3000", "WB-species-notes": "<p></p>" } } }
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Properties and storage information

Form
Liquid
Purification technique
Affinity purification Immunogen
Storage buffer
pH: 7 Preservative: 0.01% Thimerosal (merthiolate) Constituents: 10% Glycerol (glycerin, glycerine), 1.21% Tris, 0.75% Glycine
Shipped at conditions
Blue Ice
Appropriate short-term storage duration
1-2 weeks
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
-20°C
Aliquoting information
Upon delivery aliquot
Storage information
Avoid freeze / thaw cycle
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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

The slow skeletal myosin heavy chain also known as anti-slow or anti-sluggish is an essential protein component of muscle tissue. This protein is identified as MYH7 chain or type D chain with a molecular mass around 220 kDa. It is predominantly expressed in slow-twitch muscle fibers which are found in skeletal muscles responsible for enduring low-intensity (endurance) activities. Myosin staining techniques often highlight the distribution and density of these fibers in muscle tissues providing insights into fiber-type composition.
Biological function summary

This myosin heavy chain participates in muscle contraction by interacting with actin filaments forming cross-bridges through ATP-dependent mechanisms. As a part of the sarcomeric structure MYH7 works within the thick filament complex contributing to the contractile force of slow-twitch muscles. This particular myosin isoform is important for maintaining muscle stamina and efficiency over prolonged activities providing the necessary mechanical support for the generation of force at a lower contraction speed compared to fast-twitch fibers.

Pathways

The slow skeletal myosin heavy chain is integral to the calcium signaling pathway that regulates muscle contraction. This protein interacts with troponin and tropomyosin to modulate the actin-myosin interaction in a calcium-dependent manner. Additionally it plays a role in the heart muscle contraction pathway linking it to proteins such as actin which is important for cardiac function and rhythmic contraction.

Slow skeletal myosin heavy chain mutations are linked to cardiomyopathy particularly hypertrophic cardiomyopathy and dilated cardiomyopathy. These conditions compromise heart muscle function leading to symptoms such as fatigue and shortness of breath. The MYH7-associated disorder is often observed alongside alterations in other sarcomeric proteins like troponin T which further disrupts muscle contraction and cardiac output. Understanding these connections can aid in developing interventions and treatments for muscle-related diseases.
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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:
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Target data

Myosins are actin-based motor molecules with ATPase activity essential for muscle contraction. Forms regular bipolar thick filaments that, together with actin thin filaments, constitute the fundamental contractile unit of skeletal and cardiac muscle.
See full target information MYH7
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Publications (2)

Recent publications for all applications. Explore the full list and refine your search

NPJ microgravity 10:18 PubMed38365862

2024

Simulated microgravity attenuates myogenesis and contractile function of 3D engineered skeletal muscle tissues.

Applications

Unspecified application

Species

Unspecified reactive species

Zhanping Ren,Eun Hyun Ahn,Minjae Do,Devin B Mair,Amir Monemianesfahani,Peter H U Lee,Deok-Ho Kim

Heliyon 9:e14383 PubMed36938474

2023

Cigarette smoke preferentially induces full length ACE2 expression in differentiated primary human airway cultures but does not alter the efficiency of cellular SARS-CoV-2 infection.

Applications

Unspecified application

Species

Unspecified reactive species

Linsey M Porter,Wenrui Guo,Thomas Wm Crozier,Edward Jd Greenwood,Brian Ortmann,Daniel Kottmann,James A Nathan,Ravindra Mahadeva,Paul J Lehner,Frank McCaughan
View all publications
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Product promise

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