AB11083

Anti-Slow Skeletal Myosin Heavy chain antibody [NOQ7.5.4D]

Name: Anti-Slow Skeletal Myosin Heavy chain antibody [NOQ7.5.4D] (ab11083) | Abcam
Brand: Abcam Limited
SKU: AB11083
Rating: 5 (14 reviews)

(14 Reviews)

(144 Publications)

Anti-Slow Skeletal Myosin Heavy chain antibody [NOQ7.5.4D] (ab11083) is a mouse monoclonal antibody detecting Slow Skeletal Myosin Heavy chain in Western Blot, IHC-P. Suitable for Rabbit, Rat.

- Over 110 publications
- Trusted since 2004

View Alternative Names

MYHCB, MYH7, Myosin-7, Myosin heavy chain 7, Myosin heavy chain slow isoform, MyHC-slow, MyHC-beta

4 Images

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Slow Skeletal Myosin Heavy chain antibody [NOQ7.5.4D] (AB11083)

IHC-P

AbReview45619****

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Slow Skeletal Myosin Heavy chain antibody [NOQ7.5.4D] (AB11083)

ab11083 at 1/500 dilution, staining Slow Skeletal Myosin Heavy chain in mouse tissue sections by Immunohistochemistry (Formalin/PFA-fixed paraffin embedded sections).

Supplier Data

Western blot - Anti-Slow Skeletal Myosin Heavy chain antibody [NOQ7.5.4D] (AB11083)

Lane 1:

Western blot - Anti-Slow Skeletal Myosin Heavy chain antibody [NOQ7.5.4D] (ab11083) at 1.25 µg/mL

Lane 2:

Western blot - Anti-Slow Skeletal Myosin Heavy chain antibody [NOQ7.5.4D] (ab11083) at 2.5 µg/mL

Lane 3:

Negative control antibody

All lanes:

Rat skeletal muscle

Secondary

All lanes:

Anti-Mouse IgG (Fac-specific)-peroxidase conjugate at 1/5000 dilution

Predicted band size: 223 kDa

false

IHC-P

Unknown

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Slow Skeletal Myosin Heavy chain antibody [NOQ7.5.4D] (AB11083)

ab11083 at 1/4000 dilution, staining Slow Skeletal Myosin Heavy chain in rabbit tongue tissue section by Immunohistochemistry (Formalin/PFA-fixed paraffin embedded sections).

IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Slow Skeletal Myosin Heavy chain antibody [NOQ7.5.4D] (AB11083)

Immunohistochemical analysis of formalin-fixed, paraffin-embedded rabbit tomgue tissue staining Skeletal Myosin Heavy chain using ab11083 at 2.5 μg/mL.

Key facts

Host species

Mouse

Clonality

Monoclonal

Clone number

NOQ7.5.4D

Isotype

IgG1

Carrier free

Reacts with

Rat, Rabbit

Applications

IHC-P, WB

applications

Immunogen

Native Full Length Protein corresponding to Human MYH7.

P12883

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Reactivity data

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Product details

What is this antibody validated in?
Anti-Slow Skeletal Myosin Heavy chain antibody [NOQ7.5.4D] (ab11083) is a mouse monoclonal antibody and is validated for use in Western Blot (WB), Immunohistochemistry (IHC-P) in Rabbit, Rat samples.

What is the molecular weight of Slow Skeletal Myosin Heavy chain?
Anti-Slow Skeletal Myosin Heavy chain [NOQ7.5.4D] (ab11083) specifically detects a band for Slow Skeletal Myosin Heavy chain (UniProt: P12883) at a molecular weight of 200kDa.

Trusted by the scientific community
Anti-Slow Skeletal Myosin Heavy chain [NOQ7.5.4D] (ab11083) was first used in a scientific publication in 2004 and has been cited over 110 times in peer-reviewed journals.

Reviewed by scientists
Anti-Slow Skeletal Myosin Heavy chain [NOQ7.5.4D] (ab11083) has over 10 independent reviews from customers.

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Properties and storage information

Form

Liquid

Purification technique

Proprietary technique

Purification notes

Purified from Tissue culture supernatant.

Storage buffer

pH: 7.4 Preservative: 0.097% Sodium azide Constituents: PBS

Shipped at conditions

Blue Ice

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

-20°C

Aliquoting information

Upon delivery aliquot

Storage information

Avoid freeze / thaw cycle

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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

The slow skeletal myosin heavy chain also known as anti-slow or anti-sluggish is an essential protein component of muscle tissue. This protein is identified as MYH7 chain or type D chain with a molecular mass around 220 kDa. It is predominantly expressed in slow-twitch muscle fibers which are found in skeletal muscles responsible for enduring low-intensity (endurance) activities. Myosin staining techniques often highlight the distribution and density of these fibers in muscle tissues providing insights into fiber-type composition.

Biological function summary

This myosin heavy chain participates in muscle contraction by interacting with actin filaments forming cross-bridges through ATP-dependent mechanisms. As a part of the sarcomeric structure MYH7 works within the thick filament complex contributing to the contractile force of slow-twitch muscles. This particular myosin isoform is important for maintaining muscle stamina and efficiency over prolonged activities providing the necessary mechanical support for the generation of force at a lower contraction speed compared to fast-twitch fibers.

Pathways

The slow skeletal myosin heavy chain is integral to the calcium signaling pathway that regulates muscle contraction. This protein interacts with troponin and tropomyosin to modulate the actin-myosin interaction in a calcium-dependent manner. Additionally it plays a role in the heart muscle contraction pathway linking it to proteins such as actin which is important for cardiac function and rhythmic contraction.

Slow skeletal myosin heavy chain mutations are linked to cardiomyopathy particularly hypertrophic cardiomyopathy and dilated cardiomyopathy. These conditions compromise heart muscle function leading to symptoms such as fatigue and shortness of breath. The MYH7-associated disorder is often observed alongside alterations in other sarcomeric proteins like troponin T which further disrupts muscle contraction and cardiac output. Understanding these connections can aid in developing interventions and treatments for muscle-related diseases.

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

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Target data

Myosins are actin-based motor molecules with ATPase activity essential for muscle contraction. Forms regular bipolar thick filaments that, together with actin thin filaments, constitute the fundamental contractile unit of skeletal and cardiac muscle.

See full target information MYH7

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Publications (144)

Recent publications for all applications. Explore the full list and refine your search

Frontiers in cell and developmental biology 13:1649640 PubMed40959669

2025

Decoding the prenatal clock of sheep muscle fiber type differentiation: a temporal map from embryonic to mature types.

Applications

Unspecified application

Species

Unspecified reactive species

Zhenzhen Gu,Weiwei Duan,Chenxi Liu,Wenrong Li,Ning Zhang,Bin Han,Mingjun Liu

Frontiers in veterinary science 12:1637277 PubMed40808770

2025

miRNA-182-5p promotes myogenic differentiation of C2C12 cells via the suppression of ZBTB7A.

Applications

Unspecified application

Species

Unspecified reactive species

Mengyuan Zhang,Yongheng Wang,Shan Shan,Siyu Liu

Journal of animal science and biotechnology 16:93 PubMed40598440

2025

Co-cultivation of Lactobacillus acidophilus and Bacillus subtilis mediates the gut-muscle axis affecting pork quality and flavor.

Applications

Unspecified application

Species

Unspecified reactive species

Zhixin Lin,Xinchen Zhou,Tingting Lu,Wendong An,Shenghao Chen,Suchen Li,Hui Miao,Xinyan Han

Stem cell research & therapy 16:336 PubMed40597262

2025

VEGF-B-mediated myofiber types involved in high-fat diet-induced hyperglycemia through PKA-NFATs signaling pathway.

Applications

Unspecified application

Species

Unspecified reactive species

Liu-Liu Shi,Yu-Ting Sun,Jia-Nan Sun,Jing Yue,Wei Chen,Kun Meng,Long Chen,Chang-Qing Hu,Rui Chen,Dong-Sheng Sun,Cheng-Bao Xu,Wei Yuan,Xin-Li Li,Dan Zhao,Yan Wu,Shi-Bing Xi,Xiao-Ying Zhao,Jun-Ming Tang

Poultry science 104:105419 PubMed40516297

2025

Developmental characteristics of skeletal muscle in goose during the embryonic and early post-hatching periods.

Applications

Unspecified application

Species

Unspecified reactive species

Kaiqi Weng,Yi Liu,Huiying Wang,Guohong Chen,Daqian He,Qi Xu

Animal nutrition (Zhongguo xu mu shou yi xue hui) 21:207-221 PubMed40487094

2025

Fibroblast growth factor 21/extracellular signal-regulated kinase 1/2 pathway as a key link mediates the effects of low-protein diet on skeletal muscle fiber type transformation in pigs.

Applications

Unspecified application

Species

Unspecified reactive species

Shuo Li,Pengbo Liang,Bo Wang,Jun Chen,Jinming You,Tiande Zou

Skeletal muscle 15:9 PubMed40128889

2025

Endurance exercise with reduced muscle glycogen content influences substrate utilization and attenuates acute mTORC1- and autophagic signaling in human type I and type II muscle fibers.

Applications

Unspecified application

Species

Unspecified reactive species

Oscar Horwath,Lucas Cornet,Henrik Strömlind,Marcus Moberg,Sebastian Edman,Karin Söderlund,Antonio Checa,Jorge L Ruas,Eva Blomstrand

Scientific reports 15:7384 PubMed40025125

2025

Remodeling of the cardiac striatin interactome and its dynamics in the diabetic heart.

Applications

Unspecified application

Species

Unspecified reactive species

Stephanie Chacar,Wael Abdrabou,Cynthia Al Hageh,Liaqat Ali,Thenmozhi Venkatachalam,Pierre Zalloua,M-Saadeh Suleiman,Frank Christopher Howarth,Ali A Khraibi,Moni Nader

Animals : an open access journal from MDPI 15: PubMed39943074

2025

Effects of Free-Range Systems on Muscle Fiber Characteristics and Welfare Indicators in Geese.

Applications

Unspecified application

Species

Unspecified reactive species

Guoyao Wang,Jianzhou Chen,Yujiao Guo,Kaiqi Weng,Yu Zhang,Yang Zhang,Guohong Chen,Qi Xu,Yang Chen

Journal of cachexia, sarcopenia and muscle 16:e13693 PubMed39901373

2025

Nicotinamide Phosphoribosyltransferase Acetylation Mediating Muscle Dysfunction Contributes to Sleep Apnoea in Obesity.

Applications

Unspecified application

Species

Unspecified reactive species

Liu Zhang,Ya Ru Yan,Shi Qi Li,Ying Ni Lin,Yi Wang,Yu Qing Wang,Ning Li,Fang Ying Lu,Xian Wen Sun,Li Yue Zhang,Jian Ping Zhou,Yong Jie Ding,Qing Yun Li

View all publications

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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.

For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com

Anti-Slow Skeletal Myosin Heavy chain antibody [NOQ7.5.4D]

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Slow Skeletal Myosin Heavy chain antibody [NOQ7.5.4D] (AB11083)

Western blot - Anti-Slow Skeletal Myosin Heavy chain antibody [NOQ7.5.4D] (AB11083)

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Slow Skeletal Myosin Heavy chain antibody [NOQ7.5.4D] (AB11083)

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Slow Skeletal Myosin Heavy chain antibody [NOQ7.5.4D] (AB11083)

Key facts

Host species

Clonality

Clone number

Isotype

Carrier free

Reacts with

Applications

Immunogen

Alternative Products

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Primary Antibodies

Primary Antibodies

Complementary Products

Primary Antibodies

Secondary Antibodies

Primary Antibodies

Primary Antibodies

Reactivity data

Product details

Properties and storage information

Form

Purification technique

Purification notes

Storage buffer

Shipped at conditions

Appropriate short-term storage conditions

Appropriate long-term storage conditions

Aliquoting information

Storage information

Supplementary information

Biological function summary

Pathways

Product protocols

Target data

Publications (144)

Product promise