AB89025

Anti-SLP-2 antibody

Name: Anti-SLP-2 antibody (ab89025) | Abcam
Brand: Abcam Limited
SKU: AB89025
Price: 545 USD
Availability: InStock

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Mouse Polyclonal SLP-2 antibody. Carrier free. Suitable for WB and reacts with Human samples. Immunogen corresponding to Recombinant Full Length Protein corresponding to Human STOML2.

View Alternative Names

SLP2, HSPC108, STOML2, SLP-2, EPB72-like protein 2, Paraprotein target 7, Paratarg-7

4 Images

Lab

Western blot - Anti-SLP-2 antibody (AB89025)

Western blot : Anti-SLP-2 antibody ab89025 staining at 1 µg/mL, shown in green; Rabbit anti alpha Tubulin (ab52866) loading control staining at 1/20,000 dilution, shown in magenta. A band was observed at 40 kDa in Wild-type A549 cell lysates with no signal observed at this size in STOML2 knockout A549 cell line.

To generate this image, samples were run on an SDS-PAGE gel then transferred onto a nitrocellulose membrane. Membranes were blocked in 3pc Milk in TBS-0.1 % Tween^® 20 (TBS-T) before incubation with primary antibodies overnight at 4 °C. Blots were washed four times in TBS-T, incubated with secondary antibodies for 1 h at room temperature, washed again four times then imaged. Secondary antibodies used were Goat anti-Mouse 800CW & Goat anti-Rabbit 680RD at 1/20,000 dilution.

All lanes:

Western blot - Anti-SLP-2 antibody (ab89025) at 1 µg/mL

Lane 1:

Wild-type A549 at 20 µg

Lane 2:

STOML2 knockout A549 at 20 µg

Lane 3:

HeLa at 20 µg

Lane 4:

PC-3 at 20 µg

Secondary

All lanes:

Goat anti-Mouse 800CW & Goat anti-Rabbit 680RD at 1/20000 dilution

Predicted band size: 40 kDa

Observed band size: 40 kDa

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Western blot - Anti-SLP-2 antibody (AB89025)

All lanes:

Western blot - Anti-SLP-2 antibody (ab89025) at 1 µg/mL

All lanes:

A431 cell lysate at 50 µg

Predicted band size: 39 kDa

Observed band size: 39 kDa

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Unknown

Western blot - Anti-SLP-2 antibody (AB89025)

All lanes:

Western blot - Anti-SLP-2 antibody (ab89025) at 1 µg/mL

All lanes:

human placenta tissue lysate at 50 µg

Predicted band size: 39 kDa

Observed band size: 39 kDa

false

Unknown

Western blot - Anti-SLP-2 antibody (AB89025)

All lanes:

Western blot - Anti-SLP-2 antibody (ab89025) at 1 µg/mL

Lane 1:

SLP-2 transfected 293T cell lysate at 25 µg

Lane 2:

Non transfected 293T lysate at 25 µg

Predicted band size: 39 kDa

Observed band size: 39 kDa

false

Key facts

Host species

Mouse

Clonality

Polyclonal

Isotype

IgG

Carrier free

Yes

Reacts with

Human

Applications

applications

Immunogen

Recombinant Full Length Protein corresponding to Human STOML2.

Q9UJZ1

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1 µg/mL", "WB-species-notes": "<p></p>" } } }

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Properties and storage information

Form

Liquid

Purification technique

Affinity purification Protein A

Storage buffer

pH: 7.4 Constituents: 100% PBS

Shipped at conditions

Blue Ice

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

-20°C

Aliquoting information

Upon delivery aliquot

Storage information

Avoid freeze / thaw cycle

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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

SLP-2 also known as stomatin-like protein 2 is a protein with a mass of approximately 38 kDa. It is widely expressed in different tissues with high presence in mitochondrial inner membrane domains. This protein belongs to the stomatin protein family and possesses functions related to cellular compartmentalization and stability.

Biological function summary

Stomatin-like protein 2 is integral to maintaining mitochondrial morphology and function. It is closely involved in regulating mitochondrial membrane potential and apoptosis. As part of a multiprotein complex SLP-2 helps in organizing lipid and protein microdomains which are important for various mitochondrial processes and cellular energy dynamics.

Pathways

Stomatin-like protein 2 contributes significantly to the oxidative phosphorylation and apoptosis pathways. It interacts with proteins such as prohibitins which are critical in maintaining mitochondria integrity and function. These interactions highlight SLP-2's role in both energy production and programmed cell death highlighting its significance in cellular metabolic regulation and stress response.

Stomatin-like protein 2 has been linked to cancer and cardiovascular disorders. Its up-regulation is often observed in various cancers suggesting a role in tumorigenesis by affecting the cellular energy balance and mitochondrial dynamics. The protein also connects to cardiolipin a lipid implicated in heart diseases illustrating its involvement in cardiovascular conditions through mitochondrial dysfunction.

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:

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Target data

Mitochondrial protein that probably regulates the biogenesis and the activity of mitochondria. Stimulates cardiolipin biosynthesis, binds cardiolipin-enriched membranes where it recruits and stabilizes some proteins including prohibitin and may therefore act in the organization of functional microdomains in mitochondrial membranes. Through regulation of the mitochondrial function may play a role into several biological processes including cell migration, cell proliferation, T-cell activation, calcium homeostasis and cellular response to stress. May play a role in calcium homeostasis through negative regulation of calcium efflux from mitochondria. Required for mitochondrial hyperfusion a pro-survival cellular response to stress which results in increased ATP production by mitochondria. May also regulate the organization of functional domains at the plasma membrane and play a role in T-cell activation through association with the T-cell receptor signaling complex and its regulation.

See full target information STOML2

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Product promise

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For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

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Anti-SLP-2 antibody

Western blot - Anti-SLP-2 antibody (AB89025)

Western blot - Anti-SLP-2 antibody (AB89025)

Western blot - Anti-SLP-2 antibody (AB89025)

Western blot - Anti-SLP-2 antibody (AB89025)

Key facts

Host species

Clonality

Isotype

Carrier free

Reacts with

Applications

Immunogen

Reactivity data

Properties and storage information

Form

Purification technique

Storage buffer

Shipped at conditions

Appropriate short-term storage conditions

Appropriate long-term storage conditions

Aliquoting information

Storage information

Supplementary information

Biological function summary

Pathways

Product protocols

Target data

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