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Rabbit Recombinant Monoclonal SLP-2 antibody. Suitable for IP, WB, ICC/IF, Flow Cyt (Intra) and reacts with Human, Mouse, Rat samples. Cited in 1 publication.

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Images

Western blot - Anti-SLP-2 antibody [EPR18718] (AB191883), expandable thumbnail
  • Western blot - Anti-SLP-2 antibody [EPR18718] (AB191883), expandable thumbnail
  • Western blot - Anti-SLP-2 antibody [EPR18718] (AB191883), expandable thumbnail
  • Immunocytochemistry/ Immunofluorescence - Anti-SLP-2 antibody [EPR18718] (AB191883), expandable thumbnail
  • Immunocytochemistry/ Immunofluorescence - Anti-SLP-2 antibody [EPR18718] (AB191883), expandable thumbnail

Publications

Key facts

Isotype
IgG
Host species
Rabbit
Storage buffer

pH: 7.2 - 7.4
Preservative: 0.01% Sodium azide
Constituents: 59% PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA

Form
Liquid
Clonality
Monoclonal

Immunogen

  • The exact immunogen used to generate this antibody is proprietary information.

Reactivity data

Select an application
Product promiseTestedExpectedPredictedNot recommended
IPWBICC/IFFlow Cyt (Intra)
Human
Tested
Tested
Tested
Tested
Mouse
Expected
Tested
Expected
Expected
Rat
Expected
Tested
Expected
Expected

Tested
Tested

Species
Human
Dilution info
1/50
Notes

-

Expected
Expected

Species
Mouse, Rat
Dilution info
Use at an assay dependent concentration.
Notes

-

Tested
Tested

Species
Mouse
Dilution info
1/1000
Notes

-

Species
Rat
Dilution info
1/1000
Notes

-

Species
Human
Dilution info
1/1000
Notes

-

Tested
Tested

Species
Human
Dilution info
1/250
Notes

-

Expected
Expected

Species
Mouse, Rat
Dilution info
Use at an assay dependent concentration.
Notes

-

Tested
Tested

Species
Human
Dilution info
1/120
Notes

-

Expected
Expected

Species
Mouse, Rat
Dilution info
Use at an assay dependent concentration.
Notes

-

Associated Products

Select an associated product type

2 products for Alternative Product

Target data

Function

Mitochondrial protein that probably regulates the biogenesis and the activity of mitochondria. Stimulates cardiolipin biosynthesis, binds cardiolipin-enriched membranes where it recruits and stabilizes some proteins including prohibitin and may therefore act in the organization of functional microdomains in mitochondrial membranes. Through regulation of the mitochondrial function may play a role into several biological processes including cell migration, cell proliferation, T-cell activation, calcium homeostasis and cellular response to stress. May play a role in calcium homeostasis through negative regulation of calcium efflux from mitochondria. Required for mitochondrial hyperfusion a pro-survival cellular response to stress which results in increased ATP production by mitochondria. May also regulate the organization of functional domains at the plasma membrane and play a role in T-cell activation through association with the T-cell receptor signaling complex and its regulation.

Alternative names

SLP2, HSPC108, STOML2, SLP-2, EPB72-like protein 2, Paraprotein target 7, Paratarg-7

Recommended products

Rabbit Recombinant Monoclonal SLP-2 antibody. Suitable for IP, WB, ICC/IF, Flow Cyt (Intra) and reacts with Human, Mouse, Rat samples. Cited in 1 publication.

Key facts

Isotype
IgG
Form
Liquid
Clonality
Monoclonal
Immunogen
  • The exact immunogen used to generate this antibody is proprietary information.
Clone number
EPR18718
Purification technique
Affinity purification Protein A
Concentration
Loading...

Storage

Shipped at conditions
Blue Ice
Appropriate short-term storage duration
1-2 weeks
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
-20°C
Aliquoting information
Upon delivery aliquot
Storage information
Avoid freeze / thaw cycle

Notes

Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

This product is a recombinant monoclonal antibody, which offers several advantages including:

  • - High batch-to-batch consistency and reproducibility
  • - Improved sensitivity and specificity
  • - Long-term security of supply
  • - Animal-free batch production

For more information, read more on recombinant antibodies.

Supplementary info

This supplementary information is collated from multiple sources and compiled automatically.
Activity summary

SLP-2 also known as stomatin-like protein 2 is a protein with a mass of approximately 38 kDa. It is widely expressed in different tissues with high presence in mitochondrial inner membrane domains. This protein belongs to the stomatin protein family and possesses functions related to cellular compartmentalization and stability.

Biological function summary

Stomatin-like protein 2 is integral to maintaining mitochondrial morphology and function. It is closely involved in regulating mitochondrial membrane potential and apoptosis. As part of a multiprotein complex SLP-2 helps in organizing lipid and protein microdomains which are important for various mitochondrial processes and cellular energy dynamics.

Pathways

Stomatin-like protein 2 contributes significantly to the oxidative phosphorylation and apoptosis pathways. It interacts with proteins such as prohibitins which are critical in maintaining mitochondria integrity and function. These interactions highlight SLP-2's role in both energy production and programmed cell death highlighting its significance in cellular metabolic regulation and stress response.

Associated diseases and disorders

Stomatin-like protein 2 has been linked to cancer and cardiovascular disorders. Its up-regulation is often observed in various cancers suggesting a role in tumorigenesis by affecting the cellular energy balance and mitochondrial dynamics. The protein also connects to cardiolipin a lipid implicated in heart diseases illustrating its involvement in cardiovascular conditions through mitochondrial dysfunction.

Product promise

We are dedicated to supporting your work with high quality reagents and we are here for you every step of the way should you need us.

In the unlikely event of one of our products not working as expected, you are covered by our product promise.

Full details and terms and conditions can be found here:
Terms & Conditions.

8 product images

  • Western blot - Anti-SLP-2 antibody [EPR18718] (ab191883), expandable thumbnail

    Western blot - Anti-SLP-2 antibody [EPR18718] (ab191883)

    Blocking/dilution buffer: 5% NFDM/TBST.

    All lanes: Western blot - Anti-SLP-2 antibody [EPR18718] (ab191883) at 1/1000 dilution

    All lanes: Human fetal kidney lysate at 10 µg

    Secondary

    All lanes: Goat Anti-Rabbit IgG Peroxidase Conjugate, specific to the non-reduced form of IgG at 1/10000 dilution

    Predicted band size: 39 kDa

    Exposure time: 1min

  • Western blot - Anti-SLP-2 antibody [EPR18718] (ab191883), expandable thumbnail

    Western blot - Anti-SLP-2 antibody [EPR18718] (ab191883)

    Blocking/dilution buffer: 5% NFDM/TBST.

    All lanes: Western blot - Anti-SLP-2 antibody [EPR18718] (ab191883) at 1/1000 dilution

    Lane 1: HeLa (Human epithelial cell line from cervix adenocarcinoma) whole cell lysate at 20 µg

    Lane 2: Jurkat (Human T cell leukemia cell line from peripheral blood) whole cell lysate at 20 µg

    Lane 3: MCF7 (Human breast adenocarcinoma cell line) whole cell lysate at 20 µg

    Lane 4: A549 (Human lung carcinoma cell line) whole cell lysate at 20 µg

    Secondary

    All lanes: Western blot - Goat Anti-Rabbit IgG H&L (HRP) (Goat Anti-Rabbit IgG H&L (HRP) ab97051) at 1/100000 dilution

    Predicted band size: 39 kDa

    Observed band size: 39 kDa

    Exposure time: 30s

  • Western blot - Anti-SLP-2 antibody [EPR18718] (ab191883), expandable thumbnail

    Western blot - Anti-SLP-2 antibody [EPR18718] (ab191883)

    Blocking/dilution buffer: 5% NFDM/TBST.

    Exposure time: Lane1,2,3 and 4:3 minutes;Lane 5 and 6:1 minute.

    All lanes: Western blot - Anti-SLP-2 antibody [EPR18718] (ab191883) at 1/1000 dilution

    Lane 1: Mouse brain lysate at 10 µg

    Lane 2: Mouse spleen lysate at 10 µg

    Lane 3: C6 (Rat glial tumor cell line) whole cell lysate at 10 µg

    Lane 4: RAW 264.7 (Mouse macrophage cell line transformed with Abelson murine leukemia virus) whole cell lysate at 10 µg

    Lane 5: Rat brain lysate at 10 µg

    Lane 6: NIH/3T3 (Mouse embryonic fibroblast cell line) whole cell lysate at 10 µg

    Secondary

    All lanes: Western blot - Goat Anti-Rabbit IgG H&L (HRP) (Goat Anti-Rabbit IgG H&L (HRP) ab97051) at 1/100000 dilution

    Predicted band size: 227 kDa, 39 kDa

    Observed band size: 39 kDa

  • Immunocytochemistry/ Immunofluorescence - Anti-SLP-2 antibody [EPR18718] (ab191883), expandable thumbnail

    Immunocytochemistry/ Immunofluorescence - Anti-SLP-2 antibody [EPR18718] (ab191883)

    Immunofluorescence staining of HeLa cells with ab191883 at a working dilution of 1 in 250, counter-stained with DAPI. The secondary antibody was Alexa Fluor® 488 goat anti-rabbit (Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) ab150077), used at a dilution of 1 in 1000 (shown in green). The mitochondria were stained with Anti-COX IV antibody [mAbcam33985] - Mitochondrial Marker ab33985 (mouse monoclonal to COX-IV, mitochondrial marker) at 1/1000 and Goat Anti-Mouse IgG H&L (Alexa Fluor® 594) preadsorbed ab150120 at 1/1000 (shown in red). The cells were fixed in 100% methanol and permeabilized using 0.1% triton-X 100. The negative controls are shown in bottom panels. For negative control 1, ab191883 was used at a dilution of 1/250 followed by an Alexa Fluor® 594 goat anti-mouse antibody (Goat Anti-Mouse IgG H&L (Alexa Fluor® 594) preadsorbed ab150120) at a dilution of 1/1000. For negative control 2, Anti-alpha Tubulin antibody [DM1A] - Loading Control ab7291 (mouse monoclonal to alpha tubulin) was used at a dilution of 1/1000 followed by Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) ab150077 (Alexa Fluor® 488 goat anti-rabbit) at a dilution of 1/1000.

  • Immunocytochemistry/ Immunofluorescence - Anti-SLP-2 antibody [EPR18718] (ab191883), expandable thumbnail

    Immunocytochemistry/ Immunofluorescence - Anti-SLP-2 antibody [EPR18718] (ab191883)

    Immunofluorescent analysis of 100% methanol-fixed, 0.1% Triton X-100 permeabilized HeLa (Human epithelial cell line from cervix adenocarcinoma) cells labeling SLP-2 with ab191883 at 1/250 dilution, followed by Goat Anti-Rabbit IgG (Alexa Fluor® 488) (Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) ab150077) secondary antibody at 1/1000 dilution (green). Confocal image showing cytoplasmic staining on HeLa cell line. The nuclear counterstain is DAPI (blue). Tubulin is detected with Anti-alpha Tubulin -Loading Control (Anti-alpha Tubulin antibody [DM1A] - Loading Control ab7291) at 1/1000 dilution and Goat Anti-Mouse IgG (AlexaFluor®594) preadsorbed (Goat Anti-Mouse IgG H&L (Alexa Fluor® 594) preadsorbed ab150120) at 1/1000 dilution (red).
    The negative controls are as follows:-
    -ve control 1: ab191883 at 1/250 dilution followed by Goat Anti-Mouse IgG H&L (Alexa Fluor® 594) preadsorbed ab150120 at 1/1000 dilution.
    -ve control 2: Anti-alpha Tubulin antibody [DM1A] - Loading Control ab7291 at 1/1000 dilution followed by Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) ab150077 at 1/1000 dilution.

  • Immunoprecipitation - Anti-SLP-2 antibody [EPR18718] (ab191883), expandable thumbnail

    Immunoprecipitation - Anti-SLP-2 antibody [EPR18718] (ab191883)

    SLP-2 was immunoprecipitated from 1mg of Jurkat (Human T cell leukemia cell line from peripheral blood) whole cell lysate with ab191883 at 1/50 dilution. Western blot was performed from the immunoprecipitate using ab191883 at 1/1000 dilution. Veriblot for IP (HRP) (ab13136) was used as secondary antibody at 1/10000 dilution.
    Lane 1: Jurkat whole cell lysate 10μg (Input).
    Lane 2: ab191883 IP in Jurkat whole cell lysate.
    Lane 3: Rabbit IgG,monoclonal [EPR25A] (Rabbit IgG, monoclonal [EPR25A] - Isotype Control ab172730) instead of ab191883 in Jurkat whole cell cell lysate.
    Blocking and dilution buffer and concentration: 5% NFDM/TBST.
    Exposure time: 30 seconds.

    All lanes: Immunoprecipitation - Anti-SLP-2 antibody [EPR18718] (ab191883)

    Predicted band size: 39 kDa

  • Immunocytochemistry/ Immunofluorescence - Anti-SLP-2 antibody [EPR18718] (ab191883), expandable thumbnail

    Immunocytochemistry/ Immunofluorescence - Anti-SLP-2 antibody [EPR18718] (ab191883)

    Immunofluorescent analysis of 100% methanol-fixed, 0.1% Triton X-100 permeabilized Jurkat cells (Human T cell leukemia cell line from peripheral blood) labeling SLP-2 with ab191883 at 1/250 dilution, followed by Goat Anti-Rabbit IgG (Alexa Fluor® 488) (Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) ab150077) secondary antibody at 1/1000 dilution (green). Confocal image showing cytoplasmic staining on Jurkat cell line. The nuclear counterstain is DAPI (blue). Tubulin is detected with Anti-alpha Tubulin -Loading Control (Anti-alpha Tubulin antibody [DM1A] - Loading Control ab7291) at 1/1000 dilution and Goat Anti-Mouse IgG (AlexaFluor®594) preadsorbed (Goat Anti-Mouse IgG H&L (Alexa Fluor® 594) preadsorbed ab150120) at 1/1000 dilution (red).
    The negative controls are as follows:-
    -ve control 1: ab191883 at 1/250 dilution followed by Goat Anti-Mouse IgG H&L (Alexa Fluor® 594) preadsorbed ab150120 at 1/1000 dilution.
    -ve control 2: Anti-alpha Tubulin antibody [DM1A] - Loading Control ab7291 at 1/1000 dilution followed by Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) ab150077 at 1/1000 dilution.

  • Flow Cytometry (Intracellular) - Anti-SLP-2 antibody [EPR18718] (ab191883), expandable thumbnail

    Flow Cytometry (Intracellular) - Anti-SLP-2 antibody [EPR18718] (ab191883)

    Intracellular flow cytometric analysis of 4% paraformaldehyde-fixed HeLa (Human epithelial cell line from cervix adenocarcinoma) cells labeling SLP-2 with ab191883 at 1/120 dilution (red) compared with a Rabbit IgG,monoclonal [EPR25A]-Isotype control (Rabbit IgG, monoclonal [EPR25A] - Isotype Control ab172730) (black) and an unlabelled control (cells without incubation with primary antibody and secondary antibody) (blue). Goat anti Rabbit IgG (Alexa Fluor® 488) at 1/500 dilution was used as the secondary antibody.

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