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AB247808

Anti-SLP76 antibody [EPR2549(2)] - BSA and Azide free

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Rabbit Recombinant Monoclonal SLP76 antibody. Carrier free. Suitable for IHC-P, WB and reacts with Human samples.

View Alternative Names

Lymphocyte cytosolic protein 2, SH2 domain-containing leukocyte protein of 76 kDa, SLP-76 tyrosine phosphoprotein, SLP76, LCP2

6 Images
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SLP76 antibody [EPR2549(2)] - BSA and Azide free (AB247808)
  • IHC-P

Unknown

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SLP76 antibody [EPR2549(2)] - BSA and Azide free (AB247808)

This data was developed using ab109254, the same antibody clone in a different buffer formulation.

ab109254 showing positive staining in Normal human spleen tissue.

Perform heat mediated antigen retrieval before commencing with IHC staining protocol.

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SLP76 antibody [EPR2549(2)] - BSA and Azide free (AB247808)
  • IHC-P

Unknown

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SLP76 antibody [EPR2549(2)] - BSA and Azide free (AB247808)

This data was developed using ab109254, the same antibody clone in a different buffer formulation.

ab109254 showing negative staining in Normal human heart tissue.

Perform heat mediated antigen retrieval before commencing with IHC staining protocol.

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SLP76 antibody [EPR2549(2)] - BSA and Azide free (AB247808)
  • IHC-P

Unknown

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SLP76 antibody [EPR2549(2)] - BSA and Azide free (AB247808)

This data was developed using ab109254, the same antibody clone in a different buffer formulation.

ab109254 showing negative staining in Normal human brain tissue.

Perform heat mediated antigen retrieval before commencing with IHC staining protocol.

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SLP76 antibody [EPR2549(2)] - BSA and Azide free (AB247808)
  • IHC-P

Unknown

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SLP76 antibody [EPR2549(2)] - BSA and Azide free (AB247808)

This data was developed using ab109254, the same antibody clone in a different buffer formulation.

ab109254 showing positive staining in Normal human colon tissue.

Perform heat mediated antigen retrieval before commencing with IHC staining protocol.

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SLP76 antibody [EPR2549(2)] - BSA and Azide free (AB247808)
  • IHC-P

Unknown

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SLP76 antibody [EPR2549(2)] - BSA and Azide free (AB247808)

This data was developed using ab109254, the same antibody clone in a different buffer formulation.

ab109254 at 1/100 dilution, staining SLP76 in Human tonsil, by Immunohistochemistry, Paraffin-embedded tissue.

Perform heat mediated antigen retrieval before commencing with IHC staining protocol.

Western blot - Anti-SLP76 antibody [EPR2549(2)] - BSA and Azide free (AB247808)
  • WB

Unknown

Western blot - Anti-SLP76 antibody [EPR2549(2)] - BSA and Azide free (AB247808)

This data was developed using ab109254, the same antibody clone in a different buffer formulation.

All lanes:

Western blot - Anti-SLP76 antibody [EPR2549(2)] (<a href='/en-us/products/primary-antibodies/slp76-antibody-epr25492-ab109254'>ab109254</a>) at 1/1000 dilution

Lane 1:

HuT-78 cell lysate at 10 µg

Lane 2:

MOLT-4 cell lysate at 10 µg

Predicted band size: 60 kDa

false

Key facts

Host species

Rabbit

Clonality

Monoclonal

Clone number

EPR2549(2)

Isotype

IgG

Carrier free

Yes

Reacts with

Human

Applications

WB, IHC-P

applications

Immunogen

The exact immunogen used to generate this antibody is proprietary information.

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"}, "IP" : {"fullname" : "Immunoprecipitation", "shortname":"IP"}, "FlowCyt" : {"fullname" : "Flow Cytometry", "shortname":"Flow Cyt"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "", "IHCP-species-notes": "<p></p>", "IP-species-checked": "notRecommended", "IP-species-dilution-info": "", "IP-species-notes": "<p></p>", "FlowCyt-species-checked": "notRecommended", "FlowCyt-species-dilution-info": "", "FlowCyt-species-notes": "<p></p>", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "", "WB-species-notes": "<p></p>" }, "Mouse": { "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "IP-species-checked": "notRecommended", "IP-species-dilution-info": "", "IP-species-notes": "", "FlowCyt-species-checked": "notRecommended", "FlowCyt-species-dilution-info": "", "FlowCyt-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "" }, "Rat": { "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "IP-species-checked": "notRecommended", "IP-species-dilution-info": "", "IP-species-notes": "", "FlowCyt-species-checked": "notRecommended", "FlowCyt-species-dilution-info": "", "FlowCyt-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "" } } }
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Product details

ab247808 is the carrier-free version of ab109254.

Patented technology
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:

  • - High batch-to-batch consistency and reproducibility
  • - Improved sensitivity and specificity
  • - Long-term security of supply
  • - Animal-free batch production

For more information, read more on recombinant antibodies.

Conjugation ready
Our carrier-free antibodies are typically supplied in a PBS-only formulation, purified and free of BSA, sodium azide and glycerol. This conjugation-ready format is designed for use with fluorochromes, metal isotopes, oligonucleotides, and enzymes, which makes them ideal for antibody labelling, functional and cell-based assays, flow-based assays (e.g. mass cytometry) and Multiplex Imaging applications.

Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with 1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.

Compatibility
This product is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm, without the need for antibody preparation. Maxpar® is a trademark of Fluidigm Canada Inc.

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Properties and storage information

Form
Liquid
Purification technique
Affinity purification Protein A
Storage buffer
pH: 7.2 - 7.4 Constituents: PBS
Shipped at conditions
Blue Ice
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
+4°C
Storage information
Do Not Freeze
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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

SLP76 also known as Src homology 2 domain-containing leukocyte phosphoprotein of 76 kDa plays a pivotal role in intracellular signaling. This adaptor protein weighs approximately 76 kilodaltons and is predominantly expressed in hematopoietic cells particularly T cells NK cells mast cells and platelets. SLP76 functions to relay signals from the cell surface to the nucleus affecting cellular responses including activation proliferation and differentiation.
Biological function summary

This adaptor protein serves as a scaffold linking membrane-proximal signaling to downstream cellular effectors. SLP76 forms part of a larger signaling complex with proteins such as LAT (Linker for Activation of T cells) and Gads facilitating the assembly of multi-protein complexes necessary for signal transduction. It coordinates the recruitment and activation of several enzymes and substrates which leads to robust immune cell responses.

Pathways

The function of SLP76 integrates into T cell receptor (TCR) signaling and integrin signaling pathways. In TCR signaling SLP76 interacts closely with proteins LAT and ZAP-70 which are triggered upon antigen recognition. This interaction propagates the immune response by activating downstream effectors like MAP kinases and NF-kB. In integrin signaling SLP76 helps mediate cytoskeletal rearrangements which are important for cell migration and adhesion.

Mutations or dysregulation of SLP76 associate with immunodeficiencies and hematologic malignancies. Aberrant SLP76 activity can lead to inappropriate activation or inactivation of immune responses contributing to disorders as SCID (Severe Combined Immunodeficiency) and some types of leukemia. Additionally it connects with proteins such as LAT in disease contexts reflecting a shared pathway disruption contributing to the pathophysiology of these conditions.
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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:
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Target data

Adapter protein primarily involved in signaling pathways within T-cells, as well as other immune cells such as platelets, mast cells, and natural killer (NK) cells (PubMed : 11313406, PubMed : 33159816). Plays a crucial role for transducing signal from the T-cell receptor (TCR) after antigen recognition leading to T-cell activation. Mechanistically, once phosphorylated by the kinase ZAP70, mediates interactions with the guanine-nucleotide exchange factor VAV1, the adapter protein NCK and the kinase ITK (PubMed : 8673706, PubMed : 8702662). In turn, stimulates the activation of PKC-theta/PRKCQ and NF-kappa-B transcriptional activity in response to CD3 and CD28 costimulation (PubMed : 11313406). Also plays an essential role in AGER-induced signaling pathways including p38 MAPK and ERK1/2 activation leading to cytokine release and pro-inflammatory responses (PubMed : 33436632).
See full target information LCP2
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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.
For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

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