Rabbit Recombinant Monoclonal SLP76 antibody. Carrier free. Suitable for IHC-P, WB and reacts with Human samples.
Images
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SLP76 antibody [EPR2549(2)] - BSA and Azide free (AB247808), expandable thumbnail](https://content.abcam.com/products/images/slp76-antibody-epr25492-bsa-and-azide-free-ab247808--immunohistochemistry-formalin-pfa-fixed-paraffin-embedded-sections-img100101.jpg/_jcr_content/renditions/webp-475.webp "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SLP76 antibody [EPR2549(2)] - BSA and Azide free (AB247808)")
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SLP76 antibody [EPR2549(2)] - BSA and Azide free (AB247808), expandable thumbnail](https://content.abcam.com/products/images/slp76-antibody-epr25492-bsa-and-azide-free-ab247808--immunohistochemistry-formalin-pfa-fixed-paraffin-embedded-sections-img115010.jpg/_jcr_content/renditions/webp-475.webp "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SLP76 antibody [EPR2549(2)] - BSA and Azide free (AB247808)")
![Western blot - Anti-SLP76 antibody [EPR2549(2)] - BSA and Azide free (AB247808), expandable thumbnail](https://content.abcam.com/products/images/slp76-antibody-epr25492-bsa-and-azide-free-ab247808--western-blot-img147701.jpg/_jcr_content/renditions/webp-475.webp "Western blot - Anti-SLP76 antibody [EPR2549(2)] - BSA and Azide free (AB247808)")
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SLP76 antibody [EPR2549(2)] - BSA and Azide free (AB247808), expandable thumbnail](https://content.abcam.com/products/images/slp76-antibody-epr25492-bsa-and-azide-free-ab247808--immunohistochemistry-formalin-pfa-fixed-paraffin-embedded-sections-img36784.jpg/_jcr_content/renditions/webp-475.webp "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SLP76 antibody [EPR2549(2)] - BSA and Azide free (AB247808)")
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SLP76 antibody [EPR2549(2)] - BSA and Azide free (AB247808), expandable thumbnail](https://content.abcam.com/products/images/slp76-antibody-epr25492-bsa-and-azide-free-ab247808--immunohistochemistry-formalin-pfa-fixed-paraffin-embedded-sections-img133420.jpg/_jcr_content/renditions/webp-475.webp "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SLP76 antibody [EPR2549(2)] - BSA and Azide free (AB247808)")
Key facts
- Isotype
- IgG
- Host species
- Rabbit
- Storage buffer
pH: 7.2 - 7.4
Constituents: PBS- Form
- Liquid
- Clonality
- Monoclonal
Immunogen
- The exact immunogen used to generate this antibody is proprietary information.
Reactivity data
Select an application| IHC-P | IP | Flow Cyt | WB | |
|---|---|---|---|---|
| Human | Tested | Not recommended | Not recommended | Tested |
| Mouse | Predicted | Not recommended | Not recommended | Predicted |
| Rat | Predicted | Not recommended | Not recommended | Predicted |
IHC-P
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info - | Notes Perform heat-mediated antigen retrieval before commencing with IHC staining protocol. |
PredictedPredicted
| Species | Dilution info | Notes |
|---|---|---|
Species Mouse, Rat | Dilution info - | Notes - |
IP
Not recommendedNot recommended
| Species | Dilution info | Notes |
|---|---|---|
Species Human, Mouse, Rat | Dilution info - | Notes - |
Flow Cyt
Not recommendedNot recommended
| Species | Dilution info | Notes |
|---|---|---|
Species Human, Mouse, Rat | Dilution info - | Notes - |
WB
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info - | Notes - |
PredictedPredicted
| Species | Dilution info | Notes |
|---|---|---|
Species Mouse, Rat | Dilution info - | Notes - |
Target data
Function
Adapter protein primarily involved in signaling pathways within T-cells, as well as other immune cells such as platelets, mast cells, and natural killer (NK) cells (PubMed:11313406, PubMed:33159816). Plays a crucial role for transducing signal from the T-cell receptor (TCR) after antigen recognition leading to T-cell activation. Mechanistically, once phosphorylated by the kinase ZAP70, mediates interactions with the guanine-nucleotide exchange factor VAV1, the adapter protein NCK and the kinase ITK (PubMed:8673706, PubMed:8702662). In turn, stimulates the activation of PKC-theta/PRKCQ and NF-kappa-B transcriptional activity in response to CD3 and CD28 costimulation (PubMed:11313406). Plays also an essential role in AGER-induced signaling pathways including p38 MAPK and ERK1/2 activation leading to cytokine release and pro-inflammatory responses (PubMed:33436632).
Alternative names
Lymphocyte cytosolic protein 2, SH2 domain-containing leukocyte protein of 76 kDa, SLP-76 tyrosine phosphoprotein, SLP76, LCP2
Recommended products
Rabbit Recombinant Monoclonal SLP76 antibody. Carrier free. Suitable for IHC-P, WB and reacts with Human samples.
Key facts
- Isotype
- IgG
- Host species
- Rabbit
- Storage buffer
pH: 7.2 - 7.4
Constituents: PBS- Form
- Liquid
- Clonality
- Monoclonal
- Immunogen
- The exact immunogen used to generate this antibody is proprietary information.
- Carrier free
- Yes
- Clone number
- EPR2549(2)
- Purification technique
- Affinity purification Protein A
- Concentration
Storage
- Shipped at conditions
- Blue Ice
- Appropriate short-term storage conditions
- +4°C
- Appropriate long-term storage conditions
- +4°C
- Storage information
- Do Not Freeze
Notes
ab247808 is the carrier-free version of Anti-SLP76 antibody [EPR2549(2)] ab109254.
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free batch production
For more information, read more on recombinant antibodies.
Our carrier-free antibodies are typically supplied in a PBS-only formulation, purified and free of BSA, sodium azide and glycerol. The carrier-free buffer and high concentration allow for increased conjugation efficiency.
This conjugation-ready format is designed for use with fluorochromes, metal isotopes, oligonucleotides, and enzymes, which makes them ideal for antibody labelling, functional and cell-based assays, flow-based assays (e.g. mass cytometry) and Multiplex Imaging applications.
Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with 1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.
This product is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm, without the need for antibody preparation. Maxpar® is a trademark of Fluidigm Canada Inc.
Supplementary info
- This supplementary information is collated from multiple sources and compiled automatically.
- Activity summary
SLP76 also known as Src homology 2 domain-containing leukocyte phosphoprotein of 76 kDa plays a pivotal role in intracellular signaling. This adaptor protein weighs approximately 76 kilodaltons and is predominantly expressed in hematopoietic cells particularly T cells NK cells mast cells and platelets. SLP76 functions to relay signals from the cell surface to the nucleus affecting cellular responses including activation proliferation and differentiation.
- Biological function summary
This adaptor protein serves as a scaffold linking membrane-proximal signaling to downstream cellular effectors. SLP76 forms part of a larger signaling complex with proteins such as LAT (Linker for Activation of T cells) and Gads facilitating the assembly of multi-protein complexes necessary for signal transduction. It coordinates the recruitment and activation of several enzymes and substrates which leads to robust immune cell responses.
- Pathways
The function of SLP76 integrates into T cell receptor (TCR) signaling and integrin signaling pathways. In TCR signaling SLP76 interacts closely with proteins LAT and ZAP-70 which are triggered upon antigen recognition. This interaction propagates the immune response by activating downstream effectors like MAP kinases and NF-kB. In integrin signaling SLP76 helps mediate cytoskeletal rearrangements which are important for cell migration and adhesion.
- Associated diseases and disorders
Mutations or dysregulation of SLP76 associate with immunodeficiencies and hematologic malignancies. Aberrant SLP76 activity can lead to inappropriate activation or inactivation of immune responses contributing to disorders as SCID (Severe Combined Immunodeficiency) and some types of leukemia. Additionally it connects with proteins such as LAT in disease contexts reflecting a shared pathway disruption contributing to the pathophysiology of these conditions.
Product promise
Tested
We have tested this species and application combination and it works. It is covered by our product promise.
Expected
We have not tested this specific species and application combination in-house, but expect it will work. It is covered by our product promise.
Predicted
This species and application combination has not been tested, but we predict it will work based on strong homology. However, this combination is not covered by our product promise.
Not recommended
We do not recommend this combination. It is not covered by our product promise.
We are dedicated to supporting your work with high quality reagents and we are here for you every step of the way should you need us.
In the unlikely event of one of our products not working as expected, you are covered by our product promise.
Full details and terms and conditions can be found here:
Terms & Conditions.
6 product images
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SLP76 antibody [EPR2549(2)] - BSA and Azide free (ab247808), expandable thumbnail](https://content.abcam.com/products/images/slp76-antibody-epr25492-bsa-and-azide-free-ab247808--immunohistochemistry-formalin-pfa-fixed-paraffin-embedded-sections-img100101.jpg "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SLP76 antibody [EPR2549(2)] - BSA and Azide free (ab247808)")
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SLP76 antibody [EPR2549(2)] - BSA and Azide free (ab247808)
This data was developed using Anti-SLP76 antibody [EPR2549(2)] ab109254, the same antibody clone in a different buffer formulation.Anti-SLP76 antibody [EPR2549(2)] ab109254 at 1/100 dilution, staining SLP76 in Human tonsil, by Immunohistochemistry, Paraffin-embedded tissue. Perform heat mediated antigen retrieval before commencing with IHC staining protocol.
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SLP76 antibody [EPR2549(2)] - BSA and Azide free (ab247808), expandable thumbnail](https://content.abcam.com/products/images/slp76-antibody-epr25492-bsa-and-azide-free-ab247808--immunohistochemistry-formalin-pfa-fixed-paraffin-embedded-sections-img115010.jpg "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SLP76 antibody [EPR2549(2)] - BSA and Azide free (ab247808)")
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SLP76 antibody [EPR2549(2)] - BSA and Azide free (ab247808)
This data was developed using Anti-SLP76 antibody [EPR2549(2)] ab109254, the same antibody clone in a different buffer formulation.Anti-SLP76 antibody [EPR2549(2)] ab109254 showing positive staining in Normal human spleen tissue.
Perform heat mediated antigen retrieval before commencing with IHC staining protocol.![Western blot - Anti-SLP76 antibody [EPR2549(2)] - BSA and Azide free (ab247808), expandable thumbnail](https://content.abcam.com/products/images/slp76-antibody-epr25492-bsa-and-azide-free-ab247808--western-blot-img147701.jpg "Western blot - Anti-SLP76 antibody [EPR2549(2)] - BSA and Azide free (ab247808)")
Western blot - Anti-SLP76 antibody [EPR2549(2)] - BSA and Azide free (ab247808)
This data was developed using Anti-SLP76 antibody [EPR2549(2)] ab109254, the same antibody clone in a different buffer formulation.
All lanes: Western blot - Anti-SLP76 antibody [EPR2549(2)] (Anti-SLP76 antibody [EPR2549(2)] ab109254) at 1/1000 dilution
Lane 1: HuT-78 cell lysate at 10 µg
Lane 2: MOLT-4 cell lysate at 10 µg
Predicted band size: 60 kDa
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SLP76 antibody [EPR2549(2)] - BSA and Azide free (ab247808), expandable thumbnail](https://content.abcam.com/products/images/slp76-antibody-epr25492-bsa-and-azide-free-ab247808--immunohistochemistry-formalin-pfa-fixed-paraffin-embedded-sections-img36784.jpg "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SLP76 antibody [EPR2549(2)] - BSA and Azide free (ab247808)")
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SLP76 antibody [EPR2549(2)] - BSA and Azide free (ab247808)
This data was developed using Anti-SLP76 antibody [EPR2549(2)] ab109254, the same antibody clone in a different buffer formulation.Anti-SLP76 antibody [EPR2549(2)] ab109254 showing positive staining in Normal human colon tissue.
Perform heat mediated antigen retrieval before commencing with IHC staining protocol.![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SLP76 antibody [EPR2549(2)] - BSA and Azide free (ab247808), expandable thumbnail](https://content.abcam.com/products/images/slp76-antibody-epr25492-bsa-and-azide-free-ab247808--immunohistochemistry-formalin-pfa-fixed-paraffin-embedded-sections-img133420.jpg "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SLP76 antibody [EPR2549(2)] - BSA and Azide free (ab247808)")
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SLP76 antibody [EPR2549(2)] - BSA and Azide free (ab247808)
This data was developed using Anti-SLP76 antibody [EPR2549(2)] ab109254, the same antibody clone in a different buffer formulation.Anti-SLP76 antibody [EPR2549(2)] ab109254 showing negative staining in Normal human brain tissue.
Perform heat mediated antigen retrieval before commencing with IHC staining protocol.![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SLP76 antibody [EPR2549(2)] - BSA and Azide free (ab247808), expandable thumbnail](https://content.abcam.com/products/images/slp76-antibody-epr25492-bsa-and-azide-free-ab247808--immunohistochemistry-formalin-pfa-fixed-paraffin-embedded-sections-img118740.jpg "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SLP76 antibody [EPR2549(2)] - BSA and Azide free (ab247808)")
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SLP76 antibody [EPR2549(2)] - BSA and Azide free (ab247808)
This data was developed using Anti-SLP76 antibody [EPR2549(2)] ab109254, the same antibody clone in a different buffer formulation.Anti-SLP76 antibody [EPR2549(2)] ab109254 showing negative staining in Normal human heart tissue.
Perform heat mediated antigen retrieval before commencing with IHC staining protocol.
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SLP76 antibody [EPR2549(2)] - BSA and Azide free (ab247808), expandable thumbnail](https://content.abcam.com/products/images/slp76-antibody-epr25492-bsa-and-azide-free-ab247808--immunohistochemistry-formalin-pfa-fixed-paraffin-embedded-sections-img118740.jpg/_jcr_content/renditions/webp-475.webp "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SLP76 antibody [EPR2549(2)] - BSA and Azide free (ab247808)")
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