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AB206782

Anti-SLP76 (phospho Y145) antibody [EP2853Y] - BSA and Azide free

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(1 Publication)

Rabbit Recombinant Monoclonal SLP76 phospho Y145 antibody. Carrier free. Suitable for ICC/IF, Dot, WB, Flow Cyt (Intra) and reacts with Human, Synthetic peptide samples. Cited in 1 publication.

View Alternative Names

Lymphocyte cytosolic protein 2, SH2 domain-containing leukocyte protein of 76 kDa, SLP-76 tyrosine phosphoprotein, SLP76, LCP2

6 Images
Immunocytochemistry/ Immunofluorescence - Anti-SLP76 (phospho Y145) antibody [EP2853Y] - BSA and Azide free (AB206782)
  • ICC/IF

Unknown

Immunocytochemistry/ Immunofluorescence - Anti-SLP76 (phospho Y145) antibody [EP2853Y] - BSA and Azide free (AB206782)

This data was developed using ab75829, the same antibody clone in a different buffer formulation.

ab75829 staining SLP76 in Jurkat by Immunocytochemistry. Tissue was fixed with 4% paraformaldehyde. Samples were incubated with primary antibody (4.5 μg/ml). ab150077 Alexa Fluor® 488 Goat anti-Rabbit (1/1000) was used as the secondary antibody. ab195889 Anti-alpha Tubulin antibody [DM1A] - Microtubule Marker (Alexa Fluor® 594) at 2.5 μg/ml and DAPI were used as counter stains.

Confocal image showing the expression was increased after treatment with pervanadate (1mM, 30min) and then decreased after treatment with the Lambda Protein Phosphatase 31°C for 2h

Immunocytochemistry/ Immunofluorescence - Anti-SLP76 (phospho Y145) antibody [EP2853Y] - BSA and Azide free (AB206782)
  • ICC/IF

Unknown

Immunocytochemistry/ Immunofluorescence - Anti-SLP76 (phospho Y145) antibody [EP2853Y] - BSA and Azide free (AB206782)

This data was developed using ab75829, the same antibody clone in a different buffer formulation.

ab75829 staining SLP76 (phospho Y145) antibody in both treated (50mM of pervanadate for 5 minutes) and untreated Jurkat (human acute T cell leukemia) cells by ICC/IF (Immunocytochemistry/immunofluorescence). Cells were fixed with 4% Paraformaldehyde and permeabilized with 0.1% Triton X-100. Samples were incubated with primary antibody at a dilution of 1/500. A goat anti rabbit IgG (Alexa Fluor® 488) (ab150077) was used as the secondary antibody at a dilution of 1/1000.

DAPI was used as a nuclear counterstain.

Flow Cytometry (Intracellular) - Anti-SLP76 (phospho Y145) antibody [EP2853Y] - BSA and Azide free (AB206782)
  • Flow Cyt (Intra)

Unknown

Flow Cytometry (Intracellular) - Anti-SLP76 (phospho Y145) antibody [EP2853Y] - BSA and Azide free (AB206782)

This data was developed using ab75829, the same antibody clone in a different buffer formulation.

Intracellular flow cytometric analysis of permeabilized Jurkat cells, untreated (green) or pervanadate treated (red) using ab75829 at a 1/100 dilution. Also, pervanadate Jurkat cells using the same antibody preincubated with phospho SLP76 peptide (blue) or non phospho SLP76 peptide (orange).

Western blot - Anti-SLP76 (phospho Y145) antibody [EP2853Y] - BSA and Azide free (AB206782)
  • WB

Unknown

Western blot - Anti-SLP76 (phospho Y145) antibody [EP2853Y] - BSA and Azide free (AB206782)

This data was developed using ab75829, the same antibody clone in a different buffer formulation.

All lanes:

Western blot - Anti-SLP76 (phospho Y145) antibody [EP2853Y] (<a href='/en-us/products/primary-antibodies/slp76-phospho-y145-antibody-ep2853y-ab75829'>ab75829</a>) at 1/200000 dilution

Lane 1:

Jurkat cell lysate un-treated at 10 µg

Lane 2:

Jurkat cell lysate treated with pervanadate at 10 µg

Secondary

All lanes:

HRP labelled goat anti-rabbit at 1/2000 dilution

Predicted band size: 60 kDa

Observed band size: 76 kDa

false

Western blot - Anti-SLP76 (phospho Y145) antibody [EP2853Y] - BSA and Azide free (AB206782)
  • WB

Lab

Western blot - Anti-SLP76 (phospho Y145) antibody [EP2853Y] - BSA and Azide free (AB206782)

This data was developed using ab75829, the same antibody clone in a different buffer formulation.

Blocking and dilution buffer : 5% NFDM/TBST.

All lanes:

Western blot - Anti-SLP76 (phospho Y145) antibody [EP2853Y] (<a href='/en-us/products/primary-antibodies/slp76-phospho-y145-antibody-ep2853y-ab75829'>ab75829</a>) at 1/5000 dilution

Lane 1:

Jurkat (Human T cell leukemia T lymphocyte) whole cell lysates at 15 µg

Lane 2:

Jurkat (Human T cell leukemia T lymphocyte) whole cell lysates. treated with pervanadate at 50nM for 5 min at 15 µg

Lane 3:

Jurkat (Human T cell leukemia T lymphocyte) whole cell lysates. Treated with pervanadate at 50nM for 5 min. Then the membrane was incubated with phosphatase at 15 µg

Secondary

All lanes:

Western blot - Goat Anti-Rabbit IgG H&L (HRP) (<a href='/en-us/products/secondary-antibodies/goat-rabbit-igg-h-l-hrp-ab97051'>ab97051</a>) at 1/20000 dilution

Predicted band size: 60 kDa

Observed band size: 76 kDa

false

Dot Blot - Anti-SLP76 (phospho Y145) antibody [EP2853Y] - BSA and Azide free (AB206782)
  • Dot

Lab

Dot Blot - Anti-SLP76 (phospho Y145) antibody [EP2853Y] - BSA and Azide free (AB206782)

This data was developed using ab75829, the same antibody clone in a different buffer formulation.

Dot blot analysis of SLP76 (phospho Y145) phospho peptide (Lane 1), SLP76 Non-phospho peptide (Lane 2), labelling SLP76 (phospho Y145) with ab75829 at a dilution of 1/1000. Peroxidase conjugated goat anti-rabbit IgG (H+L)) was used as the secondary antibody at a dilution of 1/2500.

Blocking and diluting buffer : 5% NFDM/TBST.

Exposure time : 3 minutes.

Key facts

Host species

Rabbit

Clonality

Monoclonal

Clone number

EP2853Y

Isotype

IgG

Carrier free

Yes

Reacts with

Human

Applications

Dot, ICC/IF, Flow Cyt (Intra), WB

applications

Immunogen

The exact immunogen used to generate this antibody is proprietary information.

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Complementary Products

Secondary Antibodies

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Primary Antibodies

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  • 0
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Primary Antibodies

AB4448

Anti-Pericentrin antibody - Centrosome Marker

Lab Essentials

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  • 36
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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "ICCIF" : {"fullname" : "Immunocytochemistry/ Immunofluorescence", "shortname":"ICC/IF"}, "Dot" : {"fullname" : "Dot Blot", "shortname":"Dot"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"}, "FlowCytIntra" : {"fullname" : "Flow Cytometry (Intracellular)", "shortname":"Flow Cyt (Intra)"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "ICCIF-species-checked": "testedAndGuaranteed", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "<p></p>", "Dot-species-checked": "guaranteed", "Dot-species-dilution-info": "", "Dot-species-notes": "", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "", "WB-species-notes": "<p></p>", "IHCP-species-checked": "notRecommended", "IHCP-species-dilution-info": "", "IHCP-species-notes": "<p></p>", "FlowCytIntra-species-checked": "testedAndGuaranteed", "FlowCytIntra-species-dilution-info": "", "FlowCytIntra-species-notes": "<p></p>" }, "Synthetic peptide": { "ICCIF-species-checked": "notRecommended", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "", "Dot-species-checked": "testedAndGuaranteed", "Dot-species-dilution-info": "", "Dot-species-notes": "<p></p>", "WB-species-checked": "notRecommended", "WB-species-dilution-info": "", "WB-species-notes": "", "IHCP-species-checked": "notRecommended", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "FlowCytIntra-species-checked": "notRecommended", "FlowCytIntra-species-dilution-info": "", "FlowCytIntra-species-notes": "" } } }
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Product details

ab206782 is the carrier-free version of ab75829.

Species reactivity
Mouse, Rat: We have preliminary internal testing data to indicate this antibody may not react with these species.
Please contact us for more information.

Patented technology
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:

  • - High batch-to-batch consistency and reproducibility
  • - Improved sensitivity and specificity
  • - Long-term security of supply
  • - Animal-free batch production

For more information, read more on recombinant antibodies.

Conjugation ready
Our carrier-free antibodies are typically supplied in a PBS-only formulation, purified and free of BSA, sodium azide and glycerol. This conjugation-ready format is designed for use with fluorochromes, metal isotopes, oligonucleotides, and enzymes, which makes them ideal for antibody labelling, functional and cell-based assays, flow-based assays (e.g. mass cytometry) and Multiplex Imaging applications.

Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with 1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.

Compatibility
This product is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm, without the need for antibody preparation. Maxpar® is a trademark of Fluidigm Canada Inc.

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Properties and storage information

Form
Liquid
Purification technique
Affinity purification Protein A
Storage buffer
pH: 7.2 - 7.4 Constituents: PBS
Shipped at conditions
Blue Ice
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
+4°C
Storage information
Do Not Freeze
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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

SLP76 also known as Src homology 2 domain-containing leukocyte phosphoprotein of 76 kDa plays a pivotal role in intracellular signaling. This adaptor protein weighs approximately 76 kilodaltons and is predominantly expressed in hematopoietic cells particularly T cells NK cells mast cells and platelets. SLP76 functions to relay signals from the cell surface to the nucleus affecting cellular responses including activation proliferation and differentiation.
Biological function summary

This adaptor protein serves as a scaffold linking membrane-proximal signaling to downstream cellular effectors. SLP76 forms part of a larger signaling complex with proteins such as LAT (Linker for Activation of T cells) and Gads facilitating the assembly of multi-protein complexes necessary for signal transduction. It coordinates the recruitment and activation of several enzymes and substrates which leads to robust immune cell responses.

Pathways

The function of SLP76 integrates into T cell receptor (TCR) signaling and integrin signaling pathways. In TCR signaling SLP76 interacts closely with proteins LAT and ZAP-70 which are triggered upon antigen recognition. This interaction propagates the immune response by activating downstream effectors like MAP kinases and NF-kB. In integrin signaling SLP76 helps mediate cytoskeletal rearrangements which are important for cell migration and adhesion.

Mutations or dysregulation of SLP76 associate with immunodeficiencies and hematologic malignancies. Aberrant SLP76 activity can lead to inappropriate activation or inactivation of immune responses contributing to disorders as SCID (Severe Combined Immunodeficiency) and some types of leukemia. Additionally it connects with proteins such as LAT in disease contexts reflecting a shared pathway disruption contributing to the pathophysiology of these conditions.
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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:
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Target data

Adapter protein primarily involved in signaling pathways within T-cells, as well as other immune cells such as platelets, mast cells, and natural killer (NK) cells (PubMed : 11313406, PubMed : 33159816). Plays a crucial role for transducing signal from the T-cell receptor (TCR) after antigen recognition leading to T-cell activation. Mechanistically, once phosphorylated by the kinase ZAP70, mediates interactions with the guanine-nucleotide exchange factor VAV1, the adapter protein NCK and the kinase ITK (PubMed : 8673706, PubMed : 8702662). In turn, stimulates the activation of PKC-theta/PRKCQ and NF-kappa-B transcriptional activity in response to CD3 and CD28 costimulation (PubMed : 11313406). Also plays an essential role in AGER-induced signaling pathways including p38 MAPK and ERK1/2 activation leading to cytokine release and pro-inflammatory responses (PubMed : 33436632).
See full target information LCP2 pY145
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Publications (1)

Recent publications for all applications. Explore the full list and refine your search

Nature methods 21:1708-1715 PubMed39117875

2024

ProDOL: a general method to determine the degree of labeling for staining optimization and molecular counting.

Applications

Unspecified application

Species

Unspecified reactive species

Stanimir Asenov Tashev,Jonas Euchner,Klaus Yserentant,Siegfried Hänselmann,Felix Hild,Wioleta Chmielewicz,Johan Hummert,Florian Schwörer,Nikolaos Tsopoulidis,Stefan Germer,Zoe Saßmannshausen,Oliver T Fackler,Ursula Klingmüller,Dirk-Peter Herten
View all publications
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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.
For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

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