Rabbit Polyclonal SLPI antibody. Suitable for WB and reacts with Human samples. Cited in 2 publications. Immunogen corresponding to Synthetic Peptide within Human SLPI.
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Publications
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- PloS one 9:e953892014Quantitative proteomic analysis of oral brush biopsies identifies secretory leukocyte protease inhibitor as a promising, mechanism-based oral cancer biomarker.
- Blood 123:1239-492013A lack of secretory leukocyte protease inhibitor (SLPI) causes defects in granulocytic differentiation.Applications:Unspecified applicationReactive species:HumanOlga Klimenkova,Wienke Ellerbeck,Maksim Klimiankou,Murat Ünalan,Siarhei Kandabarau,Anna Gigina,Kais Hussein,Cornelia Zeidler,Karl Welte,Julia SkokowaPubMed 24352879
Key facts
- Isotype
- IgG
- Host species
- Rabbit
- Storage buffer
pH: 7.4
Preservative: 0.05% Sodium azide
Constituents: PBS, 50% Glycerol (glycerin, glycerine), 2.9% Sodium chloride- Form
- Liquid
- Clonality
- Polyclonal
Immunogen
- Synthetic Peptide within Human SLPI. The exact immunogen used to generate this antibody is proprietary information. Database link P03973
Reactivity data
Select an application| WB | |
|---|---|
| Human | Tested |
WB
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info 1/1000.00000 - 1/5000.00000 | Notes A recommended starting concentration for Western blots is 1/1000 when using colorimetric substrates such as BCIP/NBT, and 1/5000 for chemiluminescent substrates. Dilution optimised using Chromogenic detection. |
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Target data
Function
Acid-stable proteinase inhibitor with strong affinities for trypsin, chymotrypsin, elastase, and cathepsin G (PubMed:10702419, PubMed:2039600, PubMed:2110563, PubMed:24121345, PubMed:3462719, PubMed:3533531). Modulates the inflammatory and immune responses after bacterial infection, and after infection by the intracellular parasite L.major. Down-regulates responses to bacterial lipopolysaccharide (LPS) (By similarity). Plays a role in regulating the activation of NF-kappa-B and inflammatory responses (PubMed:10702419, PubMed:24352879). Has antimicrobial activity against mycobacteria, but not against salmonella. Contributes to normal resistance against infection by M.tuberculosis. Required for normal resistance to infection by L.major. Required for normal wound healing, probably by preventing tissue damage by limiting protease activity (By similarity). Together with ELANE, required for normal differentiation and proliferation of bone marrow myeloid cells (PubMed:24352879).
Alternative names
WAP4, WFDC4, SLPI, Antileukoproteinase, ALP, BLPI, HUSI-1, Mucus proteinase inhibitor, Protease inhibitor WAP4, Secretory leukocyte protease inhibitor, Seminal proteinase inhibitor, WAP four-disulfide core domain protein 4, MPI
Recommended products
Rabbit Polyclonal SLPI antibody. Suitable for WB and reacts with Human samples. Cited in 2 publications. Immunogen corresponding to Synthetic Peptide within Human SLPI.
Key facts
- Isotype
- IgG
- Host species
- Rabbit
- Storage buffer
pH: 7.4
Preservative: 0.05% Sodium azide
Constituents: PBS, 50% Glycerol (glycerin, glycerine), 2.9% Sodium chloride- Form
- Liquid
- Clonality
- Polyclonal
- Immunogen
- Synthetic Peptide within Human SLPI. The exact immunogen used to generate this antibody is proprietary information. Database link P03973
- Purification technique
- Affinity purification Immunogen
- Concentration
Storage
- Shipped at conditions
- Blue Ice
- Appropriate short-term storage conditions
- +4°C
- Appropriate long-term storage conditions
- -20°C
- Aliquoting information
- Upon delivery aliquot
- Storage information
- Avoid freeze / thaw cycle
Notes
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Supplementary info
- This supplementary information is collated from multiple sources and compiled automatically.
- Activity summary
Secretory leukocyte protease inhibitor (SLPI) also known as antileukoproteinase and neutrophil elastase inhibitor is a protein with a molecular mass of approximately 12 kDa. SLPI is found mainly in epithelial tissues and is expressed in locations such as the respiratory tract skin and cervix. Mechanically SLPI functions as a serine protease inhibitor binding to and neutralizing enzymes like neutrophil elastase cathepsin G and trypsin which helps to limit tissue damage during inflammation.
- Biological function summary
SLPI serves as a protector of epithelial surfaces from proteolytic degradation and acts as an anti-inflammatory agent. It displays broad-spectrum antimicrobial properties providing protection against bacterial and viral infections. Although SLPI is not part of a complex itself its interactions with extracellular matrix proteins help modulate wound healing processes and maintain tissue integrity.
- Pathways
SLPI participates in the innate immune response and inflammation regulation pathways. It interacts within pathways that involve the complement cascade and kallikrein-kinin system helping to regulate inflammation and modulate immune responses. During these processes SLPI is related to proteins such as alpha-1-antitrypsin in its role as a protease inhibitor contributing to the regulation of protease activity and inflammatory responses.
- Associated diseases and disorders
SLPI is implicated in conditions such as chronic obstructive pulmonary disease (COPD) and cystic fibrosis where excessive protease activity leads to tissue damage. In these diseases SLPI shows a protective role against lung tissue degradation. SLPI is also associated with the progression of certain cancers like ovarian cancer where its overexpression may support tumor growth and metastasis. In these contexts SLPI relates to other protease inhibitors such as tissue inhibitors of metalloproteinases (TIMPs) due to their shared function in regulating protease activity and tissue remodeling.
Product promise
Tested
We have tested this species and application combination and it works. It is covered by our product promise.
Expected
We have not tested this specific species and application combination in-house, but expect it will work. It is covered by our product promise.
Predicted
This species and application combination has not been tested, but we predict it will work based on strong homology. However, this combination is not covered by our product promise.
Not recommended
We do not recommend this combination. It is not covered by our product promise.
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2 product images
Western blot - Anti-SLPI antibody - Aminoterminal end (ab46763)
False colour image of Western blot: Anti-SLPI antibody - Aminoterminal end staining at 1/1000 dilution, shown in green; Mouse anti-GAPDH antibody [6C5] (Anti-GAPDH antibody [6C5] - Loading Control ab8245) loading control staining at 1/20000 dilution, shown in red. In Western blot, ab46763 was shown to bind specifically to SLPI. A band was observed at 22 kDa in wild-type HeLa cell lysates with no signal observed at this size in SLPI knockout cell line Human SLPI knockout HeLa cell line ab265215 (knockout cell lysate Human SLPI knockout HeLa cell lysate ab257685). To generate this image, wild-type and SLPI knockout HeLa cell lysates were analysed. First, samples were run on an SDS-PAGE gel then transferred onto a nitrocellulose membrane. Membranes were blocked in fluorescent western blot (TBS-based) blocking solution before incubation with primary antibodies overnight at 4 °C. Blots were washed four times in TBS-T, incubated with secondary antibodies for 1 h at room temperature, washed again four times then imaged.Secondary antibodies used were Goat anti-Rabbit IgG H&L (IRDye®800CW) preabsorbed (Goat anti-Rabbit IgG H&L (IRDye® 800CW) preadsorbed ab216773) and Goat anti-Mouse IgG H&L (IRDye® 680RD) preabsorbed (Goat anti-Mouse IgG H&L (IRDye® 680RD) preadsorbed ab216776) at 1/20000 dilution.
All lanes: Western blot - Anti-SLPI antibody - Aminoterminal end (ab46763) at 1/1000 dilution
Lane 1: Wild-type HeLa cell lysate at 20 µg
Lane 2: SLPI knockout HeLa cell lysate at 20 µg
Lane 2: Western blot - Human SLPI knockout HeLa cell line (Human SLPI knockout HeLa cell line ab265215)
Lane 2: Western blot - Human SLPI knockout HeLa cell lysate (Human SLPI knockout HeLa cell lysate ab257685)
Lane 3: PC-3 cell lysate at 20 µg
Lane 4: LNCaP cell lysate at 20 µg
Performed under reducing conditions.
Predicted band size: 14 kDa
Observed band size: 22 kDa
Western blot - Anti-SLPI antibody - Aminoterminal end (ab46763)
All lanes: Western blot - Anti-SLPI antibody - Aminoterminal end (ab46763) at 1/1000 dilution
Lane 1: Recombinant Human SLP I at 0.04 µg
Lane 2: Recombinant Human SLP I at 0.02 µg
Lane 3: Recombinant Human SLP I at 0.01 µg
Lane 4: Recombinant Human SLP I at 0.004 µg
Lane 5: Recombinant Human SLP I at 0.001 µg
Predicted band size: 14 kDa
Observed band size: 15 kDa
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