Anti-SLPI antibody - Aminoterminal end

• WB

Lab

Western blot - Anti-SLPI antibody - Aminoterminal end (AB46763)

False colour image of Western blot : Anti-SLPI antibody - Aminoterminal end staining at 1/1000 dilution, shown in green; Mouse anti-GAPDH antibody [6C5] (ab8245) loading control staining at 1/20000 dilution, shown in red. In Western blot, ab46763 was shown to bind specifically to SLPI. A band was observed at 22 kDa in wild-type HeLa cell lysates with no signal observed at this size in SLPI knockout cell line ab265215 (knockout cell lysate ab257685). To generate this image, wild-type and SLPI knockout HeLa cell lysates were analysed. First, samples were run on an SDS-PAGE gel then transferred onto a nitrocellulose membrane. Membranes were blocked in fluorescent western blot (TBS-based) blocking solution before incubation with primary antibodies overnight at 4 °C. Blots were washed four times in TBS-T, incubated with secondary antibodies for 1 h at room temperature, washed again four times then imaged.Secondary antibodies used were Goat anti-Rabbit IgG H&L (IRDye^®800CW) preabsorbed (ab216773) and Goat anti-Mouse IgG H&L (IRDye^® 680RD) preabsorbed (ab216776) at 1/20000 dilution.

All lanes:

Western blot - Anti-SLPI antibody - Aminoterminal end (ab46763) at 1/1000 dilution

Lane 1:

Wild-type HeLa cell lysate at 20 µg

Lane 2:

SLPI knockout HeLa cell lysate at 20 µg

Lane 2:

Western blot - Human SLPI knockout HeLa cell line (<a href='/en-us/products/cell-lines/human-slpi-knockout-hela-cell-line-ab265215'>ab265215</a>)

Lane 2:

Western blot - Human SLPI knockout HeLa cell lysate (<a href='/en-us/products/cell-lysates/human-slpi-knockout-hela-cell-lysate-ab257685'>ab257685</a>)

Lane 3:

PC-3 cell lysate at 20 µg

Lane 4:

LNCaP cell lysate at 20 µg

Predicted band size: 14 kDa

Observed band size: 22 kDa

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• WB

Unknown

Western blot - Anti-SLPI antibody - Aminoterminal end (AB46763)

All lanes:

Western blot - Anti-SLPI antibody - Aminoterminal end (ab46763) at 1/1000 dilution

Lane 1:

Recombinant Human SLP I at 0.04 µg

Lane 2:

Recombinant Human SLP I at 0.02 µg

Lane 3:

Recombinant Human SLP I at 0.01 µg

Lane 4:

Recombinant Human SLP I at 0.004 µg

Lane 5:

Recombinant Human SLP I at 0.001 µg

Predicted band size: 14 kDa

Observed band size: 15 kDa

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