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AB250374

Anti-SLU7 antibody [EPR11111] - BSA and Azide free

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Rabbit Recombinant Monoclonal SLU7 antibody. Carrier free. Suitable for ICC/IF, WB and reacts with Human samples.

View Alternative Names

Pre-mRNA-splicing factor SLU7, hSlu7, SLU7

3 Images
Immunocytochemistry/ Immunofluorescence - Anti-SLU7 antibody [EPR11111] - BSA and Azide free (AB250374)
  • ICC/IF

Supplier Data

Immunocytochemistry/ Immunofluorescence - Anti-SLU7 antibody [EPR11111] - BSA and Azide free (AB250374)

This data was developed using ab181138, the same antibody clone in a different buffer formulation.

Immunofluorescent analysis of 293 cells (paraformaldehyde-fixed, 4%) labeling SLU7 with ab181138 at 1/250 dilution followed by Goat anti rabbit IgG (Alexa Fluor® 555) secondary at 1/200 dilution and counter-stained with DAPI (blue).

Western blot - Anti-SLU7 antibody [EPR11111] - BSA and Azide free (AB250374)
  • WB

Supplier Data

Western blot - Anti-SLU7 antibody [EPR11111] - BSA and Azide free (AB250374)

This data was developed using ab181138, the same antibody clone in a different buffer formulation.

All lanes:

Western blot - Anti-SLU7 antibody [EPR11111] (<a href='/en-us/products/primary-antibodies/slu7-antibody-epr11111-ab181138'>ab181138</a>) at 1/2000 dilution

Lane 1:

HeLa cell lysate at 20 µg

Lane 2:

293 cell lysate at 20 µg

Secondary

All lanes:

Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugate at 1/1000 dilution

Predicted band size: 68 kDa

false

Western blot - Anti-SLU7 antibody [EPR11111] - BSA and Azide free (AB250374)
  • WB

Supplier Data

Western blot - Anti-SLU7 antibody [EPR11111] - BSA and Azide free (AB250374)

This data was developed using ab181138, the same antibody clone in a different buffer formulation.

All lanes:

Western blot - Anti-SLU7 antibody [EPR11111] (<a href='/en-us/products/primary-antibodies/slu7-antibody-epr11111-ab181138'>ab181138</a>) at 1/10000 dilution

All lanes:

Jurkat cell lysate at 20 µg

Secondary

All lanes:

Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugate at 1/1000 dilution

Predicted band size: 68 kDa

false

Key facts

Host species

Rabbit

Clonality

Monoclonal

Clone number

EPR11111

Isotype

IgG

Carrier free

Yes

Reacts with

Human

Applications

ICC/IF, WB

applications

Immunogen

The exact immunogen used to generate this antibody is proprietary information.

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "ICCIF" : {"fullname" : "Immunocytochemistry/ Immunofluorescence", "shortname":"ICC/IF"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "ICCIF-species-checked": "testedAndGuaranteed", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "<p></p>", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "", "WB-species-notes": "<p></p>" }, "Rat": { "ICCIF-species-checked": "predicted", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "" } } }
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Product details

ab250374 is the carrier-free version of ab181138.

Patented technology
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:

  • - High batch-to-batch consistency and reproducibility
  • - Improved sensitivity and specificity
  • - Long-term security of supply
  • - Animal-free batch production

For more information, read more on recombinant antibodies.

Conjugation ready
Our carrier-free antibodies are typically supplied in a PBS-only formulation, purified and free of BSA, sodium azide and glycerol. This conjugation-ready format is designed for use with fluorochromes, metal isotopes, oligonucleotides, and enzymes, which makes them ideal for antibody labelling, functional and cell-based assays, flow-based assays (e.g. mass cytometry) and Multiplex Imaging applications.

Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with 1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.

Compatibility
This product is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm, without the need for antibody preparation. Maxpar® is a trademark of Fluidigm Canada Inc.

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Properties and storage information

Form
Liquid
Purification technique
Affinity purification
Storage buffer
pH: 7.2 - 7.4 Constituents: PBS
Shipped at conditions
Blue Ice
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
+4°C
Storage information
Do Not Freeze
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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

SLU7 also known as Splicing Factor SLU7 is an essential component of the pre-mRNA splicing process. Weighing approximately 65 kDa SLU7 is mainly found in the nucleus of cells where splicing occurs. It plays an important role in the second catalytic step of alternative splicing by ensuring accurate 3' splice site selection. SLU7 assists in proper intron removal and exon joining during mRNA processing ensuring the production of mature mRNA. Expression of SLU7 is observed in various tissues but high levels are noted in the brain and liver.
Biological function summary

SLU7 influences the regulation of gene expression by participating in mRNA splicing. As part of the larger spliceosome complex SLU7 helps with the recognition of splice sites addressing both simple and more complex pre-mRNA structures. Its function impacts various cellular processes by modulating the diversity of protein products through alternative splicing. This diversity is important for cells to adapt to developmental cues and environmental changes impacting cell differentiation and the response to stress.

Pathways

SLU7 functions significantly in mRNA processing and RNA transport pathways. It interacts with other splicing factors such as U2AF35 and U2AF65 forming a network important for the precise removal of introns from pre-mRNA. SLU7's involvement in these pathways affects numerous cellular transcription-related processes and connects with gene regulatory networks influencing the broader system of gene expression.

Errors in SLU7 function have links to neurodegenerative diseases and certain cancers. In neurodegenerative conditions such as Alzheimer's disease altered splicing patterns due to SLU7 malfunction may contribute to the pathology. In cancer aberrant expression or mutations in SLU7 could disrupt normal splicing affecting cellular growth and tumor progression. Connections are seen with RBM17 and other spliceosomal proteins where deregulation can exacerbate disease development and progression.
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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:
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Target data

Required for pre-mRNA splicing as component of the spliceosome (PubMed : 10197984, PubMed : 28502770, PubMed : 30705154). Participates in the second catalytic step of pre-mRNA splicing, when the free hydroxyl group of exon I attacks the 3'-splice site to generate spliced mRNA and the excised lariat intron. Required for holding exon 1 properly in the spliceosome and for correct AG identification when more than one possible AG exists in 3'-splicing site region. May be involved in the activation of proximal AG. Probably also involved in alternative splicing regulation.
See full target information Pre-mRNA-splicing factor SLU7
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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.
For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

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