Anti-SLUG antibody

• WB

AbReview43125****

Western blot - Anti-SLUG antibody (AB106077)

Blocked with 5% milk for 1 hour at 22°C.

Primary incubation at 4°C for 18 hours.

All lanes:

Western blot - Anti-SLUG antibody (ab106077) at 1/2000 dilution

Lane 1:

MDA-MB-231 whole cell lysate at 30 µg

Lane 2:

MDA-MB-231 whole cell lysate - sh Slug 1 at 30 µg

Lane 3:

MDA-MB-231 whole cell lysate - sh Slug 2 at 30 µg

Lane 4:

MDA-MB-231 whole cell lysate - sh Snail 1 at 30 µg

Secondary

All lanes:

HRP-conjugated goat anti-rabbit IgG polyclonal at 1/5000 dilution

Predicted band size: 29 kDa

Observed band size: 35 kDa,50 kDa,65 kDa

true

Exposure time: 30s

This image is courtesy of an anonymous Abreview

• WB

Unknown

Western blot - Anti-SLUG antibody (AB106077)

All lanes:

Western blot - Anti-SLUG antibody (ab106077) at 1 µg/mL

Lane 1:

MCF7 cell lysate overexpressing SLUG protein at 10 µg

Lane 2:

MCF7 cell lysate overexpressing SNAIL protein at 10 µg

Lane 3:

MCF7 cell lysate transfected with vector control plasmid at 10 µg

Lane 4:

Untransfected MCF7 cell lysate at 10 µg

Secondary

All lanes:

Western blot - Goat Anti-Rabbit IgG H&L (HRP) preadsorbed (<a href='/en-us/products/secondary-antibodies/goat-rabbit-igg-h-l-hrp-preadsorbed-ab97080'>ab97080</a>) at 1/5000 dilution

Predicted band size: 29 kDa

Observed band size: 36 kDa

true

Exposure time: 8min