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AB183760

Anti-SLUG antibody

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(9 Publications)

Rabbit Polyclonal SLUG antibody. Suitable for WB and reacts with Human samples. Cited in 9 publications. Immunogen corresponding to Recombinant Fragment Protein within Human SNAI2 aa 100 to C-terminus.

View Alternative Names

SLUG, SLUGH, SNAI2, Zinc finger protein SNAI2, Neural crest transcription factor Slug, Protein snail homolog 2

1 Images
Western blot - Anti-SLUG antibody (AB183760)
  • WB

Supplier Data

Western blot - Anti-SLUG antibody (AB183760)

All lanes:

Western blot - Anti-SLUG antibody (ab183760) at 1/5000 dilution

Lane 1:

293T whole cell lysate/extract at 30 µg

Lane 2:

Whole cell lysate/extract of Human SLUG-transfected 293T cells at 30 µg

Predicted band size: 29 kDa

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Key facts

Host species

Rabbit

Clonality

Polyclonal

Isotype

IgG

Carrier free

No

Reacts with

Human

Applications

WB

applications

Immunogen

Recombinant Fragment Protein within Human SNAI2 aa 100 to C-terminus. The exact immunogen used to generate this antibody is proprietary information.

O43623

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Reactivity data

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Properties and storage information

Form
Liquid
Purification technique
Affinity purification Immunogen
Storage buffer
pH: 7 Preservative: 0.01% Thimerosal (merthiolate) Constituents: PBS, 20% Glycerol (glycerin, glycerine)
Shipped at conditions
Blue Ice
Appropriate short-term storage duration
1-2 weeks
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
-20°C
Aliquoting information
Upon delivery aliquot
Storage information
Avoid freeze / thaw cycle
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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

SLUG also known as SNAI2 is a zinc finger transcription factor that plays a mechanical role in the regulation of genes implicated in cell differentiation and development. With an approximate mass of 29 kDa SLUG is expressed in various tissues particularly in the neural crest and epithelial-cell precursors. It acts as a repressor by binding to E-box motifs in the promoter regions of its target genes. SLUG functions in coordination with other cofactors to modulate gene expression that influences diverse biological processes including epithelial-mesenchymal transition (EMT).
Biological function summary

SLUG influences cell motility and invasion by controlling EMT a process critical for development and cancer metastasis. SLUG functions as a part of the Snail family of transcription factors and collaborates with other EMT-related molecules. It influences the expression of genes that maintain the epithelial phenotype facilitating the switch to a mesenchymal state required for increased cell mobility. Through these actions SLUG participates in the dynamic remodeling of tissues and is an important player during embryonic development and in certain pathological conditions.

Pathways

Researchers have associated SLUG with the TGF-beta and Wnt signaling pathways both essential for cell growth and differentiation. These pathways support SLUG's role in promoting EMT by modulating its transcriptional activity. SLUG often interacts with proteins such as TWIST1 and ZEB1 which synergistically act to downregulate epithelial markers and upregulate mesenchymal markers. This synergy highlights SLUG's significant role in facilitating changes in cellular architecture and function.

SLUG shows a strong connection with the progression of cancers and fibrosis. It contributes to cancer metastasis through its competence in inducing EMT enabling tumor cells to invade and establish secondary tumors. In breast cancer SLUG correlates with increased invasiveness and poor prognosis. In fibrosis SLUG promotes tissue scarring by facilitating fibroblast activation and extracellular matrix deposition. SLUG has interactions with other proteins such as E-Cadherin where its repressing activity on E-Cadherin contributes to the disruption of cell-cell adhesion a hallmark of metastatic cells.
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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:
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Target data

Transcriptional repressor that modulates both activator-dependent and basal transcription. Involved in the generation and migration of neural crest cells. Plays a role in mediating RAF1-induced transcriptional repression of the TJ protein, occludin (OCLN) and subsequent oncogenic transformation of epithelial cells (By similarity). Represses BRCA2 expression by binding to its E2-box-containing silencer and recruiting CTBP1 and HDAC1 in breast cells. In epidermal keratinocytes, binds to the E-box in ITGA3 promoter and represses its transcription. Involved in the regulation of ITGB1 and ITGB4 expression and cell adhesion and proliferation in epidermal keratinocytes. Binds to E-box2 domain of BSG and activates its expression during TGFB1-induced epithelial-mesenchymal transition (EMT) in hepatocytes. Represses E-Cadherin/CDH1 transcription via E-box elements. Involved in osteoblast maturation. Binds to RUNX2 and SOC9 promoters and may act as a positive and negative transcription regulator, respectively, in osteoblasts. Binds to CXCL12 promoter via E-box regions in mesenchymal stem cells and osteoblasts. Plays an essential role in TWIST1-induced EMT and its ability to promote invasion and metastasis.
See full target information SNAI2
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Publications (9)

Recent publications for all applications. Explore the full list and refine your search

Molecular cancer research : MCR 21:1107-1119 PubMed37409971

2023

TRIM50 Inhibits Gastric Cancer Progression by Regulating the Ubiquitination and Nuclear Translocation of JUP.

Applications

Unspecified application

Species

Unspecified reactive species

Jiajia Hu,Runjie Huang,Chengcai Liang,Yingnan Wang,Min Wang,Yanxing Chen,Chenyi Wu,Jinling Zhang,Zekun Liu,Qi Zhao,Zexian Liu,Feng Wang,Shuqiang Yuan

BioFactors (Oxford, England) 48:164-180 PubMed34882869

2021

LncRNA LOC100507144 acts as a novel regulator of CD44/Nanog/Sox2/miR-302/miR-21 axis in colorectal cancer.

Applications

Unspecified application

Species

Unspecified reactive species

Nasim Ebrahimi,Hajar Rezanejad,Malek Hossein Asadi,Sadeq Vallian

Discover. Oncology 12:12 PubMed35201457

2021

HOXC10 promotes growth and migration of melanoma by regulating Slug to activate the YAP/TAZ signaling pathway.

Applications

Unspecified application

Species

Unspecified reactive species

Yuanxin Miao,Weina Zhang,Su Liu,Xiangfeng Leng,Chunnan Hu,Hao Sun

Aging 11:7914-7937 PubMed31562290

2019

TIP-B1 promotes kidney clear cell carcinoma growth and metastasis via EGFR/AKT signaling.

Applications

Unspecified application

Species

Unspecified reactive species

Lei Yin,Shenglin Gao,Heng Shi,Keyi Wang,Huan Yang,Bo Peng

Journal of cellular biochemistry 120:17709-17722 PubMed31161607

2019

lncRNA MEG3 modified epithelial-mesenchymal transition of ovarian cancer cells by sponging miR-219a-5p and regulating EGFR.

Applications

Unspecified application

Species

Unspecified reactive species

Lei Wang,Mingxin Yu,Shanshan Zhao

Life sciences 226:149-155 PubMed30981764

2019

Knockdown of Delta-like 3 restricts lipopolysaccharide-induced inflammation, migration and invasion of A2058 melanoma cells via blocking Twist1-mediated epithelial-mesenchymal transition.

Applications

Unspecified application

Species

Unspecified reactive species

Xiaojie Ding,Fuyao Li,Li Zhang

International journal of oncology 53:2671-2682 PubMed30272271

2018

Long non-coding RNA CUDR promotes malignant phenotypes in pancreatic ductal adenocarcinoma via activating AKT and ERK signaling pathways.

Applications

Unspecified application

Species

Unspecified reactive species

Xing Liang,Meiyan Qi,Rui Wu,Anan Liu,Danlei Chen,Liang Tang,Jun Chen,Xiangui Hu,Wei Li,Lixing Zhan,Chenghao Shao

Journal of cellular biochemistry 119:6935-6942 PubMed29693289

2018

LncRNA ATB promotes proliferation and metastasis in A549 cells by down-regulation of microRNA-494.

Applications

Unspecified application

Species

Unspecified reactive species

Yiwei Cao,Xiangjun Luo,Xiaoqian Ding,Shichao Cui,Caihong Guo

Cancer medicine 7:2485-2503 PubMed29663730

2018

MiR-21 improves invasion and migration of drug-resistant lung adenocarcinoma cancer cell and transformation of EMT through targeting HBP1.

Applications

WB

Species

Human

Chongyu Su,Xu Cheng,Yunsong Li,Yi Han,Xiaoyun Song,Daping Yu,Xiaoqing Cao,Zhidong Liu
View all publications
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