JavaScript is disabled in your browser. Please enable JavaScript to view this website.
AB27568

Anti-SLUG antibody

4

(11 Reviews)

|

(206 Publications)

Rabbit Polyclonal SLUG antibody. Suitable for WB, IHC-Fr and reacts with Mouse, Rat, Human, Chicken samples. Cited in 206 publications.

View Alternative Names

SLUG, SLUGH, SNAI2, Zinc finger protein SNAI2, Neural crest transcription factor Slug, Protein snail homolog 2

4 Images
Immunocytochemistry/ Immunofluorescence - Anti-SLUG antibody (AB27568)
  • ICC/IF

AbReview10629****

Immunocytochemistry/ Immunofluorescence - Anti-SLUG antibody (AB27568)

ab27568 staining mouse embryonic stem cells by ICC/IF. Cells were formaldehyde fixed and permeabilized in 0.1% Triton X-100 prior to blocking with 0.1% BSA and 1% serum for 30 minutes at 21°C. The primary antibody was diluted 1/100 and incubated for 2 hours at 21°C. An Alexa Fluor® 488 conjugated goat anti-rabbit antibody, diluted 1/500, was used as the secondary.

This image is courtesy of an anonymous Abreview

Western blot - Anti-SLUG antibody (AB27568)
  • WB

Collaborator

Western blot - Anti-SLUG antibody (AB27568)

ab27568 detects a band of 34 kDa in MCF7 cells that have been transfected with a vector that overexpresses the SLUG protein. This antibody does not crossreact with the SNAIL protein, as it did not recognize this protein in MCF7 cells overexpressing SNAIL. ab27568 was unable to detect endogenous SLUG in MDA-MB231 cells (not shown).

All lanes:

Western blot - Anti-SLUG antibody (ab27568) at 1/1000 dilution

Lane 1:

MCF7 cell lysate overexpressing SLUG protein

Lane 2:

MCF7 lysate overexpressing SNAIL protein

Lane 3:

MCF7 lysate tranfected with vector control plasmid

Secondary

All lanes:

HRP-conjugated anti-rabbit antibody at 1/10000 dilution

Predicted band size: 29 kDa

Observed band size: 34 kDa

false

This image is courtesy of Archana Dhasarathy, Ph.D., NC

Western blot - Anti-SLUG antibody (AB27568)
  • WB

Unknown

Western blot - Anti-SLUG antibody (AB27568)

Secondary antibody - goat anti-rabbit HRP (preadsorbed) (ab97080)

All lanes:

Western blot - Anti-SLUG antibody (ab27568) at 1 µg/mL

Lane 1:

Heart (Mouse) Tissue Lysate at 30 µg

Lane 2:

Heart (Rat) Tissue Lysate at 30 µg

Secondary

All lanes:

Western blot - Goat Anti-Rabbit IgG H&L (HRP) preadsorbed (<a href='/en-us/products/secondary-antibodies/goat-rabbit-igg-h-l-hrp-preadsorbed-ab97080'>ab97080</a>) at 1/5000 dilution

Predicted band size: 29 kDa

Observed band size: 30 kDa

false

Immunohistochemistry (Frozen sections) - Anti-SLUG antibody (AB27568)
  • IHC-Fr

Collaborator

Immunohistochemistry (Frozen sections) - Anti-SLUG antibody (AB27568)

Transverse section showing Slug-expressing neural crest cells (green) in the dorsal neural tube (NT) in the hindbrain of a quail embryo. 4% PFA fixed embryonic day 1.7 Japanese Quail embryo, frozen in Tissutek, sectioned 12 um. ab27568 1/400, incubated overnight. Goat anti-rabbit : Alexa488 (Molecular Probes) 1/1000 incubated for 2 hours.

This image is courtesy of Don Newgreen and Dong Cheng Zhang, Murdoch Childrens Research Institute

Key facts

Host species

Rabbit

Clonality

Polyclonal

Isotype

IgG

Carrier free

No

Reacts with

Mouse, Rat, Human, Chicken

Applications

WB, IHC-Fr

applications

Immunogen

The exact immunogen used to generate this antibody is proprietary information.

Specificity

ab27568 detects the overexpressing human Slug protein, however we have no data on the detection of the endogenous Human protein with this current batch. ab27568 does not cross react with overexpressed SNAIL protein in cell lysates.

style
left-column

Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "ICC" : {"fullname" : "Immunocytochemistry", "shortname":"ICC"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "IHCFr" : {"fullname" : "Immunohistochemistry (Frozen sections)", "shortname":"IHC-Fr"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "ICC-species-checked": "notRecommended", "ICC-species-dilution-info": "", "ICC-species-notes": "<p></p>", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/500 - 1/1000", "WB-species-notes": "<p></p>", "IHCFr-species-checked": "guaranteed", "IHCFr-species-dilution-info": "", "IHCFr-species-notes": "<p></p>" }, "Mouse": { "ICC-species-checked": "notRecommended", "ICC-species-dilution-info": "", "ICC-species-notes": "<p></p>", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/500 - 1/1000", "WB-species-notes": "<p></p>", "IHCFr-species-checked": "guaranteed", "IHCFr-species-dilution-info": "", "IHCFr-species-notes": "<p></p>" }, "Rat": { "ICC-species-checked": "notRecommended", "ICC-species-dilution-info": "", "ICC-species-notes": "<p></p>", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/500 - 1/1000", "WB-species-notes": "<p></p>", "IHCFr-species-checked": "guaranteed", "IHCFr-species-dilution-info": "", "IHCFr-species-notes": "<p></p>" }, "Chicken": { "ICC-species-checked": "notRecommended", "ICC-species-dilution-info": "", "ICC-species-notes": "<p></p>", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "", "IHCFr-species-checked": "guaranteed", "IHCFr-species-dilution-info": "", "IHCFr-species-notes": "<p></p>" }, "Cow": { "ICC-species-checked": "notRecommended", "ICC-species-dilution-info": "", "ICC-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "", "IHCFr-species-checked": "predicted", "IHCFr-species-dilution-info": "", "IHCFr-species-notes": "" }, "Dog": { "ICC-species-checked": "notRecommended", "ICC-species-dilution-info": "", "ICC-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "", "IHCFr-species-checked": "predicted", "IHCFr-species-dilution-info": "", "IHCFr-species-notes": "" }, "Pig": { "ICC-species-checked": "notRecommended", "ICC-species-dilution-info": "", "ICC-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "", "IHCFr-species-checked": "predicted", "IHCFr-species-dilution-info": "", "IHCFr-species-notes": "" } } }
style
left-column
style
left-column

Properties and storage information

Form
Liquid
Purification technique
Affinity purification Immunogen
Storage buffer
pH: 7.4 Preservative: 0.02% Sodium azide Constituents: PBS, 1% BSA
Shipped at conditions
Blue Ice
Appropriate short-term storage duration
1-2 weeks
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
-20°C
Aliquoting information
Upon delivery aliquot
Storage information
Avoid freeze / thaw cycle
style
left-column

Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

SLUG also known as SNAI2 is a zinc finger transcription factor that plays a mechanical role in the regulation of genes implicated in cell differentiation and development. With an approximate mass of 29 kDa SLUG is expressed in various tissues particularly in the neural crest and epithelial-cell precursors. It acts as a repressor by binding to E-box motifs in the promoter regions of its target genes. SLUG functions in coordination with other cofactors to modulate gene expression that influences diverse biological processes including epithelial-mesenchymal transition (EMT).
Biological function summary

SLUG influences cell motility and invasion by controlling EMT a process critical for development and cancer metastasis. SLUG functions as a part of the Snail family of transcription factors and collaborates with other EMT-related molecules. It influences the expression of genes that maintain the epithelial phenotype facilitating the switch to a mesenchymal state required for increased cell mobility. Through these actions SLUG participates in the dynamic remodeling of tissues and is an important player during embryonic development and in certain pathological conditions.

Pathways

Researchers have associated SLUG with the TGF-beta and Wnt signaling pathways both essential for cell growth and differentiation. These pathways support SLUG's role in promoting EMT by modulating its transcriptional activity. SLUG often interacts with proteins such as TWIST1 and ZEB1 which synergistically act to downregulate epithelial markers and upregulate mesenchymal markers. This synergy highlights SLUG's significant role in facilitating changes in cellular architecture and function.

SLUG shows a strong connection with the progression of cancers and fibrosis. It contributes to cancer metastasis through its competence in inducing EMT enabling tumor cells to invade and establish secondary tumors. In breast cancer SLUG correlates with increased invasiveness and poor prognosis. In fibrosis SLUG promotes tissue scarring by facilitating fibroblast activation and extracellular matrix deposition. SLUG has interactions with other proteins such as E-Cadherin where its repressing activity on E-Cadherin contributes to the disruption of cell-cell adhesion a hallmark of metastatic cells.
style
left-column
style
left-column

Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:
style
left-column

Target data

Transcriptional repressor that modulates both activator-dependent and basal transcription. Involved in the generation and migration of neural crest cells. Plays a role in mediating RAF1-induced transcriptional repression of the TJ protein, occludin (OCLN) and subsequent oncogenic transformation of epithelial cells (By similarity). Represses BRCA2 expression by binding to its E2-box-containing silencer and recruiting CTBP1 and HDAC1 in breast cells. In epidermal keratinocytes, binds to the E-box in ITGA3 promoter and represses its transcription. Involved in the regulation of ITGB1 and ITGB4 expression and cell adhesion and proliferation in epidermal keratinocytes. Binds to E-box2 domain of BSG and activates its expression during TGFB1-induced epithelial-mesenchymal transition (EMT) in hepatocytes. Represses E-Cadherin/CDH1 transcription via E-box elements. Involved in osteoblast maturation. Binds to RUNX2 and SOC9 promoters and may act as a positive and negative transcription regulator, respectively, in osteoblasts. Binds to CXCL12 promoter via E-box regions in mesenchymal stem cells and osteoblasts. Plays an essential role in TWIST1-induced EMT and its ability to promote invasion and metastasis.
See full target information SNAI2
style
left-column

Publications (206)

Recent publications for all applications. Explore the full list and refine your search

iScience 28:112988 PubMed41054545

2025

Multiregional single-cell transcriptomics reveals an association between partial EMT and immunosuppressive states in oral squamous cell carcinoma.

Applications

Unspecified application

Species

Unspecified reactive species

Seunghoon Kim,Hyun Jung Kee,Dahee Kim,Jinho Jang,Hyoung-Oh Jeong,Nam Suk Sim,Mischa Selig,Jana Ihlow,Livius Penter,Taejoo Hwang,David Whee-Young Choi,Kyoung Jun Lee,Jaewoong Lee,Young Min Park,Semin Lee,Yoon Woo Koh

The Journal of pathology 266:481-494 PubMed40600748

2025

Involvement of KEAP1/NRF2 pathway in non-BRAF mutated squamous cell carcinoma of the thyroid.

Applications

Unspecified application

Species

Unspecified reactive species

Elin Schoultz,Jakob Dahlberg,Lisa M Nilsson,Jozefina J Dzanan,Therese Carlsson,Niklas Dahr,Ellinor Andersson,Ghayeb Muhammad,Andreas Muth,Erik Elias,Henrik Fagman,Volkan I Sayin,Jonas A Nilsson,Mikael Nilsson

Journal of translational medicine 23:524 PubMed40346622

2025

Knockdown of eIF3a alleviates pulmonary arterial hypertension by inhibiting endothelial-to-mesenchymal transition via TGFβ1/SMAD pathway.

Applications

Unspecified application

Species

Unspecified reactive species

Qiuhong Jiao,Xiufeng Xu,Longwu Xu,Yuying Wang,Shulan Pang,Jie Hao,Xiaohong Liu,Yudan Zhao,Wanpeng Qi,Limin Qin,Tao Huang,Jingtian Li,Tao Wang

Oncology letters 29:67 PubMed39619421

2024

Olaparib promotes FABP4 expression and reduces antitumor effect in ovarian cancer cells with a mutation.

Applications

Unspecified application

Species

Unspecified reactive species

Wei Huang,Hongxue Meng,Ye Xu,Lan Huang,Ge Lou

The World Allergy Organization journal 17:100963 PubMed39295955

2024

Sphk1 regulates HMGB1 via HDAC4 and mediates epithelial pyroptosis in allergic rhinitis.

Applications

Unspecified application

Species

Unspecified reactive species

Wei Huang,Xi Chen,Zizhen Liu,Changwu Li,Xin Wei,Jiabin Zhan,Quan Qiu,Jing Zheng

Cancer letters 598:217110 PubMed38986733

2024

PP2A B55α inhibits epithelial-mesenchymal transition via regulation of Slug expression in non-small cell lung cancer.

Applications

Unspecified application

Species

Unspecified reactive species

Deepika Singh,Zhaojun Qiu,Spehar M Jonathan,Pengyan Fa,Hannah Thomas,Chandra Bhushan Prasad,Shurui Cai,Jing J Wang,Chunhong Yan,Xiaoli Zhang,Monica Venere,Zaibo Li,Steven T Sizemore,Qi-En Wang,Junran Zhang

Scientific reports 14:13523 PubMed38866755

2024

GD2 and its biosynthetic enzyme GD3 synthase promote tumorigenesis in prostate cancer by regulating cancer stem cell behavior.

Applications

Unspecified application

Species

Unspecified reactive species

Aaqib M Bhat,Bhopal C Mohapatra,Haitao Luan,Insha Mushtaq,Sukanya Chakraborty,Siddhartha Kumar,Wangbin Wu,Ben Nolan,Samikshan Dutta,Matthew D Storck,Micah Schott,Jane L Meza,Subodh M Lele,Ming-Fong Lin,Leah M Cook,Eva Corey,Colm Morrissey,Donald W Coulter,M Jordan Rowley,Amarnath Natarajan,Kaustubh Datta,Vimla Band,Hamid Band

Molecular and cellular biology 44:178-193 PubMed38767243

2024

TCF12 Transcriptionally Activates SPHK1 to Induce Osteosarcoma Angiogenesis by Promoting the S1P/S1PR4/STAT3 Axis.

Applications

Unspecified application

Species

Unspecified reactive species

Wo Li,Jitong Liu,Ting Cai,Xia Hu

Journal of experimental & clinical cancer research : CR 43:103 PubMed38570866

2024

Mesothelin promotes brain metastasis of non-small cell lung cancer by activating MET.

Applications

Unspecified application

Species

Unspecified reactive species

Shengkai Xia,Wenzhe Duan,Mingxin Xu,Mengqi Li,Mengyi Tang,Song Wei,Manqing Lin,Encheng Li,Wenwen Liu,Qi Wang

Cancer cell international 24:121 PubMed38555451

2024

β-hydroxybutyrate inhibits malignant phenotypes of prostate cancer cells through β-hydroxybutyrylation of indoleacetamide-N-methyltransferase.

Applications

Unspecified application

Species

Unspecified reactive species

Yifan Zhang,Yunlong Li
View all publications
style
left-column
style
left-column
style
right-column

Alternative Products

Primary Antibodies

AB183760

Anti-SLUG antibody

Western blot - Anti-SLUG antibody (AB183760)

  • 0
  • 0
Primary Antibodies

AB180714

Anti-SNAIL + SLUG antibody

Western blot - Anti-SNAIL + SLUG antibody (AB180714)

  • 4
  • 6
Primary Antibodies

AB106077

Anti-SLUG antibody

Western blot - Anti-SLUG antibody (AB106077)

  • 4
  • 4

Complementary Products

Primary Antibodies

AB18203

Anti-N Cadherin antibody - Intercellular Junction Marker

BOND RX™ Validated|Lab Essentials

Western blot - Anti-N Cadherin antibody - Intercellular Junction Marker (AB18203)

  • 4
  • 48
Primary Antibodies

AB92547

Anti-Vimentin antibody [EPR3776] - Cytoskeleton Marker

BOND RX™ Validated|KO Validated|RabMAb|Recombinant|Lab Essentials|20ul selling size|Advanced Validation

Multiplex immunohistochemistry - Anti-Vimentin antibody [EPR3776] - Cytoskeleton Marker (AB92547)

  • 5
  • 92
Primary Antibodies

AB9485

Anti-GAPDH antibody - Loading Control

BOND RX™ Validated|Lab Essentials

Immunocytochemistry/ Immunofluorescence - Anti-GAPDH antibody - Loading Control (AB9485)

  • 5
  • 88
Secondary Antibodies

AB150077

Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488)

Immunocytochemistry/ Immunofluorescence - Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) (AB150077)

  • 5
  • 20

Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.
For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com