Anti-Slug antibody [BLR139H] - BSA free

• IP

Supplier Data

Immunoprecipitation - Anti-Slug antibody [BLR139H] - BSA free (AB314086)

Samples : Whole cell lysate (1.0 mg per IP reaction; 20% of IP loaded) from MDA-MB-435 cells prepared using NETN lysis buffer. Antibodies : Rabbit anti-Slug recombinant monoclonal antibody [BLR139H] used for IP at 20 µl/mg lysate. Slug was also immunoprecipitated by rabbit anti-Slug antibody. For blotting immunoprecipitated SLUG, A700-139 was used at 1 : 1000. Chemiluminescence with an exposure time of 30 seconds.

false

• WB

Supplier Data

Western blot - Anti-Slug antibody [BLR139H] - BSA free (AB314086)

Samples : Whole cell lysate (50 µg) from MCF-7, HeLa, HEK293T, MDAMB-435, Jurkat, MDA-MB-231, Hep-G2, and K-562 cells prepared using NETN lysis buffer. Antibody : Rabbit antiSlug recombinant monoclonal antibody [BLR139H] used at 1 : 1000. Secondary : HRPconjugated goat anti-rabbit IgG. Chemiluminescence with an exposure time of 3 seconds. Lower Panel : Rabbit anti-COPB2 antibody

All lanes:

Western blot - Anti-Slug antibody [BLR139H] - BSA free (ab314086) at 1/1000 dilution

Lane 1:

MCF-7 Whole cell lysate at 50 µg

Lane 2:

HeLa Whole cell lysate at 50 µg

Lane 3:

HEK293T Whole cell lysate at 50 µg

Lane 4:

MDAMB-435 Whole cell lysate at 50 µg

Lane 5:

Jurkat Whole cell lysate at 50 µg

Lane 6:

MDA-MB-231 Whole cell lysate at 50 µg

Lane 7:

Hep-G2 Whole cell lysate at 50 µg

Lane 8:

K-562 Whole cell lysate at 50 µg

Secondary

All lanes:

HRP-conjugated goat anti-rabbit IgG

false