AB51772

Anti-SLUG antibody [mAbcam51772]

Name: Anti-SLUG antibody [mAbcam51772] (ab51772) | Abcam
Brand: Abcam Limited
SKU: AB51772
Price: 450 USD
Availability: InStock
Rating: 3 (9 reviews)

(9 Reviews)

(57 Publications)

Mouse Monoclonal SLUG antibody. Suitable for WB and reacts with Mouse, Human samples. Cited in 57 publications.

View Alternative Names

SLUG, SLUGH, SNAI2, Zinc finger protein SNAI2, Neural crest transcription factor Slug, Protein snail homolog 2

3 Images

Project

Western blot - Anti-SLUG antibody [mAbcam51772] (AB51772)

All lanes:

Western blot - Anti-SLUG antibody [mAbcam51772] (ab51772) at 1 µg/mL

Lane 1:

Skin tissue lysate from Slug heterozygous mouse

Lane 2:

Skin tissue lysate from Slug knockout mouse

Predicted band size: 29 kDa

false

Project

Western blot - Anti-SLUG antibody [mAbcam51772] (AB51772)

All lanes:

Western blot - Anti-SLUG antibody [mAbcam51772] (ab51772) at 5 µg/mL

All lanes:

Western blot - Mouse skin tissue lysate - total protein (<a href='/en-us/products/tissue-lysates/mouse-skin-tissue-lysate-total-protein-ab4025'>ab4025</a>) at 10 µg

Secondary

All lanes:

Goat polyclonal to Mouse IgG - H&L - Pre-Adsorbed (HRP) at 1/3000 dilution

Predicted band size: 29 kDa

Observed band size: 30 kDa,34 kDa,60 kDa

false

Exposure time: 10min

Unknown

Western blot - Anti-SLUG antibody [mAbcam51772] (AB51772)

All lanes:

Western blot - Anti-SLUG antibody [mAbcam51772] (ab51772)

Lane 1:

Untransfected MCF7 cells

Lane 2:

MCF7 cells transfected with SLUG

Lane 3:

MCF7 cells transfected with Snail

Predicted band size: 29 kDa

Observed band size: 30 kDa

false

Key facts

Host species

Mouse

Clonality

Monoclonal

Clone number

mAbcam51772

Isotype

IgG1

Carrier free

Reacts with

Mouse, Human

Applications

applications

Immunogen

The exact immunogen used to generate this antibody is proprietary information.

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1-5 µg/mL", "WB-species-notes": "<p></p>" }, "Mouse": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1-5 µg/mL", "WB-species-notes": "<p></p>" }, "Chicken": { "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "" }, "Cow": { "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "" }, "Pig": { "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "" }, "Xenopus laevis": { "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "" } } }

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Product details

Want a custom formulation?
This antibody clone is manufactured by Abcam. If you require a custom buffer formulation or conjugation for your experiments, please contact orders@abcam.com

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Properties and storage information

Form

Liquid

Purification technique

Affinity purification

Storage buffer

pH: 7.4 Preservative: 0.02% Sodium azide Constituents: PBS, 6.97% L-Arginine

Shipped at conditions

Blue Ice

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

-20°C

Aliquoting information

Upon delivery aliquot

Storage information

Avoid freeze / thaw cycle

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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

SLUG also known as SNAI2 is a zinc finger transcription factor that plays a mechanical role in the regulation of genes implicated in cell differentiation and development. With an approximate mass of 29 kDa SLUG is expressed in various tissues particularly in the neural crest and epithelial-cell precursors. It acts as a repressor by binding to E-box motifs in the promoter regions of its target genes. SLUG functions in coordination with other cofactors to modulate gene expression that influences diverse biological processes including epithelial-mesenchymal transition (EMT).

Biological function summary

SLUG influences cell motility and invasion by controlling EMT a process critical for development and cancer metastasis. SLUG functions as a part of the Snail family of transcription factors and collaborates with other EMT-related molecules. It influences the expression of genes that maintain the epithelial phenotype facilitating the switch to a mesenchymal state required for increased cell mobility. Through these actions SLUG participates in the dynamic remodeling of tissues and is an important player during embryonic development and in certain pathological conditions.

Pathways

Researchers have associated SLUG with the TGF-beta and Wnt signaling pathways both essential for cell growth and differentiation. These pathways support SLUG's role in promoting EMT by modulating its transcriptional activity. SLUG often interacts with proteins such as TWIST1 and ZEB1 which synergistically act to downregulate epithelial markers and upregulate mesenchymal markers. This synergy highlights SLUG's significant role in facilitating changes in cellular architecture and function.

SLUG shows a strong connection with the progression of cancers and fibrosis. It contributes to cancer metastasis through its competence in inducing EMT enabling tumor cells to invade and establish secondary tumors. In breast cancer SLUG correlates with increased invasiveness and poor prognosis. In fibrosis SLUG promotes tissue scarring by facilitating fibroblast activation and extracellular matrix deposition. SLUG has interactions with other proteins such as E-Cadherin where its repressing activity on E-Cadherin contributes to the disruption of cell-cell adhesion a hallmark of metastatic cells.

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

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Target data

Transcriptional repressor that modulates both activator-dependent and basal transcription. Involved in the generation and migration of neural crest cells. Plays a role in mediating RAF1-induced transcriptional repression of the TJ protein, occludin (OCLN) and subsequent oncogenic transformation of epithelial cells (By similarity). Represses BRCA2 expression by binding to its E2-box-containing silencer and recruiting CTBP1 and HDAC1 in breast cells. In epidermal keratinocytes, binds to the E-box in ITGA3 promoter and represses its transcription. Involved in the regulation of ITGB1 and ITGB4 expression and cell adhesion and proliferation in epidermal keratinocytes. Binds to E-box2 domain of BSG and activates its expression during TGFB1-induced epithelial-mesenchymal transition (EMT) in hepatocytes. Represses E-Cadherin/CDH1 transcription via E-box elements. Involved in osteoblast maturation. Binds to RUNX2 and SOC9 promoters and may act as a positive and negative transcription regulator, respectively, in osteoblasts. Binds to CXCL12 promoter via E-box regions in mesenchymal stem cells and osteoblasts. Plays an essential role in TWIST1-induced EMT and its ability to promote invasion and metastasis.

See full target information SNAI2

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Publications (57)

Recent publications for all applications. Explore the full list and refine your search

Cell death & disease 16:1 PubMed39753531

2025

Endothelial-Ercc1 DNA repair deficiency provokes blood-brain barrier dysfunction.

Applications

Unspecified application

Species

Unspecified reactive species

Cathrin E Hansen,Davide Vacondio,Lennart van der Molen,Annika A Jüttner,Wing Ka Fung,Manon Karsten,Bert van Het Hof,Ruud D Fontijn,Gijs Kooij,Maarten E Witte,Anton J M Roks,Helga E de Vries,Inge Mulder,Nienke M de Wit

The FEBS journal 292:168-190 PubMed39578310

2024

Regulation of the HMGA2-SNAI2/CXCR4 axis in atherosclerosis and retinal neovascularization: new therapeutic insights.

Applications

Unspecified application

Species

Unspecified reactive species

Jianan Li,Zhuxi Liu,Chunyong Yu,Weilong Song,Xiaobin Zhang,Guobiao Liang

Cancers 16: PubMed38927986

2024

SMARCD3 Overexpression Promotes Epithelial-Mesenchymal Transition in Gastric Cancer.

Applications

Unspecified application

Species

Unspecified reactive species

Sun Yi Park,Ji-Ho Park,Jung Wook Yang,Eun-Jung Jung,Young-Tae Ju,Chi-Young Jeong,Ju-Yeon Kim,Taejin Park,Tae-Han Kim,Miyeong Park,Young-Joon Lee,Sang-Ho Jeong

Technology in cancer research & treatment 23:15330338231187254 PubMed38303513

2024

HTATIP2 Overexpression was Associated With a Good Prognosis in Gastric Cancer.

Applications

Unspecified application

Species

Unspecified reactive species

Sun Yi Park,Ji-Ho Park,Jung Wook Yang,Eun-Jung Jung,Young-Tae Ju,Chi-Young Jeong,Ju-Yeon Kim,Taejin Park,Miyeong Park,Young-Joon Lee,Sang-Ho Jeong

Annals of surgical treatment and research 105:385-395 PubMed38076599

2023

Exploring the role of copine 1 in human colorectal cancer: investigating its association with tumorigenesis and metastasis.

Applications

Unspecified application

Species

Unspecified reactive species

Jin-Kwon Lee,Seung-Jun Lee,Young-Sool Hah,Yeong-Ho Ji,Young-Tae Ju,Young-Joon Lee,Chi-Young Jeong,Ju-Yeon Kim,Ji-Ho Park,Jae-Myung Kim,Jin-Kyu Cho,Han-Gil Kim,Seung-Jin Kwag

American journal of translational research 15:2426-2442 PubMed37193148

2023

Long non-coding RNA LINC00707, a prognostic marker, regulates cell proliferation, apoptosis, and EMT in esophageal squamous cell carcinoma.

Applications

Unspecified application

Species

Unspecified reactive species

Na Gao,Guozhong Jiang,Zhengfan Gao,Mengfei Cui,Jing Li,Hongtao Liu,Tianli Fan

Open medicine (Warsaw, Poland) 18:20220593 PubMed36712590

2023

Up-regulation of collagen type V alpha 2 () promotes malignant phenotypes in gastric cancer cell via inducing epithelial-mesenchymal transition (EMT).

Applications

Unspecified application

Species

Unspecified reactive species

Yanfeng Jin,Xinyan Song,Xuankai Sun,Yan Ding

Oxidative medicine and cellular longevity 2022:1030238 PubMed36589681

2022

Inositol Alleviates Pulmonary Fibrosis by Promoting Autophagy via Inhibiting the HIF-1-SLUG Axis in Acute Respiratory Distress Syndrome.

Applications

Unspecified application

Species

Unspecified reactive species

Yufeng Liang,Yingyi Xu,Bingtai Lu,Yuanming Huang,Shuman Xu,Junjie Xie,Ming Liu,Di Che,Liuheyi Ma,Jianping Tao,Jie Hong,Jianhui Zhang,Xun Situ,XinXu Ou,Lihe Chen,Yang Li,Lihong Zhang,Zhiyuan Wu

Disease markers 2022:1217918 PubMed35978886

2022

TAB2 Promotes the Biological Functions of Head and Neck Squamous Cell Carcinoma Cells via EMT and PI3K Pathway.

Applications

Unspecified application

Species

Unspecified reactive species

Huijuan Liu,Hui Zhang,Haidong Fan,Su Tang,Junquan Weng

Journal of oncology 2022:4202439 PubMed35847369

2022

No Effect on Tumorigenesis in MG63 Cells Induced by Co-Cultured Mesenchymal Stem Cells.

Applications

Unspecified application

Species

Unspecified reactive species

Xin Xing,Yue-Hua Hu,Yan Wang,Yi Shao,Min Zou

View all publications

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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.

For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com

Anti-SLUG antibody [mAbcam51772]

Western blot - Anti-SLUG antibody [mAbcam51772] (AB51772)

Western blot - Anti-SLUG antibody [mAbcam51772] (AB51772)

Western blot - Anti-SLUG antibody [mAbcam51772] (AB51772)

Key facts

Host species

Clonality

Clone number

Isotype

Carrier free

Reacts with

Applications

Immunogen

Reactivity data

Product details

Properties and storage information

Form

Purification technique

Storage buffer

Shipped at conditions

Appropriate short-term storage conditions

Appropriate long-term storage conditions

Aliquoting information

Storage information

Supplementary information

Biological function summary

Pathways

Product protocols

Target data

Publications (57)

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