AB86594

Anti-Sly1 antibody

Name: Anti-Sly1 antibody (ab86594) | Abcam
Brand: Abcam Limited
SKU: AB86594

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(2 Publications)

Rabbit Polyclonal Sly1 antibody. Suitable for IP, WB and reacts with Human, Mouse samples. Cited in 2 publications. Immunogen corresponding to Synthetic Peptide within Human SCFD1 aa 300-350.

View Alternative Names

C14orf163, KIAA0917, STXBP1L2, FKSG23, SCFD1, Sec1 family domain-containing protein 1, SLY1 homolog, Syntaxin-binding protein 1-like 2, Sly1p

2 Images

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Immunoprecipitation - Anti-Sly1 antibody (AB86594)

1mg whole cell lysate from Hela cells was immunoprecipited using 3ug ab86594. 20% of IP was loaded per lane, and probed with ab86549 at 1ug/ml (lane 1) or with a control IgG (lane 2).
Detection : chemiluminescence with an exposure time of 30 seconds.

All lanes:

Immunoprecipitation - Anti-Sly1 antibody (ab86594)

Predicted band size: 72 kDa

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Western blot - Anti-Sly1 antibody (AB86594)

All lanes:

Western blot - Anti-Sly1 antibody (ab86594) at 0.04 µg/mL

Lane 1:

Whole cell lysate from HeLa cells at 50 µg

Lane 2:

Whole cell lysate from HeLa cells at 15 µg

Lane 3:

Whole cell lysate from HeLa cells at 5 µg

Lane 4:

Whole cell lysate from 293T cells at 50 µg

Lane 5:

Whole cell lysate from NIH3T3 cells at 50 µg

Predicted band size: 72 kDa

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Exposure time: 3min

Key facts

Host species

Rabbit

Clonality

Polyclonal

Isotype

IgG

Carrier free

Reacts with

Mouse, Human

Applications

WB, IP

applications

Immunogen

Synthetic Peptide within Human SCFD1 aa 300-350. The exact immunogen used to generate this antibody is proprietary information.

Q8WVM8

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "IP" : {"fullname" : "Immunoprecipitation", "shortname":"IP"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "IP-species-checked": "testedAndGuaranteed", "IP-species-dilution-info": "2-5 µg/mg of lysate", "IP-species-notes": "", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/2000 - 1/10000", "WB-species-notes": "" }, "Mouse": { "IP-species-checked": "guaranteed", "IP-species-dilution-info": "", "IP-species-notes": "", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/2000 - 1/10000", "WB-species-notes": "" }, "Rat": { "IP-species-checked": "predicted", "IP-species-dilution-info": "", "IP-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "" }, "Chimpanzee": { "IP-species-checked": "predicted", "IP-species-dilution-info": "", "IP-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "" }, "Cow": { "IP-species-checked": "predicted", "IP-species-dilution-info": "", "IP-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "" }, "Dog": { "IP-species-checked": "predicted", "IP-species-dilution-info": "", "IP-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "" }, "Elephant": { "IP-species-checked": "predicted", "IP-species-dilution-info": "", "IP-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "" }, "Ferret": { "IP-species-checked": "predicted", "IP-species-dilution-info": "", "IP-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "" }, "Gorilla": { "IP-species-checked": "predicted", "IP-species-dilution-info": "", "IP-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "" }, "Horse": { "IP-species-checked": "predicted", "IP-species-dilution-info": "", "IP-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "" }, "Orangutan": { "IP-species-checked": "predicted", "IP-species-dilution-info": "", "IP-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "" }, "Pig": { "IP-species-checked": "predicted", "IP-species-dilution-info": "", "IP-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "" }, "Rabbit": { "IP-species-checked": "predicted", "IP-species-dilution-info": "", "IP-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "" }, "Rhesus monkey": { "IP-species-checked": "predicted", "IP-species-dilution-info": "", "IP-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "" } } }

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Properties and storage information

Form

Liquid

Purification technique

Affinity purification Immunogen

Storage buffer

pH: 6.8 - 7.4 Preservative: 0.09% Sodium azide Constituents: Tris buffered saline, 0.1% BSA

Shipped at conditions

Blue Ice

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

+4°C

Aliquoting information

Upon delivery aliquot

Storage information

Avoid freeze / thaw cycle

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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

Sly1 also known as suppressor of Ypt1 homolog 1 functions as a protein involved in vesicle fusion. It has a molecular mass of approximately 67 kDa and serves as an important element in vesicular trafficking processes. Expressed in various tissues Sly1 primarily resides in the cytoplasm and the Golgi apparatus. It facilitates the fusion of transport vesicles to compartment membranes by binding to SNARE proteins therefore playing a role in maintaining normal cellular transport operations.

Biological function summary

Sly1 influences cellular transport by being part of the SM (Sec1/Munc18) protein family. It regulates SNARE complex assembly ensuring accurate vesicle docking and fusion events. Sly1 interacts with different t-SNAREs such as Syntaxin 5 modulating their functions and energy coupling during vesicle fusion. Proper action of Sly1 is necessary for maintaining cellular organization and intracellular communication influencing the efficiency of endocytotic and exocytotic pathways.

Pathways

Sly1 participates actively within the vesicle transport mechanisms linked by SNARE proteins. It plays a fundamental role in the secretory pathway affecting protein and lipid trafficking. Interacting with proteins such as Ypt1 a small GTPase Sly1 targets vesicle transport to the correct membranes for fusion. These interactions are important for the functionality of Golgi-mediated vesicle transport and signal transductions as well as for maintaining cellular homeostasis.

Impairments in Sly1 expression or function relate to neurodegenerative diseases such as Alzheimer's disease. These conditions associate with defects in vesicular trafficking pathways as evidenced by altered Sly1 interactions with proteins like Syntaxin 5. Such interruptions can cause misprocessing and accumulation of proteins within neurons. Another disorder linked to Sly1 is developmental brain disorder where disruptions in vesicular fusion contribute to improper neuronal connections.

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:

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Target data

Plays a role in SNARE-pin assembly and Golgi-to-ER retrograde transport via its interaction with COG4. Involved in vesicular transport between the endoplasmic reticulum and the Golgi (By similarity).

See full target information SCFD1

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Publications (2)

Recent publications for all applications. Explore the full list and refine your search

Molecular biology of the cell 35:ar20 PubMed38134218

2023

Recruitment of PI4KIIIβ to the Golgi by ACBD3 is dependent on an upstream pathway of a SNARE complex and golgins.

Applications

Unspecified application

Species

Unspecified reactive species

Danièle Stalder,Igor Yakunin,Conceição Pereira,Jessica Eden,David C Gershlick

Human molecular genetics 21:586-608 PubMed22038835

2011

Loss of ceramide synthase 3 causes lethal skin barrier disruption.

Applications

Unspecified application

Species

Unspecified reactive species

Richard Jennemann,Mariona Rabionet,Karin Gorgas,Sharon Epstein,Alexander Dalpke,Ulrike Rothermel,Aline Bayerle,Franciscus van der Hoeven,Silke Imgrund,Joachim Kirsch,Walter Nickel,Klaus Willecke,Howard Riezman,Hermann-Josef Gröne,Roger Sandhoff

View all publications

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Product promise

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For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

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Anti-Sly1 antibody

Immunoprecipitation - Anti-Sly1 antibody (AB86594)

Western blot - Anti-Sly1 antibody (AB86594)

Key facts

Host species

Clonality

Isotype

Carrier free

Reacts with

Applications

Immunogen

Complementary Products

Primary Antibodies

Primary Antibodies

Secondary Antibodies

Primary Antibodies

Reactivity data

Properties and storage information

Form

Purification technique

Storage buffer

Shipped at conditions

Appropriate short-term storage conditions

Appropriate long-term storage conditions

Aliquoting information

Storage information

Supplementary information

Biological function summary

Pathways

Product protocols

Target data

Publications (2)

Product promise