AB8115

Anti-Smac/Diablo antibody

Name: Anti-Smac/Diablo antibody (ab8115) | Abcam
Brand: Abcam Limited
SKU: AB8115
Price: 535 USD
Availability: InStock
Rating: 4 (1 reviews)

(1 Review)

(11 Publications)

Rabbit Polyclonal Smac/Diablo antibody. Suitable for WB and reacts with Mouse, Rat samples. Cited in 11 publications. Immunogen corresponding to Synthetic Peptide within Mouse Diablo aa 200 to C-terminus.

View Alternative Names

Smac, Diablo, Diablo IAP-binding mitochondrial protein, Direct IAP-binding protein with low pI, Second mitochondria-derived activator of caspase

1 Images

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Western blot - Anti-Smac/Diablo antibody (AB8115)

All lanes:

Western blot - Anti-Smac/Diablo antibody (ab8115) at 1 µg/mL

Lane 1:

Mouse heart tissue lysate with absence of blocking peptide

Lane 2:

Mouse heart tissue lysate with presence of blocking peptide

Lane 3:

Rat heart tissue lysate with absence of blocking peptide

Predicted band size: 27 kDa

Observed band size: 25 kDa

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Key facts

Host species

Rabbit

Clonality

Polyclonal

Isotype

IgG

Carrier free

Reacts with

Rat, Mouse

Applications

applications

Immunogen

Synthetic Peptide within Mouse Diablo aa 200 to C-terminus. The exact immunogen used to generate this antibody is proprietary information.

Q9JIQ3

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Mouse": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "", "WB-species-notes": "<p></p>" }, "Rat": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "", "WB-species-notes": "<p></p>" } } }

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Properties and storage information

Form

Liquid

Purification technique

Affinity purification DEAE-C

Storage buffer

pH: 7.2 Preservative: 0.02% Sodium azide

Shipped at conditions

Blue Ice

Appropriate short-term storage duration

1-2 weeks

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

-20°C

Aliquoting information

Upon delivery aliquot

Storage information

Avoid freeze / thaw cycle|Stable for 12 months at -20°C

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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

Smac/Diablo is a mitochondrial protein that promotes apoptosis by interfering with inhibitor of apoptosis proteins (IAPs). The protein also known as Second Mitochondria-derived Activator of Caspases weighs approximately 27 kDa. Researchers detect Smac/Diablo in various tissues with higher expression levels in organs like the heart and brain. This protein's expression also varies depending on the cell's state and external stimuli.

Biological function summary

Smac/Diablo plays a significant role in programmed cell death by binding to IAPs and negating their inhibition of caspases the key executors of apoptosis. Smac/Diablo releases from mitochondria into the cytosol when apoptotic signals activate. It does not form complexes but interacts with IAPs facilitating the activation of caspases and enhancing the apoptotic response. Its interaction with IAPs highlights its important function in apoptosis regulation.

Pathways

Smac/Diablo integrates into the mitochondrial apoptosis pathway contributing to the intrinsic pathway of apoptosis. It closely interacts with proteins such as caspases and IAPs. This integration is important for apoptosis regulation linking mitochondrial outer membrane permeabilization with the activation of caspases. Additionally the protein engages in the cytochrome c pathway promoting apoptosis by restricting IAPs and aiding in the release of cytochrome c important for apoptosis progression.

Researchers associate Smac/Diablo with cancer and neurodegenerative diseases like Alzheimer's disease. In cancer elevated Smac/Diablo levels can result in increased apoptotic death of cancerous cells potentially serving as a target for therapy. It also interacts with proteins like XIAP in these disease contexts. In neurodegenerative disorders dysregulation of apoptosis pathways involving Smac/Diablo might contribute to excessive neuronal cell death. Understanding Smac/Diablo's role in these diseases provides insights into possible therapeutic interventions.

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

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Target data

Promotes apoptosis by activating caspases in the cytochrome c/Apaf-1/caspase-9 pathway. Acts by opposing the inhibitory activity of inhibitor of apoptosis proteins (IAP). Inhibits the activity of BIRC6/BRUCE by inhibiting its binding to caspases (By similarity).

See full target information Diablo

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Publications (11)

Recent publications for all applications. Explore the full list and refine your search

International journal of molecular sciences 23: PubMed35806364

2022

Tumor-Suppressor Role of the α1-Na/K-ATPase Signalosome in NASH Related Hepatocellular Carcinoma.

Applications

Unspecified application

Species

Unspecified reactive species

Utibe-Abasi S Udoh,Moumita Banerjee,Pradeep K Rajan,Juan D Sanabria,Gary Smith,Mathew Schade,Jacqueline A Sanabria,Yuto Nakafuku,Komal Sodhi,Sandrine V Pierre,Joseph I Shapiro,Juan R Sanabria

Neural regeneration research 16:1813-1820 PubMed33510088

2021

Mitochonic acid 5 regulates mitofusin 2 to protect microglia.

Applications

Unspecified application

Species

Unspecified reactive species

Jian Tan,Shuang-Xi Chen,Qing-Yun Lei,Shan-Qing Yi,Na Wu,Yi-Lin Wang,Zi-Jian Xiao,Heng Wu

IUBMB life 73:492-510 PubMed33179373

2020

Mitochondria and nucleus cross-talk: Signaling in metabolism, apoptosis, and differentiation, and function in cancer.

Applications

Unspecified application

Species

Unspecified reactive species

Anna Shteinfer-Kuzmine,Ankit Verma,Tasleem Arif,Or Aizenberg,Avijit Paul,Varda Shoshan-Barmaz

Journal of the American Heart Association 9:e014814 PubMed32301368

2020

Interleukin-22 Directly Activates Myocardial STAT3 (Signal Transducer and Activator of Transcription-3) Signaling Pathway and Prevents Myocardial Ischemia Reperfusion Injury.

Applications

Unspecified application

Species

Unspecified reactive species

Jinya Takahashi,Mai Yamamoto,Hideo Yasukawa,Shoichiro Nohara,Takanobu Nagata,Koutatsu Shimozono,Toshiyuki Yanai,Tomoko Sasaki,Kota Okabe,Tatsuhiro Shibata,Kazutoshi Mawatari,Tatsuyuki Kakuma,Hiroki Aoki,Yoshihiro Fukumoto

Oncology reports 40:1614-1620 PubMed30015942

2018

Apoptotic effect of pyrroloquinoline quinone on chondrosarcoma cells through activation of the mitochondrial caspase‑dependent and caspase‑independent pathways.

Applications

Unspecified application

Species

Unspecified reactive species

Ronghuan Wu,Jun Pan,Miaoda Shen,Chunyang Xing

Molecular metabolism 13:10-23 PubMed29780003

2018

A disease-associated Aifm1 variant induces severe myopathy in knockin mice.

Applications

Unspecified application

Species

Unspecified reactive species

Lena Wischhof,Anna Gioran,Dagmar Sonntag-Bensch,Antonia Piazzesi,Miriam Stork,Pierluigi Nicotera,Daniele Bano

Oncology letters 15:7707-7715 PubMed29740490

2018

Silencing Livin improved the sensitivity of colon cancer cells to 5-fluorouracil by regulating crosstalk between apoptosis and autophagy.

Applications

Unspecified application

Species

Unspecified reactive species

Shuai Liu,Xin Li,Qing Li,Hongjun Liu,Yulong Shi,Hongqing Zhuo,Chensheng Li,Huijuan Zhu

Mitochondrion 29:45-52 PubMed27181046

2016

Analysis of Mitochondrial haemoglobin in Parkinson's disease brain.

Applications

Species

Unspecified reactive species

Freya Shephard,Oliver Greville-Heygate,Susan Liddell,Richard Emes,Lisa Chakrabarti

PloS one 11:e0148500 PubMed27078856

2016

Novel Biomarker Proteins in Chronic Lymphocytic Leukemia: Impact on Diagnosis, Prognosis and Treatment.

Applications

Unspecified application

Species

Human

Lee Admoni-Elisha,Itay Nakdimon,Anna Shteinfer,Tal Prezma,Tasleem Arif,Nir Arbel,Anna Melkov,Ori Zelichov,Itai Levi,Varda Shoshan-Barmatz

Mitochondrion 14:64-72 PubMed24333691

2013

A mitochondrial location for haemoglobins--dynamic distribution in ageing and Parkinson's disease.

Applications

Species

Mouse

Freya Shephard,Oliver Greville-Heygate,Oliver Marsh,Susan Anderson,Lisa Chakrabarti

View all publications

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