Anti-Smac/Diablo antibody [SMAC 17 1-87] (ab111893)

Mouse Monoclonal Smac/Diablo antibody. Suitable for WB, IHC-P, ICC/IF and reacts with Mouse, Human, Rat samples. Cited in 1 publication. Immunogen corresponding to Recombinant Full Length Protein corresponding to Human DIABLO.

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Images

Western blot - Anti-Smac/Diablo antibody [SMAC 17 1-87] (AB111893), expandable thumbnail

Publications

Key facts

Isotype: IgG1
Host species: Mouse
Storage buffer: Preservative: 0.05% Sodium azide
Constituents: 99% PBS, 0.1% BSA
Form: Liquid
Clonality: Monoclonal

Immunogen

Recombinant Full Length Protein corresponding to Human DIABLO. Database link Q9NR28

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Reactivity data

Select an application

Product promiseTestedExpectedPredictedNot recommended

	WB	IHC-P	ICC/IF
Human	Tested	Tested	Tested
Mouse	Tested	Tested	Expected
Rat	Expected	Expected	Tested

Tested
Tested

Species	Dilution info	Notes
Species Mouse, Human	Dilution info -	Notes -

Expected
Expected

Species	Dilution info	Notes
Species Rat	Dilution info Use at an assay dependent concentration.	Notes -

Tested
Tested

Species	Dilution info	Notes
Species Mouse	Dilution info 1/20.00000 - 1/200.00000	Notes Perform heat-mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
Species Human	Dilution info 1/20.00000 - 1/200.00000	Notes Perform heat-mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

Expected
Expected

Species	Dilution info	Notes
Species Rat	Dilution info Use at an assay dependent concentration.	Notes -

Tested
Tested

Species	Dilution info	Notes
Species Rat	Dilution info 1/20.00000 - 1/200.00000	Notes -
Species Human	Dilution info 1/20.00000 - 1/200.00000	Notes -

Expected
Expected

Species	Dilution info	Notes
Species Mouse	Dilution info Use at an assay dependent concentration.	Notes -

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Target data

See full target information DIABLO

Function

Promotes apoptosis by activating caspases in the cytochrome c/Apaf-1/caspase-9 pathway. Acts by opposing the inhibitory activity of inhibitor of apoptosis proteins (IAP). Inhibits the activity of BIRC6/BRUCE by inhibiting its binding to caspases (PubMed:15200957, PubMed:36758104, PubMed:36758105, PubMed:36758106). Isoform 3. Attenuates the stability and apoptosis-inhibiting activity of XIAP/BIRC4 by promoting XIAP/BIRC4 ubiquitination and degradation through the ubiquitin-proteasome pathway. Also disrupts XIAP/BIRC4 interacting with processed caspase-9 and promotes caspase-3 activation. Isoform 1. Defective in the capacity to down-regulate the XIAP/BIRC4 abundance.

Alternative names

SMAC, DIABLO, Diablo IAP-binding mitochondrial protein, Direct IAP-binding protein with low pI, Second mitochondria-derived activator of caspases

Key facts

Isotype: IgG1
Host species: Mouse
Storage buffer: Preservative: 0.05% Sodium azide
Constituents: 99% PBS, 0.1% BSA
Form: Liquid
Clonality: Monoclonal
Immunogen: Recombinant Full Length Protein corresponding to Human DIABLO. Database link Q9NR28
Clone number: SMAC 17 1-87
Purification technique: Affinity purification Protein G
Concentration

Storage

Shipped at conditions: Blue Ice
Appropriate short-term storage conditions: +4°C
Appropriate long-term storage conditions: -20°C
Aliquoting information: Upon delivery aliquot
Storage information: Avoid freeze / thaw cycle

Supplementary info

This supplementary information is collated from multiple sources and compiled automatically.

Activity summary

Smac/Diablo is a mitochondrial protein that promotes apoptosis by interfering with inhibitor of apoptosis proteins (IAPs). The protein also known as Second Mitochondria-derived Activator of Caspases weighs approximately 27 kDa. Researchers detect Smac/Diablo in various tissues with higher expression levels in organs like the heart and brain. This protein's expression also varies depending on the cell's state and external stimuli.

Biological function summary

Smac/Diablo plays a significant role in programmed cell death by binding to IAPs and negating their inhibition of caspases the key executors of apoptosis. Smac/Diablo releases from mitochondria into the cytosol when apoptotic signals activate. It does not form complexes but interacts with IAPs facilitating the activation of caspases and enhancing the apoptotic response. Its interaction with IAPs highlights its important function in apoptosis regulation.

Pathways

Smac/Diablo integrates into the mitochondrial apoptosis pathway contributing to the intrinsic pathway of apoptosis. It closely interacts with proteins such as caspases and IAPs. This integration is important for apoptosis regulation linking mitochondrial outer membrane permeabilization with the activation of caspases. Additionally the protein engages in the cytochrome c pathway promoting apoptosis by restricting IAPs and aiding in the release of cytochrome c important for apoptosis progression.

Associated diseases and disorders

Researchers associate Smac/Diablo with cancer and neurodegenerative diseases like Alzheimer's disease. In cancer elevated Smac/Diablo levels can result in increased apoptotic death of cancerous cells potentially serving as a target for therapy. It also interacts with proteins like XIAP in these disease contexts. In neurodegenerative disorders dysregulation of apoptosis pathways involving Smac/Diablo might contribute to excessive neuronal cell death. Understanding Smac/Diablo's role in these diseases provides insights into possible therapeutic interventions.

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8 product images

Western blot - Anti-Smac/Diablo antibody [SMAC 17 1-87] (ab111893)
All lanes: Western blot - Anti-Smac/Diablo antibody [SMAC 17 1-87] (ab111893) at 1 µg/mL
Lane 1: HeLa cell lysate at 30 µg
Lane 2: Jurkat cell lysate at 30 µg
Lane 3: MCF-7 cell lysate at 30 µg
Lane 4: Hep G2 cell lysate at 30 µg
Lane 5: NIH/3T3 cell lysate at 30 µg
Lane 6: PC-12 cell lysate at 30 µg
Secondary
All lanes: Goat anti-Mouse IgG (H+L) Superclonal™ Secondary Antibody, HRP conjugate at 1/4000 dilution
Predicted band size: 27 kDa
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Smac/Diablo antibody [SMAC 17 1-87] (ab111893)
Immunohistochemistry (paraffin-treated sections) analysis of human colon tissue labelling SMAC/Diablo with ab111893 at 1/100 dilution. Tissues were counterstained with hematoxylin and dehydrated with xylene and ethanol. Negative control (left) has no primary antibody. Heat mediated antigen retrieval performed with citrate buffer pH 6 before commencing with IHC staining protocol.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Smac/Diablo antibody [SMAC 17 1-87] (ab111893)
Immunohistochemistry (paraffin-treated sections) analysis of mouse testis tissue labelling SMAC/Diablo with ab111893 at 1/100 dilution. Tissues were counterstained with hematoxylin and dehydrated with xylene and ethanol. Negative control (left) has no primary antibody. Heat mediated antigen retrieval performed with citrate buffer pH 6 before commencing with IHC staining protocol.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Smac/Diablo antibody [SMAC 17 1-87] (ab111893)
Immunohistochemistry (paraffin-treated sections) analysis of human testis tissue labelling SMAC/Diablo with ab111893 at 1/100 dilution. Tissues were counterstained with hematoxylin and dehydrated with xylene and ethanol. Negative control (left) has no primary antibody. Heat mediated antigen retrieval performed with citrate buffer pH 6 before commencing with IHC staining protocol.
Immunocytochemistry/ Immunofluorescence - Anti-Smac/Diablo antibody [SMAC 17 1-87] (ab111893)
ab111893 labelling Smac / Diablo (green) in the cytoplasm of A431 cells by Immunocytochemistry/Immunofluorescence. Formalin-fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 5-10 minutes and blocked with 3% BSA-PBS for 30 minutes at room temperature. Cells were incubated with the primary antibody (1:100 in 3% BSA-PBS) overnight at 4 °C. A DyLight-conjugated anti-mouse IgG was used as the secondary antibody. Red (phalloidiin) - F-actin, Blue - nuclei. Images were taken at a magnification of 60x.
Immunocytochemistry/ Immunofluorescence - Anti-Smac/Diablo antibody [SMAC 17 1-87] (ab111893)
ab111893 labelling Smac / Diablo (green) in the cytoplasm of MCF-7 cells by Immunocytochemistry/Immunofluorescence. Formalin-fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 5-10 minutes and blocked with 3% BSA-PBS for 30 minutes at room temperature. Cells were incubated with the primary antibody (1:100 in 3% BSA-PBS) overnight at 4 °C. A DyLight-conjugated anti-mouse IgG was used as the secondary antibody. Red (phalloidiin) - F-actin, Blue - nuclei. Images were taken at a magnification of 60x.
Immunocytochemistry/ Immunofluorescence - Anti-Smac/Diablo antibody [SMAC 17 1-87] (ab111893)
ab111893 labelling Smac / Diablo (green) in the cytoplasm of PC12 cells by Immunocytochemistry/Immunofluorescence. Formalin-fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 5-10 minutes and blocked with 3% BSA-PBS for 30 minutes at room temperature. Cells were incubated with the primary antibody (1:100 in 3% BSA-PBS) overnight at 4 °C. A DyLight-conjugated anti-mouse IgG was used as the secondary antibody. Red (phalloidiin) - F-actin, Blue - nuclei. Images were taken at a magnification of 60x.
Western blot - Anti-Smac/Diablo antibody [SMAC 17 1-87] (ab111893)
All lanes: Western blot - Anti-Smac/Diablo antibody [SMAC 17 1-87] (ab111893) at 1/1000 dilution
Lane 1: 293 cell lysate at 25 µg
Lane 2: HeLa cell lysate at 25 µg
Lane 3: PC12 cell lysate at 25 µg
Predicted band size: 27 kDa
Observed band size: 21 kDa