Anti-Smac/Diablo antibody [SMAC 17 1-87]

• IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Smac/Diablo antibody [SMAC 17 1-87] (AB111893)

Immunohistochemistry (paraffin-treated sections) analysis of human colon tissue labelling SMAC/Diablo with ab111893 at 1/100 dilution. Tissues were counterstained with hematoxylin and dehydrated with xylene and ethanol. Negative control (left) has no primary antibody. Heat mediated antigen retrieval performed with citrate buffer pH 6 before commencing with IHC staining protocol.

• ICC/IF

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Immunocytochemistry/ Immunofluorescence - Anti-Smac/Diablo antibody [SMAC 17 1-87] (AB111893)

ab111893 labelling Smac / Diablo (green) in the cytoplasm of MCF-7 cells by Immunocytochemistry/Immunofluorescence. Formalin-fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 5-10 minutes and blocked with 3% BSA-PBS for 30 minutes at room temperature. Cells were incubated with the primary antibody (1 : 100 in 3% BSA-PBS) overnight at 4 °C. A DyLight-conjugated anti-mouse IgG was used as the secondary antibody. Red (phalloidiin) - F-actin, Blue - nuclei. Images were taken at a magnification of 60x.

• ICC/IF

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Immunocytochemistry/ Immunofluorescence - Anti-Smac/Diablo antibody [SMAC 17 1-87] (AB111893)

ab111893 labelling Smac / Diablo (green) in the cytoplasm of A431 cells by Immunocytochemistry/Immunofluorescence. Formalin-fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 5-10 minutes and blocked with 3% BSA-PBS for 30 minutes at room temperature. Cells were incubated with the primary antibody (1 : 100 in 3% BSA-PBS) overnight at 4 °C. A DyLight-conjugated anti-mouse IgG was used as the secondary antibody. Red (phalloidiin) - F-actin, Blue - nuclei. Images were taken at a magnification of 60x.

• IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Smac/Diablo antibody [SMAC 17 1-87] (AB111893)

Immunohistochemistry (paraffin-treated sections) analysis of human testis tissue labelling SMAC/Diablo with ab111893 at 1/100 dilution. Tissues were counterstained with hematoxylin and dehydrated with xylene and ethanol. Negative control (left) has no primary antibody. Heat mediated antigen retrieval performed with citrate buffer pH 6 before commencing with IHC staining protocol.

• IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Smac/Diablo antibody [SMAC 17 1-87] (AB111893)

Immunohistochemistry (paraffin-treated sections) analysis of mouse testis tissue labelling SMAC/Diablo with ab111893 at 1/100 dilution. Tissues were counterstained with hematoxylin and dehydrated with xylene and ethanol. Negative control (left) has no primary antibody. Heat mediated antigen retrieval performed with citrate buffer pH 6 before commencing with IHC staining protocol.

• ICC/IF

Supplier Data

Immunocytochemistry/ Immunofluorescence - Anti-Smac/Diablo antibody [SMAC 17 1-87] (AB111893)

ab111893 labelling Smac / Diablo (green) in the cytoplasm of PC12 cells by Immunocytochemistry/Immunofluorescence. Formalin-fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 5-10 minutes and blocked with 3% BSA-PBS for 30 minutes at room temperature. Cells were incubated with the primary antibody (1 : 100 in 3% BSA-PBS) overnight at 4 °C. A DyLight-conjugated anti-mouse IgG was used as the secondary antibody. Red (phalloidiin) - F-actin, Blue - nuclei. Images were taken at a magnification of 60x.

• WB

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Western blot - Anti-Smac/Diablo antibody [SMAC 17 1-87] (AB111893)

All lanes:

Western blot - Anti-Smac/Diablo antibody [SMAC 17 1-87] (ab111893) at 1/1000 dilution

Lane 1:

293 cell lysate at 25 µg

Lane 2:

HeLa cell lysate at 25 µg

Lane 3:

PC12 cell lysate at 25 µg

Predicted band size: 27 kDa

Observed band size: 21 kDa

false

• WB

Supplier Data

Western blot - Anti-Smac/Diablo antibody [SMAC 17 1-87] (AB111893)

All lanes:

Western blot - Anti-Smac/Diablo antibody [SMAC 17 1-87] (ab111893) at 1 µg/mL

Lane 1:

HeLa cell lysate at 30 µg

Lane 2:

Jurkat cell lysate at 30 µg

Lane 3:

MCF-7 cell lysate at 30 µg

Lane 4:

Hep G2 cell lysate at 30 µg

Lane 5:

NIH/3T3 cell lysate at 30 µg

Lane 6:

PC-12 cell lysate at 30 µg

Secondary

All lanes:

Goat anti-Mouse IgG (H+L) Superclonal™ Secondary Antibody, HRP conjugate at 1/4000 dilution

Predicted band size: 27 kDa

false