Rabbit Polyclonal SMAD1 antibody. Suitable for WB and reacts with Human samples. Cited in 6 publications. Immunogen corresponding to Synthetic Peptide within Human SMAD1 aa 1-50.
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- International journal of molecular sciences 23:2022SEC23B Loss-of-Function Suppresses Hepcidin Expression by Impairing Glycosylation Pathway in Human Hepatic Cells.Applications:Unspecified applicationReactive species:Unspecified reactive speciesBarbara Eleni Rosato,Roberta Marra,Vanessa D'Onofrio,Federica Del Giudice,Simone Della Monica,Achille Iolascon,Immacolata Andolfo,Roberta RussoPubMed 35163229
- Regenerative therapy 14:245-2512020Msx2 plays an important role in BMP6-induced osteogenic differentiation of two mesenchymal cell lines: C3H10T1/2 and C2C12.Applications:Unspecified applicationReactive species:Unspecified reactive speciesChuan Cai et. al.PubMed 32455154
- American journal of hematology 94:1227-12352019The BMP-SMAD pathway mediates the impaired hepatic iron metabolism associated with the ERFE-A260S variant.Applications:Unspecified applicationReactive species:Unspecified reactive speciesImmacolata Andolfo,Barbara Eleni Rosato,Roberta Marra,Gianluca De Rosa,Francesco Manna,Antonella Gambale,Achille Iolascon,Roberta RussoPubMed 31400017
- Journal of hepato-biliary-pancreatic sciences 26:219-2262019Propofol inhibits pancreatic cancer progress under hypoxia via ADAM8.Applications:Unspecified applicationReactive species:Unspecified reactive speciesYutong Gao et. al.PubMed 30945470
- Molecular medicine reports 18:2561-25702018Aucubin suppresses Titanium particles‑mediated apoptosis of MC3T3‑E1 cells and facilitates osteogenesis by affecting the BMP2/Smads/RunX2 signaling pathway.Applications:Unspecified applicationReactive species:Unspecified reactive speciesZiguan Zhu et. al.PubMed 30015916
- Journal of translational medicine 15:342017Bone Morphogenetic Proteins and myostatin pathways: key mediator of human sarcopenia.Applications:Unspecified applicationReactive species:Unspecified reactive speciesManuel Scimeca et. al.PubMed 28202082
Key facts
- Isotype
- IgG
- Host species
- Rabbit
- Storage buffer
pH: 7.4
Preservative: 0.05% Sodium azide
Constituents: PBS, 0.05% BSA- Form
- Liquid
- Clonality
- Polyclonal
Immunogen
- Synthetic Peptide within Human SMAD1 aa 1-50. The exact immunogen used to generate this antibody is proprietary information. Database link Q15797
Reactivity data
Select an application| WB | |
|---|---|
| Human | Tested |
WB
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info 0.50000-2.00000 µg/mL | Notes - |
Target data
Function
Transcriptional modulator that plays a role in various cellular processes, including embryonic development, cell differentiation, and tissue homeostasis (PubMed:9335504). Upon BMP ligand binding to their receptors at the cell surface, is phosphorylated by activated type I BMP receptors (BMPRIs) and associates with SMAD4 to form a heteromeric complex which translocates into the nucleus acting as transcription factor (PubMed:33667543). In turn, the hetero-trimeric complex recognizes cis-regulatory elements containing Smad Binding Elements (SBEs) to modulate the outcome of the signaling network (PubMed:33667543). SMAD1/OAZ1/PSMB4 complex mediates the degradation of the CREBBP/EP300 repressor SNIP1. Positively regulates BMP4-induced expression of odontogenic development regulator MSX1 following IPO7-mediated nuclear import (By similarity).
Additional Targets
SMAD9, SMAD5
Alternative names
BSP1, MADH1, MADR1, SMAD1, Mothers against decapentaplegic homolog 1, MAD homolog 1, Mothers against DPP homolog 1, JV4-1, Mad-related protein 1, SMAD family member 1, Transforming growth factor-beta-signaling protein 1, SMAD 1, Smad1, hSMAD1, BSP-1
Recommended products
Rabbit Polyclonal SMAD1 antibody. Suitable for WB and reacts with Human samples. Cited in 6 publications. Immunogen corresponding to Synthetic Peptide within Human SMAD1 aa 1-50.
Key facts
- Isotype
- IgG
- Host species
- Rabbit
- Storage buffer
pH: 7.4
Preservative: 0.05% Sodium azide
Constituents: PBS, 0.05% BSA- Form
- Liquid
- Clonality
- Polyclonal
- Immunogen
- Synthetic Peptide within Human SMAD1 aa 1-50. The exact immunogen used to generate this antibody is proprietary information. Database link Q15797
- Purification technique
- Affinity purification Protein G
- Specificity
ab13723 may cross-react with SMAD2 or SMAD3. The actual SMADs detected with this antibody may vary according to cell or tissue-type specific expression
- Concentration
Storage
- Shipped at conditions
- Blue Ice
- Appropriate short-term storage duration
- 1-2 weeks
- Appropriate short-term storage conditions
- +4°C
- Appropriate long-term storage conditions
- -20°C
- Aliquoting information
- Upon delivery aliquot
- Storage information
- Avoid freeze / thaw cycle
Notes
Abcam is leading the way to address reproducibility in scientific research with our highly validated recombinant monoclonal and recombinant multiclonal antibodies. Search & select one of Abcam's thousands of recombinant alternatives to eliminate batch-variability and unnecessary animal use.
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Supplementary info
- This supplementary information is collated from multiple sources and compiled automatically.
- Activity summary
SMAD 1/5/8/9 also known by the genes SMAD1 SMAD5 SMAD8 and SMAD9 are integral proteins in the transforming growth factor-beta (TGF-β) signaling pathway. These proteins function as transcriptional modulators once activated. The molecular weight of SMAD1 SMAD5 and SMAD8 ranges from approximately 60 to 65 kDa. These proteins are expressed in various tissues and are especially active in bone cartilage and the vascular system. They act by transmitting extracellular signals from TGF-β superfamily ligands to the nucleus leading to gene expression changes.
- Biological function summary
SMAD1/5/8/9 proteins play essential roles in processes like cellular proliferation differentiation and apoptosis. These proteins typically form complexes with other SMAD proteins such as SMAD4 upon activation by type I receptor kinases. The formation of these complexes is important for their translocation to the nucleus where they regulate target gene expression. This regulation is key in controlling pivotal developmental and homeostatic processes across different cell types.
- Pathways
SMAD1/5/8/9 proteins are central components of the bone morphogenetic protein (BMP) signaling pathway. They mediate the signal transduction from BMPs which are important for bone development and embryogenesis. These proteins interact with other signaling molecules such as BMP receptors and various co-SMADs like SMAD4 to relay and modulate signals effectively. Through these interactions SMAD1/5/8/9 function within a well-defined network ensuring precise cellular responses to BMP stimuli.
- Associated diseases and disorders
SMAD1/5/8/9 proteins show significant involvement in conditions like cancer and pulmonary arterial hypertension (PAH). Aberrant BMP signaling through dysfunctions in these SMAD proteins has been linked to tumorigenesis where abnormal cell proliferation is a hallmark. Additionally alterations in SMAD1/5/8/9 activity are associated with PAH a condition characterized by high blood pressure in the pulmonary arteries. In these diseases SMAD4 often acts as a partner in complex formation with SMAD1/5/8/9 underlining its importance in maintaining normal signaling processes.
Product promise
Tested
We have tested this species and application combination and it works. It is covered by our product promise.
Expected
We have not tested this specific species and application combination in-house, but expect it will work. It is covered by our product promise.
Predicted
This species and application combination has not been tested, but we predict it will work based on strong homology. However, this combination is not covered by our product promise.
Not recommended
We do not recommend this combination. It is not covered by our product promise.
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1 product image
Western blot - Anti-SMAD 1/5/8/9 antibody (ab13723)
Western blot analysis of SMAD1/5/8/9 in 15 ugs of A549 cell lysate using ab13723 at 0.5 ug/ml. Western blot analysis of SMAD1/5/8/9 in 15 ugs of A549 cell lysate using ab13723 at 0.5 ug/ml.
All lanes: Western blot - Anti-SMAD 1/5/8/9 antibody (ab13723)
Predicted band size: 52 kDa
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