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AB223072

Anti-SMAD 1 + SMAD9 antibody [EPR2290(2)] - BSA and Azide free

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(1 Publication)

Rabbit Recombinant Monoclonal SMAD1 antibody. Carrier free. Suitable for WB and reacts with Human samples. Cited in 1 publication.

View Alternative Names

BSP1, MADH1, MADR1, SMAD1, Mothers against decapentaplegic homolog 1, MAD homolog 1, Mothers against DPP homolog 1, JV4-1, Mad-related protein 1, SMAD family member 1, Transforming growth factor-beta-signaling protein 1, SMAD 1, Smad1, hSMAD1, BSP-1

1 Images
Western blot - Anti-SMAD 1 + SMAD9 antibody [EPR2290(2)] - BSA and Azide free (AB223072)
  • WB

Unknown

Western blot - Anti-SMAD 1 + SMAD9 antibody [EPR2290(2)] - BSA and Azide free (AB223072)

All lanes:

Western blot - Anti-SMAD 1 + SMAD9 antibody [EPR2290(2)] (<a href='/en-us/products/primary-antibodies/smad-1-smad9-antibody-epr22902-ab108965'>ab108965</a>) at 1/1000 dilution

Lane 1:

293T lysate at 10 µg

Lane 2:

A673 lysate at 10 µg

Lane 3:

Human heart lysate at 10 µg

Lane 4:

HeLa lysate at 10 µg

Secondary

All lanes:

HRP labelled goat anti-rabbit at 1/2000 dilution

Predicted band size: 52 kDa

Observed band size: 58 kDa

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Key facts

Host species

Rabbit

Clonality

Monoclonal

Clone number

EPR2290(2)

Isotype

IgG

Carrier free

Yes

Reacts with

Human

Applications

WB

applications

Immunogen

The exact immunogen used to generate this antibody is proprietary information.

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "ICCIF" : {"fullname" : "Immunocytochemistry/ Immunofluorescence", "shortname":"ICC/IF"}, "IP" : {"fullname" : "Immunoprecipitation", "shortname":"IP"}, "FlowCyt" : {"fullname" : "Flow Cytometry", "shortname":"Flow Cyt"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "ICCIF-species-checked": "notRecommended", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "<p></p>", "IP-species-checked": "notRecommended", "IP-species-dilution-info": "", "IP-species-notes": "<p></p>", "FlowCyt-species-checked": "notRecommended", "FlowCyt-species-dilution-info": "", "FlowCyt-species-notes": "<p></p>", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "", "WB-species-notes": "<p></p>", "IHCP-species-checked": "notRecommended", "IHCP-species-dilution-info": "", "IHCP-species-notes": "<p></p>" }, "Mouse": { "ICCIF-species-checked": "notRecommended", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "", "IP-species-checked": "notRecommended", "IP-species-dilution-info": "", "IP-species-notes": "", "FlowCyt-species-checked": "notRecommended", "FlowCyt-species-dilution-info": "", "FlowCyt-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "", "IHCP-species-checked": "notRecommended", "IHCP-species-dilution-info": "", "IHCP-species-notes": "" }, "Rat": { "ICCIF-species-checked": "notRecommended", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "", "IP-species-checked": "notRecommended", "IP-species-dilution-info": "", "IP-species-notes": "", "FlowCyt-species-checked": "notRecommended", "FlowCyt-species-dilution-info": "", "FlowCyt-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "", "IHCP-species-checked": "notRecommended", "IHCP-species-dilution-info": "", "IHCP-species-notes": "" } } }
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Product details

ab223072 is the carrier-free version of ab108965.

Patented technology
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:

  • - High batch-to-batch consistency and reproducibility
  • - Improved sensitivity and specificity
  • - Long-term security of supply
  • - Animal-free batch production

For more information, read more on recombinant antibodies.

Conjugation ready
Our carrier-free antibodies are typically supplied in a PBS-only formulation, purified and free of BSA, sodium azide and glycerol. This conjugation-ready format is designed for use with fluorochromes, metal isotopes, oligonucleotides, and enzymes, which makes them ideal for antibody labelling, functional and cell-based assays, flow-based assays (e.g. mass cytometry) and Multiplex Imaging applications.

Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with 1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.

Compatibility
This product is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm, without the need for antibody preparation. Maxpar® is a trademark of Fluidigm Canada Inc.

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Properties and storage information

Form
Liquid
Purification technique
Affinity purification Protein A
Storage buffer
pH: 7.2 - 7.4 Constituents: PBS
Shipped at conditions
Blue Ice
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
+4°C
Storage information
Do Not Freeze
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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

SMAD1 and SMAD9 also known as Mad1 and MadH1 are members of the SMAD family of proteins. These proteins are essential components of the BMP (Bone Morphogenetic Protein) signaling pathway. SMAD1 has a molecular weight of approximately 52 kDa while SMAD9 is slightly smaller. Both proteins are expressed in various tissues throughout the body including bone heart and brain where they contribute to cellular processes by transducing signals from the cell membrane to the nucleus.
Biological function summary

SMAD1 and SMAD9 mediate transcriptional responses to BMP signals influencing gene expression that governs cell growth differentiation and embryonic development. They function as part of a complex typically forming heterodimers with SMAD4. Upon BMP receptor activation SMAD1 and SMAD9 become phosphorylated which allows them to interact with SMAD4 and translocate to the nucleus to regulate target gene expression.

Pathways

SMAD1 and SMAD9 are key in the BMP signaling pathway and interact closely with SMAD4. The BMP pathway plays a critical role in developmental processes such as osteogenesis and chondrogenesis. SMAD1 and SMAD9 are also involved with the TGF-beta signaling pathway showing interaction with proteins like SMAD2 and SMAD3 through cross-talk which adds complexity to the regulatory circuitry for cell fate decisions.

SMAD1 and SMAD9 are associated with skeletal disorders and cancer. Mutations in the BMP pathway involving SMAD1 and SMAD9 can contribute to bone-related diseases such as fibrodysplasia ossificans progressiva. Their aberrant signaling is also linked to cancer development with disruptions in BMP signaling pathways potentially leading to tumor progression. These proteins are connected to other molecules like Noggin and Chordin which are involved in modulating BMP activity in these disorders.
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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:
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Target data

Transcriptional modulator that plays a role in various cellular processes, including embryonic development, cell differentiation, and tissue homeostasis (PubMed : 9335504). Upon BMP ligand binding to their receptors at the cell surface, is phosphorylated by activated type I BMP receptors (BMPRIs) and associates with SMAD4 to form a heteromeric complex which translocates into the nucleus acting as transcription factor (PubMed : 33667543). In turn, the hetero-trimeric complex recognizes cis-regulatory elements containing Smad Binding Elements (SBEs) to modulate the outcome of the signaling network (PubMed : 33667543). SMAD1/OAZ1/PSMB4 complex mediates the degradation of the CREBBP/EP300 repressor SNIP1. Positively regulates BMP4-induced expression of odontogenic development regulator MSX1 following IPO7-mediated nuclear import (By similarity).
See full target information SMAD1

Additional targets

SMAD9
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Publications (1)

Recent publications for all applications. Explore the full list and refine your search

Calcified tissue international 109:445-454 PubMed33884476

2021

Molecular Cloning of Mouse Homologue of Enamel Protein C4orf26 and Its Phosphorylation by FAM20C.

Applications

Unspecified application

Species

Unspecified reactive species

Nattanan Govitvattana,Masaru Kaku,Yoshio Ohyama,Haytham Jaha,I-Ping Lin,Hanna Mochida,Prasit Pavasant,Yoshiyuki Mochida
View all publications
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Product promise

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For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

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