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AB51451

Anti-SMAD1/3/5 (phospho S423 + S425) antibody

4

(15 Reviews)

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(60 Publications)

Rabbit Polyclonal SMAD3 phospho S423 + S425 antibody. Suitable for WB, IHC-P and reacts with Mouse, Human samples. Cited in 60 publications. Immunogen corresponding to Synthetic Peptide within Human SMAD3 phospho S423 + S425 aa 400 to C-terminus.

View Alternative Names

MADH3, SMAD3, Mothers against decapentaplegic homolog 3, MAD homolog 3, Mad3, Mothers against DPP homolog 3, hMAD-3, JV15-2, SMAD family member 3, SMAD 3, Smad3, hSMAD3

3 Images
Western blot - Anti-SMAD1/3/5 (phospho S423 + S425) antibody (AB51451)
  • WB

Supplier Data

Western blot - Anti-SMAD1/3/5 (phospho S423 + S425) antibody (AB51451)

All lanes:

Western blot - Anti-SMAD1/3/5 (phospho S423 + S425) antibody (ab51451) at 1 µg/mL

Lane 1:

HEK293 lysate at 10 µg

Lane 2:

HeLa Lysate at 10 µg

Lane 3:

MCF-7 Lysate at 10 µg

Lane 4:

Jurkat Lysate at 10 µg

Lane 5:

A431 Lysate at 10 µg

Lane 6:

A549 Lysate at 10 µg

Lane 7:

LNCap Lysate at 10 µg

Lane 8:

MOLT-4 Lysate at 10 µg

Lane 9:

Ramos Lysate at 10 µg

Lane 10:

Raji Lysate at 10 µg

Lane 11:

A-172 Lysate at 10 µg

Lane 12:

NIH/3T3 Lysate at 10 µg

Secondary

Lanes 1, 2, 4, 5, 6, 7, 8, 9, 10, 11 and 12:

Peroxidase rabbit secondary antibody at 1/30000 dilution

Lane 3:

Peroxidase rabbit secondary antibody

Predicted band size: 48 kDa

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Western blot - Anti-SMAD1/3/5 (phospho S423 + S425) antibody (AB51451)
  • WB

Unknown

Western blot - Anti-SMAD1/3/5 (phospho S423 + S425) antibody (AB51451)

Image courtesy of Ying Zhang, NIH, CCR, Bethesda, MD.

All lanes:

Western blot - Anti-SMAD1/3/5 (phospho S423 + S425) antibody (ab51451) at 1/2000 dilution

Lane 1:

MEF lysate (control)

Lane 2:

MEF lysate, after stimulation with 2 ng/ml of TGF-ß for 1 hour

Lane 3:

MEF<sup>-/-Smad3</sup> lysate (control)

Lane 4:

MEF<sup>-/-Smad3</sup> lysate, after stimulation with 2 ng/ml of TGF-ß for one hour

Lane 5:

Hep3B lysate (control)

Lane 6:

Hep3B lysate, after stimulation with 2 ng/ml of TGF-ß for one hour

Lane 7:

HEK 293 lysate (control)

Predicted band size: 48 kDa

false

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SMAD1/3/5 (phospho S423 + S425) antibody (AB51451)
  • IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SMAD1/3/5 (phospho S423 + S425) antibody (AB51451)

ab51451 at 2.5 μg/ml staining epidermal keratinocytes at 40X. This image shows strong nuclear staining with localization of the antibody as the precipated red siganl and a hematoxylin purple nuclear counterstain.

Key facts

Host species

Rabbit

Clonality

Polyclonal

Isotype

IgG

Carrier free

No

Reacts with

Mouse, Human

Applications

WB, IHC-P

applications

Immunogen

Synthetic Peptide within Human SMAD3 phospho S423 + S425 aa 400 to C-terminus. The exact immunogen used to generate this antibody is proprietary information.

P84022

Specificity

ab51451 is specific for the phosphorylated form of the protein although minimal reactivity with nonphosphorylated human Smad3 can be observed by ELISA and WB. ab51451 also reacts with phosphorylated Smad1 and Smad5 but negligible reactivity is seen against other phosphorylated Smad family members.

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/2000 - 1/20000", "WB-species-notes": "<p>Stimulation with 2 ng/ml TGF-beta for 1 hour is suggested.</p>", "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "1/500", "IHCP-species-notes": "<p></p>" }, "Mouse": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/2000 - 1/20000", "WB-species-notes": "<p>Stimulation with 2 ng/ml TGF-beta for 1 hour is suggested.</p>", "IHCP-species-checked": "guaranteed", "IHCP-species-dilution-info": "", "IHCP-species-notes": "" }, "Rat": { "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "", "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "" }, "Cow": { "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "", "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "" }, "Pig": { "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "", "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "" }, "Xenopus laevis": { "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "", "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "" }, "Zebrafish": { "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "", "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "" } } }
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Properties and storage information

Form
Liquid
Purification technique
Affinity purification Immunogen
Purification notes
ab51451 was affinity purified from monospecific antiserum by immunoaffinity purification. Antiserum was first purified against the phosphorylated form of the immunizing peptide. The resultant affinity purified antibody was then cross adsorbed against the non-phosphorylated form of the immunizing peptide.
Storage buffer
pH: 7.2 Preservative: 0.01% Sodium azide Constituents: 0.88% Sodium chloride, 0.424% Potassium phosphate solution
Shipped at conditions
Blue Ice
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
-20°C
Aliquoting information
Upon delivery aliquot
Storage information
Avoid freeze / thaw cycle
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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

SMAD1 SMAD3 and SMAD5 are intracellular proteins. They act as signal transducers in the transforming growth factor-beta (TGF-beta) signaling pathway. These proteins belong to the SMAD family and are sometimes referred to as mothers against decapentaplegic homolog or MADs. SMAD1 has a mass of about 52 kDa SMAD3 is around 48 kDa and SMAD5 is about 50 kDa. All three of them are expressed in a variety of tissues including muscle heart and brain. SMAD proteins undergo phosphorylation by serine/threonine kinase receptors which facilitates their role in signal transduction.
Biological function summary

SMAD1 SMAD3 and SMAD5 serve as transcription factors activated by external signaling molecules. Upon activation they form complexes with SMAD4 enabling them to translocate to the nucleus where they regulate gene expression. They influence cell proliferation differentiation and apoptosis playing a role in embryonic development and tissue homeostasis. They contribute to the regulation of genes involved in cell cycle control immune response and extracellular matrix production.

Pathways

The SMAD proteins are central to the TGF-beta signaling pathway and the bone morphogenetic protein (BMP) pathway. TGF-beta regulates numerous cellular processes using SMAD3 to propagate signals leading to fibronectin and collagen production. Conversely BMP signals are relayed via SMAD1 and SMAD5 which promote bone and cartilage development. These pathways are interlinked with other signaling molecules like TGF-beta receptors and BMP receptors governing various biological processes across cellular contexts.

SMAD1 SMAD3 and SMAD5 are linked to conditions such as cancer and fibrosis. Dysregulation of the TGF-beta pathway often involving SMAD3 contributes to tumor progression immune evasion and metastasis in cancers like breast and lung cancer. Mutations and overactivity in these pathways can also cause fibrotic diseases with SMAD3 being associated with diseases like systemic sclerosis. The interactions between SMAD proteins and TGF-beta receptors or SMAD4 in these diseases highlight their regulatory influence in pathological states.
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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:
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Target data

SMAD3 is a receptor-regulated SMAD that functions as an intracellular signal transducer and transcriptional modulator, activated by TGF-beta and activin type 1 receptor kinases. It binds to the TRE element in promoters of numerous genes regulated by TGF-beta, and upon forming a complex with SMAD4, activates transcription. Additionally, SMAD3 can form a complex with SMAD4, JUN, and FOS at the AP-1/SMAD site to regulate TGF-beta-mediated transcription. SMAD3 may inhibit wound healing by modulating the growth and migration of primary keratinocytes and altering TGF-beta-mediated monocyte chemotaxis, with this effect being potentially hormone-sensitive. Furthermore, SMAD3 is involved in regulating chondrogenesis and osteogenesis and may inhibit early bone fracture healing. It also positively regulates PDPK1 kinase activity by promoting its dissociation from the 14-3-3 protein YWHAQ, which negatively regulates it. This supplementary information is collated from multiple sources and compiled automatically.
See full target information SMAD3 phospho S423 + S425

Additional targets

SMAD1 phospho S463 + S465,SMAD5 phospho S463 + S465,SMAD5 phospho S463 + S465,SMAD1 phospho S463 + S465
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Publications (60)

Recent publications for all applications. Explore the full list and refine your search

Molecular biology of the cell 35:ar35 PubMed38170570

2024

Tonic ErbB signaling underlies TGFβ-induced activation of ERK and is required for lens cell epithelial to myofibroblast transition.

Applications

Unspecified application

Species

Unspecified reactive species

Judy K VanSlyke,Bruce A Boswell,Linda S Musil

Investigative ophthalmology & visual science 64:6 PubMed37418274

2023

ErbBs in Lens Cell Fibrosis and Secondary Cataract.

Applications

Unspecified application

Species

Unspecified reactive species

Judy K VanSlyke,Bruce A Boswell,Linda S Musil

Matrix biology : journal of the International Society for Matrix Biology 121:41-55 PubMed37217119

2023

TGFβ-2 haploinsufficiency causes early death in mice with Marfan syndrome.

Applications

Unspecified application

Species

Unspecified reactive species

Nalani Sachan,Colin K L Phoon,Lior Zilberberg,Matthias C Kugler,Taylor Ene,Shana B Mintz,Sae-Il Murtada,Dar Weiss,Glenn I Fishman,Jay D Humphrey,Daniel B Rifkin

eLife 11: PubMed36314781

2022

Sex-specific role of myostatin signaling in neonatal muscle growth, denervation atrophy, and neuromuscular contractures.

Applications

Unspecified application

Species

Unspecified reactive species

Marianne E Emmert,Parul Aggarwal,Kritton Shay-Winkler,Se-Jin Lee,Qingnian Goh,Roger Cornwall

Arteriosclerosis, thrombosis, and vascular biology 42:1244-1253 PubMed36073351

2022

The Nonproteolytic Intracellular Domain of Membrane-Type 1 Matrix Metalloproteinase Coordinately Modulates Abdominal Aortic Aneurysm and Atherosclerosis in Mice-Brief Report.

Applications

Unspecified application

Species

Unspecified reactive species

Michele Silvestro,Cristobal F Rivera,Dornazsadat Alebrahim,John Vlahos,Muhammad Yogi Pratama,Cuijie Lu,Claudia Tang,Zander Harpel,Rayan Sleiman Tellaoui,Ariadne L Zias,Delphina J Maldonado,Devon Byrd,Mukundan Attur,Paolo Mignatti,Bhama Ramkhelawon

Clinical and translational medicine 12:e758 PubMed35652284

2022

TGF-β1 mediates pathologic changes of secondary lymphedema by promoting fibrosis and inflammation.

Applications

Unspecified application

Species

Unspecified reactive species

Jung Eun Baik,Hyeung Ju Park,Raghu P Kataru,Ira L Savetsky,Catherine L Ly,Jinyeon Shin,Elizabeth M Encarnacion,Michele R Cavali,Mark G Klang,Elyn Riedel,Michelle Coriddi,Joseph H Dayan,Babak J Mehrara

Stem cells international 2022:1560943 PubMed35530413

2022

Palovarotene Can Attenuate Heterotopic Ossification Induced by Tendon Stem Cells by Downregulating the Synergistic Effects of Smad and NF-B Signaling Pathway following Stimulation of the Inflammatory Microenvironment.

Applications

Unspecified application

Species

Unspecified reactive species

Junchao Huang,Jialiang Lin,Congbin Li,Bo Tang,Haijun Xiao

The Journal of biological chemistry 296:100213 PubMed33779563

2021

KDM2B is involved in the epigenetic regulation of TGF-β-induced epithelial-mesenchymal transition in lung and pancreatic cancer cell lines.

Applications

Unspecified application

Species

Unspecified reactive species

Sasithorn Wanna-Udom,Minoru Terashima,Kusuma Suphakhong,Akihiko Ishimura,Takahisa Takino,Takeshi Suzuki

Frontiers in endocrinology 10:667 PubMed31632347

2019

The Interaction Between Nodal, Hypoxia-Inducible Factor 1 Alpha, and Thrombospondin 1 Promotes Luteolysis in Equine Corpus Luteum.

Applications

Unspecified application

Species

Unspecified reactive species

Edyta Walewska,Karolina Wołodko,Dariusz Skarzynski,Graça Ferreira-Dias,António Galvão

JCI insight 5: PubMed30913038

2019

Single cell RNA sequencing identifies TGFβ as a key regenerative cue following LPS-induced lung injury.

Applications

Unspecified application

Species

Unspecified reactive species

Kent A Riemondy,Nicole L Jansing,Peng Jiang,Elizabeth F Redente,Austin E Gillen,Rui Fu,Alyssa J Miller,Jason R Spence,Anthony N Gerber,Jay R Hesselberth,Rachel L Zemans
View all publications
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