Anti-SMAD1/5/9 antibody

• WB

Unknown

Western blot - Anti-SMAD1/5/9 antibody (AB80255)

All lanes:

Western blot - Anti-SMAD1/5/9 antibody (ab80255) at 1/500 dilution

Lane 1:

HeLa cell extracts at 30 µg

Lane 2:

HeLa cell extracts at 30 µg with immunising peptide

Predicted band size: 52 kDa

Observed band size: 52 kDa

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• WB

CiteAb

Western blot - Anti-SMAD1/5/9 antibody (AB80255)

SMAD1/5/9 western blot using anti-SMAD1/5/9 antibody ab80255. Publication image and figure legend from Huang, K. C., Chuang, P. Y., et al., 2020, Int J Mol Sci, PubMed 32630668.

ab80255 was used in this publication in western blot. This may not be the same as the application(s) guaranteed by Abcam. For a full list of applications guaranteed by Abcam for ab80255 please see the product overview.

Smad1/5 signaling regulates 20 dynes/cm2 shear force-induced SCD-1 expression in human MG63 osteosarcoma cells. (A) Cells were kept as the controls (CL) or stimulated with 20 dynes/cm2 shear force for 1, 4, 8 and 24 h, and their Smad1/5 phosphorylation was analyzed by Western blot. (B,C) Cells were transfected with control-, Smad1-, or Smad5-specific siRNA (siCL, siSmad1, siSmad5) and then kept as the controls (CL) or stimulated with 20 dynes/cm2 shear force (SS) for 8 h. (B) The SCD-1 protein expression was analyzed by Western blot. (C) The Smad1 or Smad5 gene expressions were analyzed by real-time quantitative PCR. Results in (A,B) are representative of three independent experiments with similar results. Statistical data in (A–C) are mean ± SEM from three independent experiments. Protein phosphorylation and expression (A,B) and mRNA expression (C) levels were normalized to Smad1/5/β-actin and GAPDH, respectively. * p < 0.05 versus untreated control cells (CL), siCL-CL, or siCL. # p < 0.05 versus siCL-SS-treated cells.

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