AB53745

Anti-Smad1 antibody [913C1b]

Name: Anti-Smad1 antibody [913C1b] (ab53745) | Abcam
Brand: Abcam Limited
SKU: AB53745
Price: 545 USD
Availability: InStock
Rating: 4 (1 reviews)

(1 Review)

(4 Publications)

Mouse Monoclonal SMAD1 antibody. Suitable for Flow Cyt, WB and reacts with Human, Mouse, Rat samples. Cited in 4 publications. Immunogen corresponding to Recombinant Fragment Protein within Human SMAD1.

View Alternative Names

BSP1, MADH1, MADR1, SMAD1, SMAD family member 1, SMAD 1, hSMAD1, JV4-1, Mad-related protein 1, Mothers against decapentaplegic homolog 1, Transforming growth factor-beta-signaling protein 1, MAD homolog 1, Mothers against DPP homolog 1, BSP-1

4 Images

Flow Cytometry - Anti-Smad1 antibody [913C1b] (AB53745)

Flow Cyt

Unknown

Flow Cytometry - Anti-Smad1 antibody [913C1b] (AB53745)

Using 0.5μg Anti-SMAD1 antibody (ab53745) and 0.25μg Goat anti-Mouse secondary.

Unknown

Western blot - Anti-Smad1 antibody [913C1b] (AB53745)

All lanes:

Western blot - Anti-Smad1 antibody [913C1b] (ab53745) at 1/50 dilution

All lanes:

NIH3T3 whole cell lysate at 25 µg

Secondary

All lanes:

Mouse IgG at 1/2500 dilution

Predicted band size: 52 kDa

Observed band size: 62 kDa

false

Unknown

Western blot - Anti-Smad1 antibody [913C1b] (AB53745)

All lanes:

Western blot - Anti-Smad1 antibody [913C1b] (ab53745) at 1/50 dilution

All lanes:

HEK293 whole cell lysate at 25 µg

Secondary

All lanes:

Mouse IgG antibody at 1/2500 dilution

Predicted band size: 52 kDa

Observed band size: 60 kDa

false

Unknown

Western blot - Anti-Smad1 antibody [913C1b] (AB53745)

All lanes:

Western blot - Anti-Smad1 antibody [913C1b] (ab53745) at 1/50 dilution

All lanes:

F2408 whole cell lysate at 25 µg

Secondary

All lanes:

Mouse IgG at 1/2500 dilution

Predicted band size: 52 kDa

Observed band size: 62 kDa

false

Key facts

Host species

Mouse

Clonality

Monoclonal

Clone number

913C1b

Isotype

IgG1

Carrier free

Reacts with

Mouse, Rat, Human

Applications

Flow Cyt, WB

applications

Immunogen

Recombinant Fragment Protein within Human SMAD1. The exact immunogen used to generate this antibody is proprietary information.

Q15797

style

left-column

style

left-column

Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "FlowCyt" : {"fullname" : "Flow Cytometry", "shortname":"Flow Cyt"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "FlowCyt-species-checked": "testedAndGuaranteed", "FlowCyt-species-dilution-info": "", "FlowCyt-species-notes": "<a href='/en-us/products/primary-antibodies/mouse-igg1-kappa-monoclonal-15-6e10a7-isotype-control-ab170190'>ab170190</a> - Mouse monoclonal IgG1, is suitable for use as an isotype control with this antibody.", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/50", "WB-species-notes": "" }, "Mouse": { "FlowCyt-species-checked": "guaranteed", "FlowCyt-species-dilution-info": "", "FlowCyt-species-notes": "", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/50", "WB-species-notes": "" }, "Rat": { "FlowCyt-species-checked": "guaranteed", "FlowCyt-species-dilution-info": "", "FlowCyt-species-notes": "", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/50", "WB-species-notes": "" } } }

style

left-column

style

left-column

Properties and storage information

Form

Liquid

Purification technique

Affinity purification Protein G

Storage buffer

pH: 7.4 Preservative: 0.05% Sodium azide Constituents: 1% BSA, 0.812% Sodium chloride, 0.1312% Sodium phosphate, 0.1136% Disodium hydrogenorthophosphate, 0.03% Tripotassium orthophosphate, 0.0225% Potassium chloride, 0.0204% Potassium phosphate monobasic

Shipped at conditions

Blue Ice

Appropriate short-term storage duration

Up to 12 months

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

-20°C

style

left-column

Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

Smad1 also known as Mothers against decapentaplegic homolog 1 (MADH1) is a protein encoded by the SMAD1 gene in humans. It has a molecular mass of approximately 53 kDa and functions as a receptor-regulated Smad protein. Smad1 primarily transmits signals from the bone morphogenetic proteins (BMPs) a group of growth factors and cytokines. Expression of Smad1 occurs in various tissues including the lung heart and kidney. It plays an important role in mediating signaling pathways within different cellular environments.

Biological function summary

Smad1 participates in the transmission of BMP signals from the cell surface to the nucleus ensuring transcriptional regulation of target genes. In the presence of BMPs Smad1 forms complexes with Smad4 upon activation. These Smad complexes then translocate to the nucleus where they regulate gene expression. Smad1 influences cellular responses such as proliferation differentiation and apoptosis. In particular it plays a significant role in bone development and osteogenesis.

Pathways

Smad1 plays an important role within the BMP signaling pathway which is important for early development and tissue homeostasis. Within this pathway BMPs trigger the phosphorylation of Smad1 which then associates with Smad4 to propagate downstream signaling. Another associated pathway includes the TGF-beta signaling pathway where Smads like Smad2 and Smad3 show functional similarities and differences with Smad1. The interactions of Smad1 with related proteins like Smad4 highlight its significant contribution to cellular processes regulated by these pathways.

Smad1 associates with various pathological conditions. For instance aberrant Smad1 signaling has implications in cancer particularly in processes like tumor progression and metastasis. Disruptions in the BMP pathway involving Smad1 and Smad4 can influence the onset and development of disorders such as pulmonary hypertension where inappropriate cell growth and remodeling occur. Understanding Smad1's role in these contexts provides insights into potential therapeutic avenues for treatment and management of these conditions.

style

left-column

style

left-column

Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

style

left-column

Target data

Transcriptional modulator that plays a role in various cellular processes, including embryonic development, cell differentiation, and tissue homeostasis (PubMed : 9335504). Upon BMP ligand binding to their receptors at the cell surface, is phosphorylated by activated type I BMP receptors (BMPRIs) and associates with SMAD4 to form a heteromeric complex which translocates into the nucleus acting as transcription factor (PubMed : 33667543). In turn, the hetero-trimeric complex recognizes cis-regulatory elements containing Smad Binding Elements (SBEs) to modulate the outcome of the signaling network (PubMed : 33667543). SMAD1/OAZ1/PSMB4 complex mediates the degradation of the CREBBP/EP300 repressor SNIP1. Positively regulates BMP4-induced expression of odontogenic development regulator MSX1 following IPO7-mediated nuclear import (By similarity).

See full target information SMAD1

style

left-column

Publications (4)

Recent publications for all applications. Explore the full list and refine your search

Brazilian journal of medical and biological research = Revista brasileira de pesquisas medicas e biologicas 58:e14368 PubMed39907411

2025

N-glycosylation of ACTRIIB enhances protein stability leading to rapid cell proliferation and strong resistance to docetaxel in nasopharyngeal carcinoma.

Applications

Unspecified application

Species

Unspecified reactive species

Qin Qin,Junfeng Li,Yinjian Shao,Lan Liu,Zhibin Luo

BMC biology 20:210 PubMed36171573

2022

Atheroprone fluid shear stress-regulated ALK1-Endoglin-SMAD signaling originates from early endosomes.

Applications

Unspecified application

Species

Unspecified reactive species

Paul-Lennard Mendez,Leon Obendorf,Jerome Jatzlau,Wiktor Burdzinski,Maria Reichenbach,Vanasa Nageswaran,Arash Haghikia,Verena Stangl,Christian Hiepen,Petra Knaus

Journal of nanobiotechnology 19:226 PubMed34340698

2021

MiR-144-5p, an exosomal miRNA from bone marrow-derived macrophage in type 2 diabetes, impairs bone fracture healing via targeting Smad1.

Applications

Unspecified application

Species

Unspecified reactive species

Dong Zhang,Yifan Wu,Zonghuan Li,Hairen Chen,Siyuan Huang,Chao Jian,Aixi Yu

Oncogene 37:2515-2531 PubMed29449696

2018

Snail regulates BMP and TGFβ pathways to control the differentiation status of glioma-initiating cells.

Applications

Unspecified application

Species

Unspecified reactive species

Laia Caja,Kalliopi Tzavlaki,Mahsa S Dadras,E-Jean Tan,Gad Hatem,Naga P Maturi,Anita Morén,Lotta Wik,Yukihide Watanabe,Katia Savary,Masood Kamali-Moghaddan,Lene Uhrbom,Carl-Henrik Heldin,Aristidis Moustakas

View all publications

style

left-column

style

left-column

style

right-column

Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.

For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com

Anti-Smad1 antibody [913C1b]

Flow Cytometry - Anti-Smad1 antibody [913C1b] (AB53745)

Western blot - Anti-Smad1 antibody [913C1b] (AB53745)

Western blot - Anti-Smad1 antibody [913C1b] (AB53745)

Western blot - Anti-Smad1 antibody [913C1b] (AB53745)

Key facts

Host species

Clonality

Clone number

Isotype

Carrier free

Reacts with

Applications

Immunogen

Alternative Products

Primary Antibodies

Primary Antibodies

Primary Antibodies

Primary Antibodies

Primary Antibodies

Primary Antibodies

Primary Antibodies

Complementary Products

Assay Kits

Secondary Antibodies

Primary Antibodies

Primary Antibodies

Primary Antibodies

Reactivity data

Properties and storage information

Form

Purification technique

Storage buffer

Shipped at conditions

Appropriate short-term storage duration

Appropriate short-term storage conditions

Appropriate long-term storage conditions

Supplementary information

Biological function summary

Pathways

Product protocols

Target data

Publications (4)

Product promise