AB73211

Anti-Smad1 (phospho S187) antibody

Name: Anti-Smad1 (phospho S187) antibody (ab73211) | Abcam
Brand: Abcam Limited
SKU: AB73211
Rating: 4 (1 reviews)

(1 Review)

(13 Publications)

Rabbit Polyclonal SMAD1 phospho S187 antibody. Suitable for WB, IHC-P and reacts with Mouse, Human samples. Cited in 13 publications. Immunogen corresponding to Synthetic Peptide within Human SMAD1 pS187.

View Alternative Names

BSP1, MADH1, MADR1, SMAD1, SMAD family member 1, SMAD 1, hSMAD1, JV4-1, Mad-related protein 1, Mothers against decapentaplegic homolog 1, Transforming growth factor-beta-signaling protein 1, MAD homolog 1, Mothers against DPP homolog 1, BSP-1

2 Images

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Smad1 (phospho S187) antibody (AB73211)

IHC-P

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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Smad1 (phospho S187) antibody (AB73211)

Immunohistochemical analysis of Smad1 in paraffin-embedded human breast carcinoma tissue using ab73211 at 1/50 dilution in the presence (right panel) or absence (left panel) of immunising phosphopeptide.

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Western blot - Anti-Smad1 (phospho S187) antibody (AB73211)

All lanes:

Western blot - Anti-Smad1 (phospho S187) antibody (ab73211) at 1/500 dilution

Lane 1:

Mouse muscle cell extract at 30 µg

Lane 2:

Mouse muscle cell extract at 30 µg with immunising phosphopeptide

Predicted band size: 52 kDa

Observed band size: 60 kDa

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Key facts

Host species

Rabbit

Clonality

Polyclonal

Isotype

IgG

Carrier free

Reacts with

Mouse, Human

Applications

IHC-P, WB

applications

Immunogen

Synthetic Peptide within Human SMAD1 pS187. The exact immunogen used to generate this antibody is proprietary information.

Q15797

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "WB-species-checked": "guaranteed", "WB-species-dilution-info": "", "WB-species-notes": "", "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "", "IHCP-species-notes": "<p></p>" }, "Mouse": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/500 - 1/1000", "WB-species-notes": "<p></p>", "IHCP-species-checked": "guaranteed", "IHCP-species-dilution-info": "", "IHCP-species-notes": "" } } }

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Properties and storage information

Form

Liquid

Purification technique

Affinity purification Immunogen

Purification notes

ab73211 was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific phosphopeptide. The antibody against non-phosphopeptide was removed by chromatography using non-phosphopeptide corresponding to the phosphorylation site.

Storage buffer

pH: 7.4 Preservative: 0.02% Sodium azide Constituents: PBS, 50% Glycerol (glycerin, glycerine), 0.87% Sodium chloride

Shipped at conditions

Blue Ice

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

-20°C

Storage information

Stable for 12 months at -20°C

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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

Smad1 also known as Mothers against decapentaplegic homolog 1 (MADH1) is a protein encoded by the SMAD1 gene in humans. It has a molecular mass of approximately 53 kDa and functions as a receptor-regulated Smad protein. Smad1 primarily transmits signals from the bone morphogenetic proteins (BMPs) a group of growth factors and cytokines. Expression of Smad1 occurs in various tissues including the lung heart and kidney. It plays an important role in mediating signaling pathways within different cellular environments.

Biological function summary

Smad1 participates in the transmission of BMP signals from the cell surface to the nucleus ensuring transcriptional regulation of target genes. In the presence of BMPs Smad1 forms complexes with Smad4 upon activation. These Smad complexes then translocate to the nucleus where they regulate gene expression. Smad1 influences cellular responses such as proliferation differentiation and apoptosis. In particular it plays a significant role in bone development and osteogenesis.

Pathways

Smad1 plays an important role within the BMP signaling pathway which is important for early development and tissue homeostasis. Within this pathway BMPs trigger the phosphorylation of Smad1 which then associates with Smad4 to propagate downstream signaling. Another associated pathway includes the TGF-beta signaling pathway where Smads like Smad2 and Smad3 show functional similarities and differences with Smad1. The interactions of Smad1 with related proteins like Smad4 highlight its significant contribution to cellular processes regulated by these pathways.

Smad1 associates with various pathological conditions. For instance aberrant Smad1 signaling has implications in cancer particularly in processes like tumor progression and metastasis. Disruptions in the BMP pathway involving Smad1 and Smad4 can influence the onset and development of disorders such as pulmonary hypertension where inappropriate cell growth and remodeling occur. Understanding Smad1's role in these contexts provides insights into potential therapeutic avenues for treatment and management of these conditions.

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

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Target data

Transcriptional modulator that plays a role in various cellular processes, including embryonic development, cell differentiation, and tissue homeostasis (PubMed : 9335504). Upon BMP ligand binding to their receptors at the cell surface, is phosphorylated by activated type I BMP receptors (BMPRIs) and associates with SMAD4 to form a heteromeric complex which translocates into the nucleus acting as transcription factor (PubMed : 33667543). In turn, the hetero-trimeric complex recognizes cis-regulatory elements containing Smad Binding Elements (SBEs) to modulate the outcome of the signaling network (PubMed : 33667543). SMAD1/OAZ1/PSMB4 complex mediates the degradation of the CREBBP/EP300 repressor SNIP1. Positively regulates BMP4-induced expression of odontogenic development regulator MSX1 following IPO7-mediated nuclear import (By similarity).

See full target information SMAD1 pS187

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Publications (13)

Recent publications for all applications. Explore the full list and refine your search

Osteoarthritis and cartilage open 5:100399 PubMed37649532

2023

Depleting transforming growth factor beta receptor 2 signalling in the cartilage of -null mice attenuates spontaneous knee osteoarthritis.

Applications

Unspecified application

Species

Unspecified reactive species

Jennifer St Amant,Jana Michaud,Daniel Hinds,Madison Coyle,Ambra Pozzi,Andrea L Clark

Journal of advanced research 54:29-42 PubMed36736696

2023

LncRNA NIPA1-SO confers atherosclerotic protection by suppressing the transmembrane protein NIPA1.

Applications

Unspecified application

Species

Unspecified reactive species

Min Jiang,Yu Song,Mei-Xia Ren,Run-Chao He,Xian-Hui Dong,Xue-Heng Li,Zhi-Feng Lu,Shu Li,Jia Wu,Yan-Rou Bei,Fei Liu,Yan Long,Shao-Guo Wu,Xue-Hui Liu,Li-Mei Wu,Hong-Ling Yang,David G McVey,Xiao-Yan Dai,Shu Ye,Yan-Wei Hu

Aging 13:10275-10288 PubMed33819188

2021

CircRNA hsa_circ_0006215 promotes osteogenic differentiation of BMSCs and enhances osteogenesis-angiogenesis coupling by competitively binding to miR-942-5p and regulating RUNX2 and VEGF.

Applications

Unspecified application

Species

Unspecified reactive species

Houlin Ji,Xu Cui,Yang Yang,Xiaoxiao Zhou

Bone research 8:41 PubMed33298874

2020

A novel negative regulatory mechanism of Smurf2 in BMP/Smad signaling in bone.

Applications

Unspecified application

Species

Unspecified reactive species

Junichi Kushioka,Takashi Kaito,Rintaro Okada,Hiroyuki Ishiguro,Zeynep Bal,Joe Kodama,Ryota Chijimatsu,Melanie Pye,Masahiro Narimatsu,Jeffrey L Wrana,Yasumichi Inoue,Hiroko Ninomiya,Shin Yamamoto,Takashi Saitou,Hideki Yoshikawa,Takeshi Imamura

Pathogens (Basel, Switzerland) 9: PubMed32708289

2020

The Epstein-Barr Virus-Encoded EBNA1 Protein Activates the Bone Morphogenic Protein (BMP) Signalling Pathway to Promote Carcinoma Cell Migration.

Applications

Unspecified application

Species

Unspecified reactive species

Hannah E Bridgewater,Kathryn L Date,John D O'Neil,Chunfang Hu,John R Arrand,Christopher W Dawson,Lawrence S Young

Cell death & disease 11:486 PubMed32587236

2020

LncRNA ENST00000563492 promoting the osteogenesis-angiogenesis coupling process in bone mesenchymal stem cells (BMSCs) by functions as a ceRNA for miR-205-5p.

Applications

Unspecified application

Species

Unspecified reactive species

Zhengxiao Ouyang,Tingting Tan,Xianghong Zhang,Jia Wan,Yanling Zhou,Guangyao Jiang,Daishui Yang,Tang Liu

Molecular medicine reports 20:2167-2176 PubMed31322216

2019

Downregulation of miR‑98 contributes to hypoxic pulmonary hypertension by targeting ALK1.

Applications

Unspecified application

Species

Unspecified reactive species

Qingling Li,Xincan Zhou,Xianghui Zhou

Cellular and molecular life sciences : CMLS 76:4795-4809 PubMed31201465

2019

Adamts17 is involved in skeletogenesis through modulation of BMP-Smad1/5/8 pathway.

Applications

Unspecified application

Species

Unspecified reactive species

Takeshi Oichi,Yuki Taniguchi,Kazuhito Soma,Yasushi Oshima,Fumiko Yano,Yoshifumi Mori,Ryota Chijimatsu,Joo-Ri Kim-Kaneyama,Sakae Tanaka,Taku Saito

Frontiers in immunology 9:647 PubMed29651293

2018

Homeobox a5 Promotes White Adipose Tissue Browning Through Inhibition of the Tenascin C/Toll-Like Receptor 4/Nuclear Factor Kappa B Inflammatory Signaling in Mice.

Applications

Unspecified application

Species

Unspecified reactive species

Weina Cao,Hongtao Huang,Tianyu Xia,Chenlong Liu,Saeed Muhammad,Chao Sun

International journal of molecular medicine 41:2517-2526 PubMed29484386

2018

Mangiferin inhibits apoptosis and oxidative stress via BMP2/Smad-1 signaling in dexamethasone-induced MC3T3-E1 cells.

Applications

Unspecified application

Species

Unspecified reactive species

Ling-Zhi Ding,Xiao Teng,Zhao-Bo Zhang,Chang-Jun Zheng,Shi-Hong Chen

View all publications

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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.

For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

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Anti-Smad1 (phospho S187) antibody

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Smad1 (phospho S187) antibody (AB73211)

Western blot - Anti-Smad1 (phospho S187) antibody (AB73211)

Key facts

Host species

Clonality

Isotype

Carrier free

Reacts with

Applications

Immunogen

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Reactivity data

Properties and storage information

Form

Purification technique

Purification notes

Storage buffer

Shipped at conditions

Appropriate short-term storage conditions

Appropriate long-term storage conditions

Storage information

Supplementary information

Biological function summary

Pathways

Product protocols

Target data

Publications (13)

Product promise