AB214423

Anti-Smad1 (phospho S463 + S465) antibody [EPR20662-29]

20ul selling size
RabMAb
Recombinant
What is this?

Be the first to review this product! Submit a review

(5 Publications)

Rabbit Recombinant Monoclonal SMAD1 phospho S463 + S465 antibody. Suitable for IP, Dot, WB and reacts with Mouse, Human, Synthetic peptide samples. Cited in 5 publications.

View Alternative Names

BSP1, MADH1, MADR1, SMAD1, Mothers against decapentaplegic homolog 1, MAD homolog 1, Mothers against DPP homolog 1, JV4-1, Mad-related protein 1, SMAD family member 1, Transforming growth factor-beta-signaling protein 1, SMAD 1, Smad1, hSMAD1, BSP-1

4 Images

Lab

Western blot - Anti-Smad1 (phospho S463 + S465) antibody [EPR20662-29] (AB214423)

Blocking/Dilution : 5% NFDM/TBST.

All lanes:

Western blot - Anti-Smad1 (phospho S463 + S465) antibody [EPR20662-29] (ab214423) at 1/1000 dilution

Lane 1:

HeLa (human cervix adenocarcinoma epithelial cell) grown in serum-free media overnight, whole cell lysate at 20 µg

Lane 2:

HeLa grown in serum-free media overnight, then treated with 100ng/ml Calyculin A (<a href='/en-us/products/biochemicals/calyculin-a-protein-phosphatase-inhibitor-ab141784'>ab141784</a>) for 15 minutes, Calyculin A was removed, followed by treatment with 100ng/ml BMP2 for 30 minutes, whole cell lysate at 20 µg

Lane 3:

NIH/3T3 (mouse embryonic fibroblast) grown in serum-free media overnight, whole cell lysate at 20 µg

Lane 4:

NIH/3T3 grown in serum-free media overnight, then treated with 50ng/ml BMP2 for 30 minutes, whole cell lysate at 20 µg

Secondary

All lanes:

Western blot - Goat Anti-Rabbit IgG H&L (HRP) (<a href='/en-us/products/secondary-antibodies/goat-rabbit-igg-h-l-hrp-ab97051'>ab97051</a>) at 1/100000 dilution

Predicted band size: 52 kDa

Observed band size: 60 kDa

true

Exposure time: 10s

Supplier Data

Immunoprecipitation - Anti-Smad1 (phospho S463 + S465) antibody [EPR20662-29] (AB214423)

Smad1 (phospho S463/S465) was immunoprecipitated from 0.35 mg of NIH/3T3 (mouse embryo fibroblast cell line) whole cell lysate with ab214423 at 1/30 dilution. Western blot was performed from the immunoprecipitate using ab214423 at 1/500 dilution. VeriBlot for IP Detection Reagent (HRP) (ab131366), was used for detection at 1/1,000 dilution

Lane 1 : NIH/3T3 (mouse embryonic fibroblast) grown in serum-free media overnight, then treated with 50 ng/ml BMP2 for 30 minutes, whole cell lysate 10 μg (Input).
Lane 2 : ab214423 IP in NIH/3T3 grown in serum-free media overnight, then treated with 50 ng/ml BMP2 for 30 minutes, whole cell lysate.
Lane 3 : Rabbit monoclonal IgG (ab172730) instead of ab214423 in NIH/3T3 grown in serum-free media overnight, then treated with 50 ng/ml BMP2 for 30 minutes, whole cell lysate

Blocking and dilution buffer : 5% NFDM/TBST.

All lanes:

Immunoprecipitation - Anti-Smad1 (phospho S463 + S465) antibody [EPR20662-29] (ab214423)

Predicted band size: 52 kDa

Observed band size: 60 kDa

true

Exposure time: 30s

Supplier Data

Dot Blot - Anti-Smad1 (phospho S463 + S465) antibody [EPR20662-29] (AB214423)

Dot blot analysis of Smad1 (phospho S463/S465) peptide (Lane 1), Smad1 (phospho S463) peptide (Lane 2), Smad1 (phospho S465) peptide (Lane 3), Smad1 non-phospho peptide (Lane 4), Smad5 (phospho S463/S465) peptide (Lane 5), Smad5 (phospho S463) peptide (Lane 6), Smad5 (phospho S465) peptide (Lane 7) and Smad5 non-phospho peptide (Lane 8) using ab214423 at 1/1,000 dilution followed by Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated (ab97051) at 1/100,000 dilution.

Blocking and Diluting buffer and concentration : 5% NFDM /TBST.

Exposure time : 30 seconds.

CiteAb

Western blot - Anti-Smad1 (phospho S463 + S465) antibody [EPR20662-29] (AB214423)

Smad1 (phospho S463 + S465) western blot using anti-Smad1 (phospho S463 + S465) antibody [EPR20662-29] ab214423. Publication image and figure legend from Pei, D. D., Sun, J. L., et al., 2018, Adv Sci (Weinh), PubMed 30356983.

ab214423 was used in this publication in western blot. This may not be the same as the application(s) guaranteed by Abcam. For a full list of applications guaranteed by Abcam for ab214423 please see the product overview.

ALP activity and protein expression level of PINK1-silenced hDPSCs. A) Western blot results of selected mitophagy-related and mineralization-related proteins obtained from PINK1-silenced hDPSCs treated with or without CCCP. B) Results of ALP staining and ALP enzyme activity. C) Western blot results of Smad1/5/8 and phosphate Smad1 expression. For each target, group columns identified with red asterisks were significantly different from the control group (Lv-Control, p < 0.05).

false

Carrier free

Anti-Smad1 (phospho S463 + S465) antibody [EPR20662-29] - BSA and Azide free

Key facts

Host species

Rabbit

Clonality

Monoclonal

Clone number

EPR20662-29

Isotype

IgG

Carrier free

Reacts with

Mouse, Human

Applications

WB, IP, Dot

applications

Immunogen

The exact immunogen used to generate this antibody is proprietary information.

Specificity

Based on sequence homology this antibody also reacts with Smad5 (phospho S463/S465) and Smad9 (phospho S465/S467).

style

left-column

Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "IP" : {"fullname" : "Immunoprecipitation", "shortname":"IP"}, "Dot" : {"fullname" : "Dot Blot", "shortname":"Dot"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "IP-species-checked": "guaranteed", "IP-species-dilution-info": "", "IP-species-notes": "", "Dot-species-checked": "guaranteed", "Dot-species-dilution-info": "", "Dot-species-notes": "", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/1000", "WB-species-notes": "" }, "Mouse": { "IP-species-checked": "testedAndGuaranteed", "IP-species-dilution-info": "1/30", "IP-species-notes": "", "Dot-species-checked": "guaranteed", "Dot-species-dilution-info": "", "Dot-species-notes": "", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/1000", "WB-species-notes": "" }, "Synthetic peptide": { "IP-species-checked": "notRecommended", "IP-species-dilution-info": "", "IP-species-notes": "", "Dot-species-checked": "testedAndGuaranteed", "Dot-species-dilution-info": "1/1000", "Dot-species-notes": "", "WB-species-checked": "notRecommended", "WB-species-dilution-info": "", "WB-species-notes": "" } } }

style

left-column

Product details

Patented technology
Our RabMAb^® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb^® patents.

style

left-column

Properties and storage information

Form

Liquid

Purification technique

Affinity purification Protein A

Storage buffer

pH: 7.2 - 7.4 Preservative: 0.01% Sodium azide Constituents: PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA

Shipped at conditions

Blue Ice

Appropriate short-term storage duration

1-2 weeks

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

-20°C

Aliquoting information

Upon delivery aliquot

Storage information

Avoid freeze / thaw cycle

style

left-column

Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

Smad1 also known as Mothers against decapentaplegic homolog 1 (MADH1) is a protein encoded by the SMAD1 gene in humans. It has a molecular mass of approximately 53 kDa and functions as a receptor-regulated Smad protein. Smad1 primarily transmits signals from the bone morphogenetic proteins (BMPs) a group of growth factors and cytokines. Expression of Smad1 occurs in various tissues including the lung heart and kidney. It plays an important role in mediating signaling pathways within different cellular environments.

Biological function summary

Smad1 participates in the transmission of BMP signals from the cell surface to the nucleus ensuring transcriptional regulation of target genes. In the presence of BMPs Smad1 forms complexes with Smad4 upon activation. These Smad complexes then translocate to the nucleus where they regulate gene expression. Smad1 influences cellular responses such as proliferation differentiation and apoptosis. In particular it plays a significant role in bone development and osteogenesis.

Pathways

Smad1 plays an important role within the BMP signaling pathway which is important for early development and tissue homeostasis. Within this pathway BMPs trigger the phosphorylation of Smad1 which then associates with Smad4 to propagate downstream signaling. Another associated pathway includes the TGF-beta signaling pathway where Smads like Smad2 and Smad3 show functional similarities and differences with Smad1. The interactions of Smad1 with related proteins like Smad4 highlight its significant contribution to cellular processes regulated by these pathways.

Smad1 associates with various pathological conditions. For instance aberrant Smad1 signaling has implications in cancer particularly in processes like tumor progression and metastasis. Disruptions in the BMP pathway involving Smad1 and Smad4 can influence the onset and development of disorders such as pulmonary hypertension where inappropriate cell growth and remodeling occur. Understanding Smad1's role in these contexts provides insights into potential therapeutic avenues for treatment and management of these conditions.

style

left-column

style

left-column

Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

style

left-column

Target data

Transcriptional modulator that plays a role in various cellular processes, including embryonic development, cell differentiation, and tissue homeostasis (PubMed : 9335504). Upon BMP ligand binding to their receptors at the cell surface, is phosphorylated by activated type I BMP receptors (BMPRIs) and associates with SMAD4 to form a heteromeric complex which translocates into the nucleus acting as transcription factor (PubMed : 33667543). In turn, the hetero-trimeric complex recognizes cis-regulatory elements containing Smad Binding Elements (SBEs) to modulate the outcome of the signaling network (PubMed : 33667543). SMAD1/OAZ1/PSMB4 complex mediates the degradation of the CREBBP/EP300 repressor SNIP1. Positively regulates BMP4-induced expression of odontogenic development regulator MSX1 following IPO7-mediated nuclear import (By similarity).

See full target information SMAD1 phospho S463 + S465

style

left-column

Publications (5)

Recent publications for all applications. Explore the full list and refine your search

Haematologica 109:186-199 PubMed37534528

2024

Hypomethylating agent decitabine sensitizes diffuse large B-cell lymphoma to venetoclax.

Applications

Unspecified application

Species

Unspecified reactive species

Fen Zhu,Jennifer L Crombie,Wei Ni,Nguyet-Minh Hoang,Swati Garg,Liam Hackett,Stephen J F Chong,Mary C Collins,Lixin Rui,James Griffin,Matthew S Davids

Frontiers in cell and developmental biology 11:1158936 PubMed37283947

2023

Establishing and characterizing human stem cells from the apical papilla immortalized by hTERT gene transfer.

Applications

Unspecified application

Species

Unspecified reactive species

Qianyu Cheng,Chang Liu,Qiuman Chen,Wenping Luo,Tong-Chuan He,Deqin Yang

The Journal of clinical investigation 131: PubMed34779414

2021

Candesartan prevents arteriopathy progression in cerebral autosomal recessive arteriopathy with subcortical infarcts and leukoencephalopathy model.

Applications

Unspecified application

Species

Unspecified reactive species

Taisuke Kato,Ri-Ichiroh Manabe,Hironaka Igarashi,Fuyuki Kametani,Sachiko Hirokawa,Yumi Sekine,Natsumi Fujita,Satoshi Saito,Yusuke Kawashima,Yuya Hatano,Shoichiro Ando,Hiroaki Nozaki,Akihiro Sugai,Masahiro Uemura,Masaki Fukunaga,Toshiya Sato,Akihide Koyama,Rie Saito,Atsushi Sugie,Yasuko Toyoshima,Hirotoshi Kawata,Shigeo Murayama,Masaki Matsumoto,Akiyoshi Kakita,Masato Hasegawa,Masafumi Ihara,Masato Kanazawa,Masatoyo Nishizawa,Shoji Tsuji,Osamu Onodera

Cancers 12: PubMed32731498

2020

TrkB Inhibits the BMP Signaling-Mediated Growth Inhibition of Cancer Cells.

Applications

Unspecified application

Species

Unspecified reactive species

Min Soo Kim,Wook Jin

Advanced science (Weinheim, Baden-Wurttemberg, Ger 5:1800873 PubMed30356983

2018

Contribution of Mitophagy to Cell-Mediated Mineralization: Revisiting a 50-Year-Old Conundrum.

Applications

Unspecified application

Species

Unspecified reactive species

Dan-Dan Pei,Jin-Long Sun,Chun-Hui Zhu,Fu-Cong Tian,Kai Jiao,Matthew R Anderson,Cynthia Yiu,Cui Huang,Chang-Xiong Jin,Brian E Bergeron,Ji-Hua Chen,Franklin R Tay,Li-Na Niu

View all publications

style

left-column

style

left-column

style

right-column

Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.

For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com

Anti-Smad1 (phospho S463 + S465) antibody [EPR20662-29]

Western blot - Anti-Smad1 (phospho S463 + S465) antibody [EPR20662-29] (AB214423)

Immunoprecipitation - Anti-Smad1 (phospho S463 + S465) antibody [EPR20662-29] (AB214423)

Dot Blot - Anti-Smad1 (phospho S463 + S465) antibody [EPR20662-29] (AB214423)

Western blot - Anti-Smad1 (phospho S463 + S465) antibody [EPR20662-29] (AB214423)

Related conjugates and formulations (1)

Key facts

Host species

Clonality

Clone number

Isotype

Carrier free

Reacts with

Applications

Immunogen

Specificity

Reactivity data

Product details

Properties and storage information

Form

Purification technique

Storage buffer

Shipped at conditions

Appropriate short-term storage duration

Appropriate short-term storage conditions

Appropriate long-term storage conditions

Aliquoting information

Storage information

Supplementary information

Biological function summary

Pathways

Product protocols

Target data

Publications (5)

Alternative Products

Primary Antibodies

Primary Antibodies

Primary Antibodies

Primary Antibodies

Primary Antibodies

Primary Antibodies

Primary Antibodies

Primary Antibodies

Primary Antibodies

Primary Antibodies

Primary Antibodies

Primary Antibodies

Complementary Products

Assay Kits

Secondary Antibodies

Primary Antibodies

Primary Antibodies

Primary Antibodies

Product promise