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AB228765

Anti-Smad2 antibody

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(6 Publications)

Rabbit Polyclonal SMAD2 antibody. Suitable for WB, IHC-P, ICC/IF and reacts with Human, Mouse, Rat samples. Cited in 6 publications. Immunogen corresponding to Recombinant Fragment Protein within Human SMAD2.

View Alternative Names

MADH2, MADR2, SMAD2, SMAD family member 2, SMAD 2, hSMAD2, JV18-1, Mad-related protein 2, Mothers against decapentaplegic homolog 2, hMAD-2, MAD homolog 2, Mothers against DPP homolog 2

4 Images
Immunocytochemistry/ Immunofluorescence - Anti-Smad2 antibody (AB228765)
  • ICC/IF

Supplier Data

Immunocytochemistry/ Immunofluorescence - Anti-Smad2 antibody (AB228765)

Paraformaldehyde-fixed HeLa (human epithelial cell line from cervix adenocarcinoma) cells stained for Smad2 (green) using ab228765 at 1/500 dilution in ICC/IF.

Alpha-tubulin filaments were labeled with an anti-Alpha-tubulin antibody at 1/2000 dilution (red).

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Smad2 antibody (AB228765)
  • IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Smad2 antibody (AB228765)

Paraffin-embedded rat testis tissue stained for Smad2 using ab228765 at 1/500 dilution in immunohistochemical analysis.

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Smad2 antibody (AB228765)
  • IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Smad2 antibody (AB228765)

Paraffin-embedded mouse liver tissue stained for Smad2 using ab228765 at 1/500 dilution in immunohistochemical analysis.

Western blot - Anti-Smad2 antibody (AB228765)
  • WB

Supplier Data

Western blot - Anti-Smad2 antibody (AB228765)

10% SDS-PAGE gel.

All lanes:

Western blot - Anti-Smad2 antibody (ab228765) at 1/500 dilution

All lanes:

Jurkat (human T cell leukemia cell line from peripheral blood) whole cell lysate

Predicted band size: 52 kDa

false

Key facts

Host species

Rabbit

Clonality

Polyclonal

Isotype

IgG

Carrier free

No

Reacts with

Mouse, Rat, Human

Applications

ICC/IF, WB, IHC-P

applications

Immunogen

Recombinant Fragment Protein within Human SMAD2. The exact immunogen used to generate this antibody is proprietary information.

Q15796

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Reactivity data

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Properties and storage information

Form
Liquid
Purification technique
Affinity purification Immunogen
Storage buffer
pH: 7 Preservative: 0.01% Thimerosal (merthiolate) Constituents: 20% Glycerol (glycerin, glycerine), 1.21% Tris, 0.75% Glycine
Shipped at conditions
Blue Ice
Appropriate short-term storage duration
1-2 weeks
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
-20°C
Aliquoting information
Upon delivery aliquot
Storage information
Avoid freeze / thaw cycle
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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

Smad2 also known as Mothers against decapentaplegic homolog 2 (MAD2) or MADR2 is a signaling protein involved in the transforming growth factor-beta (TGF-β) receptor pathway. Smad2 has a molecular mass of approximately 58 kDa and expresses in various tissues including epithelial mesenchymal and endothelial cells. Smad2 undergoes phosphorylation on serine residues in response to TGF-β signaling converting it into phosphorylated forms often referred to as p-Smad2 or phospho-Smad2. These phosphorylated forms are critical for the relay of signals from the cell surface to the nucleus.
Biological function summary

Smad2 acts as an intracellular mediator for TGF-β signaling a pathway important for regulating cell proliferation differentiation and apoptosis. Smad2 typically functions as part of a heteromeric complex with Smad4 another key player in TGF-β signaling. Upon activation phosphorylated Smad2 combines with Smad4 to form a complex that translocates into the nucleus. This complex then binds to specific DNA sequences to regulate the transcription of target genes involved in processes such as cell growth inhibition and extracellular matrix production.

Pathways

Smad2 is integral to the TGF-β and activin receptor signaling pathways. These pathways are essential in controlling cell growth and immune responses. Smad2 interacts with other proteins such as Smad3 in addition to Smad4 to modulate gene expression effectively. The interaction between Smad2 and these proteins ensures precise control of cellular responses to external stimuli emphasizing its pivotal role in maintaining cellular homeostasis.

Smad2 correlates with various pathological conditions including fibrosis and cancer. Aberrant Smad2 signaling can contribute to the development of these diseases as excessive TGF-β signaling promotes fibrotic tissue deposition and tumor progression. Smad2 connects with other proteins like Smad3 in these pathological contexts both acting as mediators of abnormal cellular behaviors. Understanding the regulatory mechanisms of Smad2 can help develop therapeutic strategies against disorders linked to dysregulated TGF-β signaling.
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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:
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Target data

Receptor-regulated SMAD (R-SMAD) that is an intracellular signal transducer and transcriptional modulator activated by TGF-beta (transforming growth factor) and activin type 1 receptor kinases. Binds the TRE element in the promoter region of many genes that are regulated by TGF-beta and, on formation of the SMAD2/SMAD4 complex, activates transcription. Promotes TGFB1-mediated transcription of odontoblastic differentiation genes in dental papilla cells (By similarity). Positively regulates PDPK1 kinase activity by stimulating its dissociation from the 14-3-3 protein YWHAQ which acts as a negative regulator. May act as a tumor suppressor in colorectal carcinoma (PubMed : 8752209).
See full target information SMAD2
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Publications (6)

Recent publications for all applications. Explore the full list and refine your search

Cancer cell international 25:179 PubMed40380175

2025

Fibrogenesis-driven tumor progression in clear cell renal cell carcinoma: prognostic, therapeutic implications and the dual role of neuropilin-1.

Applications

Unspecified application

Species

Unspecified reactive species

Kai Wang,Xihao Shen,Jiyue Wu,Qing Bi,Zihao Gao,Zejia Sun,Wei Wang

Annals of surgical oncology 30:8690-8703 PubMed37598115

2023

LncRNA EBLN3P Facilitates Osteosarcoma Metastasis by Enhancing Annexin A3 mRNA Stability and Recruiting HuR.

Applications

Unspecified application

Species

Unspecified reactive species

Shengtao Wang,Xinxin Zeng,Peng Gui,Shujuan Xu,Zhaoxu Li,Dongxu Chen

Pharmacology 108:432-443 PubMed37343534

2023

Quercetin Alleviates Asthma-Induced Airway Inflammation and Remodeling through Downregulating Periostin via Blocking TGF-β1/Smad Pathway.

Applications

Unspecified application

Species

Unspecified reactive species

Yanni Fang,Wenwen Jin,Zhen Guo,Jumei Hao

American journal of translational research 15:151-164 PubMed36777827

2023

Protocatechuic acid, the main effective monomer in Wuqi Powder, can inhibit gastric ulcers induced by acetic acid and .

Applications

Unspecified application

Species

Unspecified reactive species

Yuanyuan Wang,Liang Zheng

Antioxidants (Basel, Switzerland) 11: PubMed36290678

2022

MicroRNA-4732-3p Is Dysregulated in Breast Cancer Patients with Cardiotoxicity, and Its Therapeutic Delivery Protects the Heart from Doxorubicin-Induced Oxidative Stress in Rats.

Applications

Unspecified application

Species

Unspecified reactive species

Rafael Sánchez-Sánchez,Ignacio Reinal,Esteban Peiró-Molina,Marc Buigues,Sandra Tejedor,Amparo Hernándiz,Marta Selva,David Hervás,Antonio J Cañada,Akaitz Dorronsoro,Ana Santaballa,Carmen Salvador,Florian Caiment,Jos Kleinjans,Luis Martínez-Dolz,Isabel Moscoso,Ricardo Lage,José R González-Juanatey,Joaquín Panadero,Ernesto Aparicio-Puerta,Antonio Bernad,Pilar Sepúlveda

Allergologia et immunopathologia 49:64-71 PubMed34476924

2021

MiR-216a-5p alleviates LPS-induced inflammation in the human bronchial epithelial cell by inhibition of TGF-β1 signaling via down-regulating TGFBR2.

Applications

Unspecified application

Species

Unspecified reactive species

Shan Liu,Jianjun Li,Liya Hu
View all publications
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