AB63576

Anti-Smad2 antibody

Name: Anti-Smad2 antibody (ab63576) | Abcam
Brand: Abcam Limited
SKU: AB63576
Price: 565 USD
Availability: InStock
Rating: 4 (8 reviews)

(8 Reviews)

(40 Publications)

Rabbit Polyclonal SMAD2 antibody. Suitable for IHC-P, ICC/IF and reacts with Human samples. Cited in 40 publications. Immunogen corresponding to Synthetic Peptide within Human SMAD2.

View Alternative Names

MADH2, MADR2, SMAD2, SMAD family member 2, SMAD 2, hSMAD2, JV18-1, Mad-related protein 2, Mothers against decapentaplegic homolog 2, hMAD-2, MAD homolog 2, Mothers against DPP homolog 2

3 Images

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Smad2 antibody (AB63576)

IHC-P

Unknown

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Smad2 antibody (AB63576)

ab63576, at a 1/50 dilution, staining human Smad2 in prostate carcinoma, using Immunohistochemistry, Formalin/PFA fixed Paraffin embedded tissue, minus immunising peptide (left image) and plus immunising peptide (right image).

Immunocytochemistry/ Immunofluorescence - Anti-Smad2 antibody (AB63576)

ICC/IF

Supplier Data

Immunocytochemistry/ Immunofluorescence - Anti-Smad2 antibody (AB63576)

Immunofluorescent staining of HepG2 cells, using ab63576 at a 1/500 dilution, minus immunising peptide (left image) and plus immunising peptide (right image).

ICC/IF

AbReview20101****

Immunocytochemistry/ Immunofluorescence - Anti-Smad2 antibody (AB63576)

ab63576 staining Smad2 in Mouse enterocytes by ICC/IF (Immunocytochemistry/immunofluorescence). Cells were fixed with paraformaldehyde, permeabilized with 0.5% Triton and blocked with 5% BSA for 1 hour at 20°C. Samples were incubated with primary antibody (1/250) for 2 hours at 20°C. An Alexa Fluor^®488-conjugated Goat anti-rabbit IgG polyclonal (1/1000) was used as the secondary antibody.

This image is courtesy of an anonymous Abreview

Key facts

Host species

Rabbit

Clonality

Polyclonal

Isotype

IgG

Carrier free

Reacts with

Human

Applications

IHC-P, ICC/IF

applications

Immunogen

Synthetic Peptide within Human SMAD2. The exact immunogen used to generate this antibody is proprietary information.

Q15796

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"}, "ICCIF" : {"fullname" : "Immunocytochemistry/ Immunofluorescence", "shortname":"ICC/IF"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "1/50 - 1/100", "IHCP-species-notes": "<p></p>", "ICCIF-species-checked": "testedAndGuaranteed", "ICCIF-species-dilution-info": "1/500 - 1/1000", "ICCIF-species-notes": "<p></p>" } } }

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Properties and storage information

Form

Liquid

Purification technique

Affinity purification Immunogen

Storage buffer

pH: 7.4 Preservative: 0.02% Sodium azide Constituents: PBS, 50% Glycerol (glycerin, glycerine), 0.87% Sodium chloride

Shipped at conditions

Blue Ice

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

-20°C

Storage information

Stable for 12 months at -20°C

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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

Smad2 also known as Mothers against decapentaplegic homolog 2 (MAD2) or MADR2 is a signaling protein involved in the transforming growth factor-beta (TGF-β) receptor pathway. Smad2 has a molecular mass of approximately 58 kDa and expresses in various tissues including epithelial mesenchymal and endothelial cells. Smad2 undergoes phosphorylation on serine residues in response to TGF-β signaling converting it into phosphorylated forms often referred to as p-Smad2 or phospho-Smad2. These phosphorylated forms are critical for the relay of signals from the cell surface to the nucleus.

Biological function summary

Smad2 acts as an intracellular mediator for TGF-β signaling a pathway important for regulating cell proliferation differentiation and apoptosis. Smad2 typically functions as part of a heteromeric complex with Smad4 another key player in TGF-β signaling. Upon activation phosphorylated Smad2 combines with Smad4 to form a complex that translocates into the nucleus. This complex then binds to specific DNA sequences to regulate the transcription of target genes involved in processes such as cell growth inhibition and extracellular matrix production.

Pathways

Smad2 is integral to the TGF-β and activin receptor signaling pathways. These pathways are essential in controlling cell growth and immune responses. Smad2 interacts with other proteins such as Smad3 in addition to Smad4 to modulate gene expression effectively. The interaction between Smad2 and these proteins ensures precise control of cellular responses to external stimuli emphasizing its pivotal role in maintaining cellular homeostasis.

Smad2 correlates with various pathological conditions including fibrosis and cancer. Aberrant Smad2 signaling can contribute to the development of these diseases as excessive TGF-β signaling promotes fibrotic tissue deposition and tumor progression. Smad2 connects with other proteins like Smad3 in these pathological contexts both acting as mediators of abnormal cellular behaviors. Understanding the regulatory mechanisms of Smad2 can help develop therapeutic strategies against disorders linked to dysregulated TGF-β signaling.

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

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Target data

Receptor-regulated SMAD (R-SMAD) that is an intracellular signal transducer and transcriptional modulator activated by TGF-beta (transforming growth factor) and activin type 1 receptor kinases. Binds the TRE element in the promoter region of many genes that are regulated by TGF-beta and, on formation of the SMAD2/SMAD4 complex, activates transcription. Promotes TGFB1-mediated transcription of odontoblastic differentiation genes in dental papilla cells (By similarity). Positively regulates PDPK1 kinase activity by stimulating its dissociation from the 14-3-3 protein YWHAQ which acts as a negative regulator. May act as a tumor suppressor in colorectal carcinoma (PubMed : 8752209).

See full target information SMAD2

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Publications (40)

Recent publications for all applications. Explore the full list and refine your search

Antonie van Leeuwenhoek 116:565-576 PubMed37186068

2023

Tetrahydrocurcumin (THC) enhanced the clearance of Cryptococcus deneoformans during infection in vivo.

Applications

Unspecified application

Species

Unspecified reactive species

Tianli He,Zhiran Jin,Wei Hu,Xiaoxue Xia,Donghui Li,Weiyun Yao,Guangnan Li,Xuefeng Zhou,Guoqiang Song

Biomaterials research 26:78 PubMed36514131

2022

Biomimetic hydrogel blanket for conserving and recovering intrinsic cell properties.

Applications

Unspecified application

Species

Unspecified reactive species

Seung-Hoon Um,Youngmin Seo,Hyunseon Seo,Kyungwoo Lee,Sun Hwa Park,Jung Ho Jeon,Jung Yeon Lim,Myoung-Ryul Ok,Yu-Chan Kim,Hyunjung Kim,Cheol-Hong Cheon,Hyung-Seop Han,James R Edwards,Sung Won Kim,Hojeong Jeon

Oxidative medicine and cellular longevity 2022:8770136 PubMed36193084

2022

Early Development of Cardiac Fibrosis in Young Old-Father Offspring.

Applications

Unspecified application

Species

Unspecified reactive species

A Ismail,Y Saliba,N Fares

Cells 10: PubMed34944010

2021

Vardenafil Activity in Lung Fibrosis and In Vitro Synergy with Nintedanib.

Applications

Unspecified application

Species

Unspecified reactive species

Michael H Bourne,Theodore J Kottom,Deanne M Hebrink,Malay Choudhury,Edward B Leof,Andrew H Limper

American journal of translational research 13:10206-10217 PubMed34650691

2021

Promotion of chemerin in rat diabetic kidney disease through enhancement of TGF-β1/Smads/CTGF pathway.

Applications

Unspecified application

Species

Unspecified reactive species

Weiwei Wang,Jun Guo,Dongqiang Wang

Nature communications 12:4980 PubMed34404792

2021

Spatiotemporally-resolved mapping of RNA binding proteins via functional proximity labeling reveals a mitochondrial mRNA anchor promoting stress recovery.

Applications

Unspecified application

Species

Unspecified reactive species

Wei Qin,Samuel A Myers,Dominique K Carey,Steven A Carr,Alice Y Ting

Evidence-based complementary and alternative medicine : eCAM 2021:5649575 PubMed34422075

2021

Antifibrotic Effects of Kangxian Ruangan Capsule on Rats with Nonalcoholic Fatty Liver Fibrosis and Hepatic Stellate Cells through Regulation of TGF- and TLR4 Signaling Pathways.

Applications

Unspecified application

Species

Unspecified reactive species

Liming Liu,Ying Zhou,Dan Dai,Hongmei Xia,Kang Zhao,Jianjun Zhang

Molecular medicine reports 24: PubMed34328199

2021

Liquiritigenin attenuates isoprenaline‑induced myocardial fibrosis in mice through the TGF‑β1/Smad2 and AKT/ERK signaling pathways.

Applications

Unspecified application

Species

Unspecified reactive species

Li Li,Hui Fang,Yong-Hong Yu,Shan-Xin Liu,Zhi-Qiang Yang

Journal of equine science 32:39-48 PubMed34220270

2021

Circulating activin A during equine gestation and immunolocalization of its receptors system in utero-placental tissues and fetal gonads.

Applications

Unspecified application

Species

Unspecified reactive species

Pramod Dhakal,Nobuo Tsunoda,Yasuo Nambo,Hiroyuki Taniyama,Kentaro Nagaoka,Gen Watanabe,Kazuyoshi Taya

Frontiers in cell and developmental biology 8:568304 PubMed33763412

2021

Upregulation of Extracellular Vesicles-Encapsulated miR-132 Released From Mesenchymal Stem Cells Attenuates Ischemic Neuronal Injury by Inhibiting Smad2/c-jun Pathway Acvr2b Suppression.

Applications

Unspecified application

Species

Unspecified reactive species

Bin Feng,Lei Meng,Liming Luan,Zhihao Fang,Peng Zhao,Guangyu Zhao

View all publications

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Product promise

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