AB300080

Anti-Smad2 (phospho S250) antibody [EPR26263-58] (BSA and Azide free)

RabMAb
Recombinant
KO Validated
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Rabbit Recombinant Monoclonal SMAD2 phospho S250 antibody. Carrier free. Suitable for WB, ICC/IF, IP, Dot and reacts with Rat, Mouse, Human, Synthetic peptide samples.

View Alternative Names

MADH2, MADR2, SMAD2, Mothers against decapentaplegic homolog 2, MAD homolog 2, Mothers against DPP homolog 2, JV18-1, Mad-related protein 2, SMAD family member 2, hMAD-2, SMAD 2, Smad2, hSMAD2

16 Images

Immunocytochemistry/ Immunofluorescence - Anti-Smad2 (phospho S250) antibody [EPR26263-58] (BSA and Azide free) (AB300080)

ICC/IF

Supplier Data

Immunocytochemistry/ Immunofluorescence - Anti-Smad2 (phospho S250) antibody [EPR26263-58] (BSA and Azide free) (AB300080)

This data was developed using ab300079, the same antibody clone in a different buffer formulation.

Immunofluorescent analysis of 4% Paraformaldehyde-fixed, 0.1% TritonX-100 permeabilized HeLa (human cervix adenocarcinoma epithelial cell) cells lebeling Smad2 (phospho S250) with ab300079 at 1/50 (10.8 μg/ml) dilution, followed by ab150081 Goat Anti-Rabbit IgG H&L (Alexa Fluor^® 488) preadsorbed antibody at 1/1000 (2 μg/mL) dilution (Green). Confocal image showing increased cytoplasm and nuclear staining in HeLa cells treated with PMA (200 nM ) for 30 min. ab195889 Anti-alpha Tubulin mouse monoclonal antibody - Microtubule Marker (Alexa Fluor^® 594) was used to counterstain tubulin at 1/200 (2.5 μg/ml) dilution (Red). The Nuclear counterstain was DAPI (Blue).

Secondary antibody only control : Secondary antibody is ab150081 Goat Anti-Rabbit IgG H&L (Alexa Fluor^® 488) preadsorbed at 1/1000 (2 μg/mL) dilution.

ICC/IF

Supplier Data

Immunocytochemistry/ Immunofluorescence - Anti-Smad2 (phospho S250) antibody [EPR26263-58] (BSA and Azide free) (AB300080)

This data was developed using ab300079, the same antibody clone in a different buffer formulation. Immunofluorescent analysis of 4% Paraformaldehyde-fixed, 0.1% TritonX-100 permeabilized HeLa (human cervix adenocarcinoma epithelial cell) cells lebeling Smad2 (phospho S250) with ab300079 at 1/50 (10.8 µg/ml) dilution, followed by ab150081 Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed antibody at 1/1000 (2 µg/mL) dilution (Green). Confocal image showing increased cytoplasm and nuclear staining in HeLa cells treated with PMA (200 nM ) for 30 min. ab195889 Anti-alpha Tubulin mouse monoclonal antibody - Microtubule Marker (Alexa Fluor® 594) was used to counterstain tubulin at 1/200 (2.5 µg/ml) dilution (Red). The Nuclear counterstain was DAPI (Blue). Secondary antibody only control : Secondary antibody is ab150081 Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed at 1/1000 (2 µg/mL) dilution.

Supplier Data

Immunoprecipitation - Anti-Smad2 (phospho S250) antibody [EPR26263-58] (BSA and Azide free) (AB300080)

This data was developed using ab300079, the same antibody clone in a different buffer formulation.

Smad2 (phospho S250) was immunoprecipitated from 0.35 mg HeLa (human cervix adenocarcinoma epithelial cell) treated with 0.2μM PMA for 0.5h whole cell lysate with ab300079 at 1/30 dilution (2 μg in 0.35 mg lysates). Western blot was performed on the immunoprecipitate using ab300079 at 1/1000 dilution. VeriBlot for IP secondary antibody (HRP)(ab131366) was used at 1/5000 dilution.

Lane 1 : HeLa (human cervix adenocarcinoma epithelial cell) treated with 0.2μM PMA for 0.5h whole cell lysate 10 μg (Inset)

Lane 2 : ab300079 in HeLa treated with 0.2μM PMA for 0.5h whole cell lysate

Lane 3 : Rabbit monoclonal IgG (ab172730) instead of ab300079 in HeLa treated with 0.2μM PMA for 0.5h whole cell lysate

Blocking and dilution buffer and concentration : 5% NFDM/TBST.

Exposure time : 10 seconds

All lanes:

Immunoprecipitation - Anti-Smad2 (phospho S250) antibody [EPR26263-58] (<a href='/en-us/products/primary-antibodies/smad2-phospho-s250-antibody-epr26263-58-ab300079'>ab300079</a>)

Predicted band size: 52 kDa

Observed band size: 65 kDa

false

Supplier Data

Immunoprecipitation - Anti-Smad2 (phospho S250) antibody [EPR26263-58] (BSA and Azide free) (AB300080)

This data was developed using ab300079, the same antibody clone in a different buffer formulation. Smad2 (phospho S250) was immunoprecipitated from 0.35 mg HeLa (human cervix adenocarcinoma epithelial cell) treated with 0.2µM PMA for 0.5h whole cell lysate with ab300079 at 1/30 dilution (2 µg in 0.35 mg lysates). Western blot was performed on the immunoprecipitate using ab300079 at 1/1000 dilution. VeriBlot for IP secondary antibody (HRP)(ab131366) was used at 1/5000 dilution. Lane 1 : HeLa (human cervix adenocarcinoma epithelial cell) treated with 0.2µM PMA for 0.5h whole cell lysate 10 µg (Inset) Lane 2 : ab300079 in HeLa treated with 0.2µM PMA for 0.5h whole cell lysate Lane 3 : Rabbit monoclonal IgG (ab172730) instead of ab300079 in HeLa treated with 0.2µM PMA for 0.5h whole cell lysate Blocking and dilution buffer and concentration : 5% NFDM/TBST. Exposure time : 10 seconds

All lanes:

Immunoprecipitation - Anti-Smad2 (phospho S250) antibody [EPR26263-58] (<a href='/en-us/products/primary-antibodies/smad2-phospho-s250-antibody-epr26263-58-ab300079'>ab300079</a>) at 1/1000 dilution

All lanes:

HeLa (human cervix adenocarcinoma epithelial cell) treated with 0.2µM PMA for 0.5h whole cell lysate at 10 µg

Secondary

All lanes:

Immunoprecipitation - VeriBlot for IP Detection Reagent (HRP) (<a href='/en-us/products/reagents/veriblot-for-ip-detection-reagent-hrp-ab131366'>ab131366</a>) at 1/5000 dilution

Predicted band size: 52 kDa

Observed band size: 65 kDa

false

Exposure time: 10s

Supplier Data

Immunoprecipitation - Anti-Smad2 (phospho S250) antibody [EPR26263-58] (BSA and Azide free) (AB300080)

This data was developed using ab300079, the same antibody clone in a different buffer formulation. Smad2 (phospho S250) was immunoprecipitated from 0.35 mg NIH/3T3 treated with 0.2µM PMA for 0.5h whole cell lysate with ab300079 at 1/30 dilution (2µg in 0.35mg lysates). Western blot was performed on the immunoprecipitate using ab300079 at 1/1000 dilution. VeriBlot for IP secondary antibody (HRP)(ab131366) was used at 1/5000 dilution. Lane 1 : NIH/3T3 treated with 0.2µM PMA for 0.5h whole cell lysate 20 µg (Inset) Lane 2 : ab300079 in NIH/3T3 treated with 0.2µM PMA for 0.5h whole cell lysate Lane 3 : Rabbit monoclonal IgG (ab172730) instead of ab300079 in NIH/3T3 whole cell lysate Blocking and dilution buffer and concentration : 5% NFDM/TBST. Exposure time : 10 seconds

All lanes:

Immunoprecipitation - Anti-Smad2 (phospho S250) antibody [EPR26263-58] (<a href='/en-us/products/primary-antibodies/smad2-phospho-s250-antibody-epr26263-58-ab300079'>ab300079</a>)

All lanes:

NIH/3T3 treated with 0.2µM PMA for 0.5h whole cell lysate at 20 µg

Secondary

All lanes:

Immunoprecipitation - VeriBlot for IP Detection Reagent (HRP) (<a href='/en-us/products/reagents/veriblot-for-ip-detection-reagent-hrp-ab131366'>ab131366</a>) at 1/5000 dilution

Predicted band size: 52 kDa

Observed band size: 65 kDa

false

Exposure time: 10s

Supplier Data

Immunoprecipitation - Anti-Smad2 (phospho S250) antibody [EPR26263-58] (BSA and Azide free) (AB300080)

This data was developed using ab300079, the same antibody clone in a different buffer formulation.

Smad2 (phospho S250) was immunoprecipitated from 0.35 mg NIH/3T3 treated with 0.2μM PMA for 0.5h whole cell lysate with ab300079 at 1/30 dilution (2μg in 0.35mg lysates). Western blot was performed on the immunoprecipitate using ab300079 at 1/1000 dilution. VeriBlot for IP secondary antibody (HRP)(ab131366) was used at 1/5000 dilution.

Lane 1 : NIH/3T3 treated with 0.2μM PMA for 0.5h whole cell lysate 20 μg (Inset)

Lane 2 : ab300079 in NIH/3T3 treated with 0.2μM PMA for 0.5h whole cell lysate

Lane 3 : Rabbit monoclonal IgG (ab172730) instead of ab300079 in NIH/3T3 whole cell lysate

Blocking and dilution buffer and concentration : 5% NFDM/TBST.

Exposure time : 10 seconds

All lanes:

Immunoprecipitation - Anti-Smad2 (phospho S250) antibody [EPR26263-58] (<a href='/en-us/products/primary-antibodies/smad2-phospho-s250-antibody-epr26263-58-ab300079'>ab300079</a>)

Predicted band size: 52 kDa

Observed band size: 65 kDa

false

Supplier Data

Western blot - Anti-Smad2 (phospho S250) antibody [EPR26263-58] (BSA and Azide free) (AB300080)

This data was developed using ab300079, the same antibody clone in a different buffer formulation. 5% NFDM/TBST was used as blocking and diluting buffer.

All lanes:

Western blot - Anti-Smad2 (phospho S250) antibody [EPR26263-58] (<a href='/en-us/products/primary-antibodies/smad2-phospho-s250-antibody-epr26263-58-ab300079'>ab300079</a>) at 1/1000 dilution

Lane 1:

Untreated parental HeLa (human cervix adenocarcinoma epithelial cell) whole cell lysate at 30 µg

Lane 2:

Parental HeLa treated with 0.2µM PMA for 0.5h whole cell lysate at 30 µg

Lane 3:

Untreated SAMD2 KO Hela whole cell lysate at 30 µg

Lane 4:

SAMD2 KO Hela treated with 0.2µM PMA for 0.5h whole cell lysate at 30 µg

Secondary

All lanes:

Western blot - Goat Anti-Rabbit IgG H&L (HRP) (<a href='/en-us/products/secondary-antibodies/goat-rabbit-igg-h-l-hrp-ab97051'>ab97051</a>) at 1/20000 dilution

Predicted band size: 52 kDa

Observed band size: 65 kDa

false

Exposure time: 10s

Supplier Data

Western blot - Anti-Smad2 (phospho S250) antibody [EPR26263-58] (BSA and Azide free) (AB300080)

This data was developed using ab300079, the same antibody clone in a different buffer formulation. 5% NFDM/TBST was used as blocking and diluting buffer.

All lanes:

Western blot - Anti-Smad2 (phospho S250) antibody [EPR26263-58] (<a href='/en-us/products/primary-antibodies/smad2-phospho-s250-antibody-epr26263-58-ab300079'>ab300079</a>) at 1/1000 dilution

Lane 1:

HeLa (human cervix adenocarcinoma epithelial cell) treated with 0.2µM PMA for 0.5h (Untreated membrane) whole cell lysate at 20 µg

Lane 2:

HeLa treated with 0.2µM PMA for 0.5h (phosphatase treated membrane) whole cell lysate at 20 µg

Secondary

All lanes:

Western blot - Goat Anti-Rabbit IgG H&L (HRP) (<a href='/en-us/products/secondary-antibodies/goat-rabbit-igg-h-l-hrp-ab97051'>ab97051</a>) at 1/100000 dilution

Predicted band size: 52 kDa

Observed band size: 65 kDa

false

Exposure time: 180s

Supplier Data

Western blot - Anti-Smad2 (phospho S250) antibody [EPR26263-58] (BSA and Azide free) (AB300080)

This data was developed using ab300079, the same antibody clone in a different buffer formulation.

5% NFDM/TBST was used as blocking and diluting buffer.

All lanes:

Western blot - Anti-Smad2 (phospho S250) antibody [EPR26263-58] (<a href='/en-us/products/primary-antibodies/smad2-phospho-s250-antibody-epr26263-58-ab300079'>ab300079</a>) at 1/1000 dilution

Lane 1:

Untreated parental HeLa (human cervix adenocarcinoma epithelial cell) whole cell lysate at 30 µg

Lane 2:

Parental HeLa treated with 0.2µM PMA for 0.5h whole cell lysate at 30 µg

Lane 3:

Untreated SAMD2 KO Hela whole cell lysate at 30 µg

Lane 4:

SAMD2 KO Hela treated with 0.2µM PMA for 0.5h whole cell lysate at 30 µg

Secondary

All lanes:

Western blot - Goat Anti-Rabbit IgG H&L (HRP) (<a href='/en-us/products/secondary-antibodies/goat-rabbit-igg-h-l-hrp-ab97051'>ab97051</a>) at 1/20000 dilution

Predicted band size: 52 kDa

Observed band size: 62 kDa,65 kDa

false

Exposure time: 10s

Supplier Data

Western blot - Anti-Smad2 (phospho S250) antibody [EPR26263-58] (BSA and Azide free) (AB300080)

This data was developed using ab300079, the same antibody clone in a different buffer formulation.

5% NFDM/TBST was used as blocking and diluting buffer.

All lanes:

Western blot - Anti-Smad2 (phospho S250) antibody [EPR26263-58] (<a href='/en-us/products/primary-antibodies/smad2-phospho-s250-antibody-epr26263-58-ab300079'>ab300079</a>) at 1/1000 dilution

Lane 1:

HeLa (human cervix adenocarcinoma epithelial cell) treated with 0.2µM PMA for 0.5h (Untreated membrane) whole cell lysate at 20 µg

Lane 2:

HeLa treated with 0.2µM PMA for 0.5h (phosphatase treated membrane) whole cell lysate at 20 µg

Secondary

All lanes:

Western blot - Goat Anti-Rabbit IgG H&L (HRP) (<a href='/en-us/products/secondary-antibodies/goat-rabbit-igg-h-l-hrp-ab97051'>ab97051</a>) at 1/100000 dilution

Predicted band size: 52 kDa

Observed band size: 65 kDa

false

Exposure time: 180s

Supplier Data

Western blot - Anti-Smad2 (phospho S250) antibody [EPR26263-58] (BSA and Azide free) (AB300080)

This data was developed using ab300079, the same antibody clone in a different buffer formulation.

5% NFDM/TBST was used as blocking and diluting buffer.

All lanes:

Western blot - Anti-Smad2 (phospho S250) antibody [EPR26263-58] (<a href='/en-us/products/primary-antibodies/smad2-phospho-s250-antibody-epr26263-58-ab300079'>ab300079</a>) at 1/1000 dilution

Lane 1:

Untreated NIH/3T3 (Mouse embryonic fibroblast) whole cell lysate at 20 µg

Lane 2:

NIH/3T3 treated with 0.2µM PMA for 0.5h whole cell lysate at 20 µg

Secondary

All lanes:

Western blot - Goat Anti-Rabbit IgG H&L (HRP) (<a href='/en-us/products/secondary-antibodies/goat-rabbit-igg-h-l-hrp-ab97051'>ab97051</a>) at 1/100000 dilution

Predicted band size: 52 kDa

Observed band size: 65 kDa

false

Exposure time: 180s

Supplier Data

Western blot - Anti-Smad2 (phospho S250) antibody [EPR26263-58] (BSA and Azide free) (AB300080)

This data was developed using ab300079, the same antibody clone in a different buffer formulation.

5% NFDM/TBST was used as blocking and diluting buffer.

Bands around and below the 50-kDa marker could be degradation fragments.

All lanes:

Western blot - Anti-Smad2 (phospho S250) antibody [EPR26263-58] (<a href='/en-us/products/primary-antibodies/smad2-phospho-s250-antibody-epr26263-58-ab300079'>ab300079</a>) at 1/1000 dilution

Lane 1:

Untreated C6 (rat glial tumor cell) whole cell lysate at 20 µg

Lane 2:

C6 treated with 0.2µM PMA for 0.5h whole cell lysate at 20 µg

Secondary

All lanes:

Western blot - Goat Anti-Rabbit IgG H&L (HRP) (<a href='/en-us/products/secondary-antibodies/goat-rabbit-igg-h-l-hrp-ab97051'>ab97051</a>) at 1/100000 dilution

Predicted band size: 52 kDa

Observed band size: 65 kDa

false

Exposure time: 180s

Supplier Data

Western blot - Anti-Smad2 (phospho S250) antibody [EPR26263-58] (BSA and Azide free) (AB300080)

This data was developed using ab300079, the same antibody clone in a different buffer formulation. 5% NFDM/TBST was used as blocking and diluting buffer. Bands around and below the 50-kDa marker could be degradation fragments.

All lanes:

Western blot - Anti-Smad2 (phospho S250) antibody [EPR26263-58] (<a href='/en-us/products/primary-antibodies/smad2-phospho-s250-antibody-epr26263-58-ab300079'>ab300079</a>) at 1/1000 dilution

Lane 1:

Untreated C6 (rat glial tumor cell) whole cell lysate at 20 µg

Lane 2:

C6 treated with 0.2µM PMA for 0.5h whole cell lysate at 20 µg

Secondary

All lanes:

Western blot - Goat Anti-Rabbit IgG H&L (HRP) (<a href='/en-us/products/secondary-antibodies/goat-rabbit-igg-h-l-hrp-ab97051'>ab97051</a>) at 1/100000 dilution

Predicted band size: 52 kDa

Observed band size: 65 kDa

false

Exposure time: 180s

Supplier Data

Western blot - Anti-Smad2 (phospho S250) antibody [EPR26263-58] (BSA and Azide free) (AB300080)

This data was developed using ab300079, the same antibody clone in a different buffer formulation. 5% NFDM/TBST was used as blocking and diluting buffer.

All lanes:

Western blot - Anti-Smad2 (phospho S250) antibody [EPR26263-58] (<a href='/en-us/products/primary-antibodies/smad2-phospho-s250-antibody-epr26263-58-ab300079'>ab300079</a>) at 1/1000 dilution

Lane 1:

Untreated NIH/3T3 (Mouse embryonic fibroblast) whole cell lysate at 20 µg

Lane 2:

NIH/3T3 treated with 0.2µM PMA for 0.5h whole cell lysate at 20 µg

Secondary

All lanes:

Western blot - Goat Anti-Rabbit IgG H&L (HRP) (<a href='/en-us/products/secondary-antibodies/goat-rabbit-igg-h-l-hrp-ab97051'>ab97051</a>) at 1/100000 dilution

Predicted band size: 52 kDa

Observed band size: 65 kDa

false

Exposure time: 180s

Supplier Data

Dot Blot - Anti-Smad2 (phospho S250) antibody [EPR26263-58] (BSA and Azide free) (AB300080)

This data was developed using ab300079, the same antibody clone in a different buffer formulation. Dot blot analysis of Smad2 (phospho S250) using ab300079 at 1 : 1000 (0.54 µg/ml), followed by a Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated (ab97051) at 1 : 100,000 dilution. Lane 1 : Smad2 (phospho S245+S250+S255) peptide a Lane 2 : Smad2 (phospho S245+S250+S255) peptide b Lane 3 : Smad2 (phospho S245+S250) peptide Lane 4 : Smad2 (phospho S245+S255) peptide Lane 5 : Smad2 (phospho S250+S255) peptide Lane 6 : Smad2 (phospho S245) peptide Lane 7 : Smad2 (phospho S250) peptide Lane 8 : Smad2 (phospho S255) peptide Lane 9 : Smad2 non-phospho peptide Exposure time : 3 minutes Blocking and diluting buffer and concentration : 5% NFDM/TBST

Supplier Data

Dot Blot - Anti-Smad2 (phospho S250) antibody [EPR26263-58] (BSA and Azide free) (AB300080)

This data was developed using ab300079, the same antibody clone in a different buffer formulation.

Dot blot analysis of Smad2 (phospho S250) using ab300079 at 1 : 1000 (0.54 μg/ml), followed by a Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated (ab97051) at 1 : 100,000 dilution.

Lane 1 : Smad2 (phospho S245+S250+S255) peptide a
Lane 2 : Smad2 (phospho S245+S250+S255) peptide b
Lane 3 : Smad2 (phospho S245+S250) peptide
Lane 4 : Smad2 (phospho S245+S255) peptide
Lane 5 : Smad2 (phospho S250+S255) peptide
Lane 6 : Smad2 (phospho S245) peptide

Lane 7 : Smad2 (phospho S250) peptide
Lane 8 : Smad2 (phospho S255) peptide
Lane 9 : Smad2 non-phospho peptide

Exposure time : 3 minutes

Blocking and diluting buffer and concentration : 5% NFDM/TBST

Unconjugated

Anti-Smad2 (phospho S250) antibody [EPR26263-58]

Key facts

Host species

Rabbit

Clonality

Monoclonal

Clone number

EPR26263-58

Isotype

IgG

Carrier free

Yes

Reacts with

Human, Mouse, Rat

Applications

Dot, WB, ICC/IF, IP

applications

Immunogen

The exact immunogen used to generate this antibody is proprietary information.

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Reactivity data

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Product details

Patented technology
Our RabMAb^® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb^® patents.

Conjugation ready
Our carrier-free antibodies are typically supplied in a PBS-only formulation, purified and free of BSA, sodium azide and glycerol. This conjugation-ready format is designed for use with fluorochromes, metal isotopes, oligonucleotides, and enzymes, which makes them ideal for antibody labelling, functional and cell-based assays, flow-based assays (e.g. mass cytometry) and Multiplex Imaging applications.

Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with 1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.

Compatibility
This product is compatible with the Maxpar^® Antibody Labeling Kit from Fluidigm, without the need for antibody preparation. Maxpar^® is a trademark of Fluidigm Canada Inc.

What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:

- High batch-to-batch consistency and reproducibility
- Improved sensitivity and specificity
- Long-term security of supply
- Animal-free batch production

For more information, read more on recombinant antibodies.

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Properties and storage information

Form

Liquid

Purification technique

Affinity purification Protein A

Storage buffer

pH: 7.2 - 7.4 Constituents: PBS

Shipped at conditions

Blue Ice

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

+4°C

Aliquoting information

Upon delivery aliquot

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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

Smad2 also known as Mothers against decapentaplegic homolog 2 (MAD2) or MADR2 is a signaling protein involved in the transforming growth factor-beta (TGF-β) receptor pathway. Smad2 has a molecular mass of approximately 58 kDa and expresses in various tissues including epithelial mesenchymal and endothelial cells. Smad2 undergoes phosphorylation on serine residues in response to TGF-β signaling converting it into phosphorylated forms often referred to as p-Smad2 or phospho-Smad2. These phosphorylated forms are critical for the relay of signals from the cell surface to the nucleus.

Biological function summary

Smad2 acts as an intracellular mediator for TGF-β signaling a pathway important for regulating cell proliferation differentiation and apoptosis. Smad2 typically functions as part of a heteromeric complex with Smad4 another key player in TGF-β signaling. Upon activation phosphorylated Smad2 combines with Smad4 to form a complex that translocates into the nucleus. This complex then binds to specific DNA sequences to regulate the transcription of target genes involved in processes such as cell growth inhibition and extracellular matrix production.

Pathways

Smad2 is integral to the TGF-β and activin receptor signaling pathways. These pathways are essential in controlling cell growth and immune responses. Smad2 interacts with other proteins such as Smad3 in addition to Smad4 to modulate gene expression effectively. The interaction between Smad2 and these proteins ensures precise control of cellular responses to external stimuli emphasizing its pivotal role in maintaining cellular homeostasis.

Smad2 correlates with various pathological conditions including fibrosis and cancer. Aberrant Smad2 signaling can contribute to the development of these diseases as excessive TGF-β signaling promotes fibrotic tissue deposition and tumor progression. Smad2 connects with other proteins like Smad3 in these pathological contexts both acting as mediators of abnormal cellular behaviors. Understanding the regulatory mechanisms of Smad2 can help develop therapeutic strategies against disorders linked to dysregulated TGF-β signaling.

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Product protocols

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Target data

Receptor-regulated SMAD (R-SMAD) that is an intracellular signal transducer and transcriptional modulator activated by TGF-beta (transforming growth factor) and activin type 1 receptor kinases. Binds the TRE element in the promoter region of many genes that are regulated by TGF-beta and, on formation of the SMAD2/SMAD4 complex, activates transcription. Promotes TGFB1-mediated transcription of odontoblastic differentiation genes in dental papilla cells (By similarity). Positively regulates PDPK1 kinase activity by stimulating its dissociation from the 14-3-3 protein YWHAQ which acts as a negative regulator. May act as a tumor suppressor in colorectal carcinoma (PubMed : 8752209).

See full target information SMAD2 phospho S250

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Anti-Smad2 (phospho S250) antibody [EPR26263-58] (BSA and Azide free)

Immunocytochemistry/ Immunofluorescence - Anti-Smad2 (phospho S250) antibody [EPR26263-58] (BSA and Azide free) (AB300080)

Immunocytochemistry/ Immunofluorescence - Anti-Smad2 (phospho S250) antibody [EPR26263-58] (BSA and Azide free) (AB300080)

Immunoprecipitation - Anti-Smad2 (phospho S250) antibody [EPR26263-58] (BSA and Azide free) (AB300080)

Immunoprecipitation - Anti-Smad2 (phospho S250) antibody [EPR26263-58] (BSA and Azide free) (AB300080)

Immunoprecipitation - Anti-Smad2 (phospho S250) antibody [EPR26263-58] (BSA and Azide free) (AB300080)

Immunoprecipitation - Anti-Smad2 (phospho S250) antibody [EPR26263-58] (BSA and Azide free) (AB300080)

Western blot - Anti-Smad2 (phospho S250) antibody [EPR26263-58] (BSA and Azide free) (AB300080)

Western blot - Anti-Smad2 (phospho S250) antibody [EPR26263-58] (BSA and Azide free) (AB300080)

Western blot - Anti-Smad2 (phospho S250) antibody [EPR26263-58] (BSA and Azide free) (AB300080)

Western blot - Anti-Smad2 (phospho S250) antibody [EPR26263-58] (BSA and Azide free) (AB300080)

Western blot - Anti-Smad2 (phospho S250) antibody [EPR26263-58] (BSA and Azide free) (AB300080)

Western blot - Anti-Smad2 (phospho S250) antibody [EPR26263-58] (BSA and Azide free) (AB300080)

Western blot - Anti-Smad2 (phospho S250) antibody [EPR26263-58] (BSA and Azide free) (AB300080)

Western blot - Anti-Smad2 (phospho S250) antibody [EPR26263-58] (BSA and Azide free) (AB300080)

Dot Blot - Anti-Smad2 (phospho S250) antibody [EPR26263-58] (BSA and Azide free) (AB300080)

Dot Blot - Anti-Smad2 (phospho S250) antibody [EPR26263-58] (BSA and Azide free) (AB300080)

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