AB188334

Anti-Smad2 (phospho S255) antibody [EPR2856(N)]

Name: Anti-Smad2 (phospho S255) antibody [EPR2856(N)] (ab188334) | Abcam
Brand: Abcam Limited
SKU: AB188334
Price: 215 USD
Availability: InStock
Rating: 5 (2 reviews)

20ul selling size
Recombinant
Advanced Validation
RabMAb
What is this?

(2 Reviews)

(86 Publications)

Anti-Smad2 (phospho S255) antibody [EPR2856(N)] (ab188334) is a rabbit monoclonal antibody detecting Smad2 in Western Blot, IP, IHC-P, Dot Blot. Suitable for Human, Mouse, Rat.

- Biophysical QC for unrivalled batch-batch consistency
- Over 60 publications

View Alternative Names

MADH2, MADR2, SMAD2, SMAD family member 2, SMAD 2, hSMAD2, JV18-1, Mad-related protein 2, Mothers against decapentaplegic homolog 2, hMAD-2, MAD homolog 2, Mothers against DPP homolog 2

8 Images

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Smad2 (phospho S255) antibody [EPR2856(N)] (AB188334)

IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Smad2 (phospho S255) antibody [EPR2856(N)] (AB188334)

Immunohistochemical analysis of formalin fixed paraffin embedded Human endometrium labeling Smad2 (phospho S255) with ab188334 at 1/100 dilution and HRP Polymer for Rabbit IgG. Counterstained with Hematoxylin.

Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Smad2 (phospho S255) antibody [EPR2856(N)] (AB188334)

Immunohistochemical analysis of formalin fixed paraffin embedded Human transitional cell carcinoma of bladder labeling Smad2 (phospho S255) with ab188334 at 1/100 dilution and HRP Polymer for Rabbit IgG. Counterstained with Hematoxylin.

Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

Supplier Data

Immunoprecipitation - Anti-Smad2 (phospho S255) antibody [EPR2856(N)] (AB188334)

Immunoprecipitation of Hela cells treated with Okadaic acis and Calyculin A (Lane 1) or PBS (Lane 2) labeling Smad2 (phospho S255) with ab188334 at 1/50 dilution and Anti-Rabbit IgG (HRP), specific to the non-reduced form of IgG at 1/1500 dilution

All lanes:

Immunoprecipitation - Anti-Smad2 (phospho S255) antibody [EPR2856(N)] (ab188334)

Predicted band size: 52 kDa

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ChIC/CUT&RUN sequencing - Anti-Smad2 (phospho S255) antibody [EPR2856(N)] (AB188334)

ChIC/CUT&RUN-seq

Supplier Data

ChIC/CUT&RUN sequencing - Anti-Smad2 (phospho S255) antibody [EPR2856(N)] (AB188334)

ChIC/CUT&RUN was performed using a pAG-MNAse at a final concentration of 700 ng/µL, 2 x 10^5 HaCaT (human skin keratinocyte) cells and 5µg of ab188334 [EPR2856(N)]. The resulting DNA was sequenced on the Illumina NovaSeq 6000 to a depth of 10 million reads. The negative IgG control ab172730 is also shown. Additional screenshots of mapped reads can be found in the Protocol booklet in the Product Protocol section. The University of Geneva owns patents relevant to ChIC (Chromatin Immuno-Cleavage) methods.

Unknown

Western blot - Anti-Smad2 (phospho S255) antibody [EPR2856(N)] (AB188334)

All lanes:

Western blot - Anti-Smad2 (phospho S255) antibody [EPR2856(N)] (ab188334) at 1/10000 dilution

Lane 1:

PC-12 (Rat adrenal gland pheochromocytoma) whole cell lysate at 15 µg

Lane 2:

PC-12 (Rat adrenal gland pheochromocytoma) whole cell lysate, Then the membrane was incubated with phosphatase at 15 µg

Secondary

All lanes:

Western blot - Goat Anti-Rabbit IgG H&L (HRP) (<a href='/en-us/products/secondary-antibodies/goat-rabbit-igg-h-l-hrp-ab97051'>ab97051</a>) at 1/20000 dilution

Predicted band size: 52 kDa

false

Unknown

Western blot - Anti-Smad2 (phospho S255) antibody [EPR2856(N)] (AB188334)

All lanes:

Western blot - Anti-Smad2 (phospho S255) antibody [EPR2856(N)] (ab188334) at 1/10000 dilution

Lane 1:

RAW 264.7 (Mouse Abelson murine leukemia virus-induced tumor macrophage) whole cell lysate at 15 µg

Lane 2:

RAW 264.7 (Mouse Abelson murine leukemia virus-induced tumor macrophage) whole cell lysate. Then the membrane was incubated with phosphatase at 15 µg

Secondary

All lanes:

Western blot - Goat Anti-Rabbit IgG H&L (HRP) (<a href='/en-us/products/secondary-antibodies/goat-rabbit-igg-h-l-hrp-ab97051'>ab97051</a>) at 1/20000 dilution

Predicted band size: 52 kDa

false

Supplier Data

Western blot - Anti-Smad2 (phospho S255) antibody [EPR2856(N)] (AB188334)

All lanes:

Western blot - Anti-Smad2 (phospho S255) antibody [EPR2856(N)] (ab188334) at 1/10000 dilution

Lane 1:

Hela treated with Okadaic acid and Calyculin A at 10 µg

Lane 2:

Hela treated with Okadaic acid and Calyculin A , then treated with Lambda phosphorylase at 10 µg

Lane 3:

Untreated Hela at 10 µg

Secondary

All lanes:

Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/1000 dilution

Predicted band size: 52 kDa

false

Lab

Dot Blot - Anti-Smad2 (phospho S255) antibody [EPR2856(N)] (AB188334)

Dot blot analysis of Smad2 (S255) phospho peptide (Lane 1), Smad2 non-phospho peptide (Lane 2), labelling Smad2 (S255) phospho peptide with ab188334 at a dilution of 1 : 1000 dilution (1.365ug/ml). A Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated (ab97051) was used as the secondary antibody at a dilution of 1 : 20,000 dilution. Blocking buffer : 5% NFDM/TBST. Dilution buffer : 5% NFDM /TBST .

Carrier free

Anti-Smad2 (phospho S255) antibody [EPR2856(N)] - BSA and Azide free
519 Alexa Fluor® 488

Alexa Fluor® 488 Anti-Smad2 (phospho S255) antibody [EPR2856(N)]
565 Alexa Fluor® 555

Alexa Fluor® 555 Anti-Smad2 (phospho S255) antibody [EPR2856(N)]
603 Alexa Fluor® 568

Alexa Fluor® 568 Anti-Smad2 (phospho S255) antibody [EPR2856(N)]
617 Alexa Fluor® 594

Alexa Fluor® 594 Anti-Smad2 (phospho S255) antibody [EPR2856(N)]
665 Alexa Fluor® 647

Alexa Fluor® 647 Anti-Smad2 (phospho S255) antibody [EPR2856(N)]
775 Alexa Fluor® 750

Alexa Fluor® 750 Anti-Smad2 (phospho S255) antibody [EPR2856(N)]

Key facts

Host species

Rabbit

Clonality

Monoclonal

Clone number

EPR2856(N)

Isotype

IgG

Carrier free

Reacts with

Mouse, Rat, Human

Applications

WB, ChIC/CUT&RUN-seq, Dot, IP, IHC-P

applications

Immunogen

The exact immunogen used to generate this antibody is proprietary information.

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "ChICCUTRUNseq" : {"fullname" : "ChIC/CUT&RUN sequencing", "shortname":"ChIC/CUT&RUN-seq"}, "IP" : {"fullname" : "Immunoprecipitation", "shortname":"IP"}, "Dot" : {"fullname" : "Dot Blot", "shortname":"Dot"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "ChICCUTRUNseq-species-checked": "testedAndGuaranteed", "ChICCUTRUNseq-species-dilution-info": "5 µg", "ChICCUTRUNseq-species-notes": "", "IP-species-checked": "testedAndGuaranteed", "IP-species-dilution-info": "1/50 - 1/70", "IP-species-notes": "", "Dot-species-checked": "guaranteed", "Dot-species-dilution-info": "", "Dot-species-notes": "", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/1000 - 1/10000", "WB-species-notes": "", "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "1/50 - 1/100", "IHCP-species-notes": "" }, "Mouse": { "ChICCUTRUNseq-species-checked": "guaranteed", "ChICCUTRUNseq-species-dilution-info": "", "ChICCUTRUNseq-species-notes": "", "IP-species-checked": "guaranteed", "IP-species-dilution-info": "", "IP-species-notes": "", "Dot-species-checked": "guaranteed", "Dot-species-dilution-info": "", "Dot-species-notes": "", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/1000 - 1/10000", "WB-species-notes": "", "IHCP-species-checked": "guaranteed", "IHCP-species-dilution-info": "", "IHCP-species-notes": "" }, "Rat": { "ChICCUTRUNseq-species-checked": "guaranteed", "ChICCUTRUNseq-species-dilution-info": "", "ChICCUTRUNseq-species-notes": "", "IP-species-checked": "guaranteed", "IP-species-dilution-info": "", "IP-species-notes": "", "Dot-species-checked": "guaranteed", "Dot-species-dilution-info": "", "Dot-species-notes": "", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/1000 - 1/10000", "WB-species-notes": "", "IHCP-species-checked": "guaranteed", "IHCP-species-dilution-info": "", "IHCP-species-notes": "" }, "Synthetic peptide - Human": { "ChICCUTRUNseq-species-checked": "notRecommended", "ChICCUTRUNseq-species-dilution-info": "", "ChICCUTRUNseq-species-notes": "", "IP-species-checked": "notRecommended", "IP-species-dilution-info": "", "IP-species-notes": "", "Dot-species-checked": "testedAndGuaranteed", "Dot-species-dilution-info": "", "Dot-species-notes": "", "WB-species-checked": "notRecommended", "WB-species-dilution-info": "", "WB-species-notes": "", "IHCP-species-checked": "notRecommended", "IHCP-species-dilution-info": "", "IHCP-species-notes": "" } } }

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Product details

What is this antibody validated in?
Anti-Smad2 (phospho S255) antibody [EPR2856(N)] (ab188334) is a rabbit recombinant monoclonal antibody and is validated for use in Western Blot (WB), Immunoprecipitation (IP), Immunohistochemistry (IHC-P), Dot Blot in Human, Mouse, Rat samples.

What is the molecular weight of Smad2?
Anti-Smad2 (phospho S255) [EPR2856(N)] (ab188334) specifically detects a band for Smad2 (UniProt: Q15796) at a molecular weight of 52kDa.

Trusted by the scientific community
Anti-Smad2 (phospho S255) [EPR2856(N)] (ab188334) was first used in a scientific publication in 2014 and has been cited over 60 times in peer-reviewed journals.

Trial sizes available!
Test your antibody or perform pre-screening before committing to a larger quantity. Sold in 10µl. Discover our selection of trial-size antibodies.

Other related products
We have a range of other formats of antibody clone [EPR2856(N)] also available for your convenience: ab188334, Carrier free - ab219598, Alexa Fluor® 488 - ab310955, Alexa Fluor® 647 - ab311072, Alexa Fluor® 594 - ab311659, Alexa Fluor® 568 - ab312932, Alexa Fluor® 555 - ab313144, Alexa Fluor® 750 - ab321306

Patented technology
Our RabMAb^® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb^® patents.

What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:

- High batch-to-batch consistency and reproducibility
- Improved sensitivity and specificity
- Long-term security of supply
- Animal-free batch production

For more information, read more on recombinant antibodies.

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Properties and storage information

Form

Liquid

Purification technique

Affinity purification Protein A

Storage buffer

pH: 7.2 - 7.4 Preservative: 0.01% Sodium azide Constituents: PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA

Shipped at conditions

Blue Ice

Appropriate short-term storage duration

1-2 weeks

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

-20°C

Aliquoting information

Upon delivery aliquot

Storage information

Avoid freeze / thaw cycle

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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

Smad2 also known as Mothers against decapentaplegic homolog 2 (MAD2) or MADR2 is a signaling protein involved in the transforming growth factor-beta (TGF-Β) receptor pathway. Smad2 has a molecular mass of approximately 58 kDa and expresses in various tissues including epithelial mesenchymal and endothelial cells. Smad2 undergoes phosphorylation on serine residues in response to TGF-Β signaling converting it into phosphorylated forms often referred to as p-Smad2 or phospho-Smad2. These phosphorylated forms are critical for the relay of signals from the cell surface to the nucleus.

Biological function summary

Smad2 acts as an intracellular mediator for TGF-Β signaling a pathway important for regulating cell proliferation differentiation and apoptosis. Smad2 typically functions as part of a heteromeric complex with Smad4 another key player in TGF-Β signaling. Upon activation phosphorylated Smad2 combines with Smad4 to form a complex that translocates into the nucleus. This complex then binds to specific DNA sequences to regulate the transcription of target genes involved in processes such as cell growth inhibition and extracellular matrix production.

Pathways

Smad2 is integral to the TGF-Β and activin receptor signaling pathways. These pathways are essential in controlling cell growth and immune responses. Smad2 interacts with other proteins such as Smad3 in addition to Smad4 to modulate gene expression effectively. The interaction between Smad2 and these proteins ensures precise control of cellular responses to external stimuli emphasizing its pivotal role in maintaining cellular homeostasis.

Smad2 correlates with various pathological conditions including fibrosis and cancer. Aberrant Smad2 signaling can contribute to the development of these diseases as excessive TGF-Β signaling promotes fibrotic tissue deposition and tumor progression. Smad2 connects with other proteins like Smad3 in these pathological contexts both acting as mediators of abnormal cellular behaviors. Understanding the regulatory mechanisms of Smad2 can help develop therapeutic strategies against disorders linked to dysregulated TGF-Β signaling.

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:

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Target data

Receptor-regulated SMAD (R-SMAD) that is an intracellular signal transducer and transcriptional modulator activated by TGF-beta (transforming growth factor) and activin type 1 receptor kinases. Binds the TRE element in the promoter region of many genes that are regulated by TGF-beta and, on formation of the SMAD2/SMAD4 complex, activates transcription. Promotes TGFB1-mediated transcription of odontoblastic differentiation genes in dental papilla cells (By similarity). Positively regulates PDPK1 kinase activity by stimulating its dissociation from the 14-3-3 protein YWHAQ which acts as a negative regulator. May act as a tumor suppressor in colorectal carcinoma (PubMed : 8752209).

See full target information SMAD2 pS255

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Publications (86)

Recent publications for all applications. Explore the full list and refine your search

Cancer cell international 25:179 PubMed40380175

2025

Fibrogenesis-driven tumor progression in clear cell renal cell carcinoma: prognostic, therapeutic implications and the dual role of neuropilin-1.

Applications

Unspecified application

Species

Unspecified reactive species

Kai Wang,Xihao Shen,Jiyue Wu,Qing Bi,Zihao Gao,Zejia Sun,Wei Wang

Biomedicines 13: PubMed40299436

2025

TGF-β1 Improves Nerve Regeneration and Functional Recovery After Sciatic Nerve Injury by Alleviating Inflammation.

Applications

Unspecified application

Species

Unspecified reactive species

Maorong Jiang,Zihan Ding,Yuxiao Huang,Taoran Jiang,Yiming Xia,Dandan Gu,Xi Gu,Huiyuan Bai,Dengbing Yao

Frontiers in pharmacology 16:1565156 PubMed40271070

2025

TRPC3 contributes to cyclophosphamide-induced cystitis progression by enhancing bladder fibrosis through activation of the TGF-β/smad pathway.

Applications

Unspecified application

Species

Unspecified reactive species

Ruixiang Luo,Wenshuang Li,Junlong Huang,Zheng Liu,Chi Zhang,Honglu Ding,Jialiang Chen

BMC medicine 23:89 PubMed39948553

2025

Cryoablation-induced modulation of Treg cells and the TGF-β pathway in lung adenocarcinoma: implications for increased antitumor immunity.

Applications

Unspecified application

Species

Unspecified reactive species

Shicheng Lin,Dianna Liu,Tianyu Liang,Yaoxue Zhuang,Xiaofan Wang,Shengmao Ma,Quanwang Li,Kaiwen Hu

American journal of translational research 16:7924-7936 PubMed39822499

2025

Heparan sulfate 6-O-sulfotransferase 2 promotes gastric cancer progression by modulating the TGF-β/smad2/3 pathway.

Applications

Unspecified application

Species

Unspecified reactive species

Shuai Wu,Changqin Xu,Weijia Sun,Qianqian Xu,Feifei Zhou,Ruzhen Jia,Hongwei Xu

Science advances 11:eadq4991 PubMed39772668

2025

Tgfβ signaling stimulates glycolysis to promote the genesis of synovial joint interzone in developing mouse embryonic limbs.

Applications

Unspecified application

Species

Unspecified reactive species

Chao Song,Jasmin Koehnken Sawall,Xing Ji,Fangfang Song,Xueyang Liao,Renpeng Peng,Hao Ren,Eiki Koyama,Maurizio Pacifici,Fanxin Long

BMC cardiovascular disorders 24:535 PubMed39367342

2024

Quercetin regulates pulmonary vascular remodeling in pulmonary hypertension by downregulating TGF-β1-Smad2/3 pathway.

Applications

Unspecified application

Species

Unspecified reactive species

Rui-Juan Gao,Nigala Aikeremu,Nan Cao,Chong Chen,Ke-Tao Ma,Li Li,Ai-Mei Zhang,Jun-Qiang Si

Journal of translational medicine 22:445 PubMed38735939

2024

Multi-omics integration highlights the role of ubiquitination in endometriosis fibrosis.

Applications

Unspecified application

Species

Unspecified reactive species

Mengjie Yang,Hong Jiang,Xinyu Ding,Lu Zhang,Huaying Zhang,Jiahao Chen,Lijun Li,Xinqin He,Zhixiong Huang,Qionghua Chen

Cellular and molecular biology (Noisy-le-Grand, France) 70:95-101 PubMed38650149

2024

Ginsenoside Rc alleviates osteoporosis by the TGF-β/Smad signaling pathway.

Applications

Unspecified application

Species

Unspecified reactive species

Shanfu Wang,Bing Xu,Heng Yin,Zhen Hua,Yang Shao,Jianwei Wang

Experimental biology and medicine (Maywood, N.J.) 249:10111 PubMed38510491

2024

miR-539-5p targets BMP2 to regulate Treg activation in B-cell acute lymphoblastic leukemia through TGF-β/Smads/MAPK.

Applications

Unspecified application

Species

Unspecified reactive species

Qingkai Dai,Rui Shi,Ge Zhang,Yuefang Wang,Lei Ye,Luyun Peng,Siqi Guo,Jiajing He,Hao Yang,Yongmei Jiang

View all publications

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chicCutRunSequencingBooklet

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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.

For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com

Anti-Smad2 (phospho S255) antibody [EPR2856(N)]

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Smad2 (phospho S255) antibody [EPR2856(N)] (AB188334)

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Smad2 (phospho S255) antibody [EPR2856(N)] (AB188334)

Immunoprecipitation - Anti-Smad2 (phospho S255) antibody [EPR2856(N)] (AB188334)

ChIC/CUT&RUN sequencing - Anti-Smad2 (phospho S255) antibody [EPR2856(N)] (AB188334)

Western blot - Anti-Smad2 (phospho S255) antibody [EPR2856(N)] (AB188334)

Western blot - Anti-Smad2 (phospho S255) antibody [EPR2856(N)] (AB188334)

Western blot - Anti-Smad2 (phospho S255) antibody [EPR2856(N)] (AB188334)

Dot Blot - Anti-Smad2 (phospho S255) antibody [EPR2856(N)] (AB188334)

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Key facts

Host species

Clonality

Clone number

Isotype

Carrier free

Reacts with

Applications

Immunogen

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Properties and storage information

Form

Purification technique

Storage buffer

Shipped at conditions

Appropriate short-term storage duration

Appropriate short-term storage conditions

Appropriate long-term storage conditions

Aliquoting information

Storage information

Supplementary information

Biological function summary

Pathways

Product protocols

Target data

Publications (86)

Product promise